Experiment: 50% P. fluorescens FW300-N2E2 spent media from growth in 20 mM putrescine
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt Pf6N2E2_3707 and Pf6N2E2_3708 are separated by 335 nucleotides Pf6N2E2_3708 and Pf6N2E2_3709 are separated by 16 nucleotides
Pf6N2E2_3707 - Asp-tRNAAsn/Glu-tRNAGln amidotransferase A subunit and related amidases, at 4,321,380 to 4,322,753
_3707
Pf6N2E2_3708 - predicted biotin regulatory protein BioR (GntR family), at 4,323,089 to 4,324,090
_3708
Pf6N2E2_3709 - Chromosome (plasmid) partitioning protein ParA, at 4,324,107 to 4,324,874
_3709
Position (kb)
4323
4324
4325 Strain fitness (log2 ratio)
-1
0
1
2 at 4322.766 kb on - strand at 4322.772 kb on + strand at 4322.813 kb on + strand at 4322.814 kb on - strand at 4322.831 kb on - strand at 4322.831 kb on - strand at 4322.831 kb on - strand at 4322.868 kb on - strand at 4322.868 kb on - strand at 4322.936 kb on - strand at 4322.939 kb on + strand at 4322.939 kb on + strand at 4322.939 kb on + strand at 4322.940 kb on - strand at 4322.940 kb on - strand at 4322.940 kb on - strand at 4322.944 kb on + strand at 4322.944 kb on + strand at 4322.944 kb on + strand at 4322.944 kb on + strand at 4322.945 kb on - strand at 4322.945 kb on - strand at 4322.945 kb on - strand at 4322.945 kb on - strand at 4322.962 kb on - strand at 4322.967 kb on + strand at 4323.150 kb on + strand at 4323.150 kb on + strand at 4323.150 kb on + strand at 4323.150 kb on + strand at 4323.150 kb on + strand at 4323.151 kb on - strand at 4323.195 kb on + strand, within Pf6N2E2_3708 at 4323.195 kb on + strand, within Pf6N2E2_3708 at 4323.195 kb on + strand, within Pf6N2E2_3708 at 4323.195 kb on + strand, within Pf6N2E2_3708 at 4323.196 kb on - strand, within Pf6N2E2_3708 at 4323.196 kb on - strand, within Pf6N2E2_3708 at 4323.198 kb on + strand, within Pf6N2E2_3708 at 4323.198 kb on + strand, within Pf6N2E2_3708 at 4323.198 kb on + strand, within Pf6N2E2_3708 at 4323.198 kb on + strand, within Pf6N2E2_3708 at 4323.308 kb on + strand, within Pf6N2E2_3708 at 4323.463 kb on + strand, within Pf6N2E2_3708 at 4323.463 kb on + strand, within Pf6N2E2_3708 at 4323.463 kb on + strand, within Pf6N2E2_3708 at 4323.464 kb on - strand, within Pf6N2E2_3708 at 4323.464 kb on - strand, within Pf6N2E2_3708 at 4323.464 kb on - strand, within Pf6N2E2_3708 at 4323.510 kb on - strand, within Pf6N2E2_3708 at 4323.636 kb on - strand, within Pf6N2E2_3708 at 4323.951 kb on + strand, within Pf6N2E2_3708 at 4323.951 kb on + strand, within Pf6N2E2_3708 at 4323.952 kb on - strand, within Pf6N2E2_3708 at 4324.070 kb on + strand at 4324.228 kb on + strand, within Pf6N2E2_3709 at 4324.228 kb on + strand, within Pf6N2E2_3709 at 4324.343 kb on + strand, within Pf6N2E2_3709 at 4324.384 kb on + strand, within Pf6N2E2_3709 at 4324.385 kb on - strand, within Pf6N2E2_3709 at 4324.504 kb on + strand, within Pf6N2E2_3709 at 4324.504 kb on + strand, within Pf6N2E2_3709 at 4324.633 kb on + strand, within Pf6N2E2_3709 at 4324.633 kb on + strand, within Pf6N2E2_3709 at 4324.633 kb on + strand, within Pf6N2E2_3709 at 4324.633 kb on + strand, within Pf6N2E2_3709 at 4324.633 kb on + strand, within Pf6N2E2_3709 at 4324.634 kb on - strand, within Pf6N2E2_3709 at 4324.638 kb on - strand, within Pf6N2E2_3709 at 4324.700 kb on + strand, within Pf6N2E2_3709 at 4324.701 kb on - strand, within Pf6N2E2_3709 at 4324.738 kb on + strand, within Pf6N2E2_3709 at 4324.739 kb on - strand, within Pf6N2E2_3709 at 4324.890 kb on + strand at 4324.890 kb on + strand at 4324.909 kb on + strand at 4324.910 kb on - strand at 4324.910 kb on - strand at 4324.910 kb on - strand at 4324.910 kb on - strand at 4324.910 kb on - strand at 4324.910 kb on - strand at 4324.910 kb on - strand at 4324.910 kb on - strand at 4324.912 kb on + strand at 4324.912 kb on + strand at 4324.913 kb on - strand at 4324.913 kb on - strand at 4324.913 kb on - strand at 4324.929 kb on + strand at 4324.929 kb on + strand at 4324.929 kb on + strand at 4324.930 kb on - strand at 4324.933 kb on + strand at 4324.933 kb on + strand at 4324.933 kb on + strand at 4324.934 kb on - strand at 4324.954 kb on - strand at 4324.954 kb on - strand
Per-strain Table
Position Strand Gene LocusTag Fraction 50% P. fluorescens FW300-N2E2 spent media from growth in 20 mM putrescine remove 4,322,766 - +0.4 4,322,772 + -0.0 4,322,813 + -0.2 4,322,814 - -0.6 4,322,831 - +0.5 4,322,831 - +0.2 4,322,831 - +0.2 4,322,868 - -0.7 4,322,868 - -1.2 4,322,936 - -0.2 4,322,939 + +0.4 4,322,939 + +1.1 4,322,939 + +0.9 4,322,940 - +0.8 4,322,940 - +0.1 4,322,940 - +1.1 4,322,944 + +0.4 4,322,944 + +1.4 4,322,944 + +0.4 4,322,944 + +1.4 4,322,945 - +0.4 4,322,945 - -0.7 4,322,945 - +0.4 4,322,945 - +0.4 4,322,962 - +0.7 4,322,967 + +0.4 4,323,150 + +1.7 4,323,150 + +0.1 4,323,150 + -0.0 4,323,150 + -0.5 4,323,150 + +0.2 4,323,151 - -0.8 4,323,195 + Pf6N2E2_3708 0.11 -0.5 4,323,195 + Pf6N2E2_3708 0.11 -0.1 4,323,195 + Pf6N2E2_3708 0.11 +2.2 4,323,195 + Pf6N2E2_3708 0.11 +0.5 4,323,196 - Pf6N2E2_3708 0.11 -0.7 4,323,196 - Pf6N2E2_3708 0.11 -0.6 4,323,198 + Pf6N2E2_3708 0.11 +0.4 4,323,198 + Pf6N2E2_3708 0.11 -0.5 4,323,198 + Pf6N2E2_3708 0.11 +0.7 4,323,198 + Pf6N2E2_3708 0.11 -0.7 4,323,308 + Pf6N2E2_3708 0.22 -1.5 4,323,463 + Pf6N2E2_3708 0.37 -1.6 4,323,463 + Pf6N2E2_3708 0.37 +0.3 4,323,463 + Pf6N2E2_3708 0.37 +1.7 4,323,464 - Pf6N2E2_3708 0.37 -1.6 4,323,464 - Pf6N2E2_3708 0.37 +1.4 4,323,464 - Pf6N2E2_3708 0.37 -1.6 4,323,510 - Pf6N2E2_3708 0.42 -1.3 4,323,636 - Pf6N2E2_3708 0.55 -0.9 4,323,951 + Pf6N2E2_3708 0.86 -0.6 4,323,951 + Pf6N2E2_3708 0.86 -1.4 4,323,952 - Pf6N2E2_3708 0.86 -0.2 4,324,070 + -0.4 4,324,228 + Pf6N2E2_3709 0.16 -0.0 4,324,228 + Pf6N2E2_3709 0.16 +1.7 4,324,343 + Pf6N2E2_3709 0.31 -0.9 4,324,384 + Pf6N2E2_3709 0.36 +0.1 4,324,385 - Pf6N2E2_3709 0.36 +0.9 4,324,504 + Pf6N2E2_3709 0.52 +0.4 4,324,504 + Pf6N2E2_3709 0.52 +0.7 4,324,633 + Pf6N2E2_3709 0.68 +1.5 4,324,633 + Pf6N2E2_3709 0.68 +0.4 4,324,633 + Pf6N2E2_3709 0.68 +0.1 4,324,633 + Pf6N2E2_3709 0.68 +1.2 4,324,633 + Pf6N2E2_3709 0.68 +0.2 4,324,634 - Pf6N2E2_3709 0.69 +0.1 4,324,638 - Pf6N2E2_3709 0.69 +0.8 4,324,700 + Pf6N2E2_3709 0.77 -0.0 4,324,701 - Pf6N2E2_3709 0.77 +0.4 4,324,738 + Pf6N2E2_3709 0.82 +1.1 4,324,739 - Pf6N2E2_3709 0.82 +0.2 4,324,890 + +1.1 4,324,890 + +0.7 4,324,909 + +1.2 4,324,910 - -0.2 4,324,910 - -0.6 4,324,910 - +0.2 4,324,910 - +0.6 4,324,910 - -0.0 4,324,910 - -0.1 4,324,910 - -0.9 4,324,910 - +0.4 4,324,912 + +0.4 4,324,912 + +0.4 4,324,913 - +0.7 4,324,913 - +0.4 4,324,913 - +0.4 4,324,929 + +1.1 4,324,929 + -0.4 4,324,929 + +0.0 4,324,930 - +1.4 4,324,933 + -0.5 4,324,933 + +0.4 4,324,933 + +0.2 4,324,934 - -0.6 4,324,954 - +0.4 4,324,954 - -0.0
Or see this region's nucleotide sequence