Experiment: 50% P. fluorescens FW300-N2E2 spent media from growth in 20 mM putrescine
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt Pf6N2E2_3639 and Pf6N2E2_3640 are separated by 102 nucleotides Pf6N2E2_3640 and Pf6N2E2_3641 overlap by 10 nucleotides Pf6N2E2_3641 and Pf6N2E2_3642 are separated by 214 nucleotides
Pf6N2E2_3639 - 3',5'-cyclic-nucleotide phosphodiesterase (EC 3.1.4.17), at 4,239,075 to 4,239,911
_3639
Pf6N2E2_3640 - FIG074102: hypothetical protein, at 4,240,014 to 4,240,466
_3640
Pf6N2E2_3641 - ADP-ribose pyrophosphatase (EC 3.6.1.13), at 4,240,457 to 4,241,074
_3641
Pf6N2E2_3642 - lipoprotein, putative, at 4,241,289 to 4,242,035
_3642
Position (kb)
4240
4241 Strain fitness (log2 ratio)
-2
-1
0
1
2 at 4239.032 kb on - strand at 4239.032 kb on - strand at 4239.032 kb on - strand at 4239.032 kb on - strand at 4239.036 kb on - strand at 4239.036 kb on - strand at 4239.057 kb on + strand at 4239.058 kb on - strand at 4239.058 kb on - strand at 4239.058 kb on - strand at 4239.058 kb on - strand at 4239.058 kb on - strand at 4239.058 kb on - strand at 4239.058 kb on - strand at 4239.058 kb on - strand at 4239.061 kb on + strand at 4239.062 kb on - strand at 4239.062 kb on - strand at 4239.062 kb on - strand at 4239.062 kb on - strand at 4239.095 kb on - strand at 4239.095 kb on - strand at 4239.095 kb on - strand at 4239.105 kb on - strand at 4239.879 kb on + strand at 4239.879 kb on + strand at 4239.880 kb on - strand at 4239.880 kb on - strand at 4239.880 kb on - strand at 4239.880 kb on - strand at 4239.937 kb on + strand at 4239.937 kb on + strand at 4239.937 kb on + strand at 4239.937 kb on + strand at 4239.938 kb on - strand at 4239.938 kb on - strand at 4239.938 kb on - strand at 4239.938 kb on - strand at 4239.938 kb on - strand at 4240.001 kb on - strand at 4240.132 kb on + strand, within Pf6N2E2_3640 at 4240.132 kb on + strand, within Pf6N2E2_3640 at 4240.132 kb on + strand, within Pf6N2E2_3640 at 4240.132 kb on + strand, within Pf6N2E2_3640 at 4240.132 kb on + strand, within Pf6N2E2_3640 at 4240.132 kb on + strand, within Pf6N2E2_3640 at 4240.132 kb on + strand, within Pf6N2E2_3640 at 4240.133 kb on - strand, within Pf6N2E2_3640 at 4240.133 kb on - strand, within Pf6N2E2_3640 at 4240.133 kb on - strand, within Pf6N2E2_3640 at 4240.133 kb on - strand, within Pf6N2E2_3640 at 4240.133 kb on - strand, within Pf6N2E2_3640 at 4240.133 kb on - strand, within Pf6N2E2_3640 at 4240.195 kb on + strand, within Pf6N2E2_3640 at 4240.196 kb on - strand, within Pf6N2E2_3640 at 4240.196 kb on - strand, within Pf6N2E2_3640 at 4240.196 kb on - strand, within Pf6N2E2_3640 at 4240.270 kb on + strand, within Pf6N2E2_3640 at 4240.393 kb on + strand, within Pf6N2E2_3640 at 4240.394 kb on - strand, within Pf6N2E2_3640 at 4240.394 kb on - strand, within Pf6N2E2_3640 at 4240.466 kb on + strand at 4240.592 kb on - strand, within Pf6N2E2_3641 at 4240.699 kb on - strand, within Pf6N2E2_3641 at 4240.699 kb on - strand, within Pf6N2E2_3641 at 4241.101 kb on + strand at 4241.101 kb on + strand at 4241.102 kb on - strand at 4241.102 kb on - strand at 4241.102 kb on - strand at 4241.204 kb on + strand at 4241.204 kb on + strand at 4241.205 kb on - strand at 4241.205 kb on - strand at 4241.205 kb on - strand at 4241.213 kb on + strand at 4241.213 kb on + strand at 4241.214 kb on - strand at 4241.367 kb on + strand, within Pf6N2E2_3642
Per-strain Table
Position Strand Gene LocusTag Fraction 50% P. fluorescens FW300-N2E2 spent media from growth in 20 mM putrescine remove 4,239,032 - +0.3 4,239,032 - +0.3 4,239,032 - +1.6 4,239,032 - +0.3 4,239,036 - +0.5 4,239,036 - -0.1 4,239,057 + +1.9 4,239,058 - +0.8 4,239,058 - -0.3 4,239,058 - -1.2 4,239,058 - +0.3 4,239,058 - +1.3 4,239,058 - +0.7 4,239,058 - +1.6 4,239,058 - +0.3 4,239,061 + +2.7 4,239,062 - +0.8 4,239,062 - +0.8 4,239,062 - +1.0 4,239,062 - -0.9 4,239,095 - -0.9 4,239,095 - +0.3 4,239,095 - +1.3 4,239,105 - +0.9 4,239,879 + +0.3 4,239,879 + +0.9 4,239,880 - +1.1 4,239,880 - +0.9 4,239,880 - -0.5 4,239,880 - -1.7 4,239,937 + +0.3 4,239,937 + +0.1 4,239,937 + +1.1 4,239,937 + -0.1 4,239,938 - -0.2 4,239,938 - -0.4 4,239,938 - +0.0 4,239,938 - +1.2 4,239,938 - -0.2 4,240,001 - +0.3 4,240,132 + Pf6N2E2_3640 0.26 +0.3 4,240,132 + Pf6N2E2_3640 0.26 -0.7 4,240,132 + Pf6N2E2_3640 0.26 -2.0 4,240,132 + Pf6N2E2_3640 0.26 -0.6 4,240,132 + Pf6N2E2_3640 0.26 -0.5 4,240,132 + Pf6N2E2_3640 0.26 +0.7 4,240,132 + Pf6N2E2_3640 0.26 +0.1 4,240,133 - Pf6N2E2_3640 0.26 +0.3 4,240,133 - Pf6N2E2_3640 0.26 +0.3 4,240,133 - Pf6N2E2_3640 0.26 -0.7 4,240,133 - Pf6N2E2_3640 0.26 -0.7 4,240,133 - Pf6N2E2_3640 0.26 -0.2 4,240,133 - Pf6N2E2_3640 0.26 +0.1 4,240,195 + Pf6N2E2_3640 0.40 -0.7 4,240,196 - Pf6N2E2_3640 0.40 -2.0 4,240,196 - Pf6N2E2_3640 0.40 -0.3 4,240,196 - Pf6N2E2_3640 0.40 -1.0 4,240,270 + Pf6N2E2_3640 0.57 -0.7 4,240,393 + Pf6N2E2_3640 0.84 -0.0 4,240,394 - Pf6N2E2_3640 0.84 -1.7 4,240,394 - Pf6N2E2_3640 0.84 +0.3 4,240,466 + -1.2 4,240,592 - Pf6N2E2_3641 0.22 +0.3 4,240,699 - Pf6N2E2_3641 0.39 +0.8 4,240,699 - Pf6N2E2_3641 0.39 +1.6 4,241,101 + +1.7 4,241,101 + +0.5 4,241,102 - +1.3 4,241,102 - +2.2 4,241,102 - +0.9 4,241,204 + +0.1 4,241,204 + +0.8 4,241,205 - +0.3 4,241,205 - +0.3 4,241,205 - +0.2 4,241,213 + +0.3 4,241,213 + +1.1 4,241,214 - +2.7 4,241,367 + Pf6N2E2_3642 0.10 +0.1
Or see this region's nucleotide sequence