Experiment: 50% P. fluorescens FW300-N2E2 spent media from growth in 20 mM putrescine
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt Pf6N2E2_349 and Pf6N2E2_350 are separated by 65 nucleotides Pf6N2E2_350 and Pf6N2E2_351 are separated by 5 nucleotides Pf6N2E2_351 and Pf6N2E2_352 are separated by 203 nucleotides
Pf6N2E2_349 - hypothetical protein, at 415,040 to 415,774
_349
Pf6N2E2_350 - Gfa-like protein, at 415,840 to 416,232
_350
Pf6N2E2_351 - Aldehyde dehydrogenase (EC 1.2.1.3), at 416,238 to 417,686
_351
Pf6N2E2_352 - Opine oxidase subunit B, at 417,890 to 419,071
_352
Position (kb) 416
417
418 Strain fitness (log2 ratio) -1
0
1
2 at 415.347 kb on + strand, within Pf6N2E2_349 at 415.515 kb on + strand, within Pf6N2E2_349 at 415.516 kb on - strand, within Pf6N2E2_349 at 415.542 kb on - strand, within Pf6N2E2_349 at 415.660 kb on + strand, within Pf6N2E2_349 at 415.660 kb on + strand, within Pf6N2E2_349 at 415.772 kb on + strand at 415.773 kb on - strand at 415.806 kb on + strand at 415.807 kb on - strand at 415.807 kb on - strand at 415.807 kb on - strand at 415.807 kb on - strand at 415.865 kb on + strand at 416.131 kb on + strand, within Pf6N2E2_350 at 416.192 kb on - strand, within Pf6N2E2_350 at 416.231 kb on - strand at 416.231 kb on - strand at 416.272 kb on + strand at 416.272 kb on + strand at 416.272 kb on + strand at 416.297 kb on - strand at 416.323 kb on + strand at 416.449 kb on + strand, within Pf6N2E2_351 at 416.584 kb on + strand, within Pf6N2E2_351 at 416.584 kb on + strand, within Pf6N2E2_351 at 416.585 kb on - strand, within Pf6N2E2_351 at 417.212 kb on - strand, within Pf6N2E2_351 at 417.301 kb on + strand, within Pf6N2E2_351 at 417.301 kb on + strand, within Pf6N2E2_351 at 417.301 kb on + strand, within Pf6N2E2_351 at 417.302 kb on - strand, within Pf6N2E2_351 at 417.302 kb on - strand, within Pf6N2E2_351 at 417.330 kb on + strand, within Pf6N2E2_351 at 417.531 kb on + strand, within Pf6N2E2_351 at 417.640 kb on + strand at 417.640 kb on + strand at 417.640 kb on + strand at 417.640 kb on + strand at 417.641 kb on - strand at 417.641 kb on - strand at 417.641 kb on - strand at 417.641 kb on - strand at 417.641 kb on - strand at 417.701 kb on + strand at 417.701 kb on + strand at 417.712 kb on + strand at 417.712 kb on + strand at 417.713 kb on - strand at 417.713 kb on - strand at 417.759 kb on + strand at 417.760 kb on - strand at 417.760 kb on - strand at 417.781 kb on + strand at 417.818 kb on + strand at 417.819 kb on - strand at 417.819 kb on - strand at 418.075 kb on - strand, within Pf6N2E2_352 at 418.075 kb on - strand, within Pf6N2E2_352 at 418.131 kb on + strand, within Pf6N2E2_352 at 418.131 kb on + strand, within Pf6N2E2_352 at 418.132 kb on - strand, within Pf6N2E2_352 at 418.132 kb on - strand, within Pf6N2E2_352 at 418.132 kb on - strand, within Pf6N2E2_352 at 418.317 kb on + strand, within Pf6N2E2_352 at 418.318 kb on - strand, within Pf6N2E2_352 at 418.318 kb on - strand, within Pf6N2E2_352 at 418.335 kb on - strand, within Pf6N2E2_352 at 418.496 kb on + strand, within Pf6N2E2_352 at 418.497 kb on - strand, within Pf6N2E2_352 at 418.653 kb on + strand, within Pf6N2E2_352 at 418.654 kb on - strand, within Pf6N2E2_352
Per-strain Table
Position Strand Gene LocusTag Fraction 50% P. fluorescens FW300-N2E2 spent media from growth in 20 mM putrescine remove 415,347 + Pf6N2E2_349 0.42 +1.0 415,515 + Pf6N2E2_349 0.65 +1.4 415,516 - Pf6N2E2_349 0.65 +0.3 415,542 - Pf6N2E2_349 0.68 -0.3 415,660 + Pf6N2E2_349 0.84 +1.7 415,660 + Pf6N2E2_349 0.84 +0.4 415,772 + -0.0 415,773 - +0.7 415,806 + +1.3 415,807 - +1.1 415,807 - +1.3 415,807 - -0.3 415,807 - +0.8 415,865 + +0.6 416,131 + Pf6N2E2_350 0.74 +0.5 416,192 - Pf6N2E2_350 0.90 +0.4 416,231 - +0.9 416,231 - -0.3 416,272 + -0.5 416,272 + +1.6 416,272 + -0.3 416,297 - -0.3 416,323 + +1.7 416,449 + Pf6N2E2_351 0.15 +1.7 416,584 + Pf6N2E2_351 0.24 +0.1 416,584 + Pf6N2E2_351 0.24 +0.2 416,585 - Pf6N2E2_351 0.24 +0.8 417,212 - Pf6N2E2_351 0.67 +1.3 417,301 + Pf6N2E2_351 0.73 +0.4 417,301 + Pf6N2E2_351 0.73 +0.3 417,301 + Pf6N2E2_351 0.73 -0.3 417,302 - Pf6N2E2_351 0.73 +0.7 417,302 - Pf6N2E2_351 0.73 -0.7 417,330 + Pf6N2E2_351 0.75 +1.3 417,531 + Pf6N2E2_351 0.89 +1.4 417,640 + -0.5 417,640 + +0.7 417,640 + +1.3 417,640 + +0.9 417,641 - +0.8 417,641 - +1.1 417,641 - -1.3 417,641 - +0.7 417,641 - +0.1 417,701 + +0.1 417,701 + -1.3 417,712 + -0.0 417,712 + +0.0 417,713 - -1.3 417,713 - +0.8 417,759 + +1.3 417,760 - -0.3 417,760 - -0.3 417,781 + -0.9 417,818 + +0.7 417,819 - +2.4 417,819 - -0.1 418,075 - Pf6N2E2_352 0.16 +0.7 418,075 - Pf6N2E2_352 0.16 +1.6 418,131 + Pf6N2E2_352 0.20 +1.4 418,131 + Pf6N2E2_352 0.20 +0.5 418,132 - Pf6N2E2_352 0.20 +0.4 418,132 - Pf6N2E2_352 0.20 +0.3 418,132 - Pf6N2E2_352 0.20 +0.7 418,317 + Pf6N2E2_352 0.36 -0.5 418,318 - Pf6N2E2_352 0.36 -0.3 418,318 - Pf6N2E2_352 0.36 -0.3 418,335 - Pf6N2E2_352 0.38 +0.7 418,496 + Pf6N2E2_352 0.51 +0.3 418,497 - Pf6N2E2_352 0.51 -0.3 418,653 + Pf6N2E2_352 0.65 +1.0 418,654 - Pf6N2E2_352 0.65 +0.5
Or see this region's nucleotide sequence