Experiment: 50% P. fluorescens FW300-N2E2 spent media from growth in 20 mM putrescine
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt Pf6N2E2_3327 and Pf6N2E2_3328 are separated by 241 nucleotides Pf6N2E2_3328 and Pf6N2E2_3329 are separated by 97 nucleotides Pf6N2E2_3329 and Pf6N2E2_3330 are separated by 460 nucleotides
Pf6N2E2_3327 - Peptide chain release factor 3, at 3,847,838 to 3,849,427
_3327
Pf6N2E2_3328 - transcriptional regulator, at 3,849,669 to 3,849,905
_3328
Pf6N2E2_3329 - conserved hypothetical protein, at 3,850,003 to 3,850,359
_3329
Pf6N2E2_3330 - L-proline glycine betaine ABC transport system permease protein ProW (TC 3.A.1.12.1), at 3,850,820 to 3,851,536
_3330
Position (kb)
3849
3850 Strain fitness (log2 ratio)
-1
0
1 at 3848.850 kb on + strand, within Pf6N2E2_3327 at 3849.448 kb on + strand at 3849.449 kb on - strand at 3849.457 kb on - strand at 3849.457 kb on - strand at 3849.457 kb on - strand at 3849.457 kb on - strand at 3849.461 kb on - strand at 3849.461 kb on - strand at 3849.461 kb on - strand at 3849.461 kb on - strand at 3849.461 kb on - strand at 3849.461 kb on - strand at 3849.461 kb on - strand at 3849.521 kb on + strand at 3849.521 kb on + strand at 3849.521 kb on + strand at 3849.522 kb on - strand at 3849.623 kb on - strand at 3849.623 kb on - strand at 3849.623 kb on - strand at 3849.636 kb on + strand at 3849.636 kb on + strand at 3849.636 kb on + strand at 3849.637 kb on - strand at 3849.637 kb on - strand at 3849.639 kb on + strand at 3849.640 kb on - strand at 3849.658 kb on + strand at 3849.658 kb on + strand at 3849.659 kb on - strand at 3849.659 kb on - strand at 3849.659 kb on - strand at 3849.659 kb on - strand at 3849.659 kb on - strand at 3849.659 kb on - strand at 3849.670 kb on + strand at 3849.670 kb on + strand at 3849.670 kb on + strand at 3849.670 kb on + strand at 3849.670 kb on + strand at 3849.670 kb on + strand at 3849.670 kb on + strand at 3849.670 kb on + strand at 3849.671 kb on - strand at 3849.671 kb on - strand at 3849.671 kb on - strand at 3849.671 kb on - strand at 3849.671 kb on - strand at 3849.671 kb on - strand at 3849.761 kb on + strand, within Pf6N2E2_3328 at 3849.773 kb on - strand, within Pf6N2E2_3328 at 3849.773 kb on - strand, within Pf6N2E2_3328 at 3849.888 kb on + strand at 3850.060 kb on + strand, within Pf6N2E2_3329 at 3850.060 kb on + strand, within Pf6N2E2_3329 at 3850.060 kb on + strand, within Pf6N2E2_3329 at 3850.061 kb on - strand, within Pf6N2E2_3329 at 3850.069 kb on - strand, within Pf6N2E2_3329 at 3850.071 kb on - strand, within Pf6N2E2_3329 at 3850.071 kb on - strand, within Pf6N2E2_3329 at 3850.071 kb on - strand, within Pf6N2E2_3329 at 3850.194 kb on + strand, within Pf6N2E2_3329 at 3850.195 kb on - strand, within Pf6N2E2_3329 at 3850.368 kb on - strand at 3850.368 kb on - strand at 3850.368 kb on - strand at 3850.368 kb on - strand at 3850.397 kb on + strand at 3850.399 kb on + strand at 3850.530 kb on + strand at 3850.530 kb on + strand at 3850.530 kb on + strand at 3850.531 kb on - strand at 3850.634 kb on + strand at 3850.670 kb on + strand at 3850.702 kb on + strand at 3850.702 kb on + strand at 3850.703 kb on - strand at 3850.703 kb on - strand
Per-strain Table
Position Strand Gene LocusTag Fraction 50% P. fluorescens FW300-N2E2 spent media from growth in 20 mM putrescine remove 3,848,850 + Pf6N2E2_3327 0.64 -0.7 3,849,448 + +0.3 3,849,449 - +0.7 3,849,457 - +1.0 3,849,457 - -0.3 3,849,457 - +0.8 3,849,457 - -0.7 3,849,461 - -1.0 3,849,461 - +1.6 3,849,461 - -0.7 3,849,461 - -1.3 3,849,461 - +0.3 3,849,461 - +1.6 3,849,461 - -1.1 3,849,521 + +0.0 3,849,521 + -0.2 3,849,521 + +0.3 3,849,522 - +0.7 3,849,623 - +0.1 3,849,623 - +1.5 3,849,623 - +0.3 3,849,636 + +1.3 3,849,636 + +0.3 3,849,636 + +0.0 3,849,637 - -0.0 3,849,637 - +0.8 3,849,639 + -0.1 3,849,640 - +1.0 3,849,658 + +0.3 3,849,658 + +0.3 3,849,659 - +0.9 3,849,659 - +0.0 3,849,659 - +1.0 3,849,659 - +0.0 3,849,659 - +0.3 3,849,659 - +0.3 3,849,670 + -0.7 3,849,670 + +0.3 3,849,670 + +0.5 3,849,670 + +0.0 3,849,670 + +0.6 3,849,670 + -1.0 3,849,670 + +1.1 3,849,670 + +0.3 3,849,671 - -0.1 3,849,671 - +1.6 3,849,671 - +0.3 3,849,671 - +0.3 3,849,671 - -0.4 3,849,671 - +0.3 3,849,761 + Pf6N2E2_3328 0.39 -1.7 3,849,773 - Pf6N2E2_3328 0.44 -0.3 3,849,773 - Pf6N2E2_3328 0.44 +0.3 3,849,888 + +0.3 3,850,060 + Pf6N2E2_3329 0.16 -0.3 3,850,060 + Pf6N2E2_3329 0.16 -0.3 3,850,060 + Pf6N2E2_3329 0.16 -0.8 3,850,061 - Pf6N2E2_3329 0.16 +0.3 3,850,069 - Pf6N2E2_3329 0.18 +0.3 3,850,071 - Pf6N2E2_3329 0.19 +0.3 3,850,071 - Pf6N2E2_3329 0.19 +1.3 3,850,071 - Pf6N2E2_3329 0.19 +0.3 3,850,194 + Pf6N2E2_3329 0.54 +0.5 3,850,195 - Pf6N2E2_3329 0.54 +0.8 3,850,368 - -0.7 3,850,368 - +0.5 3,850,368 - +1.2 3,850,368 - -1.0 3,850,397 + +0.5 3,850,399 + -0.3 3,850,530 + -0.2 3,850,530 + +0.0 3,850,530 + +1.0 3,850,531 - +0.3 3,850,634 + +0.3 3,850,670 + +0.1 3,850,702 + -0.5 3,850,702 + +0.5 3,850,703 - +0.7 3,850,703 - -0.7
Or see this region's nucleotide sequence