Experiment: 50% P. fluorescens FW300-N2E2 spent media from growth in 20 mM putrescine
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt Pf6N2E2_3322 and Pf6N2E2_3323 are separated by 320 nucleotides Pf6N2E2_3323 and Pf6N2E2_3324 are separated by 53 nucleotides Pf6N2E2_3324 and Pf6N2E2_3325 are separated by 659 nucleotides
Pf6N2E2_3322 - Peptide synthetase, at 3,837,738 to 3,843,569
_3322
Pf6N2E2_3323 - hypothetical protein, at 3,843,890 to 3,844,618
_3323
Pf6N2E2_3324 - hypothetical protein, at 3,844,672 to 3,844,800
_3324
Pf6N2E2_3325 - ABC transporter, ATP-binding protein, at 3,845,460 to 3,846,485
_3325
Position (kb)
3843
3844
3845 Strain fitness (log2 ratio)
-3
-2
-1
0
1
2 at 3843.090 kb on + strand at 3843.091 kb on - strand at 3843.102 kb on - strand at 3843.412 kb on + strand at 3843.412 kb on + strand at 3843.413 kb on - strand at 3843.413 kb on - strand at 3843.422 kb on + strand at 3843.436 kb on - strand at 3843.465 kb on - strand at 3843.465 kb on - strand at 3843.465 kb on - strand at 3843.538 kb on - strand at 3843.569 kb on + strand at 3843.570 kb on - strand at 3843.571 kb on + strand at 3843.571 kb on + strand at 3843.572 kb on - strand at 3843.572 kb on - strand at 3843.572 kb on - strand at 3843.597 kb on - strand at 3843.597 kb on - strand at 3843.597 kb on - strand at 3843.597 kb on - strand at 3843.614 kb on - strand at 3843.614 kb on - strand at 3843.614 kb on - strand at 3843.625 kb on - strand at 3843.690 kb on - strand at 3843.828 kb on + strand at 3843.829 kb on - strand at 3843.848 kb on + strand at 3843.862 kb on + strand at 3843.862 kb on + strand at 3843.862 kb on + strand at 3843.863 kb on - strand at 3843.869 kb on + strand at 3843.909 kb on + strand at 3844.049 kb on + strand, within Pf6N2E2_3323 at 3844.049 kb on + strand, within Pf6N2E2_3323 at 3844.052 kb on + strand, within Pf6N2E2_3323 at 3844.052 kb on + strand, within Pf6N2E2_3323 at 3844.318 kb on + strand, within Pf6N2E2_3323 at 3844.380 kb on + strand, within Pf6N2E2_3323 at 3844.380 kb on + strand, within Pf6N2E2_3323 at 3845.014 kb on + strand at 3845.014 kb on + strand at 3845.042 kb on + strand at 3845.238 kb on + strand at 3845.386 kb on + strand
Per-strain Table
Position Strand Gene LocusTag Fraction 50% P. fluorescens FW300-N2E2 spent media from growth in 20 mM putrescine remove 3,843,090 + +1.4 3,843,091 - -0.3 3,843,102 - +0.7 3,843,412 + +0.6 3,843,412 + +0.3 3,843,413 - +0.9 3,843,413 - +0.1 3,843,422 + +0.6 3,843,436 - +1.1 3,843,465 - +0.8 3,843,465 - +0.1 3,843,465 - +1.5 3,843,538 - +0.3 3,843,569 + -1.0 3,843,570 - +2.3 3,843,571 + +1.0 3,843,571 + +0.5 3,843,572 - +0.3 3,843,572 - -1.1 3,843,572 - -1.1 3,843,597 - +0.3 3,843,597 - +0.3 3,843,597 - +0.8 3,843,597 - +2.6 3,843,614 - +0.0 3,843,614 - +0.1 3,843,614 - +1.8 3,843,625 - +0.3 3,843,690 - -0.7 3,843,828 + -0.3 3,843,829 - +0.3 3,843,848 + +0.3 3,843,862 + +1.0 3,843,862 + +0.4 3,843,862 + +0.3 3,843,863 - -1.0 3,843,869 + +0.3 3,843,909 + +0.3 3,844,049 + Pf6N2E2_3323 0.22 -1.2 3,844,049 + Pf6N2E2_3323 0.22 +0.3 3,844,052 + Pf6N2E2_3323 0.22 -2.3 3,844,052 + Pf6N2E2_3323 0.22 -0.7 3,844,318 + Pf6N2E2_3323 0.59 -2.2 3,844,380 + Pf6N2E2_3323 0.67 -1.9 3,844,380 + Pf6N2E2_3323 0.67 -2.9 3,845,014 + -2.1 3,845,014 + -1.2 3,845,042 + +1.3 3,845,238 + -2.1 3,845,386 + -2.9
Or see this region's nucleotide sequence