Experiment: 50% P. fluorescens FW300-N2E2 spent media from growth in 20 mM putrescine
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt Pf6N2E2_3201 and Pf6N2E2_3202 are separated by 150 nucleotides Pf6N2E2_3202 and Pf6N2E2_3203 are separated by 19 nucleotides
Pf6N2E2_3201 - Transcriptional regulator, MerR family, at 3,701,871 to 3,702,437
_3201
Pf6N2E2_3202 - Ribulose-5-phosphate 4-epimerase and related epimerases and aldolases, at 3,702,588 to 3,703,370
_3202
Pf6N2E2_3203 - 4-hydroxy-tetrahydrodipicolinate synthase (EC 4.3.3.7), at 3,703,390 to 3,704,277
_3203
Position (kb)
3701
3702
3703 Strain fitness (log2 ratio)
-2
-1
0
1
2 at 3700.871 kb on + strand at 3700.911 kb on + strand at 3701.178 kb on + strand at 3701.178 kb on + strand at 3701.178 kb on + strand at 3701.179 kb on - strand at 3701.231 kb on + strand at 3701.231 kb on + strand at 3701.231 kb on + strand at 3701.232 kb on - strand at 3701.232 kb on - strand at 3701.372 kb on + strand at 3701.373 kb on - strand at 3701.373 kb on - strand at 3701.373 kb on - strand at 3701.419 kb on + strand at 3701.420 kb on - strand at 3701.420 kb on - strand at 3701.429 kb on - strand at 3701.632 kb on + strand at 3701.632 kb on + strand at 3701.632 kb on + strand at 3701.633 kb on - strand at 3701.633 kb on - strand at 3701.633 kb on - strand at 3701.639 kb on - strand at 3701.639 kb on - strand at 3701.639 kb on - strand at 3701.741 kb on - strand at 3701.741 kb on - strand at 3702.229 kb on + strand, within Pf6N2E2_3201 at 3702.230 kb on - strand, within Pf6N2E2_3201 at 3702.230 kb on - strand, within Pf6N2E2_3201 at 3702.230 kb on - strand, within Pf6N2E2_3201 at 3702.230 kb on - strand, within Pf6N2E2_3201 at 3702.352 kb on + strand, within Pf6N2E2_3201 at 3702.353 kb on - strand, within Pf6N2E2_3201 at 3702.353 kb on - strand, within Pf6N2E2_3201 at 3702.437 kb on + strand at 3702.437 kb on + strand at 3702.437 kb on + strand at 3702.437 kb on + strand at 3702.438 kb on - strand at 3702.438 kb on - strand at 3702.438 kb on - strand at 3702.438 kb on - strand at 3702.438 kb on - strand at 3702.438 kb on - strand at 3702.438 kb on - strand at 3702.438 kb on - strand at 3702.438 kb on - strand at 3702.438 kb on - strand at 3702.438 kb on - strand at 3702.442 kb on - strand at 3702.587 kb on + strand at 3702.588 kb on - strand at 3702.668 kb on + strand, within Pf6N2E2_3202 at 3702.668 kb on + strand, within Pf6N2E2_3202 at 3702.668 kb on + strand, within Pf6N2E2_3202 at 3702.668 kb on + strand, within Pf6N2E2_3202 at 3702.668 kb on + strand, within Pf6N2E2_3202 at 3702.669 kb on - strand, within Pf6N2E2_3202 at 3702.669 kb on - strand, within Pf6N2E2_3202 at 3702.669 kb on - strand, within Pf6N2E2_3202 at 3702.669 kb on - strand, within Pf6N2E2_3202 at 3702.679 kb on + strand, within Pf6N2E2_3202 at 3702.680 kb on - strand, within Pf6N2E2_3202 at 3702.780 kb on + strand, within Pf6N2E2_3202 at 3702.780 kb on + strand, within Pf6N2E2_3202 at 3702.781 kb on - strand, within Pf6N2E2_3202 at 3702.781 kb on - strand, within Pf6N2E2_3202 at 3702.781 kb on - strand, within Pf6N2E2_3202 at 3702.781 kb on - strand, within Pf6N2E2_3202 at 3702.781 kb on - strand, within Pf6N2E2_3202 at 3702.783 kb on + strand, within Pf6N2E2_3202 at 3702.783 kb on + strand, within Pf6N2E2_3202 at 3702.784 kb on - strand, within Pf6N2E2_3202 at 3702.784 kb on - strand, within Pf6N2E2_3202 at 3702.784 kb on - strand, within Pf6N2E2_3202 at 3702.989 kb on - strand, within Pf6N2E2_3202 at 3703.027 kb on - strand, within Pf6N2E2_3202 at 3703.027 kb on - strand, within Pf6N2E2_3202 at 3703.027 kb on - strand, within Pf6N2E2_3202 at 3703.324 kb on - strand at 3703.324 kb on - strand at 3703.324 kb on - strand at 3703.324 kb on - strand at 3703.324 kb on - strand at 3703.326 kb on + strand at 3703.326 kb on + strand at 3703.327 kb on - strand at 3703.327 kb on - strand at 3703.327 kb on - strand at 3703.396 kb on - strand at 3703.423 kb on + strand at 3703.424 kb on - strand
Per-strain Table
Position Strand Gene LocusTag Fraction 50% P. fluorescens FW300-N2E2 spent media from growth in 20 mM putrescine remove 3,700,871 + -1.8 3,700,911 + -1.2 3,701,178 + -0.5 3,701,178 + +0.2 3,701,178 + -0.4 3,701,179 - +0.8 3,701,231 + -0.0 3,701,231 + -1.4 3,701,231 + +0.9 3,701,232 - +0.4 3,701,232 - +0.7 3,701,372 + -1.2 3,701,373 - +1.0 3,701,373 - -1.8 3,701,373 - +1.2 3,701,419 + +0.6 3,701,420 - +0.6 3,701,420 - +1.7 3,701,429 - +0.2 3,701,632 + +0.7 3,701,632 + -0.1 3,701,632 + +0.0 3,701,633 - +0.2 3,701,633 - +0.2 3,701,633 - +0.4 3,701,639 - +0.2 3,701,639 - +1.2 3,701,639 - -1.4 3,701,741 - -1.2 3,701,741 - -0.8 3,702,229 + Pf6N2E2_3201 0.63 +0.2 3,702,230 - Pf6N2E2_3201 0.63 -0.3 3,702,230 - Pf6N2E2_3201 0.63 -0.8 3,702,230 - Pf6N2E2_3201 0.63 -0.8 3,702,230 - Pf6N2E2_3201 0.63 -0.3 3,702,352 + Pf6N2E2_3201 0.85 -0.8 3,702,353 - Pf6N2E2_3201 0.85 -0.3 3,702,353 - Pf6N2E2_3201 0.85 -0.4 3,702,437 + +1.2 3,702,437 + -0.8 3,702,437 + -0.4 3,702,437 + -2.1 3,702,438 - -0.4 3,702,438 - +0.3 3,702,438 - +1.7 3,702,438 - +2.0 3,702,438 - +1.2 3,702,438 - -0.0 3,702,438 - -0.2 3,702,438 - +0.4 3,702,438 - +0.5 3,702,438 - -0.8 3,702,438 - +1.8 3,702,442 - -0.5 3,702,587 + +0.8 3,702,588 - +0.2 3,702,668 + Pf6N2E2_3202 0.10 +1.1 3,702,668 + Pf6N2E2_3202 0.10 -0.1 3,702,668 + Pf6N2E2_3202 0.10 +0.3 3,702,668 + Pf6N2E2_3202 0.10 -0.2 3,702,668 + Pf6N2E2_3202 0.10 -0.5 3,702,669 - Pf6N2E2_3202 0.10 +1.4 3,702,669 - Pf6N2E2_3202 0.10 +0.5 3,702,669 - Pf6N2E2_3202 0.10 +0.2 3,702,669 - Pf6N2E2_3202 0.10 +0.2 3,702,679 + Pf6N2E2_3202 0.12 -0.8 3,702,680 - Pf6N2E2_3202 0.12 +0.6 3,702,780 + Pf6N2E2_3202 0.25 +1.1 3,702,780 + Pf6N2E2_3202 0.25 -0.2 3,702,781 - Pf6N2E2_3202 0.25 -0.4 3,702,781 - Pf6N2E2_3202 0.25 -0.4 3,702,781 - Pf6N2E2_3202 0.25 +0.1 3,702,781 - Pf6N2E2_3202 0.25 -0.2 3,702,781 - Pf6N2E2_3202 0.25 +0.7 3,702,783 + Pf6N2E2_3202 0.25 +0.6 3,702,783 + Pf6N2E2_3202 0.25 -0.8 3,702,784 - Pf6N2E2_3202 0.25 -0.6 3,702,784 - Pf6N2E2_3202 0.25 +0.2 3,702,784 - Pf6N2E2_3202 0.25 +0.0 3,702,989 - Pf6N2E2_3202 0.51 +0.2 3,703,027 - Pf6N2E2_3202 0.56 -0.1 3,703,027 - Pf6N2E2_3202 0.56 +0.6 3,703,027 - Pf6N2E2_3202 0.56 +0.2 3,703,324 - +0.6 3,703,324 - -0.5 3,703,324 - +0.4 3,703,324 - +1.2 3,703,324 - -0.3 3,703,326 + +1.0 3,703,326 + +1.2 3,703,327 - +1.0 3,703,327 - +0.1 3,703,327 - -0.2 3,703,396 - +0.2 3,703,423 + +0.3 3,703,424 - -1.4
Or see this region's nucleotide sequence