Experiment: 50% P. fluorescens FW300-N2E2 spent media from growth in 20 mM putrescine
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt Pf6N2E2_3129 and Pf6N2E2_3130 are separated by 25 nucleotides Pf6N2E2_3130 and Pf6N2E2_3131 are separated by 72 nucleotides Pf6N2E2_3131 and Pf6N2E2_3132 are separated by 146 nucleotides Pf6N2E2_3132 and Pf6N2E2_3133 overlap by 38 nucleotides Pf6N2E2_3133 and Pf6N2E2_3134 are separated by 44 nucleotides
Pf6N2E2_3129 - Spermidine Putrescine ABC transporter permease component PotB (TC 3.A.1.11.1), at 3,620,673 to 3,621,620
_3129
Pf6N2E2_3130 - Spermidine Putrescine ABC transporter permease component potC (TC_3.A.1.11.1), at 3,621,646 to 3,622,428
_3130
Pf6N2E2_3131 - 4-aminobutyraldehyde dehydrogenase (EC 1.2.1.19), at 3,622,501 to 3,623,925
_3131
Pf6N2E2_3132 - FIG00954548: hypothetical protein, at 3,624,072 to 3,624,335
_3132
Pf6N2E2_3133 - Death on curing protein, Doc toxin, at 3,624,298 to 3,624,597
_3133
Pf6N2E2_3134 - hypothetical protein, at 3,624,642 to 3,624,827
_3134
Position (kb)
3622
3623
3624 Strain fitness (log2 ratio)
-2
-1
0
1
2 at 3621.576 kb on + strand at 3621.576 kb on + strand at 3621.576 kb on + strand at 3621.576 kb on + strand at 3621.576 kb on + strand at 3621.577 kb on - strand at 3621.577 kb on - strand at 3621.577 kb on - strand at 3621.580 kb on + strand at 3621.580 kb on + strand at 3621.580 kb on + strand at 3621.581 kb on - strand at 3621.581 kb on - strand at 3621.709 kb on + strand at 3621.919 kb on - strand, within Pf6N2E2_3130 at 3622.063 kb on + strand, within Pf6N2E2_3130 at 3622.157 kb on - strand, within Pf6N2E2_3130 at 3622.349 kb on + strand, within Pf6N2E2_3130 at 3622.349 kb on + strand, within Pf6N2E2_3130 at 3622.350 kb on - strand at 3622.378 kb on + strand at 3622.378 kb on + strand at 3622.379 kb on - strand at 3622.379 kb on - strand at 3622.381 kb on + strand at 3622.381 kb on + strand at 3622.381 kb on + strand at 3622.426 kb on + strand at 3622.426 kb on + strand at 3622.426 kb on + strand at 3622.427 kb on - strand at 3622.427 kb on - strand at 3622.876 kb on + strand, within Pf6N2E2_3131 at 3622.948 kb on + strand, within Pf6N2E2_3131 at 3622.948 kb on + strand, within Pf6N2E2_3131 at 3622.949 kb on - strand, within Pf6N2E2_3131 at 3623.001 kb on + strand, within Pf6N2E2_3131 at 3623.087 kb on + strand, within Pf6N2E2_3131 at 3623.088 kb on - strand, within Pf6N2E2_3131 at 3623.088 kb on - strand, within Pf6N2E2_3131 at 3623.320 kb on + strand, within Pf6N2E2_3131 at 3623.321 kb on - strand, within Pf6N2E2_3131 at 3623.359 kb on + strand, within Pf6N2E2_3131 at 3623.360 kb on - strand, within Pf6N2E2_3131 at 3623.360 kb on - strand, within Pf6N2E2_3131 at 3623.360 kb on - strand, within Pf6N2E2_3131 at 3623.377 kb on + strand, within Pf6N2E2_3131 at 3623.377 kb on + strand, within Pf6N2E2_3131 at 3623.378 kb on - strand, within Pf6N2E2_3131 at 3623.378 kb on - strand, within Pf6N2E2_3131 at 3623.425 kb on + strand, within Pf6N2E2_3131 at 3623.425 kb on + strand, within Pf6N2E2_3131 at 3623.425 kb on + strand, within Pf6N2E2_3131 at 3623.426 kb on - strand, within Pf6N2E2_3131 at 3623.529 kb on + strand, within Pf6N2E2_3131 at 3623.529 kb on + strand, within Pf6N2E2_3131 at 3623.530 kb on - strand, within Pf6N2E2_3131 at 3623.530 kb on - strand, within Pf6N2E2_3131 at 3623.530 kb on - strand, within Pf6N2E2_3131 at 3623.530 kb on - strand, within Pf6N2E2_3131 at 3623.684 kb on + strand, within Pf6N2E2_3131 at 3623.684 kb on + strand, within Pf6N2E2_3131 at 3623.684 kb on + strand, within Pf6N2E2_3131 at 3623.685 kb on - strand, within Pf6N2E2_3131 at 3623.685 kb on - strand, within Pf6N2E2_3131 at 3623.685 kb on - strand, within Pf6N2E2_3131 at 3623.779 kb on + strand, within Pf6N2E2_3131 at 3623.779 kb on + strand, within Pf6N2E2_3131 at 3623.780 kb on - strand, within Pf6N2E2_3131 at 3623.780 kb on - strand, within Pf6N2E2_3131 at 3623.875 kb on + strand at 3623.875 kb on + strand at 3623.875 kb on + strand at 3623.875 kb on + strand at 3623.875 kb on + strand at 3623.876 kb on - strand at 3623.876 kb on - strand at 3623.876 kb on - strand at 3623.876 kb on - strand at 3623.891 kb on - strand at 3623.980 kb on - strand at 3623.980 kb on - strand at 3623.980 kb on - strand at 3624.044 kb on + strand at 3624.051 kb on + strand at 3624.052 kb on - strand at 3624.052 kb on - strand at 3624.052 kb on - strand at 3624.052 kb on - strand at 3624.052 kb on - strand at 3624.053 kb on + strand at 3624.053 kb on + strand at 3624.054 kb on - strand at 3624.054 kb on - strand at 3624.163 kb on - strand, within Pf6N2E2_3132 at 3624.163 kb on - strand, within Pf6N2E2_3132 at 3624.190 kb on - strand, within Pf6N2E2_3132 at 3624.190 kb on - strand, within Pf6N2E2_3132 at 3624.190 kb on - strand, within Pf6N2E2_3132 at 3624.284 kb on + strand, within Pf6N2E2_3132 at 3624.284 kb on + strand, within Pf6N2E2_3132 at 3624.284 kb on + strand, within Pf6N2E2_3132 at 3624.311 kb on + strand at 3624.311 kb on + strand at 3624.312 kb on - strand at 3624.680 kb on - strand, within Pf6N2E2_3134 at 3624.816 kb on + strand at 3624.842 kb on + strand at 3624.842 kb on + strand at 3624.842 kb on + strand at 3624.907 kb on - strand
Per-strain Table
Position Strand Gene LocusTag Fraction 50% P. fluorescens FW300-N2E2 spent media from growth in 20 mM putrescine remove 3,621,576 + +0.6 3,621,576 + +0.9 3,621,576 + +0.5 3,621,576 + -0.0 3,621,576 + +0.7 3,621,577 - -0.0 3,621,577 - +0.8 3,621,577 - +0.9 3,621,580 + +1.8 3,621,580 + +0.2 3,621,580 + +1.8 3,621,581 - +1.0 3,621,581 - +0.1 3,621,709 + +0.4 3,621,919 - Pf6N2E2_3130 0.35 -2.1 3,622,063 + Pf6N2E2_3130 0.53 +0.8 3,622,157 - Pf6N2E2_3130 0.65 -0.0 3,622,349 + Pf6N2E2_3130 0.90 -0.9 3,622,349 + Pf6N2E2_3130 0.90 +0.6 3,622,350 - +0.2 3,622,378 + +0.6 3,622,378 + -0.1 3,622,379 - +0.2 3,622,379 - -0.2 3,622,381 + -0.4 3,622,381 + +0.3 3,622,381 + -0.4 3,622,426 + +1.8 3,622,426 + +0.7 3,622,426 + +0.2 3,622,427 - +0.2 3,622,427 - +0.6 3,622,876 + Pf6N2E2_3131 0.26 +0.2 3,622,948 + Pf6N2E2_3131 0.31 -1.8 3,622,948 + Pf6N2E2_3131 0.31 -0.8 3,622,949 - Pf6N2E2_3131 0.31 +0.8 3,623,001 + Pf6N2E2_3131 0.35 +0.2 3,623,087 + Pf6N2E2_3131 0.41 -1.0 3,623,088 - Pf6N2E2_3131 0.41 +0.1 3,623,088 - Pf6N2E2_3131 0.41 +0.5 3,623,320 + Pf6N2E2_3131 0.57 -0.2 3,623,321 - Pf6N2E2_3131 0.58 +0.2 3,623,359 + Pf6N2E2_3131 0.60 -0.2 3,623,360 - Pf6N2E2_3131 0.60 -0.0 3,623,360 - Pf6N2E2_3131 0.60 +1.5 3,623,360 - Pf6N2E2_3131 0.60 -0.8 3,623,377 + Pf6N2E2_3131 0.61 +0.5 3,623,377 + Pf6N2E2_3131 0.61 -0.4 3,623,378 - Pf6N2E2_3131 0.62 +0.6 3,623,378 - Pf6N2E2_3131 0.62 -0.5 3,623,425 + Pf6N2E2_3131 0.65 -1.0 3,623,425 + Pf6N2E2_3131 0.65 +0.8 3,623,425 + Pf6N2E2_3131 0.65 -0.2 3,623,426 - Pf6N2E2_3131 0.65 +1.1 3,623,529 + Pf6N2E2_3131 0.72 +0.2 3,623,529 + Pf6N2E2_3131 0.72 +0.6 3,623,530 - Pf6N2E2_3131 0.72 +0.5 3,623,530 - Pf6N2E2_3131 0.72 +1.2 3,623,530 - Pf6N2E2_3131 0.72 -1.8 3,623,530 - Pf6N2E2_3131 0.72 +0.5 3,623,684 + Pf6N2E2_3131 0.83 -0.8 3,623,684 + Pf6N2E2_3131 0.83 +0.4 3,623,684 + Pf6N2E2_3131 0.83 +0.8 3,623,685 - Pf6N2E2_3131 0.83 +0.6 3,623,685 - Pf6N2E2_3131 0.83 +0.2 3,623,685 - Pf6N2E2_3131 0.83 +0.2 3,623,779 + Pf6N2E2_3131 0.90 +0.4 3,623,779 + Pf6N2E2_3131 0.90 +1.0 3,623,780 - Pf6N2E2_3131 0.90 -0.0 3,623,780 - Pf6N2E2_3131 0.90 +2.1 3,623,875 + -0.2 3,623,875 + +0.8 3,623,875 + -0.6 3,623,875 + -0.6 3,623,875 + +0.2 3,623,876 - -0.8 3,623,876 - +0.8 3,623,876 - -0.3 3,623,876 - -0.5 3,623,891 - +0.1 3,623,980 - +0.1 3,623,980 - -0.8 3,623,980 - +0.2 3,624,044 + -0.2 3,624,051 + +1.4 3,624,052 - +2.3 3,624,052 - +0.7 3,624,052 - -1.8 3,624,052 - +0.2 3,624,052 - +0.6 3,624,053 + +1.1 3,624,053 + +0.5 3,624,054 - +0.5 3,624,054 - +0.8 3,624,163 - Pf6N2E2_3132 0.34 +0.2 3,624,163 - Pf6N2E2_3132 0.34 -0.8 3,624,190 - Pf6N2E2_3132 0.45 +1.2 3,624,190 - Pf6N2E2_3132 0.45 +0.2 3,624,190 - Pf6N2E2_3132 0.45 +0.2 3,624,284 + Pf6N2E2_3132 0.80 -1.0 3,624,284 + Pf6N2E2_3132 0.80 +0.2 3,624,284 + Pf6N2E2_3132 0.80 -0.8 3,624,311 + +1.6 3,624,311 + +0.2 3,624,312 - +0.9 3,624,680 - Pf6N2E2_3134 0.20 +0.2 3,624,816 + +0.2 3,624,842 + -0.8 3,624,842 + +0.2 3,624,842 + +0.2 3,624,907 - -0.8
Or see this region's nucleotide sequence