Experiment: 50% P. fluorescens FW300-N2E2 spent media from growth in 20 mM putrescine
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt Pf6N2E2_3083 and Pf6N2E2_3084 overlap by 1 nucleotides Pf6N2E2_3084 and Pf6N2E2_3085 are separated by 82 nucleotides Pf6N2E2_3085 and Pf6N2E2_3086 are separated by 147 nucleotides Pf6N2E2_3086 and Pf6N2E2_3087 are separated by 234 nucleotides
Pf6N2E2_3083 - N-succinyl-L,L-diaminopimelate desuccinylase (EC 3.5.1.18), at 3,570,179 to 3,571,330
_3083
Pf6N2E2_3084 - Ribosomal RNA large subunit methyltransferase A (EC 2.1.1.51), at 3,571,330 to 3,572,139
_3084
Pf6N2E2_3085 - FIG00954614: hypothetical protein, at 3,572,222 to 3,572,599
_3085
Pf6N2E2_3086 - Cold shock protein CspA, at 3,572,747 to 3,572,956
_3086
Pf6N2E2_3087 - Glycerol-3-phosphate acyltransferase (EC 2.3.1.15), at 3,573,191 to 3,575,689
_3087
Position (kb)
3572
3573 Strain fitness (log2 ratio)
-2
-1
0
1
2 at 3571.525 kb on + strand, within Pf6N2E2_3084 at 3571.609 kb on + strand, within Pf6N2E2_3084 at 3571.609 kb on + strand, within Pf6N2E2_3084 at 3571.609 kb on + strand, within Pf6N2E2_3084 at 3571.610 kb on - strand, within Pf6N2E2_3084 at 3571.610 kb on - strand, within Pf6N2E2_3084 at 3571.612 kb on + strand, within Pf6N2E2_3084 at 3571.612 kb on + strand, within Pf6N2E2_3084 at 3571.613 kb on - strand, within Pf6N2E2_3084 at 3571.613 kb on - strand, within Pf6N2E2_3084 at 3571.613 kb on - strand, within Pf6N2E2_3084 at 3571.909 kb on + strand, within Pf6N2E2_3084 at 3571.909 kb on + strand, within Pf6N2E2_3084 at 3571.909 kb on + strand, within Pf6N2E2_3084 at 3571.910 kb on - strand, within Pf6N2E2_3084 at 3571.910 kb on - strand, within Pf6N2E2_3084 at 3572.117 kb on - strand at 3572.122 kb on + strand at 3572.122 kb on + strand at 3572.123 kb on - strand at 3572.247 kb on - strand at 3572.343 kb on - strand, within Pf6N2E2_3085 at 3572.343 kb on - strand, within Pf6N2E2_3085 at 3572.582 kb on - strand at 3572.582 kb on - strand at 3572.659 kb on - strand at 3572.659 kb on - strand at 3572.673 kb on - strand at 3572.731 kb on - strand at 3572.748 kb on + strand at 3572.749 kb on - strand at 3572.976 kb on - strand at 3572.976 kb on - strand at 3572.980 kb on + strand at 3572.980 kb on + strand at 3572.981 kb on - strand at 3572.981 kb on - strand at 3572.982 kb on + strand at 3572.982 kb on + strand at 3572.982 kb on + strand at 3572.982 kb on + strand at 3572.983 kb on - strand at 3572.983 kb on - strand at 3572.983 kb on - strand at 3572.983 kb on - strand at 3573.009 kb on - strand at 3573.066 kb on + strand at 3573.066 kb on + strand at 3573.066 kb on + strand at 3573.066 kb on + strand at 3573.067 kb on - strand at 3573.067 kb on - strand at 3573.067 kb on - strand at 3573.067 kb on - strand at 3573.068 kb on + strand at 3573.068 kb on + strand at 3573.068 kb on + strand at 3573.069 kb on - strand at 3573.069 kb on - strand at 3573.069 kb on - strand at 3573.069 kb on - strand at 3573.069 kb on - strand at 3573.100 kb on - strand at 3573.142 kb on + strand at 3573.169 kb on + strand at 3573.190 kb on + strand at 3573.191 kb on - strand at 3573.245 kb on + strand at 3573.245 kb on + strand at 3573.246 kb on - strand at 3573.246 kb on - strand at 3573.246 kb on - strand at 3573.246 kb on - strand at 3573.320 kb on + strand at 3573.320 kb on + strand at 3573.320 kb on + strand at 3573.320 kb on + strand at 3573.321 kb on - strand at 3573.321 kb on - strand at 3573.321 kb on - strand at 3573.321 kb on - strand at 3573.321 kb on - strand at 3573.321 kb on - strand at 3573.321 kb on - strand
Per-strain Table
Position Strand Gene LocusTag Fraction 50% P. fluorescens FW300-N2E2 spent media from growth in 20 mM putrescine remove 3,571,525 + Pf6N2E2_3084 0.24 +0.3 3,571,609 + Pf6N2E2_3084 0.34 +0.6 3,571,609 + Pf6N2E2_3084 0.34 -0.4 3,571,609 + Pf6N2E2_3084 0.34 +1.1 3,571,610 - Pf6N2E2_3084 0.35 +0.8 3,571,610 - Pf6N2E2_3084 0.35 -1.4 3,571,612 + Pf6N2E2_3084 0.35 +1.0 3,571,612 + Pf6N2E2_3084 0.35 +1.5 3,571,613 - Pf6N2E2_3084 0.35 -1.2 3,571,613 - Pf6N2E2_3084 0.35 +1.4 3,571,613 - Pf6N2E2_3084 0.35 -0.3 3,571,909 + Pf6N2E2_3084 0.71 +0.8 3,571,909 + Pf6N2E2_3084 0.71 -0.3 3,571,909 + Pf6N2E2_3084 0.71 -0.8 3,571,910 - Pf6N2E2_3084 0.72 +0.5 3,571,910 - Pf6N2E2_3084 0.72 +0.1 3,572,117 - +0.2 3,572,122 + +0.3 3,572,122 + +0.0 3,572,123 - +1.2 3,572,247 - -0.7 3,572,343 - Pf6N2E2_3085 0.32 -1.8 3,572,343 - Pf6N2E2_3085 0.32 -1.0 3,572,582 - +1.2 3,572,582 - +0.2 3,572,659 - -2.1 3,572,659 - +0.2 3,572,673 - -0.2 3,572,731 - +0.7 3,572,748 + +0.5 3,572,749 - +0.4 3,572,976 - +0.8 3,572,976 - -0.8 3,572,980 + +0.5 3,572,980 + +1.2 3,572,981 - -1.1 3,572,981 - -1.1 3,572,982 + -1.4 3,572,982 + +2.2 3,572,982 + -2.1 3,572,982 + +0.2 3,572,983 - +0.1 3,572,983 - -0.8 3,572,983 - +0.2 3,572,983 - +0.2 3,573,009 - +0.2 3,573,066 + +0.8 3,573,066 + +0.9 3,573,066 + -0.2 3,573,066 + +0.6 3,573,067 - -2.1 3,573,067 - +0.2 3,573,067 - +0.2 3,573,067 - -0.3 3,573,068 + +1.8 3,573,068 + -1.8 3,573,068 + -1.0 3,573,069 - -0.4 3,573,069 - -0.4 3,573,069 - -0.4 3,573,069 - -0.5 3,573,069 - +1.2 3,573,100 - +0.2 3,573,142 + -0.2 3,573,169 + +0.5 3,573,190 + +1.1 3,573,191 - +1.5 3,573,245 + +0.7 3,573,245 + +1.3 3,573,246 - +0.8 3,573,246 - -0.3 3,573,246 - -0.2 3,573,246 - +0.1 3,573,320 + +1.1 3,573,320 + +0.5 3,573,320 + +1.3 3,573,320 + +0.2 3,573,321 - -0.8 3,573,321 - +1.2 3,573,321 - +0.2 3,573,321 - +0.7 3,573,321 - +0.3 3,573,321 - +0.4 3,573,321 - +0.4
Or see this region's nucleotide sequence