Experiment: 50% P. fluorescens FW300-N2E2 spent media from growth in 20 mM putrescine
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt Pf6N2E2_2978 and Pf6N2E2_2979 are separated by 263 nucleotides Pf6N2E2_2979 and Pf6N2E2_2980 are separated by 152 nucleotides Pf6N2E2_2980 and Pf6N2E2_2981 are separated by 57 nucleotides
Pf6N2E2_2978 - Ribosomal protein S12p Asp88 (E. coli) methylthiotransferase, at 3,473,781 to 3,475,121
_2978
Pf6N2E2_2979 - Histone acetyltransferase HPA2 and related acetyltransferases, at 3,475,385 to 3,475,858
_2979
Pf6N2E2_2980 - tRNA pseudouridine synthase A (EC 4.2.1.70), at 3,476,011 to 3,476,652
_2980
Pf6N2E2_2981 - FIG00956655: hypothetical protein, at 3,476,710 to 3,477,171
_2981
Position (kb)
3475
3476 Strain fitness (log2 ratio)
-1
0
1
2
3
4
5 at 3474.385 kb on - strand, within Pf6N2E2_2978 at 3474.403 kb on - strand, within Pf6N2E2_2978 at 3474.498 kb on + strand, within Pf6N2E2_2978 at 3474.498 kb on + strand, within Pf6N2E2_2978 at 3474.498 kb on + strand, within Pf6N2E2_2978 at 3474.498 kb on + strand, within Pf6N2E2_2978 at 3474.498 kb on + strand, within Pf6N2E2_2978 at 3474.498 kb on + strand, within Pf6N2E2_2978 at 3474.499 kb on - strand, within Pf6N2E2_2978 at 3474.499 kb on - strand, within Pf6N2E2_2978 at 3474.499 kb on - strand, within Pf6N2E2_2978 at 3474.499 kb on - strand, within Pf6N2E2_2978 at 3474.499 kb on - strand, within Pf6N2E2_2978 at 3474.499 kb on - strand, within Pf6N2E2_2978 at 3474.499 kb on - strand, within Pf6N2E2_2978 at 3474.499 kb on - strand, within Pf6N2E2_2978 at 3474.504 kb on + strand, within Pf6N2E2_2978 at 3474.742 kb on - strand, within Pf6N2E2_2978 at 3474.795 kb on + strand, within Pf6N2E2_2978 at 3474.795 kb on + strand, within Pf6N2E2_2978 at 3475.344 kb on - strand at 3475.461 kb on + strand, within Pf6N2E2_2979 at 3475.493 kb on + strand, within Pf6N2E2_2979 at 3475.494 kb on - strand, within Pf6N2E2_2979 at 3475.494 kb on - strand, within Pf6N2E2_2979 at 3475.494 kb on - strand, within Pf6N2E2_2979 at 3475.494 kb on - strand, within Pf6N2E2_2979 at 3475.494 kb on - strand, within Pf6N2E2_2979 at 3475.494 kb on - strand, within Pf6N2E2_2979 at 3475.494 kb on - strand, within Pf6N2E2_2979 at 3475.529 kb on + strand, within Pf6N2E2_2979 at 3475.674 kb on - strand, within Pf6N2E2_2979 at 3475.674 kb on - strand, within Pf6N2E2_2979 at 3475.674 kb on - strand, within Pf6N2E2_2979 at 3475.742 kb on + strand, within Pf6N2E2_2979 at 3475.742 kb on + strand, within Pf6N2E2_2979 at 3475.743 kb on - strand, within Pf6N2E2_2979 at 3475.743 kb on - strand, within Pf6N2E2_2979 at 3475.824 kb on - strand at 3475.907 kb on - strand at 3475.907 kb on - strand at 3475.907 kb on - strand at 3475.907 kb on - strand at 3475.907 kb on - strand at 3475.907 kb on - strand at 3476.168 kb on + strand, within Pf6N2E2_2980 at 3476.168 kb on + strand, within Pf6N2E2_2980 at 3476.168 kb on + strand, within Pf6N2E2_2980 at 3476.168 kb on + strand, within Pf6N2E2_2980 at 3476.169 kb on - strand, within Pf6N2E2_2980 at 3476.169 kb on - strand, within Pf6N2E2_2980 at 3476.169 kb on - strand, within Pf6N2E2_2980 at 3476.169 kb on - strand, within Pf6N2E2_2980 at 3476.169 kb on - strand, within Pf6N2E2_2980 at 3476.169 kb on - strand, within Pf6N2E2_2980 at 3476.169 kb on - strand, within Pf6N2E2_2980 at 3476.368 kb on + strand, within Pf6N2E2_2980 at 3476.368 kb on + strand, within Pf6N2E2_2980 at 3476.368 kb on + strand, within Pf6N2E2_2980 at 3476.368 kb on + strand, within Pf6N2E2_2980 at 3476.368 kb on + strand, within Pf6N2E2_2980 at 3476.368 kb on + strand, within Pf6N2E2_2980 at 3476.369 kb on - strand, within Pf6N2E2_2980 at 3476.369 kb on - strand, within Pf6N2E2_2980 at 3476.369 kb on - strand, within Pf6N2E2_2980 at 3476.369 kb on - strand, within Pf6N2E2_2980 at 3476.369 kb on - strand, within Pf6N2E2_2980 at 3476.369 kb on - strand, within Pf6N2E2_2980 at 3476.369 kb on - strand, within Pf6N2E2_2980 at 3476.369 kb on - strand, within Pf6N2E2_2980 at 3476.369 kb on - strand, within Pf6N2E2_2980 at 3476.369 kb on - strand, within Pf6N2E2_2980 at 3476.434 kb on + strand, within Pf6N2E2_2980 at 3476.434 kb on + strand, within Pf6N2E2_2980 at 3476.434 kb on + strand, within Pf6N2E2_2980 at 3476.435 kb on - strand, within Pf6N2E2_2980 at 3476.435 kb on - strand, within Pf6N2E2_2980 at 3476.435 kb on - strand, within Pf6N2E2_2980 at 3476.435 kb on - strand, within Pf6N2E2_2980 at 3476.435 kb on - strand, within Pf6N2E2_2980 at 3476.435 kb on - strand, within Pf6N2E2_2980 at 3476.435 kb on - strand, within Pf6N2E2_2980 at 3476.626 kb on + strand at 3476.627 kb on - strand at 3476.651 kb on - strand at 3476.726 kb on + strand at 3476.726 kb on + strand at 3476.727 kb on - strand at 3476.743 kb on + strand at 3476.743 kb on + strand at 3476.743 kb on + strand at 3476.743 kb on + strand at 3476.743 kb on + strand at 3476.744 kb on - strand at 3476.744 kb on - strand at 3476.828 kb on - strand, within Pf6N2E2_2981 at 3476.828 kb on - strand, within Pf6N2E2_2981 at 3476.828 kb on - strand, within Pf6N2E2_2981
Per-strain Table
Position Strand Gene LocusTag Fraction 50% P. fluorescens FW300-N2E2 spent media from growth in 20 mM putrescine remove 3,474,385 - Pf6N2E2_2978 0.45 -0.9 3,474,403 - Pf6N2E2_2978 0.46 -0.6 3,474,498 + Pf6N2E2_2978 0.53 +0.1 3,474,498 + Pf6N2E2_2978 0.53 +1.1 3,474,498 + Pf6N2E2_2978 0.53 +0.1 3,474,498 + Pf6N2E2_2978 0.53 +0.1 3,474,498 + Pf6N2E2_2978 0.53 -0.6 3,474,498 + Pf6N2E2_2978 0.53 +0.1 3,474,499 - Pf6N2E2_2978 0.54 -0.3 3,474,499 - Pf6N2E2_2978 0.54 +2.5 3,474,499 - Pf6N2E2_2978 0.54 -0.7 3,474,499 - Pf6N2E2_2978 0.54 +0.5 3,474,499 - Pf6N2E2_2978 0.54 -0.9 3,474,499 - Pf6N2E2_2978 0.54 -0.9 3,474,499 - Pf6N2E2_2978 0.54 -1.4 3,474,499 - Pf6N2E2_2978 0.54 -0.4 3,474,504 + Pf6N2E2_2978 0.54 +0.1 3,474,742 - Pf6N2E2_2978 0.72 -1.4 3,474,795 + Pf6N2E2_2978 0.76 +0.7 3,474,795 + Pf6N2E2_2978 0.76 +0.2 3,475,344 - +2.9 3,475,461 + Pf6N2E2_2979 0.16 +3.1 3,475,493 + Pf6N2E2_2979 0.23 +1.1 3,475,494 - Pf6N2E2_2979 0.23 +2.0 3,475,494 - Pf6N2E2_2979 0.23 +1.0 3,475,494 - Pf6N2E2_2979 0.23 +3.3 3,475,494 - Pf6N2E2_2979 0.23 +2.1 3,475,494 - Pf6N2E2_2979 0.23 +2.2 3,475,494 - Pf6N2E2_2979 0.23 +2.3 3,475,494 - Pf6N2E2_2979 0.23 +2.0 3,475,529 + Pf6N2E2_2979 0.30 +3.2 3,475,674 - Pf6N2E2_2979 0.61 +1.8 3,475,674 - Pf6N2E2_2979 0.61 +2.3 3,475,674 - Pf6N2E2_2979 0.61 +3.5 3,475,742 + Pf6N2E2_2979 0.75 +5.1 3,475,742 + Pf6N2E2_2979 0.75 +3.8 3,475,743 - Pf6N2E2_2979 0.76 +1.7 3,475,743 - Pf6N2E2_2979 0.76 +1.7 3,475,824 - +1.1 3,475,907 - +0.1 3,475,907 - -0.4 3,475,907 - +0.3 3,475,907 - +0.3 3,475,907 - -0.9 3,475,907 - -0.4 3,476,168 + Pf6N2E2_2980 0.24 +3.1 3,476,168 + Pf6N2E2_2980 0.24 +1.1 3,476,168 + Pf6N2E2_2980 0.24 +1.5 3,476,168 + Pf6N2E2_2980 0.24 -0.9 3,476,169 - Pf6N2E2_2980 0.25 -0.9 3,476,169 - Pf6N2E2_2980 0.25 +0.1 3,476,169 - Pf6N2E2_2980 0.25 -0.7 3,476,169 - Pf6N2E2_2980 0.25 +0.4 3,476,169 - Pf6N2E2_2980 0.25 +0.1 3,476,169 - Pf6N2E2_2980 0.25 -0.0 3,476,169 - Pf6N2E2_2980 0.25 +2.5 3,476,368 + Pf6N2E2_2980 0.56 +0.1 3,476,368 + Pf6N2E2_2980 0.56 +1.4 3,476,368 + Pf6N2E2_2980 0.56 +1.1 3,476,368 + Pf6N2E2_2980 0.56 +0.1 3,476,368 + Pf6N2E2_2980 0.56 +1.1 3,476,368 + Pf6N2E2_2980 0.56 -0.9 3,476,369 - Pf6N2E2_2980 0.56 -0.9 3,476,369 - Pf6N2E2_2980 0.56 +0.8 3,476,369 - Pf6N2E2_2980 0.56 +0.0 3,476,369 - Pf6N2E2_2980 0.56 -0.3 3,476,369 - Pf6N2E2_2980 0.56 +0.1 3,476,369 - Pf6N2E2_2980 0.56 -0.7 3,476,369 - Pf6N2E2_2980 0.56 -1.5 3,476,369 - Pf6N2E2_2980 0.56 -1.1 3,476,369 - Pf6N2E2_2980 0.56 +0.4 3,476,369 - Pf6N2E2_2980 0.56 -0.9 3,476,434 + Pf6N2E2_2980 0.66 -0.3 3,476,434 + Pf6N2E2_2980 0.66 +0.1 3,476,434 + Pf6N2E2_2980 0.66 +0.4 3,476,435 - Pf6N2E2_2980 0.66 +0.8 3,476,435 - Pf6N2E2_2980 0.66 -0.6 3,476,435 - Pf6N2E2_2980 0.66 -0.5 3,476,435 - Pf6N2E2_2980 0.66 -1.7 3,476,435 - Pf6N2E2_2980 0.66 -0.9 3,476,435 - Pf6N2E2_2980 0.66 +0.4 3,476,435 - Pf6N2E2_2980 0.66 +0.1 3,476,626 + +1.0 3,476,627 - -0.7 3,476,651 - +0.5 3,476,726 + -1.6 3,476,726 + -0.1 3,476,727 - -0.4 3,476,743 + -0.0 3,476,743 + -0.3 3,476,743 + +0.6 3,476,743 + -0.6 3,476,743 + -1.4 3,476,744 - +1.1 3,476,744 - +0.1 3,476,828 - Pf6N2E2_2981 0.26 +0.1 3,476,828 - Pf6N2E2_2981 0.26 -0.4 3,476,828 - Pf6N2E2_2981 0.26 -0.4
Or see this region's nucleotide sequence