Experiment: 50% P. fluorescens FW300-N2E2 spent media from growth in 20 mM putrescine
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt Pf6N2E2_259 and Pf6N2E2_260 are separated by 56 nucleotides Pf6N2E2_260 and Pf6N2E2_261 are separated by 198 nucleotides Pf6N2E2_261 and Pf6N2E2_262 are separated by 70 nucleotides Pf6N2E2_262 and Pf6N2E2_263 are separated by 263 nucleotides
Pf6N2E2_259 - Transcriptional regulator, LysR family, at 323,206 to 324,084
_259
Pf6N2E2_260 - Regulator of cell morphogenesis and NO signaling, at 324,141 to 324,617
_260
Pf6N2E2_261 - Aminobutyraldehyde dehydrogenase (EC 1.2.1.19), at 324,816 to 325,925
_261
Pf6N2E2_262 - hypothetical protein, at 325,996 to 326,292
_262
Pf6N2E2_263 - Transcriptional regulator containing an amidase domain and an AraC-type DNA-binding HTH domain, at 326,556 to 327,200
_263
Position (kb)
324
325
326 Strain fitness (log2 ratio)
-2
-1
0
1
2 at 323.914 kb on + strand, within Pf6N2E2_259 at 323.914 kb on + strand, within Pf6N2E2_259 at 323.914 kb on + strand, within Pf6N2E2_259 at 323.915 kb on - strand, within Pf6N2E2_259 at 323.915 kb on - strand, within Pf6N2E2_259 at 323.915 kb on - strand, within Pf6N2E2_259 at 323.915 kb on - strand, within Pf6N2E2_259 at 323.915 kb on - strand, within Pf6N2E2_259 at 324.083 kb on - strand at 324.083 kb on - strand at 324.128 kb on + strand at 324.128 kb on + strand at 324.129 kb on - strand at 324.179 kb on - strand at 324.383 kb on + strand, within Pf6N2E2_260 at 324.406 kb on + strand, within Pf6N2E2_260 at 324.406 kb on + strand, within Pf6N2E2_260 at 324.407 kb on - strand, within Pf6N2E2_260 at 324.409 kb on + strand, within Pf6N2E2_260 at 324.409 kb on + strand, within Pf6N2E2_260 at 324.410 kb on - strand, within Pf6N2E2_260 at 324.617 kb on + strand at 324.617 kb on + strand at 324.617 kb on + strand at 324.617 kb on + strand at 324.617 kb on + strand at 324.617 kb on + strand at 324.617 kb on + strand at 324.618 kb on - strand at 324.618 kb on - strand at 324.618 kb on - strand at 324.618 kb on - strand at 324.621 kb on + strand at 324.621 kb on + strand at 324.621 kb on + strand at 324.621 kb on + strand at 324.621 kb on + strand at 324.635 kb on + strand at 325.034 kb on - strand, within Pf6N2E2_261 at 325.228 kb on + strand, within Pf6N2E2_261 at 325.228 kb on + strand, within Pf6N2E2_261 at 325.229 kb on - strand, within Pf6N2E2_261 at 325.408 kb on - strand, within Pf6N2E2_261 at 325.507 kb on + strand, within Pf6N2E2_261 at 325.507 kb on + strand, within Pf6N2E2_261 at 325.508 kb on - strand, within Pf6N2E2_261 at 325.609 kb on - strand, within Pf6N2E2_261 at 325.609 kb on - strand, within Pf6N2E2_261 at 325.696 kb on + strand, within Pf6N2E2_261 at 325.696 kb on + strand, within Pf6N2E2_261 at 325.696 kb on + strand, within Pf6N2E2_261 at 325.697 kb on - strand, within Pf6N2E2_261 at 325.697 kb on - strand, within Pf6N2E2_261 at 325.697 kb on - strand, within Pf6N2E2_261 at 325.702 kb on + strand, within Pf6N2E2_261 at 325.702 kb on + strand, within Pf6N2E2_261 at 325.702 kb on + strand, within Pf6N2E2_261 at 325.702 kb on + strand, within Pf6N2E2_261 at 325.703 kb on - strand, within Pf6N2E2_261 at 325.703 kb on - strand, within Pf6N2E2_261 at 325.703 kb on - strand, within Pf6N2E2_261 at 325.703 kb on - strand, within Pf6N2E2_261 at 325.759 kb on + strand, within Pf6N2E2_261 at 325.759 kb on + strand, within Pf6N2E2_261 at 325.759 kb on + strand, within Pf6N2E2_261 at 325.759 kb on + strand, within Pf6N2E2_261 at 325.759 kb on + strand, within Pf6N2E2_261 at 325.759 kb on + strand, within Pf6N2E2_261 at 325.759 kb on + strand, within Pf6N2E2_261 at 325.760 kb on - strand, within Pf6N2E2_261 at 325.760 kb on - strand, within Pf6N2E2_261 at 325.760 kb on - strand, within Pf6N2E2_261 at 325.760 kb on - strand, within Pf6N2E2_261 at 325.760 kb on - strand, within Pf6N2E2_261 at 325.760 kb on - strand, within Pf6N2E2_261 at 325.760 kb on - strand, within Pf6N2E2_261 at 325.760 kb on - strand, within Pf6N2E2_261 at 325.760 kb on - strand, within Pf6N2E2_261 at 325.760 kb on - strand, within Pf6N2E2_261 at 325.760 kb on - strand, within Pf6N2E2_261 at 325.760 kb on - strand, within Pf6N2E2_261 at 325.760 kb on - strand, within Pf6N2E2_261 at 325.965 kb on + strand at 326.096 kb on + strand, within Pf6N2E2_262 at 326.097 kb on - strand, within Pf6N2E2_262 at 326.253 kb on - strand, within Pf6N2E2_262 at 326.265 kb on + strand at 326.266 kb on - strand at 326.465 kb on + strand at 326.465 kb on + strand at 326.465 kb on + strand at 326.465 kb on + strand at 326.466 kb on - strand at 326.466 kb on - strand at 326.466 kb on - strand at 326.466 kb on - strand at 326.553 kb on + strand at 326.553 kb on + strand at 326.554 kb on - strand at 326.554 kb on - strand at 326.554 kb on - strand at 326.558 kb on - strand at 326.558 kb on - strand at 326.644 kb on + strand, within Pf6N2E2_263 at 326.644 kb on + strand, within Pf6N2E2_263 at 326.644 kb on + strand, within Pf6N2E2_263 at 326.644 kb on + strand, within Pf6N2E2_263 at 326.645 kb on - strand, within Pf6N2E2_263 at 326.645 kb on - strand, within Pf6N2E2_263 at 326.645 kb on - strand, within Pf6N2E2_263 at 326.645 kb on - strand, within Pf6N2E2_263 at 326.737 kb on + strand, within Pf6N2E2_263 at 326.738 kb on - strand, within Pf6N2E2_263 at 326.738 kb on - strand, within Pf6N2E2_263 at 326.738 kb on - strand, within Pf6N2E2_263 at 326.738 kb on - strand, within Pf6N2E2_263 at 326.784 kb on + strand, within Pf6N2E2_263 at 326.849 kb on + strand, within Pf6N2E2_263 at 326.850 kb on - strand, within Pf6N2E2_263
Per-strain Table
Position Strand Gene LocusTag Fraction 50% P. fluorescens FW300-N2E2 spent media from growth in 20 mM putrescine remove 323,914 + Pf6N2E2_259 0.81 -0.2 323,914 + Pf6N2E2_259 0.81 +0.0 323,914 + Pf6N2E2_259 0.81 +0.8 323,915 - Pf6N2E2_259 0.81 +1.2 323,915 - Pf6N2E2_259 0.81 +0.8 323,915 - Pf6N2E2_259 0.81 -0.2 323,915 - Pf6N2E2_259 0.81 -0.2 323,915 - Pf6N2E2_259 0.81 -0.8 324,083 - -0.2 324,083 - -1.8 324,128 + -0.0 324,128 + +0.2 324,129 - +0.9 324,179 - -0.1 324,383 + Pf6N2E2_260 0.51 +0.8 324,406 + Pf6N2E2_260 0.56 +0.2 324,406 + Pf6N2E2_260 0.56 +0.3 324,407 - Pf6N2E2_260 0.56 -0.7 324,409 + Pf6N2E2_260 0.56 -0.4 324,409 + Pf6N2E2_260 0.56 +1.3 324,410 - Pf6N2E2_260 0.56 -0.2 324,617 + -0.5 324,617 + -0.1 324,617 + -0.4 324,617 + +0.3 324,617 + -0.2 324,617 + +2.0 324,617 + +0.3 324,618 - +0.4 324,618 - -0.2 324,618 - -1.2 324,618 - -0.0 324,621 + -1.2 324,621 + +0.1 324,621 + -0.2 324,621 + +0.1 324,621 + -0.2 324,635 + -0.1 325,034 - Pf6N2E2_261 0.20 +0.2 325,228 + Pf6N2E2_261 0.37 +0.3 325,228 + Pf6N2E2_261 0.37 -0.2 325,229 - Pf6N2E2_261 0.37 +0.7 325,408 - Pf6N2E2_261 0.53 +0.8 325,507 + Pf6N2E2_261 0.62 +1.2 325,507 + Pf6N2E2_261 0.62 +0.1 325,508 - Pf6N2E2_261 0.62 -0.2 325,609 - Pf6N2E2_261 0.71 -1.6 325,609 - Pf6N2E2_261 0.71 +0.2 325,696 + Pf6N2E2_261 0.79 -0.1 325,696 + Pf6N2E2_261 0.79 -0.4 325,696 + Pf6N2E2_261 0.79 -0.2 325,697 - Pf6N2E2_261 0.79 -0.2 325,697 - Pf6N2E2_261 0.79 +0.3 325,697 - Pf6N2E2_261 0.79 -0.9 325,702 + Pf6N2E2_261 0.80 +0.0 325,702 + Pf6N2E2_261 0.80 -0.2 325,702 + Pf6N2E2_261 0.80 -0.2 325,702 + Pf6N2E2_261 0.80 +1.8 325,703 - Pf6N2E2_261 0.80 +0.0 325,703 - Pf6N2E2_261 0.80 +1.3 325,703 - Pf6N2E2_261 0.80 +0.1 325,703 - Pf6N2E2_261 0.80 +1.8 325,759 + Pf6N2E2_261 0.85 -0.2 325,759 + Pf6N2E2_261 0.85 +0.3 325,759 + Pf6N2E2_261 0.85 +0.2 325,759 + Pf6N2E2_261 0.85 +0.9 325,759 + Pf6N2E2_261 0.85 -0.2 325,759 + Pf6N2E2_261 0.85 +0.5 325,759 + Pf6N2E2_261 0.85 +1.5 325,760 - Pf6N2E2_261 0.85 +0.8 325,760 - Pf6N2E2_261 0.85 -1.8 325,760 - Pf6N2E2_261 0.85 -0.5 325,760 - Pf6N2E2_261 0.85 -1.8 325,760 - Pf6N2E2_261 0.85 +0.2 325,760 - Pf6N2E2_261 0.85 +0.1 325,760 - Pf6N2E2_261 0.85 +2.6 325,760 - Pf6N2E2_261 0.85 -0.2 325,760 - Pf6N2E2_261 0.85 -0.4 325,760 - Pf6N2E2_261 0.85 +0.8 325,760 - Pf6N2E2_261 0.85 +0.3 325,760 - Pf6N2E2_261 0.85 -0.2 325,760 - Pf6N2E2_261 0.85 +1.8 325,965 + -0.6 326,096 + Pf6N2E2_262 0.34 +0.3 326,097 - Pf6N2E2_262 0.34 +0.3 326,253 - Pf6N2E2_262 0.87 -0.2 326,265 + +1.1 326,266 - +1.9 326,465 + +1.3 326,465 + +0.8 326,465 + -0.2 326,465 + +0.3 326,466 - +1.1 326,466 - +0.9 326,466 - -0.2 326,466 - -0.6 326,553 + +0.3 326,553 + -0.2 326,554 - +0.0 326,554 - +0.2 326,554 - +0.8 326,558 - -1.8 326,558 - +0.6 326,644 + Pf6N2E2_263 0.14 +0.5 326,644 + Pf6N2E2_263 0.14 +0.2 326,644 + Pf6N2E2_263 0.14 +0.6 326,644 + Pf6N2E2_263 0.14 -0.2 326,645 - Pf6N2E2_263 0.14 -0.7 326,645 - Pf6N2E2_263 0.14 +0.1 326,645 - Pf6N2E2_263 0.14 -1.8 326,645 - Pf6N2E2_263 0.14 +1.5 326,737 + Pf6N2E2_263 0.28 +0.3 326,738 - Pf6N2E2_263 0.28 +1.1 326,738 - Pf6N2E2_263 0.28 -0.7 326,738 - Pf6N2E2_263 0.28 +0.8 326,738 - Pf6N2E2_263 0.28 +1.0 326,784 + Pf6N2E2_263 0.35 +0.8 326,849 + Pf6N2E2_263 0.45 -0.8 326,850 - Pf6N2E2_263 0.46 -0.2
Or see this region's nucleotide sequence