Experiment: 50% P. fluorescens FW300-N2E2 spent media from growth in 20 mM putrescine
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt Pf6N2E2_1904 and Pf6N2E2_1905 are separated by 129 nucleotides Pf6N2E2_1905 and Pf6N2E2_1906 are separated by 272 nucleotides Pf6N2E2_1906 and Pf6N2E2_1907 are separated by 72 nucleotides
Pf6N2E2_1904 - Isoquinoline 1-oxidoreductase beta subunit (EC 1.3.99.16), at 2,255,744 to 2,258,002
_1904
Pf6N2E2_1905 - Isoquinoline 1-oxidoreductase alpha subunit (EC 1.3.99.16), at 2,258,132 to 2,258,578
_1905
Pf6N2E2_1906 - Aldehyde dehydrogenase (EC 1.2.1.3), at 2,258,851 to 2,260,302
_1906
Pf6N2E2_1907 - Choline dehydrogenase (EC 1.1.99.1), at 2,260,375 to 2,262,015
_1907
Position (kb)
2258
2259
2260
2261 Strain fitness (log2 ratio)
-4
-3
-2
-1
0
1
2
3 at 2258.002 kb on + strand at 2258.002 kb on + strand at 2258.002 kb on + strand at 2258.003 kb on - strand at 2258.003 kb on - strand at 2258.064 kb on - strand at 2258.174 kb on - strand at 2258.174 kb on - strand at 2258.175 kb on + strand at 2258.175 kb on + strand at 2258.176 kb on - strand at 2258.176 kb on - strand at 2258.475 kb on + strand, within Pf6N2E2_1905 at 2258.475 kb on + strand, within Pf6N2E2_1905 at 2258.476 kb on - strand, within Pf6N2E2_1905 at 2258.476 kb on - strand, within Pf6N2E2_1905 at 2258.476 kb on - strand, within Pf6N2E2_1905 at 2258.516 kb on + strand, within Pf6N2E2_1905 at 2258.516 kb on + strand, within Pf6N2E2_1905 at 2258.528 kb on - strand, within Pf6N2E2_1905 at 2258.550 kb on + strand at 2258.550 kb on + strand at 2258.551 kb on - strand at 2258.551 kb on - strand at 2258.551 kb on - strand at 2258.564 kb on + strand at 2258.581 kb on + strand at 2258.582 kb on - strand at 2258.582 kb on - strand at 2258.582 kb on - strand at 2258.582 kb on - strand at 2258.583 kb on - strand at 2258.691 kb on - strand at 2258.706 kb on + strand at 2258.762 kb on + strand at 2258.763 kb on - strand at 2258.763 kb on - strand at 2258.778 kb on - strand at 2258.781 kb on + strand at 2258.815 kb on - strand at 2258.898 kb on - strand at 2258.969 kb on + strand at 2258.970 kb on - strand at 2258.995 kb on - strand at 2259.009 kb on + strand, within Pf6N2E2_1906 at 2259.051 kb on - strand, within Pf6N2E2_1906 at 2259.051 kb on - strand, within Pf6N2E2_1906 at 2259.051 kb on - strand, within Pf6N2E2_1906 at 2259.076 kb on + strand, within Pf6N2E2_1906 at 2259.076 kb on + strand at 2259.077 kb on - strand, within Pf6N2E2_1906 at 2259.077 kb on - strand, within Pf6N2E2_1906 at 2259.077 kb on - strand, within Pf6N2E2_1906 at 2259.080 kb on + strand, within Pf6N2E2_1906 at 2259.080 kb on + strand, within Pf6N2E2_1906 at 2259.080 kb on + strand, within Pf6N2E2_1906 at 2259.081 kb on - strand, within Pf6N2E2_1906 at 2259.081 kb on - strand, within Pf6N2E2_1906 at 2259.081 kb on - strand, within Pf6N2E2_1906 at 2259.081 kb on - strand, within Pf6N2E2_1906 at 2259.081 kb on - strand, within Pf6N2E2_1906 at 2259.186 kb on + strand, within Pf6N2E2_1906 at 2259.187 kb on - strand, within Pf6N2E2_1906 at 2259.187 kb on - strand, within Pf6N2E2_1906 at 2259.187 kb on - strand, within Pf6N2E2_1906 at 2259.187 kb on - strand, within Pf6N2E2_1906 at 2259.308 kb on - strand, within Pf6N2E2_1906 at 2259.308 kb on - strand, within Pf6N2E2_1906 at 2259.308 kb on - strand, within Pf6N2E2_1906 at 2259.308 kb on - strand, within Pf6N2E2_1906 at 2259.308 kb on - strand, within Pf6N2E2_1906 at 2259.308 kb on - strand, within Pf6N2E2_1906 at 2259.308 kb on - strand, within Pf6N2E2_1906 at 2259.308 kb on - strand, within Pf6N2E2_1906 at 2259.308 kb on - strand, within Pf6N2E2_1906 at 2259.308 kb on - strand, within Pf6N2E2_1906 at 2259.319 kb on + strand, within Pf6N2E2_1906 at 2259.319 kb on + strand, within Pf6N2E2_1906 at 2259.319 kb on + strand, within Pf6N2E2_1906 at 2259.319 kb on + strand, within Pf6N2E2_1906 at 2259.319 kb on + strand, within Pf6N2E2_1906 at 2259.319 kb on + strand, within Pf6N2E2_1906 at 2259.319 kb on + strand, within Pf6N2E2_1906 at 2259.319 kb on + strand, within Pf6N2E2_1906 at 2259.319 kb on + strand, within Pf6N2E2_1906 at 2259.320 kb on - strand, within Pf6N2E2_1906 at 2259.320 kb on - strand, within Pf6N2E2_1906 at 2259.320 kb on - strand, within Pf6N2E2_1906 at 2259.553 kb on - strand, within Pf6N2E2_1906 at 2259.772 kb on - strand, within Pf6N2E2_1906 at 2259.814 kb on - strand, within Pf6N2E2_1906 at 2259.814 kb on - strand, within Pf6N2E2_1906 at 2259.814 kb on - strand, within Pf6N2E2_1906 at 2259.814 kb on - strand, within Pf6N2E2_1906 at 2259.833 kb on + strand, within Pf6N2E2_1906 at 2259.833 kb on + strand, within Pf6N2E2_1906 at 2259.834 kb on - strand, within Pf6N2E2_1906 at 2259.834 kb on - strand, within Pf6N2E2_1906 at 2259.922 kb on - strand, within Pf6N2E2_1906 at 2259.948 kb on - strand, within Pf6N2E2_1906 at 2259.978 kb on + strand, within Pf6N2E2_1906 at 2259.978 kb on + strand, within Pf6N2E2_1906 at 2259.978 kb on + strand, within Pf6N2E2_1906 at 2259.978 kb on + strand, within Pf6N2E2_1906 at 2259.978 kb on + strand, within Pf6N2E2_1906 at 2259.978 kb on + strand, within Pf6N2E2_1906 at 2259.979 kb on - strand, within Pf6N2E2_1906 at 2259.979 kb on - strand, within Pf6N2E2_1906 at 2259.979 kb on - strand, within Pf6N2E2_1906 at 2259.979 kb on - strand, within Pf6N2E2_1906 at 2259.979 kb on - strand, within Pf6N2E2_1906 at 2259.979 kb on - strand, within Pf6N2E2_1906 at 2259.979 kb on - strand, within Pf6N2E2_1906 at 2259.979 kb on - strand, within Pf6N2E2_1906 at 2259.979 kb on - strand, within Pf6N2E2_1906 at 2259.979 kb on - strand, within Pf6N2E2_1906 at 2260.024 kb on - strand, within Pf6N2E2_1906 at 2260.073 kb on + strand, within Pf6N2E2_1906 at 2260.086 kb on + strand, within Pf6N2E2_1906 at 2260.086 kb on + strand, within Pf6N2E2_1906 at 2260.086 kb on + strand, within Pf6N2E2_1906 at 2260.087 kb on - strand, within Pf6N2E2_1906 at 2260.130 kb on + strand, within Pf6N2E2_1906 at 2260.278 kb on + strand at 2260.279 kb on - strand at 2260.638 kb on - strand, within Pf6N2E2_1907 at 2260.638 kb on - strand, within Pf6N2E2_1907 at 2260.643 kb on + strand, within Pf6N2E2_1907 at 2260.643 kb on + strand, within Pf6N2E2_1907 at 2260.643 kb on + strand, within Pf6N2E2_1907 at 2260.644 kb on - strand, within Pf6N2E2_1907 at 2260.644 kb on - strand, within Pf6N2E2_1907 at 2260.644 kb on - strand, within Pf6N2E2_1907 at 2260.644 kb on - strand, within Pf6N2E2_1907 at 2260.647 kb on + strand, within Pf6N2E2_1907 at 2260.647 kb on + strand, within Pf6N2E2_1907 at 2260.797 kb on - strand, within Pf6N2E2_1907 at 2260.866 kb on - strand, within Pf6N2E2_1907 at 2260.964 kb on + strand, within Pf6N2E2_1907 at 2260.965 kb on - strand, within Pf6N2E2_1907 at 2261.063 kb on + strand, within Pf6N2E2_1907 at 2261.064 kb on - strand, within Pf6N2E2_1907 at 2261.077 kb on + strand, within Pf6N2E2_1907 at 2261.078 kb on - strand, within Pf6N2E2_1907 at 2261.078 kb on - strand, within Pf6N2E2_1907 at 2261.078 kb on - strand, within Pf6N2E2_1907 at 2261.093 kb on + strand, within Pf6N2E2_1907 at 2261.094 kb on - strand, within Pf6N2E2_1907 at 2261.094 kb on - strand, within Pf6N2E2_1907 at 2261.133 kb on - strand, within Pf6N2E2_1907 at 2261.289 kb on - strand, within Pf6N2E2_1907 at 2261.289 kb on - strand, within Pf6N2E2_1907 at 2261.289 kb on - strand, within Pf6N2E2_1907 at 2261.293 kb on - strand, within Pf6N2E2_1907 at 2261.293 kb on - strand, within Pf6N2E2_1907 at 2261.293 kb on - strand, within Pf6N2E2_1907 at 2261.293 kb on - strand, within Pf6N2E2_1907 at 2261.293 kb on - strand, within Pf6N2E2_1907 at 2261.293 kb on - strand, within Pf6N2E2_1907 at 2261.293 kb on - strand, within Pf6N2E2_1907
Per-strain Table
Position Strand Gene LocusTag Fraction 50% P. fluorescens FW300-N2E2 spent media from growth in 20 mM putrescine remove 2,258,002 + +0.3 2,258,002 + -0.3 2,258,002 + -1.2 2,258,003 - +0.3 2,258,003 - -0.3 2,258,064 - -0.3 2,258,174 - +1.7 2,258,174 - +0.4 2,258,175 + -1.3 2,258,175 + -1.3 2,258,176 - -1.3 2,258,176 - +1.1 2,258,475 + Pf6N2E2_1905 0.77 -0.7 2,258,475 + Pf6N2E2_1905 0.77 -0.3 2,258,476 - Pf6N2E2_1905 0.77 +1.3 2,258,476 - Pf6N2E2_1905 0.77 +1.7 2,258,476 - Pf6N2E2_1905 0.77 +1.3 2,258,516 + Pf6N2E2_1905 0.86 +1.4 2,258,516 + Pf6N2E2_1905 0.86 -0.8 2,258,528 - Pf6N2E2_1905 0.89 +0.7 2,258,550 + +1.0 2,258,550 + +3.0 2,258,551 - +0.7 2,258,551 - -0.3 2,258,551 - -0.3 2,258,564 + +0.3 2,258,581 + -0.8 2,258,582 - -0.7 2,258,582 - +0.4 2,258,582 - +0.8 2,258,582 - +1.0 2,258,583 - +0.9 2,258,691 - -0.2 2,258,706 + +0.5 2,258,762 + +0.4 2,258,763 - +1.7 2,258,763 - -0.9 2,258,778 - +0.3 2,258,781 + +0.7 2,258,815 - +0.9 2,258,898 - +1.4 2,258,969 + -0.1 2,258,970 - +1.7 2,258,995 - +1.2 2,259,009 + Pf6N2E2_1906 0.11 +1.3 2,259,051 - Pf6N2E2_1906 0.14 +1.1 2,259,051 - Pf6N2E2_1906 0.14 +2.5 2,259,051 - Pf6N2E2_1906 0.14 +0.8 2,259,076 + Pf6N2E2_1906 0.15 -0.3 2,259,076 + +2.0 2,259,077 - Pf6N2E2_1906 0.16 +0.3 2,259,077 - Pf6N2E2_1906 0.16 +0.7 2,259,077 - Pf6N2E2_1906 0.16 +2.7 2,259,080 + Pf6N2E2_1906 0.16 +0.3 2,259,080 + Pf6N2E2_1906 0.16 +0.7 2,259,080 + Pf6N2E2_1906 0.16 +1.3 2,259,081 - Pf6N2E2_1906 0.16 +1.0 2,259,081 - Pf6N2E2_1906 0.16 +1.1 2,259,081 - Pf6N2E2_1906 0.16 +1.0 2,259,081 - Pf6N2E2_1906 0.16 -0.3 2,259,081 - Pf6N2E2_1906 0.16 +1.3 2,259,186 + Pf6N2E2_1906 0.23 +1.4 2,259,187 - Pf6N2E2_1906 0.23 +0.1 2,259,187 - Pf6N2E2_1906 0.23 +0.7 2,259,187 - Pf6N2E2_1906 0.23 -0.1 2,259,187 - Pf6N2E2_1906 0.23 +0.9 2,259,308 - Pf6N2E2_1906 0.31 -0.6 2,259,308 - Pf6N2E2_1906 0.31 +0.7 2,259,308 - Pf6N2E2_1906 0.31 +1.1 2,259,308 - Pf6N2E2_1906 0.31 +1.7 2,259,308 - Pf6N2E2_1906 0.31 -0.2 2,259,308 - Pf6N2E2_1906 0.31 +0.3 2,259,308 - Pf6N2E2_1906 0.31 -0.2 2,259,308 - Pf6N2E2_1906 0.31 +0.6 2,259,308 - Pf6N2E2_1906 0.31 +1.4 2,259,308 - Pf6N2E2_1906 0.31 +0.6 2,259,319 + Pf6N2E2_1906 0.32 +0.4 2,259,319 + Pf6N2E2_1906 0.32 +1.3 2,259,319 + Pf6N2E2_1906 0.32 +1.2 2,259,319 + Pf6N2E2_1906 0.32 -3.8 2,259,319 + Pf6N2E2_1906 0.32 +0.6 2,259,319 + Pf6N2E2_1906 0.32 -0.8 2,259,319 + Pf6N2E2_1906 0.32 -0.0 2,259,319 + Pf6N2E2_1906 0.32 +0.3 2,259,319 + Pf6N2E2_1906 0.32 +0.4 2,259,320 - Pf6N2E2_1906 0.32 +0.9 2,259,320 - Pf6N2E2_1906 0.32 -0.3 2,259,320 - Pf6N2E2_1906 0.32 +1.5 2,259,553 - Pf6N2E2_1906 0.48 -0.7 2,259,772 - Pf6N2E2_1906 0.63 +0.6 2,259,814 - Pf6N2E2_1906 0.66 +0.7 2,259,814 - Pf6N2E2_1906 0.66 +0.4 2,259,814 - Pf6N2E2_1906 0.66 +1.6 2,259,814 - Pf6N2E2_1906 0.66 +1.3 2,259,833 + Pf6N2E2_1906 0.68 +0.6 2,259,833 + Pf6N2E2_1906 0.68 +0.7 2,259,834 - Pf6N2E2_1906 0.68 -0.3 2,259,834 - Pf6N2E2_1906 0.68 -2.7 2,259,922 - Pf6N2E2_1906 0.74 -0.3 2,259,948 - Pf6N2E2_1906 0.76 -0.3 2,259,978 + Pf6N2E2_1906 0.78 +1.7 2,259,978 + Pf6N2E2_1906 0.78 -0.1 2,259,978 + Pf6N2E2_1906 0.78 +0.4 2,259,978 + Pf6N2E2_1906 0.78 +0.4 2,259,978 + Pf6N2E2_1906 0.78 -0.8 2,259,978 + Pf6N2E2_1906 0.78 +0.1 2,259,979 - Pf6N2E2_1906 0.78 +1.3 2,259,979 - Pf6N2E2_1906 0.78 +0.4 2,259,979 - Pf6N2E2_1906 0.78 +0.7 2,259,979 - Pf6N2E2_1906 0.78 -0.7 2,259,979 - Pf6N2E2_1906 0.78 +0.2 2,259,979 - Pf6N2E2_1906 0.78 +0.8 2,259,979 - Pf6N2E2_1906 0.78 -0.2 2,259,979 - Pf6N2E2_1906 0.78 +0.3 2,259,979 - Pf6N2E2_1906 0.78 -0.6 2,259,979 - Pf6N2E2_1906 0.78 -1.3 2,260,024 - Pf6N2E2_1906 0.81 -0.3 2,260,073 + Pf6N2E2_1906 0.84 -0.3 2,260,086 + Pf6N2E2_1906 0.85 -0.1 2,260,086 + Pf6N2E2_1906 0.85 +1.3 2,260,086 + Pf6N2E2_1906 0.85 -0.5 2,260,087 - Pf6N2E2_1906 0.85 -0.3 2,260,130 + Pf6N2E2_1906 0.88 -0.9 2,260,278 + +0.9 2,260,279 - +0.0 2,260,638 - Pf6N2E2_1907 0.16 -2.3 2,260,638 - Pf6N2E2_1907 0.16 -0.3 2,260,643 + Pf6N2E2_1907 0.16 +0.2 2,260,643 + Pf6N2E2_1907 0.16 +1.2 2,260,643 + Pf6N2E2_1907 0.16 +1.0 2,260,644 - Pf6N2E2_1907 0.16 +0.4 2,260,644 - Pf6N2E2_1907 0.16 +1.8 2,260,644 - Pf6N2E2_1907 0.16 +1.0 2,260,644 - Pf6N2E2_1907 0.16 +0.1 2,260,647 + Pf6N2E2_1907 0.17 +1.3 2,260,647 + Pf6N2E2_1907 0.17 +0.8 2,260,797 - Pf6N2E2_1907 0.26 +1.3 2,260,866 - Pf6N2E2_1907 0.30 +0.8 2,260,964 + Pf6N2E2_1907 0.36 +1.0 2,260,965 - Pf6N2E2_1907 0.36 +2.5 2,261,063 + Pf6N2E2_1907 0.42 +1.1 2,261,064 - Pf6N2E2_1907 0.42 +0.8 2,261,077 + Pf6N2E2_1907 0.43 -0.3 2,261,078 - Pf6N2E2_1907 0.43 +0.7 2,261,078 - Pf6N2E2_1907 0.43 -0.3 2,261,078 - Pf6N2E2_1907 0.43 +0.1 2,261,093 + Pf6N2E2_1907 0.44 -0.6 2,261,094 - Pf6N2E2_1907 0.44 -0.3 2,261,094 - Pf6N2E2_1907 0.44 +1.2 2,261,133 - Pf6N2E2_1907 0.46 +0.3 2,261,289 - Pf6N2E2_1907 0.56 +0.4 2,261,289 - Pf6N2E2_1907 0.56 +0.7 2,261,289 - Pf6N2E2_1907 0.56 +1.3 2,261,293 - Pf6N2E2_1907 0.56 -1.9 2,261,293 - Pf6N2E2_1907 0.56 -1.3 2,261,293 - Pf6N2E2_1907 0.56 -0.1 2,261,293 - Pf6N2E2_1907 0.56 -0.3 2,261,293 - Pf6N2E2_1907 0.56 -1.3 2,261,293 - Pf6N2E2_1907 0.56 +1.3 2,261,293 - Pf6N2E2_1907 0.56 +0.7
Or see this region's nucleotide sequence