Experiment: 50% P. fluorescens FW300-N2E2 spent media from growth in 20 mM putrescine
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt Pf6N2E2_1878 and Pf6N2E2_1879 are separated by 18 nucleotides
Pf6N2E2_1878 - Aldehyde dehydrogenase (EC 1.2.1.3), at 2,229,653 to 2,231,071
_1878
Pf6N2E2_1879 - Long-chain-fatty-acid--CoA ligase (EC 6.2.1.3), at 2,231,090 to 2,232,625
_1879
Position (kb)
2229
2230
2231
2232 Strain fitness (log2 ratio)
-2
-1
0
1
2 at 2228.660 kb on - strand at 2228.702 kb on + strand at 2228.833 kb on + strand at 2228.833 kb on + strand at 2228.834 kb on - strand at 2228.834 kb on - strand at 2228.834 kb on - strand at 2228.834 kb on - strand at 2228.834 kb on - strand at 2228.837 kb on + strand at 2228.838 kb on - strand at 2228.838 kb on - strand at 2228.841 kb on + strand at 2228.841 kb on + strand at 2228.842 kb on - strand at 2228.923 kb on + strand at 2228.924 kb on - strand at 2228.924 kb on - strand at 2229.075 kb on + strand at 2229.076 kb on - strand at 2229.096 kb on - strand at 2229.112 kb on + strand at 2229.112 kb on + strand at 2229.112 kb on + strand at 2229.112 kb on + strand at 2229.119 kb on + strand at 2229.120 kb on - strand at 2229.152 kb on + strand at 2229.200 kb on + strand at 2229.200 kb on + strand at 2229.201 kb on - strand at 2229.201 kb on - strand at 2229.212 kb on + strand at 2229.212 kb on + strand at 2229.212 kb on + strand at 2229.212 kb on + strand at 2229.213 kb on - strand at 2229.213 kb on - strand at 2229.213 kb on - strand at 2229.213 kb on - strand at 2229.213 kb on - strand at 2229.213 kb on - strand at 2229.286 kb on - strand at 2229.286 kb on - strand at 2229.286 kb on - strand at 2229.286 kb on - strand at 2229.286 kb on - strand at 2229.315 kb on - strand at 2229.347 kb on - strand at 2229.347 kb on - strand at 2229.374 kb on - strand at 2229.374 kb on - strand at 2229.374 kb on - strand at 2229.424 kb on - strand at 2229.425 kb on + strand at 2229.425 kb on + strand at 2229.445 kb on - strand at 2229.484 kb on + strand at 2229.485 kb on - strand at 2229.485 kb on - strand at 2229.485 kb on - strand at 2229.498 kb on + strand at 2229.511 kb on + strand at 2229.511 kb on + strand at 2229.597 kb on + strand at 2229.598 kb on - strand at 2229.598 kb on - strand at 2229.598 kb on - strand at 2229.670 kb on + strand at 2229.745 kb on + strand at 2229.746 kb on - strand at 2229.877 kb on + strand, within Pf6N2E2_1878 at 2230.027 kb on - strand, within Pf6N2E2_1878 at 2230.144 kb on + strand, within Pf6N2E2_1878 at 2230.186 kb on + strand, within Pf6N2E2_1878 at 2230.187 kb on - strand, within Pf6N2E2_1878 at 2230.272 kb on + strand, within Pf6N2E2_1878 at 2230.272 kb on + strand, within Pf6N2E2_1878 at 2230.273 kb on - strand, within Pf6N2E2_1878 at 2230.407 kb on + strand, within Pf6N2E2_1878 at 2230.407 kb on + strand, within Pf6N2E2_1878 at 2230.408 kb on - strand, within Pf6N2E2_1878 at 2230.531 kb on - strand, within Pf6N2E2_1878 at 2230.644 kb on + strand, within Pf6N2E2_1878 at 2230.645 kb on - strand, within Pf6N2E2_1878 at 2230.768 kb on - strand, within Pf6N2E2_1878 at 2230.824 kb on + strand, within Pf6N2E2_1878 at 2230.824 kb on + strand, within Pf6N2E2_1878 at 2230.824 kb on + strand, within Pf6N2E2_1878 at 2230.876 kb on + strand, within Pf6N2E2_1878 at 2231.638 kb on + strand, within Pf6N2E2_1879 at 2231.847 kb on + strand, within Pf6N2E2_1879 at 2231.867 kb on + strand, within Pf6N2E2_1879 at 2232.013 kb on + strand, within Pf6N2E2_1879 at 2232.053 kb on + strand, within Pf6N2E2_1879 at 2232.053 kb on + strand, within Pf6N2E2_1879 at 2232.053 kb on + strand, within Pf6N2E2_1879 at 2232.054 kb on - strand, within Pf6N2E2_1879
Per-strain Table
Position Strand Gene LocusTag Fraction 50% P. fluorescens FW300-N2E2 spent media from growth in 20 mM putrescine remove 2,228,660 - +0.4 2,228,702 + +1.4 2,228,833 + -0.1 2,228,833 + -0.3 2,228,834 - +0.7 2,228,834 - +0.3 2,228,834 - +0.3 2,228,834 - +1.0 2,228,834 - -0.7 2,228,837 + +1.7 2,228,838 - +0.4 2,228,838 - +2.5 2,228,841 + -0.3 2,228,841 + +1.7 2,228,842 - +2.0 2,228,923 + -0.3 2,228,924 - +0.5 2,228,924 - -0.3 2,229,075 + +0.3 2,229,076 - +0.3 2,229,096 - +0.6 2,229,112 + -0.3 2,229,112 + -0.9 2,229,112 + +0.7 2,229,112 + -0.3 2,229,119 + +0.4 2,229,120 - +1.0 2,229,152 + +0.7 2,229,200 + -1.3 2,229,200 + -1.9 2,229,201 - -0.3 2,229,201 - +0.0 2,229,212 + -0.9 2,229,212 + +0.5 2,229,212 + -0.6 2,229,212 + -0.3 2,229,213 - -0.3 2,229,213 - -0.3 2,229,213 - -0.3 2,229,213 - -0.3 2,229,213 - -0.8 2,229,213 - +1.7 2,229,286 - -1.3 2,229,286 - +0.7 2,229,286 - +0.3 2,229,286 - -0.5 2,229,286 - -0.3 2,229,315 - -0.3 2,229,347 - +0.3 2,229,347 - -0.1 2,229,374 - -0.3 2,229,374 - -0.3 2,229,374 - -1.3 2,229,424 - +0.5 2,229,425 + +0.9 2,229,425 + -0.1 2,229,445 - +0.7 2,229,484 + -1.3 2,229,485 - +0.2 2,229,485 - -0.3 2,229,485 - -0.6 2,229,498 + -0.7 2,229,511 + +0.9 2,229,511 + -0.7 2,229,597 + +0.9 2,229,598 - +0.4 2,229,598 - +0.7 2,229,598 - +0.3 2,229,670 + -1.3 2,229,745 + -0.3 2,229,746 - -1.3 2,229,877 + Pf6N2E2_1878 0.16 +1.3 2,230,027 - Pf6N2E2_1878 0.26 -0.3 2,230,144 + Pf6N2E2_1878 0.35 -0.3 2,230,186 + Pf6N2E2_1878 0.38 +0.2 2,230,187 - Pf6N2E2_1878 0.38 +1.0 2,230,272 + Pf6N2E2_1878 0.44 -0.3 2,230,272 + Pf6N2E2_1878 0.44 -0.1 2,230,273 - Pf6N2E2_1878 0.44 +1.7 2,230,407 + Pf6N2E2_1878 0.53 +1.3 2,230,407 + Pf6N2E2_1878 0.53 +1.7 2,230,408 - Pf6N2E2_1878 0.53 +0.4 2,230,531 - Pf6N2E2_1878 0.62 +0.7 2,230,644 + Pf6N2E2_1878 0.70 +0.7 2,230,645 - Pf6N2E2_1878 0.70 -0.7 2,230,768 - Pf6N2E2_1878 0.79 +0.4 2,230,824 + Pf6N2E2_1878 0.83 -0.6 2,230,824 + Pf6N2E2_1878 0.83 -0.3 2,230,824 + Pf6N2E2_1878 0.83 -0.9 2,230,876 + Pf6N2E2_1878 0.86 +0.9 2,231,638 + Pf6N2E2_1879 0.36 -0.6 2,231,847 + Pf6N2E2_1879 0.49 +2.1 2,231,867 + Pf6N2E2_1879 0.51 -0.3 2,232,013 + Pf6N2E2_1879 0.60 +0.6 2,232,053 + Pf6N2E2_1879 0.63 -0.3 2,232,053 + Pf6N2E2_1879 0.63 -0.3 2,232,053 + Pf6N2E2_1879 0.63 +0.5 2,232,054 - Pf6N2E2_1879 0.63 -0.9
Or see this region's nucleotide sequence