Strain Fitness in Pseudomonas fluorescens FW300-N2E2 around Pf6N2E2_1874

Experiment: 50% P. fluorescens FW300-N2E2 spent media from growth in 20 mM putrescine

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Only strains with sufficient reads to estimate fitness are shown, but the strain fitness values are still rather noisy and may be biased towards zero. Strains near the edge of a gene are not shown as being associated with that gene (they are in grey). Strains in the central 10-90% of a gene are color coded by the insertion's strand. Usually, "+" means that the selectable marker is encoded on the forward strand, i.e., transcribed rightward.

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500 ntPf6N2E2_1873 and Pf6N2E2_1874 are separated by 911 nucleotidesPf6N2E2_1874 and Pf6N2E2_1875 are separated by 50 nucleotides Pf6N2E2_1873 - Amidase, at 2,219,209 to 2,220,639 _1873 Pf6N2E2_1874 - Aldehyde dehydrogenase (EC 1.2.1.3), at 2,221,551 to 2,222,990 _1874 Pf6N2E2_1875 - Protoporphyrinogen oxidase (EC 1.3.3.4), at 2,223,041 to 2,224,369 _1875 Position (kb) 2221 2222 2223Strain fitness (log2 ratio) -2 -1 0 1at 2220.643 kb on - strandat 2220.780 kb on - strandat 2220.934 kb on - strandat 2220.956 kb on - strandat 2220.956 kb on - strandat 2220.956 kb on - strandat 2220.991 kb on - strandat 2221.068 kb on - strandat 2221.132 kb on - strandat 2221.190 kb on + strandat 2221.191 kb on - strandat 2221.669 kb on - strandat 2221.683 kb on - strandat 2222.711 kb on - strand, within Pf6N2E2_1874at 2222.892 kb on + strandat 2222.929 kb on - strandat 2223.001 kb on - strandat 2223.137 kb on + strandat 2223.138 kb on - strandat 2223.160 kb on - strandat 2223.304 kb on + strand, within Pf6N2E2_1875at 2223.573 kb on - strand, within Pf6N2E2_1875at 2223.759 kb on + strand, within Pf6N2E2_1875

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Per-strain Table

Position Strand Gene LocusTag Fraction 50% P. fluorescens FW300-N2E2 spent media from growth in 20 mM putrescine
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2,220,643 - -0.3
2,220,780 - +0.7
2,220,934 - +0.7
2,220,956 - +0.1
2,220,956 - +0.2
2,220,956 - +1.0
2,220,991 - -0.3
2,221,068 - -0.3
2,221,132 - +0.1
2,221,190 + +1.2
2,221,191 - -0.3
2,221,669 - +1.2
2,221,683 - +1.7
2,222,711 - Pf6N2E2_1874 0.81 -1.9
2,222,892 + -0.1
2,222,929 - -2.3
2,223,001 - +0.3
2,223,137 + +0.7
2,223,138 - +0.5
2,223,160 - +0.3
2,223,304 + Pf6N2E2_1875 0.20 -0.3
2,223,573 - Pf6N2E2_1875 0.40 +1.3
2,223,759 + Pf6N2E2_1875 0.54 -0.9

Or see this region's nucleotide sequence