Experiment: 50% P. fluorescens FW300-N2E2 spent media from growth in 20 mM putrescine
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt Pf6N2E2_1684 and Pf6N2E2_1685 are separated by 64 nucleotides Pf6N2E2_1685 and Pf6N2E2_1686 overlap by 4 nucleotides
Pf6N2E2_1684 - Ferrichrome-iron receptor, at 1,991,867 to 1,994,230
_1684
Pf6N2E2_1685 - Vitamin B12 ABC transporter, B12-binding component BtuF, at 1,994,295 to 1,995,482
_1685
Pf6N2E2_1686 - Iron(III) dicitrate transport system permease protein FecD (TC 3.A.1.14.1), at 1,995,479 to 1,996,540
_1686
Position (kb) 1994
1995
1996 Strain fitness (log2 ratio) -2
-1
0
1
2 at 1993.314 kb on - strand, within Pf6N2E2_1684 at 1993.314 kb on - strand, within Pf6N2E2_1684 at 1993.314 kb on - strand, within Pf6N2E2_1684 at 1993.478 kb on + strand, within Pf6N2E2_1684 at 1993.478 kb on + strand, within Pf6N2E2_1684 at 1993.479 kb on - strand, within Pf6N2E2_1684 at 1993.479 kb on - strand, within Pf6N2E2_1684 at 1993.506 kb on - strand, within Pf6N2E2_1684 at 1993.572 kb on - strand, within Pf6N2E2_1684 at 1993.574 kb on + strand, within Pf6N2E2_1684 at 1993.574 kb on + strand, within Pf6N2E2_1684 at 1993.575 kb on - strand, within Pf6N2E2_1684 at 1993.575 kb on - strand, within Pf6N2E2_1684 at 1993.575 kb on - strand, within Pf6N2E2_1684 at 1993.580 kb on + strand, within Pf6N2E2_1684 at 1993.580 kb on + strand, within Pf6N2E2_1684 at 1993.652 kb on + strand, within Pf6N2E2_1684 at 1993.652 kb on + strand, within Pf6N2E2_1684 at 1993.652 kb on + strand, within Pf6N2E2_1684 at 1993.653 kb on - strand, within Pf6N2E2_1684 at 1993.692 kb on - strand, within Pf6N2E2_1684 at 1993.692 kb on - strand, within Pf6N2E2_1684 at 1993.857 kb on + strand, within Pf6N2E2_1684 at 1993.907 kb on - strand, within Pf6N2E2_1684 at 1994.168 kb on + strand at 1994.169 kb on - strand at 1994.585 kb on + strand, within Pf6N2E2_1685 at 1994.590 kb on - strand, within Pf6N2E2_1685 at 1994.745 kb on + strand, within Pf6N2E2_1685 at 1994.784 kb on + strand, within Pf6N2E2_1685 at 1994.785 kb on - strand, within Pf6N2E2_1685 at 1994.785 kb on - strand, within Pf6N2E2_1685 at 1994.976 kb on + strand at 1995.232 kb on - strand, within Pf6N2E2_1685 at 1995.306 kb on + strand, within Pf6N2E2_1685 at 1995.307 kb on - strand, within Pf6N2E2_1685 at 1995.307 kb on - strand, within Pf6N2E2_1685 at 1995.433 kb on - strand at 1995.433 kb on - strand
Per-strain Table
Position Strand Gene LocusTag Fraction 50% P. fluorescens FW300-N2E2 spent media from growth in 20 mM putrescine remove 1,993,314 - Pf6N2E2_1684 0.61 -1.0 1,993,314 - Pf6N2E2_1684 0.61 -0.1 1,993,314 - Pf6N2E2_1684 0.61 +0.0 1,993,478 + Pf6N2E2_1684 0.68 +0.6 1,993,478 + Pf6N2E2_1684 0.68 -1.0 1,993,479 - Pf6N2E2_1684 0.68 +0.1 1,993,479 - Pf6N2E2_1684 0.68 -0.4 1,993,506 - Pf6N2E2_1684 0.69 +0.2 1,993,572 - Pf6N2E2_1684 0.72 -0.4 1,993,574 + Pf6N2E2_1684 0.72 +0.9 1,993,574 + Pf6N2E2_1684 0.72 +0.6 1,993,575 - Pf6N2E2_1684 0.72 +0.9 1,993,575 - Pf6N2E2_1684 0.72 -0.4 1,993,575 - Pf6N2E2_1684 0.72 -0.9 1,993,580 + Pf6N2E2_1684 0.72 +0.4 1,993,580 + Pf6N2E2_1684 0.72 +0.1 1,993,652 + Pf6N2E2_1684 0.76 +2.0 1,993,652 + Pf6N2E2_1684 0.76 +0.1 1,993,652 + Pf6N2E2_1684 0.76 -1.2 1,993,653 - Pf6N2E2_1684 0.76 -1.4 1,993,692 - Pf6N2E2_1684 0.77 +0.1 1,993,692 - Pf6N2E2_1684 0.77 -1.6 1,993,857 + Pf6N2E2_1684 0.84 +0.6 1,993,907 - Pf6N2E2_1684 0.86 +1.8 1,994,168 + +1.0 1,994,169 - -0.4 1,994,585 + Pf6N2E2_1685 0.24 +1.1 1,994,590 - Pf6N2E2_1685 0.25 -0.8 1,994,745 + Pf6N2E2_1685 0.38 -0.3 1,994,784 + Pf6N2E2_1685 0.41 -0.8 1,994,785 - Pf6N2E2_1685 0.41 -1.8 1,994,785 - Pf6N2E2_1685 0.41 -0.9 1,994,976 + -0.4 1,995,232 - Pf6N2E2_1685 0.79 -0.1 1,995,306 + Pf6N2E2_1685 0.85 -0.4 1,995,307 - Pf6N2E2_1685 0.85 -0.3 1,995,307 - Pf6N2E2_1685 0.85 -1.2 1,995,433 - +1.3 1,995,433 - +2.3
Or see this region's nucleotide sequence