Experiment: 50% P. fluorescens FW300-N2E2 spent media from growth in 20 mM putrescine
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt Pf6N2E2_162 and Pf6N2E2_163 overlap by 4 nucleotides Pf6N2E2_163 and Pf6N2E2_164 are separated by 3 nucleotides Pf6N2E2_164 and Pf6N2E2_165 are separated by 23 nucleotides Pf6N2E2_165 and Pf6N2E2_166 overlap by 4 nucleotides
Pf6N2E2_162 - Ribose ABC transport system, ATP-binding protein RbsA (TC 3.A.1.2.1), at 215,296 to 216,849
_162
Pf6N2E2_163 - Ribose ABC transport system, permease protein RbsC (TC 3.A.1.2.1), at 216,846 to 217,823
_163
Pf6N2E2_164 - Ribose operon repressor, at 217,827 to 218,846
_164
Pf6N2E2_165 - Ribokinase (EC 2.7.1.15), at 218,870 to 219,784
_165
Pf6N2E2_166 - Ribose ABC transport system, high affinity permease RbsD (TC 3.A.1.2.1), at 219,781 to 220,185
_166
Position (kb) 217
218
219 Strain fitness (log2 ratio) -2
-1
0
1 at 216.888 kb on + strand at 216.889 kb on - strand at 216.903 kb on + strand at 216.904 kb on - strand at 216.975 kb on + strand, within Pf6N2E2_163 at 216.975 kb on + strand, within Pf6N2E2_163 at 216.990 kb on - strand, within Pf6N2E2_163 at 217.302 kb on + strand, within Pf6N2E2_163 at 217.302 kb on + strand, within Pf6N2E2_163 at 217.303 kb on - strand, within Pf6N2E2_163 at 217.303 kb on - strand, within Pf6N2E2_163 at 217.492 kb on - strand, within Pf6N2E2_163 at 217.505 kb on - strand, within Pf6N2E2_163 at 217.631 kb on + strand, within Pf6N2E2_163 at 217.631 kb on + strand, within Pf6N2E2_163 at 217.791 kb on + strand at 217.792 kb on - strand at 217.847 kb on - strand at 217.959 kb on + strand, within Pf6N2E2_164 at 217.959 kb on + strand, within Pf6N2E2_164 at 217.960 kb on - strand, within Pf6N2E2_164 at 218.043 kb on + strand, within Pf6N2E2_164 at 218.044 kb on - strand, within Pf6N2E2_164 at 218.044 kb on - strand, within Pf6N2E2_164 at 218.076 kb on + strand, within Pf6N2E2_164 at 218.076 kb on + strand, within Pf6N2E2_164 at 218.076 kb on + strand, within Pf6N2E2_164 at 218.145 kb on + strand, within Pf6N2E2_164 at 218.145 kb on + strand, within Pf6N2E2_164 at 218.291 kb on - strand, within Pf6N2E2_164 at 218.310 kb on + strand, within Pf6N2E2_164 at 218.310 kb on + strand, within Pf6N2E2_164 at 218.311 kb on - strand, within Pf6N2E2_164 at 218.319 kb on + strand, within Pf6N2E2_164 at 218.320 kb on - strand, within Pf6N2E2_164 at 218.387 kb on + strand, within Pf6N2E2_164 at 218.524 kb on + strand, within Pf6N2E2_164 at 218.524 kb on + strand, within Pf6N2E2_164 at 218.525 kb on - strand, within Pf6N2E2_164 at 218.525 kb on - strand, within Pf6N2E2_164 at 218.662 kb on - strand, within Pf6N2E2_164 at 218.662 kb on - strand, within Pf6N2E2_164 at 218.789 kb on + strand at 218.789 kb on + strand at 218.789 kb on + strand at 218.789 kb on + strand at 218.789 kb on + strand at 218.789 kb on + strand at 218.789 kb on + strand at 218.789 kb on + strand at 218.789 kb on + strand at 218.789 kb on + strand at 218.790 kb on - strand at 218.790 kb on - strand at 218.790 kb on - strand at 218.861 kb on + strand at 218.861 kb on + strand at 218.869 kb on + strand at 218.869 kb on + strand at 218.869 kb on + strand at 218.869 kb on + strand at 218.869 kb on + strand at 218.869 kb on + strand at 218.869 kb on + strand at 218.869 kb on + strand at 218.869 kb on + strand at 218.869 kb on + strand at 218.870 kb on - strand at 218.870 kb on - strand at 218.870 kb on - strand at 218.870 kb on - strand at 218.870 kb on - strand at 218.870 kb on - strand at 218.870 kb on - strand at 218.870 kb on - strand at 218.883 kb on + strand at 218.883 kb on + strand at 218.883 kb on + strand at 218.883 kb on + strand at 218.884 kb on - strand at 218.890 kb on - strand at 218.892 kb on + strand at 218.892 kb on + strand at 218.892 kb on + strand at 219.377 kb on - strand, within Pf6N2E2_165 at 219.395 kb on + strand, within Pf6N2E2_165 at 219.421 kb on + strand, within Pf6N2E2_165 at 219.421 kb on + strand, within Pf6N2E2_165 at 219.421 kb on + strand, within Pf6N2E2_165 at 219.421 kb on + strand, within Pf6N2E2_165 at 219.765 kb on + strand at 219.766 kb on - strand at 219.766 kb on - strand at 219.766 kb on - strand
Per-strain Table
Position Strand Gene LocusTag Fraction 50% P. fluorescens FW300-N2E2 spent media from growth in 20 mM putrescine remove 216,888 + +1.4 216,889 - -0.2 216,903 + +0.4 216,904 - -0.7 216,975 + Pf6N2E2_163 0.13 -0.2 216,975 + Pf6N2E2_163 0.13 +1.3 216,990 - Pf6N2E2_163 0.15 -0.2 217,302 + Pf6N2E2_163 0.47 -1.7 217,302 + Pf6N2E2_163 0.47 -0.5 217,303 - Pf6N2E2_163 0.47 -0.9 217,303 - Pf6N2E2_163 0.47 +1.2 217,492 - Pf6N2E2_163 0.66 -0.2 217,505 - Pf6N2E2_163 0.67 -0.7 217,631 + Pf6N2E2_163 0.80 -1.0 217,631 + Pf6N2E2_163 0.80 -0.3 217,791 + +0.5 217,792 - +0.1 217,847 - -0.2 217,959 + Pf6N2E2_164 0.13 -0.3 217,959 + Pf6N2E2_164 0.13 -1.2 217,960 - Pf6N2E2_164 0.13 -0.4 218,043 + Pf6N2E2_164 0.21 -0.8 218,044 - Pf6N2E2_164 0.21 -0.2 218,044 - Pf6N2E2_164 0.21 -0.7 218,076 + Pf6N2E2_164 0.24 -0.2 218,076 + Pf6N2E2_164 0.24 -0.7 218,076 + Pf6N2E2_164 0.24 -0.7 218,145 + Pf6N2E2_164 0.31 -0.5 218,145 + Pf6N2E2_164 0.31 -0.2 218,291 - Pf6N2E2_164 0.45 -0.7 218,310 + Pf6N2E2_164 0.47 -0.2 218,310 + Pf6N2E2_164 0.47 -0.2 218,311 - Pf6N2E2_164 0.47 -1.7 218,319 + Pf6N2E2_164 0.48 +0.8 218,320 - Pf6N2E2_164 0.48 +0.6 218,387 + Pf6N2E2_164 0.55 -2.3 218,524 + Pf6N2E2_164 0.68 -0.7 218,524 + Pf6N2E2_164 0.68 -0.2 218,525 - Pf6N2E2_164 0.68 -2.0 218,525 - Pf6N2E2_164 0.68 -0.2 218,662 - Pf6N2E2_164 0.82 -1.1 218,662 - Pf6N2E2_164 0.82 -0.6 218,789 + -2.7 218,789 + -2.0 218,789 + -0.2 218,789 + -0.4 218,789 + -0.2 218,789 + -0.9 218,789 + -0.2 218,789 + +0.8 218,789 + +0.4 218,789 + -0.8 218,790 - -0.2 218,790 - -0.6 218,790 - -0.9 218,861 + -1.7 218,861 + -1.7 218,869 + +1.3 218,869 + +1.4 218,869 + +0.1 218,869 + +1.4 218,869 + -0.6 218,869 + +0.1 218,869 + -0.5 218,869 + +0.3 218,869 + +0.4 218,869 + -0.2 218,870 - +0.5 218,870 - -0.2 218,870 - -0.2 218,870 - -0.2 218,870 - +0.2 218,870 - +1.0 218,870 - -0.2 218,870 - +0.9 218,883 + -0.0 218,883 + -0.2 218,883 + +0.8 218,883 + +0.3 218,884 - -0.2 218,890 - +0.1 218,892 + +0.7 218,892 + +0.0 218,892 + +0.3 219,377 - Pf6N2E2_165 0.55 +1.3 219,395 + Pf6N2E2_165 0.57 -0.5 219,421 + Pf6N2E2_165 0.60 +0.6 219,421 + Pf6N2E2_165 0.60 +0.1 219,421 + Pf6N2E2_165 0.60 -0.6 219,421 + Pf6N2E2_165 0.60 +0.2 219,765 + +0.3 219,766 - +1.0 219,766 - -0.0 219,766 - +0.8
Or see this region's nucleotide sequence