Experiment: 50% P. fluorescens FW300-N2E2 spent media from growth in 20 mM putrescine
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt Pf6N2E2_1532 and Pf6N2E2_1533 are separated by 28 nucleotides Pf6N2E2_1533 and Pf6N2E2_1534 are separated by 19 nucleotides Pf6N2E2_1534 and Pf6N2E2_1535 overlap by 4 nucleotides Pf6N2E2_1535 and Pf6N2E2_1536 are separated by 13 nucleotides Pf6N2E2_1536 and Pf6N2E2_1537 are separated by 95 nucleotides
Pf6N2E2_1532 - FIG003847: Oxidoreductase (flavoprotein), at 1,792,255 to 1,793,925
_1532
Pf6N2E2_1533 - membrane protein, putative, at 1,793,954 to 1,794,757
_1533
Pf6N2E2_1534 - Probable transmembrane protein, at 1,794,777 to 1,795,235
_1534
Pf6N2E2_1535 - GENE II AND X PROTEINS, at 1,795,232 to 1,795,657
_1535
Pf6N2E2_1536 - Permeases of the major facilitator superfamily, at 1,795,671 to 1,795,859
_1536
Pf6N2E2_1537 - monooxygenase, putative, at 1,795,955 to 1,797,046
_1537
Position (kb)
1794
1795
1796 Strain fitness (log2 ratio)
-2
-1
0
1
2 at 1793.808 kb on + strand at 1793.838 kb on + strand at 1793.838 kb on + strand at 1793.838 kb on + strand at 1793.838 kb on + strand at 1793.838 kb on + strand at 1793.839 kb on - strand at 1793.839 kb on - strand at 1793.839 kb on - strand at 1793.863 kb on + strand at 1793.921 kb on + strand at 1793.921 kb on + strand at 1793.921 kb on + strand at 1793.922 kb on - strand at 1793.922 kb on - strand at 1794.024 kb on + strand at 1794.155 kb on + strand, within Pf6N2E2_1533 at 1794.272 kb on + strand, within Pf6N2E2_1533 at 1794.272 kb on + strand, within Pf6N2E2_1533 at 1794.272 kb on + strand, within Pf6N2E2_1533 at 1794.272 kb on + strand, within Pf6N2E2_1533 at 1794.272 kb on + strand, within Pf6N2E2_1533 at 1794.273 kb on - strand at 1794.273 kb on - strand, within Pf6N2E2_1533 at 1794.273 kb on - strand, within Pf6N2E2_1533 at 1794.273 kb on - strand, within Pf6N2E2_1533 at 1794.615 kb on + strand, within Pf6N2E2_1533 at 1794.686 kb on + strand at 1794.687 kb on - strand at 1794.741 kb on + strand at 1794.741 kb on + strand at 1794.772 kb on + strand at 1794.772 kb on + strand at 1794.825 kb on - strand, within Pf6N2E2_1534 at 1794.825 kb on - strand, within Pf6N2E2_1534 at 1795.204 kb on + strand at 1795.204 kb on + strand at 1795.204 kb on + strand at 1795.205 kb on - strand at 1795.709 kb on + strand, within Pf6N2E2_1536 at 1795.709 kb on + strand, within Pf6N2E2_1536 at 1795.710 kb on - strand, within Pf6N2E2_1536 at 1795.710 kb on - strand, within Pf6N2E2_1536 at 1795.730 kb on + strand, within Pf6N2E2_1536 at 1795.730 kb on + strand, within Pf6N2E2_1536 at 1795.732 kb on + strand, within Pf6N2E2_1536 at 1795.732 kb on + strand, within Pf6N2E2_1536 at 1795.733 kb on - strand, within Pf6N2E2_1536 at 1795.799 kb on + strand, within Pf6N2E2_1536 at 1795.821 kb on + strand, within Pf6N2E2_1536 at 1795.821 kb on + strand, within Pf6N2E2_1536 at 1795.821 kb on + strand, within Pf6N2E2_1536 at 1795.864 kb on + strand at 1795.865 kb on - strand at 1795.986 kb on + strand at 1795.986 kb on + strand at 1795.986 kb on + strand at 1795.987 kb on - strand at 1795.987 kb on - strand at 1795.987 kb on - strand at 1796.028 kb on - strand at 1796.050 kb on - strand at 1796.223 kb on - strand, within Pf6N2E2_1537 at 1796.223 kb on - strand, within Pf6N2E2_1537
Per-strain Table
Position Strand Gene LocusTag Fraction 50% P. fluorescens FW300-N2E2 spent media from growth in 20 mM putrescine remove 1,793,808 + +1.5 1,793,838 + +1.2 1,793,838 + +0.3 1,793,838 + -0.5 1,793,838 + +0.5 1,793,838 + +0.1 1,793,839 - +1.2 1,793,839 - -0.5 1,793,839 - +1.1 1,793,863 + +1.5 1,793,921 + +0.4 1,793,921 + +0.7 1,793,921 + +0.1 1,793,922 - -0.5 1,793,922 - +0.5 1,794,024 + -0.5 1,794,155 + Pf6N2E2_1533 0.25 +0.6 1,794,272 + Pf6N2E2_1533 0.40 +2.3 1,794,272 + Pf6N2E2_1533 0.40 -0.4 1,794,272 + Pf6N2E2_1533 0.40 +0.7 1,794,272 + Pf6N2E2_1533 0.40 +1.4 1,794,272 + Pf6N2E2_1533 0.40 +0.1 1,794,273 - -0.5 1,794,273 - Pf6N2E2_1533 0.40 +0.1 1,794,273 - Pf6N2E2_1533 0.40 +1.8 1,794,273 - Pf6N2E2_1533 0.40 +0.8 1,794,615 + Pf6N2E2_1533 0.82 +0.3 1,794,686 + -0.5 1,794,687 - +1.1 1,794,741 + +0.5 1,794,741 + +0.8 1,794,772 + -1.5 1,794,772 + -0.1 1,794,825 - Pf6N2E2_1534 0.10 -1.8 1,794,825 - Pf6N2E2_1534 0.10 -0.2 1,795,204 + +1.5 1,795,204 + +0.5 1,795,204 + -0.0 1,795,205 - +0.9 1,795,709 + Pf6N2E2_1536 0.20 +2.5 1,795,709 + Pf6N2E2_1536 0.20 +0.6 1,795,710 - Pf6N2E2_1536 0.21 +1.1 1,795,710 - Pf6N2E2_1536 0.21 +0.1 1,795,730 + Pf6N2E2_1536 0.31 -0.5 1,795,730 + Pf6N2E2_1536 0.31 -0.2 1,795,732 + Pf6N2E2_1536 0.32 +2.3 1,795,732 + Pf6N2E2_1536 0.32 -0.3 1,795,733 - Pf6N2E2_1536 0.33 -1.5 1,795,799 + Pf6N2E2_1536 0.68 -0.5 1,795,821 + Pf6N2E2_1536 0.79 +0.3 1,795,821 + Pf6N2E2_1536 0.79 +1.1 1,795,821 + Pf6N2E2_1536 0.79 +1.1 1,795,864 + +0.0 1,795,865 - +1.1 1,795,986 + -0.2 1,795,986 + -0.5 1,795,986 + -1.0 1,795,987 - -0.5 1,795,987 - -0.5 1,795,987 - +0.1 1,796,028 - +0.8 1,796,050 - -0.5 1,796,223 - Pf6N2E2_1537 0.25 +0.1 1,796,223 - Pf6N2E2_1537 0.25 -0.5
Or see this region's nucleotide sequence