Experiment: 50% P. fluorescens FW300-N2E2 spent media from growth in 20 mM putrescine
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt Pf6N2E2_145 and Pf6N2E2_146 are separated by 235 nucleotides Pf6N2E2_146 and Pf6N2E2_147 are separated by 4 nucleotides
Pf6N2E2_145 - putative exported protein, at 197,183 to 200,836
_145
Pf6N2E2_146 - Transcriptional regulator, MarR family, at 201,072 to 201,554
_146
Pf6N2E2_147 - Outer membrane component of tripartite multidrug resistance system, at 201,559 to 203,013
_147
Position (kb) 201
202 Strain fitness (log2 ratio) -2
-1
0
1 at 200.363 kb on + strand, within Pf6N2E2_145 at 200.363 kb on + strand, within Pf6N2E2_145 at 200.492 kb on + strand at 200.493 kb on - strand at 200.608 kb on + strand at 200.754 kb on - strand at 200.774 kb on + strand at 200.774 kb on + strand at 200.775 kb on - strand at 200.775 kb on - strand at 200.819 kb on + strand at 200.819 kb on + strand at 200.819 kb on + strand at 200.820 kb on - strand at 200.820 kb on - strand at 200.820 kb on - strand at 200.820 kb on - strand at 200.820 kb on - strand at 200.820 kb on - strand at 200.834 kb on + strand at 200.835 kb on - strand at 200.835 kb on - strand at 200.881 kb on - strand at 200.959 kb on + strand at 200.959 kb on + strand at 200.960 kb on - strand at 200.960 kb on - strand at 200.960 kb on - strand at 200.977 kb on + strand at 200.993 kb on - strand at 200.996 kb on + strand at 200.997 kb on - strand at 200.997 kb on - strand at 201.018 kb on + strand at 201.039 kb on - strand at 201.039 kb on - strand at 201.039 kb on - strand at 201.039 kb on - strand at 201.052 kb on - strand at 201.167 kb on + strand, within Pf6N2E2_146 at 201.167 kb on + strand, within Pf6N2E2_146 at 201.552 kb on + strand at 201.552 kb on + strand at 201.553 kb on - strand at 201.553 kb on - strand at 201.616 kb on + strand at 201.838 kb on + strand, within Pf6N2E2_147 at 201.839 kb on - strand, within Pf6N2E2_147 at 201.940 kb on + strand, within Pf6N2E2_147 at 201.940 kb on + strand, within Pf6N2E2_147 at 201.940 kb on + strand, within Pf6N2E2_147 at 201.941 kb on - strand, within Pf6N2E2_147 at 201.941 kb on - strand, within Pf6N2E2_147 at 201.941 kb on - strand, within Pf6N2E2_147 at 201.960 kb on - strand, within Pf6N2E2_147 at 201.960 kb on - strand, within Pf6N2E2_147 at 201.976 kb on + strand, within Pf6N2E2_147 at 201.976 kb on + strand, within Pf6N2E2_147 at 201.976 kb on + strand, within Pf6N2E2_147 at 201.976 kb on + strand, within Pf6N2E2_147 at 201.977 kb on - strand, within Pf6N2E2_147 at 201.977 kb on - strand, within Pf6N2E2_147 at 202.100 kb on - strand, within Pf6N2E2_147 at 202.125 kb on + strand, within Pf6N2E2_147 at 202.125 kb on + strand, within Pf6N2E2_147 at 202.125 kb on + strand, within Pf6N2E2_147 at 202.125 kb on + strand, within Pf6N2E2_147 at 202.126 kb on - strand, within Pf6N2E2_147 at 202.126 kb on - strand, within Pf6N2E2_147 at 202.126 kb on - strand, within Pf6N2E2_147 at 202.126 kb on - strand, within Pf6N2E2_147 at 202.126 kb on - strand, within Pf6N2E2_147 at 202.126 kb on - strand, within Pf6N2E2_147 at 202.126 kb on - strand, within Pf6N2E2_147 at 202.129 kb on + strand, within Pf6N2E2_147 at 202.129 kb on + strand, within Pf6N2E2_147 at 202.129 kb on + strand, within Pf6N2E2_147 at 202.129 kb on + strand, within Pf6N2E2_147 at 202.130 kb on - strand, within Pf6N2E2_147 at 202.217 kb on - strand, within Pf6N2E2_147 at 202.498 kb on + strand, within Pf6N2E2_147
Per-strain Table
Position Strand Gene LocusTag Fraction 50% P. fluorescens FW300-N2E2 spent media from growth in 20 mM putrescine remove 200,363 + Pf6N2E2_145 0.87 +1.0 200,363 + Pf6N2E2_145 0.87 +0.8 200,492 + +1.0 200,493 - +0.7 200,608 + +0.6 200,754 - +0.8 200,774 + +0.9 200,774 + +1.5 200,775 - +0.2 200,775 - +0.8 200,819 + +0.1 200,819 + +0.7 200,819 + +0.8 200,820 - +0.8 200,820 - +0.7 200,820 - +0.0 200,820 - +0.1 200,820 - +0.5 200,820 - +1.1 200,834 + -1.0 200,835 - +0.3 200,835 - -0.1 200,881 - +0.6 200,959 + -0.1 200,959 + -1.7 200,960 - +0.3 200,960 - -0.5 200,960 - +0.1 200,977 + -0.7 200,993 - +0.3 200,996 + -0.1 200,997 - -0.1 200,997 - +0.9 201,018 + +0.4 201,039 - +0.4 201,039 - -0.6 201,039 - -0.1 201,039 - +0.4 201,052 - -0.4 201,167 + Pf6N2E2_146 0.20 -0.7 201,167 + Pf6N2E2_146 0.20 -0.5 201,552 + -0.4 201,552 + -0.1 201,553 - +1.4 201,553 - -0.1 201,616 + -0.6 201,838 + Pf6N2E2_147 0.19 +0.4 201,839 - Pf6N2E2_147 0.19 -0.1 201,940 + Pf6N2E2_147 0.26 -0.1 201,940 + Pf6N2E2_147 0.26 -2.1 201,940 + Pf6N2E2_147 0.26 -0.1 201,941 - Pf6N2E2_147 0.26 +0.0 201,941 - Pf6N2E2_147 0.26 -1.0 201,941 - Pf6N2E2_147 0.26 -0.1 201,960 - Pf6N2E2_147 0.28 -0.1 201,960 - Pf6N2E2_147 0.28 -0.0 201,976 + Pf6N2E2_147 0.29 -1.1 201,976 + Pf6N2E2_147 0.29 +0.2 201,976 + Pf6N2E2_147 0.29 +0.2 201,976 + Pf6N2E2_147 0.29 -1.1 201,977 - Pf6N2E2_147 0.29 +0.2 201,977 - Pf6N2E2_147 0.29 -0.9 202,100 - Pf6N2E2_147 0.37 +1.1 202,125 + Pf6N2E2_147 0.39 -2.1 202,125 + Pf6N2E2_147 0.39 +0.0 202,125 + Pf6N2E2_147 0.39 +0.4 202,125 + Pf6N2E2_147 0.39 +0.4 202,126 - Pf6N2E2_147 0.39 -0.5 202,126 - Pf6N2E2_147 0.39 -0.4 202,126 - Pf6N2E2_147 0.39 -2.1 202,126 - Pf6N2E2_147 0.39 +0.7 202,126 - Pf6N2E2_147 0.39 -0.1 202,126 - Pf6N2E2_147 0.39 -0.7 202,126 - Pf6N2E2_147 0.39 -0.5 202,129 + Pf6N2E2_147 0.39 +0.6 202,129 + Pf6N2E2_147 0.39 -0.0 202,129 + Pf6N2E2_147 0.39 +0.5 202,129 + Pf6N2E2_147 0.39 -0.0 202,130 - Pf6N2E2_147 0.39 +1.7 202,217 - Pf6N2E2_147 0.45 -0.1 202,498 + Pf6N2E2_147 0.65 +0.9
Or see this region's nucleotide sequence