Experiment: Glucose (C) and L-Glutamine (N); with TAPS
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt PFLU_RS17995 and PFLU_RS18000 overlap by 4 nucleotides PFLU_RS18000 and PFLU_RS18005 are separated by 30 nucleotides PFLU_RS18005 and PFLU_RS18010 overlap by 4 nucleotides
PFLU_RS17995: PFLU_RS17995 - LLM class flavin-dependent oxidoreductase, at 4,083,291 to 4,084,655
_RS17995
PFLU_RS18000: PFLU_RS18000 - AhpC/TSA family protein, at 4,084,652 to 4,085,335
_RS18000
PFLU_RS18005: PFLU_RS18005 - acyl-CoA/acyl-ACP dehydrogenase, at 4,085,366 to 4,086,544
_RS18005
PFLU_RS18010: PFLU_RS18010 - dihydrofolate reductase, at 4,086,541 to 4,087,485
_RS18010
Position (kb)
4085
4086
4087 Strain fitness (log2 ratio)
-3
-2
-1
0
1
2 at 4084.498 kb on - strand, within PFLU_RS17995 at 4084.555 kb on - strand at 4084.555 kb on - strand at 4084.555 kb on - strand at 4084.595 kb on - strand at 4084.781 kb on + strand, within PFLU_RS18000 at 4084.781 kb on + strand, within PFLU_RS18000 at 4084.782 kb on - strand, within PFLU_RS18000 at 4084.782 kb on - strand, within PFLU_RS18000 at 4084.934 kb on + strand, within PFLU_RS18000 at 4085.330 kb on - strand at 4085.356 kb on + strand at 4085.356 kb on + strand at 4085.357 kb on - strand at 4085.468 kb on + strand at 4085.569 kb on + strand, within PFLU_RS18005 at 4085.570 kb on - strand, within PFLU_RS18005 at 4085.649 kb on - strand, within PFLU_RS18005 at 4085.852 kb on + strand, within PFLU_RS18005 at 4085.852 kb on + strand, within PFLU_RS18005 at 4086.450 kb on + strand at 4086.451 kb on - strand at 4086.451 kb on - strand at 4086.451 kb on - strand at 4086.451 kb on - strand at 4086.580 kb on + strand at 4086.581 kb on - strand at 4086.581 kb on - strand at 4086.581 kb on - strand at 4086.581 kb on - strand at 4086.686 kb on + strand, within PFLU_RS18010 at 4086.687 kb on - strand, within PFLU_RS18010 at 4086.716 kb on - strand, within PFLU_RS18010 at 4086.716 kb on - strand, within PFLU_RS18010 at 4087.170 kb on + strand, within PFLU_RS18010 at 4087.170 kb on + strand, within PFLU_RS18010 at 4087.170 kb on + strand, within PFLU_RS18010 at 4087.171 kb on - strand, within PFLU_RS18010 at 4087.225 kb on - strand, within PFLU_RS18010 at 4087.307 kb on + strand, within PFLU_RS18010 at 4087.345 kb on + strand, within PFLU_RS18010 at 4087.345 kb on + strand, within PFLU_RS18010 at 4087.345 kb on + strand, within PFLU_RS18010 at 4087.345 kb on + strand, within PFLU_RS18010 at 4087.346 kb on - strand, within PFLU_RS18010 at 4087.444 kb on + strand at 4087.444 kb on + strand at 4087.445 kb on - strand at 4087.445 kb on - strand at 4087.445 kb on - strand at 4087.445 kb on - strand at 4087.446 kb on + strand at 4087.447 kb on - strand at 4087.447 kb on - strand at 4087.447 kb on - strand at 4087.455 kb on + strand at 4087.455 kb on + strand at 4087.456 kb on - strand at 4087.483 kb on + strand
Per-strain Table
Position Strand Gene LocusTag Fraction Glucose (C) and L-Glutamine (N); with TAPS remove 4,084,498 - PFLU_RS17995 0.88 +0.3 4,084,555 - +1.8 4,084,555 - -1.1 4,084,555 - +0.2 4,084,595 - +0.2 4,084,781 + PFLU_RS18000 0.19 -1.0 4,084,781 + PFLU_RS18000 0.19 +0.9 4,084,782 - PFLU_RS18000 0.19 -0.5 4,084,782 - PFLU_RS18000 0.19 -0.5 4,084,934 + PFLU_RS18000 0.41 +0.7 4,085,330 - -0.4 4,085,356 + -0.1 4,085,356 + +0.5 4,085,357 - +0.7 4,085,468 + -0.1 4,085,569 + PFLU_RS18005 0.17 -0.4 4,085,570 - PFLU_RS18005 0.17 +0.1 4,085,649 - PFLU_RS18005 0.24 -2.6 4,085,852 + PFLU_RS18005 0.41 -1.3 4,085,852 + PFLU_RS18005 0.41 -0.5 4,086,450 + -0.7 4,086,451 - +0.2 4,086,451 - +1.5 4,086,451 - -2.4 4,086,451 - -2.9 4,086,580 + +2.1 4,086,581 - +0.1 4,086,581 - +0.1 4,086,581 - +1.7 4,086,581 - +0.5 4,086,686 + PFLU_RS18010 0.15 +0.0 4,086,687 - PFLU_RS18010 0.15 +1.6 4,086,716 - PFLU_RS18010 0.19 -0.5 4,086,716 - PFLU_RS18010 0.19 +1.2 4,087,170 + PFLU_RS18010 0.67 -0.7 4,087,170 + PFLU_RS18010 0.67 +0.5 4,087,170 + PFLU_RS18010 0.67 -0.8 4,087,171 - PFLU_RS18010 0.67 -1.0 4,087,225 - PFLU_RS18010 0.72 +2.5 4,087,307 + PFLU_RS18010 0.81 -1.0 4,087,345 + PFLU_RS18010 0.85 -2.4 4,087,345 + PFLU_RS18010 0.85 -1.0 4,087,345 + PFLU_RS18010 0.85 -1.9 4,087,345 + PFLU_RS18010 0.85 +1.0 4,087,346 - PFLU_RS18010 0.85 -1.1 4,087,444 + -0.2 4,087,444 + -1.7 4,087,445 - +0.7 4,087,445 - -2.1 4,087,445 - +0.7 4,087,445 - +0.1 4,087,446 + -2.0 4,087,447 - +1.8 4,087,447 - -0.5 4,087,447 - +1.5 4,087,455 + -2.0 4,087,455 + -0.2 4,087,456 - -1.4 4,087,483 + +0.3
Or see this region's nucleotide sequence