Experiment: MoLS4 with Cytosine 20mM (N)
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt DVU2116 and DVU2117 are separated by 220 nucleotides DVU2117 and DVU2118 are separated by 9 nucleotides DVU2118 and cpaC overlap by 4 nucleotides
DVU2116: DVU2116 - pilin, putative (TIGR), at 2,212,101 to 2,212,274
DVU2116
DVU2117: DVU2117 - membrane protein, putative (TIGR), at 2,212,495 to 2,213,061
DVU2117
DVU2118: DVU2118 - conserved hypothetical protein (TIGR), at 2,213,071 to 2,213,889
DVU2118
DVU2119: cpaC - type II/III secretion system protein (TIGR), at 2,213,886 to 2,215,340
cpaC
Position (kb)
2213
2214 Strain fitness (log2 ratio)
-3
-2
-1
0
1
2 at 2212.121 kb on + strand, within DVU2116 at 2212.197 kb on + strand, within DVU2116 at 2212.213 kb on + strand, within DVU2116 at 2212.216 kb on - strand, within DVU2116 at 2212.221 kb on - strand, within DVU2116 at 2212.269 kb on + strand at 2212.283 kb on - strand at 2212.301 kb on + strand at 2212.310 kb on + strand at 2212.339 kb on + strand at 2212.347 kb on + strand at 2212.347 kb on + strand at 2212.362 kb on - strand at 2212.412 kb on + strand at 2212.506 kb on + strand at 2212.571 kb on + strand, within DVU2117 at 2212.617 kb on + strand, within DVU2117 at 2212.624 kb on + strand, within DVU2117 at 2212.672 kb on - strand, within DVU2117 at 2212.698 kb on - strand, within DVU2117 at 2212.705 kb on - strand, within DVU2117 at 2212.788 kb on + strand, within DVU2117 at 2213.009 kb on - strand at 2213.045 kb on + strand at 2213.055 kb on + strand at 2213.078 kb on + strand at 2213.102 kb on - strand at 2213.139 kb on - strand at 2213.152 kb on - strand at 2213.162 kb on - strand, within DVU2118 at 2213.187 kb on + strand, within DVU2118 at 2213.253 kb on + strand, within DVU2118 at 2213.253 kb on + strand, within DVU2118 at 2213.253 kb on + strand, within DVU2118 at 2213.261 kb on - strand, within DVU2118 at 2213.261 kb on - strand, within DVU2118 at 2213.261 kb on - strand, within DVU2118 at 2213.336 kb on - strand, within DVU2118 at 2213.358 kb on + strand, within DVU2118 at 2213.399 kb on + strand, within DVU2118 at 2213.460 kb on + strand, within DVU2118 at 2213.469 kb on - strand, within DVU2118 at 2213.469 kb on - strand, within DVU2118 at 2213.541 kb on + strand, within DVU2118 at 2213.567 kb on - strand, within DVU2118 at 2213.643 kb on + strand, within DVU2118 at 2213.643 kb on + strand, within DVU2118 at 2213.651 kb on + strand, within DVU2118 at 2213.651 kb on + strand, within DVU2118 at 2213.651 kb on - strand, within DVU2118 at 2213.651 kb on - strand, within DVU2118 at 2213.663 kb on + strand, within DVU2118 at 2213.663 kb on + strand, within DVU2118 at 2213.671 kb on - strand, within DVU2118 at 2213.671 kb on - strand, within DVU2118 at 2213.671 kb on - strand, within DVU2118 at 2213.671 kb on - strand, within DVU2118 at 2213.724 kb on + strand, within DVU2118 at 2213.803 kb on + strand, within DVU2118 at 2213.805 kb on + strand, within DVU2118 at 2213.930 kb on + strand at 2213.950 kb on + strand at 2213.950 kb on - strand at 2213.950 kb on - strand at 2213.955 kb on + strand at 2213.955 kb on + strand at 2213.960 kb on + strand at 2213.960 kb on + strand at 2213.970 kb on + strand at 2214.122 kb on + strand, within cpaC at 2214.165 kb on - strand, within cpaC at 2214.204 kb on + strand, within cpaC at 2214.212 kb on - strand, within cpaC at 2214.230 kb on + strand, within cpaC at 2214.230 kb on + strand, within cpaC at 2214.353 kb on - strand, within cpaC at 2214.388 kb on + strand, within cpaC at 2214.597 kb on - strand, within cpaC at 2214.831 kb on + strand, within cpaC at 2214.853 kb on + strand, within cpaC at 2214.861 kb on - strand, within cpaC at 2214.865 kb on + strand, within cpaC at 2214.887 kb on - strand, within cpaC
Per-strain Table
Position Strand Gene LocusTag Fraction MoLS4 with Cytosine 20mM (N) remove 2,212,121 + DVU2116 0.11 -0.6 2,212,197 + DVU2116 0.55 -0.1 2,212,213 + DVU2116 0.64 -0.7 2,212,216 - DVU2116 0.66 -0.6 2,212,221 - DVU2116 0.69 -0.9 2,212,269 + +0.8 2,212,283 - -1.8 2,212,301 + -0.3 2,212,310 + -0.1 2,212,339 + -1.9 2,212,347 + -1.0 2,212,347 + +1.0 2,212,362 - +1.7 2,212,412 + -1.0 2,212,506 + -1.0 2,212,571 + DVU2117 0.13 +0.2 2,212,617 + DVU2117 0.22 +1.2 2,212,624 + DVU2117 0.23 +0.3 2,212,672 - DVU2117 0.31 -0.2 2,212,698 - DVU2117 0.36 -1.9 2,212,705 - DVU2117 0.37 -0.0 2,212,788 + DVU2117 0.52 +0.4 2,213,009 - +0.7 2,213,045 + -0.9 2,213,055 + -0.5 2,213,078 + +0.1 2,213,102 - +0.0 2,213,139 - +0.3 2,213,152 - +0.1 2,213,162 - DVU2118 0.11 +0.2 2,213,187 + DVU2118 0.14 -1.8 2,213,253 + DVU2118 0.22 +1.1 2,213,253 + DVU2118 0.22 +0.1 2,213,253 + DVU2118 0.22 -0.3 2,213,261 - DVU2118 0.23 +1.7 2,213,261 - DVU2118 0.23 +1.7 2,213,261 - DVU2118 0.23 -0.0 2,213,336 - DVU2118 0.32 -1.7 2,213,358 + DVU2118 0.35 -2.5 2,213,399 + DVU2118 0.40 -0.5 2,213,460 + DVU2118 0.47 -0.2 2,213,469 - DVU2118 0.49 +0.5 2,213,469 - DVU2118 0.49 -0.2 2,213,541 + DVU2118 0.57 +0.5 2,213,567 - DVU2118 0.61 +0.7 2,213,643 + DVU2118 0.70 +0.7 2,213,643 + DVU2118 0.70 +0.2 2,213,651 + DVU2118 0.71 -1.1 2,213,651 + DVU2118 0.71 +2.3 2,213,651 - DVU2118 0.71 +0.1 2,213,651 - DVU2118 0.71 +0.1 2,213,663 + DVU2118 0.72 +0.3 2,213,663 + DVU2118 0.72 -3.6 2,213,671 - DVU2118 0.73 -1.4 2,213,671 - DVU2118 0.73 +1.1 2,213,671 - DVU2118 0.73 +0.1 2,213,671 - DVU2118 0.73 -1.1 2,213,724 + DVU2118 0.80 +0.9 2,213,803 + DVU2118 0.89 -0.4 2,213,805 + DVU2118 0.90 +0.2 2,213,930 + -0.6 2,213,950 + -1.0 2,213,950 - -0.6 2,213,950 - -0.3 2,213,955 + -1.5 2,213,955 + -0.4 2,213,960 + -0.0 2,213,960 + +0.3 2,213,970 + -0.5 2,214,122 + cpaC DVU2119 0.16 -1.2 2,214,165 - cpaC DVU2119 0.19 -0.4 2,214,204 + cpaC DVU2119 0.22 -1.1 2,214,212 - cpaC DVU2119 0.22 -0.2 2,214,230 + cpaC DVU2119 0.24 +0.6 2,214,230 + cpaC DVU2119 0.24 -0.1 2,214,353 - cpaC DVU2119 0.32 -0.1 2,214,388 + cpaC DVU2119 0.35 +1.1 2,214,597 - cpaC DVU2119 0.49 +0.2 2,214,831 + cpaC DVU2119 0.65 -0.7 2,214,853 + cpaC DVU2119 0.66 +0.5 2,214,861 - cpaC DVU2119 0.67 -1.1 2,214,865 + cpaC DVU2119 0.67 +0.2 2,214,887 - cpaC DVU2119 0.69 -2.5
Or see this region's nucleotide sequence