Experiment: MoLS4 with Cytosine 20mM (N)
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt DVU0826 and DVU0827 are separated by 147 nucleotides DVU0827 and smpB are separated by 28 nucleotides smpB and ptsI are separated by 11 nucleotides
DVU0826: DVU0826 - glycolate oxidase, iron-sulfur subunit, putative (TIGR), at 915,709 to 917,031
DVU0826
DVU0827: DVU0827 - glycolate oxidase, subunit GlcD, putative (TIGR), at 917,179 to 918,594
DVU0827
DVU0828: smpB - SsrA-binding protein (TIGR), at 918,623 to 919,087
smpB
DVU0829: ptsI - phosphoenolpyruvate-protein phosphotransferase (TIGR), at 919,099 to 920,871
ptsI
Position (kb) 917
918
919 Strain fitness (log2 ratio) -4
-3
-2
-1
0
1 at 916.264 kb on + strand, within DVU0826 at 916.276 kb on - strand, within DVU0826 at 916.329 kb on + strand, within DVU0826 at 916.362 kb on + strand, within DVU0826 at 916.362 kb on + strand, within DVU0826 at 916.416 kb on - strand, within DVU0826 at 916.416 kb on - strand, within DVU0826 at 916.503 kb on + strand, within DVU0826 at 916.525 kb on - strand, within DVU0826 at 916.543 kb on + strand, within DVU0826 at 916.697 kb on - strand, within DVU0826 at 916.700 kb on + strand, within DVU0826 at 916.719 kb on + strand, within DVU0826 at 916.719 kb on + strand, within DVU0826 at 916.719 kb on + strand, within DVU0826 at 916.901 kb on - strand at 916.959 kb on + strand at 917.019 kb on + strand at 917.034 kb on + strand at 917.048 kb on - strand at 917.066 kb on - strand at 917.222 kb on - strand at 917.225 kb on + strand at 917.225 kb on + strand at 917.233 kb on - strand at 917.233 kb on - strand at 917.233 kb on - strand at 917.233 kb on - strand at 917.233 kb on - strand at 917.233 kb on - strand at 917.233 kb on - strand at 917.238 kb on - strand at 917.285 kb on + strand at 917.290 kb on + strand at 917.293 kb on - strand at 917.305 kb on + strand at 917.351 kb on + strand, within DVU0827 at 917.358 kb on - strand, within DVU0827 at 917.465 kb on - strand, within DVU0827 at 917.601 kb on - strand, within DVU0827 at 917.675 kb on - strand, within DVU0827 at 917.717 kb on - strand, within DVU0827 at 917.749 kb on - strand, within DVU0827 at 917.779 kb on + strand, within DVU0827 at 917.779 kb on + strand, within DVU0827 at 917.787 kb on - strand, within DVU0827 at 917.787 kb on - strand, within DVU0827 at 917.859 kb on - strand, within DVU0827 at 917.859 kb on - strand, within DVU0827 at 917.900 kb on + strand, within DVU0827 at 917.906 kb on + strand, within DVU0827 at 917.952 kb on - strand, within DVU0827 at 918.059 kb on + strand, within DVU0827 at 918.065 kb on - strand, within DVU0827 at 918.199 kb on + strand, within DVU0827 at 918.561 kb on - strand at 918.592 kb on - strand at 918.602 kb on + strand at 918.602 kb on + strand at 918.602 kb on + strand at 918.610 kb on - strand at 918.610 kb on - strand at 918.620 kb on - strand at 918.621 kb on + strand at 919.172 kb on + strand at 919.194 kb on - strand at 919.202 kb on + strand at 919.212 kb on + strand at 919.212 kb on + strand at 919.244 kb on - strand at 919.407 kb on + strand, within ptsI at 919.440 kb on - strand, within ptsI at 919.555 kb on + strand, within ptsI at 919.583 kb on + strand, within ptsI
Per-strain Table
Position Strand Gene LocusTag Fraction MoLS4 with Cytosine 20mM (N) remove 916,264 + DVU0826 0.42 +0.2 916,276 - DVU0826 0.43 -1.1 916,329 + DVU0826 0.47 +0.4 916,362 + DVU0826 0.49 -0.0 916,362 + DVU0826 0.49 -0.1 916,416 - DVU0826 0.53 -0.4 916,416 - DVU0826 0.53 +0.4 916,503 + DVU0826 0.60 -2.0 916,525 - DVU0826 0.62 -0.3 916,543 + DVU0826 0.63 -0.5 916,697 - DVU0826 0.75 -1.1 916,700 + DVU0826 0.75 -0.5 916,719 + DVU0826 0.76 +1.0 916,719 + DVU0826 0.76 -1.0 916,719 + DVU0826 0.76 +0.1 916,901 - -1.6 916,959 + -0.3 917,019 + -0.5 917,034 + +0.3 917,048 - +0.7 917,066 - +0.1 917,222 - +0.1 917,225 + +1.0 917,225 + +0.2 917,233 - -0.4 917,233 - +0.8 917,233 - +0.1 917,233 - -0.1 917,233 - +1.2 917,233 - +0.2 917,233 - +0.1 917,238 - -1.2 917,285 + -0.0 917,290 + +0.3 917,293 - -0.1 917,305 + +1.1 917,351 + DVU0827 0.12 -0.5 917,358 - DVU0827 0.13 +0.0 917,465 - DVU0827 0.20 -0.2 917,601 - DVU0827 0.30 -2.1 917,675 - DVU0827 0.35 -0.9 917,717 - DVU0827 0.38 -0.1 917,749 - DVU0827 0.40 +0.1 917,779 + DVU0827 0.42 -0.1 917,779 + DVU0827 0.42 -0.3 917,787 - DVU0827 0.43 -0.2 917,787 - DVU0827 0.43 -0.6 917,859 - DVU0827 0.48 +0.1 917,859 - DVU0827 0.48 -0.7 917,900 + DVU0827 0.51 -0.8 917,906 + DVU0827 0.51 -0.2 917,952 - DVU0827 0.55 -0.2 918,059 + DVU0827 0.62 -0.2 918,065 - DVU0827 0.63 -0.5 918,199 + DVU0827 0.72 -0.8 918,561 - -0.0 918,592 - +0.0 918,602 + -1.0 918,602 + +0.3 918,602 + +0.1 918,610 - -2.2 918,610 - -0.6 918,620 - -0.3 918,621 + +0.5 919,172 + -0.0 919,194 - -0.5 919,202 + -0.3 919,212 + -0.3 919,212 + -2.8 919,244 - -2.1 919,407 + ptsI DVU0829 0.17 -4.0 919,440 - ptsI DVU0829 0.19 -2.3 919,555 + ptsI DVU0829 0.26 -2.2 919,583 + ptsI DVU0829 0.27 -3.2
Or see this region's nucleotide sequence