Experiment: MoLS4 with N2 (N)
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt DVU1919 and hynB-1 are separated by 317 nucleotides hynB-1 and hynA-1 are separated by 51 nucleotides
DVU1919: DVU1919 - hydrogenase expression/formation protein, putative (TIGR), at 1,993,855 to 1,994,325
DVU1919
DVU1921: hynB-1 - periplasmic [NiFe] hydrogenase, small subunit, isozyme 1 (TIGR), at 1,994,643 to 1,995,596
hynB-1
DVU1922: hynA-1 - periplasmic [NiFe] hydrogenase, large subunit, isozyme 1 (TIGR), at 1,995,648 to 1,997,348
hynA-1
Position (kb)
1994
1995
1996 Strain fitness (log2 ratio)
-2
-1
0
1
2 at 1993.711 kb on + strand at 1993.729 kb on + strand at 1993.768 kb on + strand at 1993.814 kb on + strand at 1993.959 kb on + strand, within DVU1919 at 1994.069 kb on - strand, within DVU1919 at 1994.184 kb on - strand, within DVU1919 at 1994.823 kb on + strand, within hynB-1 at 1994.828 kb on + strand, within hynB-1 at 1994.851 kb on + strand, within hynB-1 at 1994.859 kb on - strand, within hynB-1 at 1995.072 kb on + strand, within hynB-1 at 1995.104 kb on + strand, within hynB-1 at 1995.104 kb on + strand, within hynB-1 at 1995.113 kb on - strand, within hynB-1 at 1995.159 kb on + strand, within hynB-1 at 1995.169 kb on + strand, within hynB-1 at 1995.186 kb on - strand, within hynB-1 at 1995.460 kb on - strand, within hynB-1 at 1995.499 kb on - strand, within hynB-1 at 1995.509 kb on + strand at 1995.533 kb on + strand at 1995.541 kb on - strand at 1995.670 kb on - strand at 1995.711 kb on - strand at 1995.737 kb on - strand at 1995.785 kb on + strand at 1995.793 kb on - strand at 1995.793 kb on - strand at 1995.815 kb on + strand at 1995.832 kb on - strand, within hynA-1 at 1995.866 kb on + strand, within hynA-1 at 1995.868 kb on + strand, within hynA-1 at 1995.901 kb on - strand, within hynA-1 at 1996.035 kb on - strand, within hynA-1 at 1996.085 kb on + strand, within hynA-1 at 1996.085 kb on + strand, within hynA-1 at 1996.092 kb on + strand, within hynA-1 at 1996.134 kb on - strand, within hynA-1 at 1996.150 kb on - strand, within hynA-1 at 1996.150 kb on - strand, within hynA-1 at 1996.150 kb on - strand, within hynA-1 at 1996.157 kb on + strand, within hynA-1 at 1996.165 kb on - strand, within hynA-1 at 1996.170 kb on - strand, within hynA-1 at 1996.287 kb on - strand, within hynA-1 at 1996.301 kb on + strand, within hynA-1 at 1996.309 kb on - strand, within hynA-1 at 1996.309 kb on - strand, within hynA-1 at 1996.345 kb on + strand, within hynA-1 at 1996.372 kb on - strand, within hynA-1 at 1996.404 kb on - strand, within hynA-1 at 1996.424 kb on + strand, within hynA-1 at 1996.436 kb on + strand, within hynA-1 at 1996.458 kb on + strand, within hynA-1 at 1996.499 kb on + strand, within hynA-1 at 1996.499 kb on + strand, within hynA-1 at 1996.504 kb on + strand, within hynA-1 at 1996.507 kb on - strand, within hynA-1 at 1996.509 kb on + strand, within hynA-1 at 1996.517 kb on - strand, within hynA-1 at 1996.529 kb on + strand, within hynA-1
Per-strain Table
Position Strand Gene LocusTag Fraction MoLS4 with N2 (N) remove 1,993,711 + +1.2 1,993,729 + -0.1 1,993,768 + +1.2 1,993,814 + -0.1 1,993,959 + DVU1919 0.22 -0.5 1,994,069 - DVU1919 0.45 -0.9 1,994,184 - DVU1919 0.70 -0.4 1,994,823 + hynB-1 DVU1921 0.19 -1.3 1,994,828 + hynB-1 DVU1921 0.19 -0.1 1,994,851 + hynB-1 DVU1921 0.22 -0.5 1,994,859 - hynB-1 DVU1921 0.23 +0.4 1,995,072 + hynB-1 DVU1921 0.45 -0.6 1,995,104 + hynB-1 DVU1921 0.48 -1.7 1,995,104 + hynB-1 DVU1921 0.48 +0.2 1,995,113 - hynB-1 DVU1921 0.49 -0.3 1,995,159 + hynB-1 DVU1921 0.54 -0.6 1,995,169 + hynB-1 DVU1921 0.55 -0.4 1,995,186 - hynB-1 DVU1921 0.57 +0.6 1,995,460 - hynB-1 DVU1921 0.86 -1.8 1,995,499 - hynB-1 DVU1921 0.90 -0.2 1,995,509 + +0.1 1,995,533 + +2.6 1,995,541 - +0.1 1,995,670 - +0.2 1,995,711 - -0.5 1,995,737 - +0.5 1,995,785 + -0.2 1,995,793 - +0.1 1,995,793 - -0.5 1,995,815 + -0.6 1,995,832 - hynA-1 DVU1922 0.11 -0.3 1,995,866 + hynA-1 DVU1922 0.13 -0.3 1,995,868 + hynA-1 DVU1922 0.13 +0.1 1,995,901 - hynA-1 DVU1922 0.15 -0.7 1,996,035 - hynA-1 DVU1922 0.23 -0.1 1,996,085 + hynA-1 DVU1922 0.26 -0.6 1,996,085 + hynA-1 DVU1922 0.26 +1.1 1,996,092 + hynA-1 DVU1922 0.26 +0.7 1,996,134 - hynA-1 DVU1922 0.29 -0.0 1,996,150 - hynA-1 DVU1922 0.30 -1.4 1,996,150 - hynA-1 DVU1922 0.30 +0.1 1,996,150 - hynA-1 DVU1922 0.30 -1.8 1,996,157 + hynA-1 DVU1922 0.30 -0.7 1,996,165 - hynA-1 DVU1922 0.30 -0.6 1,996,170 - hynA-1 DVU1922 0.31 +0.9 1,996,287 - hynA-1 DVU1922 0.38 +0.6 1,996,301 + hynA-1 DVU1922 0.38 +0.6 1,996,309 - hynA-1 DVU1922 0.39 +0.9 1,996,309 - hynA-1 DVU1922 0.39 +0.7 1,996,345 + hynA-1 DVU1922 0.41 -0.0 1,996,372 - hynA-1 DVU1922 0.43 +0.6 1,996,404 - hynA-1 DVU1922 0.44 +0.2 1,996,424 + hynA-1 DVU1922 0.46 +0.7 1,996,436 + hynA-1 DVU1922 0.46 -0.0 1,996,458 + hynA-1 DVU1922 0.48 -0.6 1,996,499 + hynA-1 DVU1922 0.50 +1.4 1,996,499 + hynA-1 DVU1922 0.50 +0.1 1,996,504 + hynA-1 DVU1922 0.50 +0.2 1,996,507 - hynA-1 DVU1922 0.50 -2.1 1,996,509 + hynA-1 DVU1922 0.51 +0.2 1,996,517 - hynA-1 DVU1922 0.51 -0.2 1,996,529 + hynA-1 DVU1922 0.52 -0.9
Or see this region's nucleotide sequence