Experiment: 10% P. fluorescens FW300-N2C3 spent media from growth in 20 mM putrescine
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt Pf6N2E2_924 and Pf6N2E2_925 are separated by 99 nucleotides
Pf6N2E2_924 - Transcriptional regulator XdhR, TetR family, at 1,088,150 to 1,088,737
_924
Pf6N2E2_925 - Sorbitol-6-phosphate 2-dehydrogenase (EC 1.1.1.140), at 1,088,837 to 1,089,595
_925
Position (kb)
1088
1089 Strain fitness (log2 ratio)
-2
-1
0
1
2 at 1087.193 kb on + strand at 1087.194 kb on - strand at 1087.254 kb on - strand at 1087.290 kb on + strand at 1087.323 kb on - strand at 1087.323 kb on - strand at 1087.345 kb on + strand at 1087.354 kb on + strand at 1087.355 kb on - strand at 1087.487 kb on + strand at 1087.487 kb on + strand at 1087.487 kb on + strand at 1087.550 kb on - strand at 1087.614 kb on + strand at 1087.614 kb on + strand at 1087.615 kb on - strand at 1087.615 kb on - strand at 1087.617 kb on + strand at 1087.617 kb on + strand at 1087.618 kb on - strand at 1087.618 kb on - strand at 1087.618 kb on - strand at 1087.618 kb on - strand at 1087.624 kb on + strand at 1087.624 kb on + strand at 1087.624 kb on + strand at 1087.625 kb on - strand at 1087.625 kb on - strand at 1087.917 kb on + strand at 1087.918 kb on - strand at 1087.924 kb on - strand at 1087.947 kb on + strand at 1088.058 kb on + strand at 1088.058 kb on + strand at 1088.059 kb on - strand at 1088.077 kb on + strand at 1088.078 kb on - strand at 1088.089 kb on - strand at 1088.089 kb on - strand at 1088.093 kb on - strand at 1088.093 kb on - strand at 1088.124 kb on + strand at 1088.145 kb on + strand at 1088.146 kb on - strand at 1088.151 kb on + strand at 1088.151 kb on + strand at 1088.448 kb on + strand, within Pf6N2E2_924 at 1088.449 kb on - strand, within Pf6N2E2_924 at 1088.489 kb on - strand, within Pf6N2E2_924 at 1088.833 kb on + strand at 1088.833 kb on + strand at 1088.837 kb on - strand at 1088.837 kb on - strand at 1088.846 kb on - strand at 1088.846 kb on - strand at 1088.876 kb on - strand at 1088.885 kb on - strand at 1088.905 kb on + strand at 1088.906 kb on - strand at 1088.906 kb on - strand at 1088.906 kb on - strand at 1088.906 kb on - strand at 1089.085 kb on - strand, within Pf6N2E2_925 at 1089.168 kb on + strand, within Pf6N2E2_925 at 1089.168 kb on + strand, within Pf6N2E2_925 at 1089.169 kb on - strand at 1089.169 kb on - strand, within Pf6N2E2_925 at 1089.184 kb on + strand, within Pf6N2E2_925 at 1089.185 kb on - strand, within Pf6N2E2_925 at 1089.192 kb on - strand, within Pf6N2E2_925 at 1089.192 kb on - strand, within Pf6N2E2_925 at 1089.192 kb on - strand, within Pf6N2E2_925 at 1089.192 kb on - strand, within Pf6N2E2_925 at 1089.192 kb on - strand, within Pf6N2E2_925 at 1089.192 kb on - strand, within Pf6N2E2_925 at 1089.199 kb on + strand, within Pf6N2E2_925 at 1089.288 kb on - strand, within Pf6N2E2_925 at 1089.288 kb on - strand, within Pf6N2E2_925 at 1089.288 kb on - strand, within Pf6N2E2_925 at 1089.288 kb on - strand, within Pf6N2E2_925 at 1089.342 kb on + strand, within Pf6N2E2_925 at 1089.343 kb on - strand, within Pf6N2E2_925 at 1089.387 kb on + strand, within Pf6N2E2_925 at 1089.388 kb on - strand, within Pf6N2E2_925 at 1089.391 kb on + strand, within Pf6N2E2_925 at 1089.391 kb on + strand, within Pf6N2E2_925 at 1089.391 kb on + strand, within Pf6N2E2_925 at 1089.392 kb on - strand, within Pf6N2E2_925 at 1089.392 kb on - strand, within Pf6N2E2_925 at 1089.392 kb on - strand, within Pf6N2E2_925 at 1089.392 kb on - strand, within Pf6N2E2_925 at 1089.392 kb on - strand, within Pf6N2E2_925 at 1089.395 kb on - strand, within Pf6N2E2_925 at 1089.395 kb on - strand, within Pf6N2E2_925 at 1089.395 kb on - strand, within Pf6N2E2_925 at 1089.395 kb on - strand, within Pf6N2E2_925 at 1089.395 kb on - strand, within Pf6N2E2_925 at 1089.447 kb on + strand, within Pf6N2E2_925 at 1089.575 kb on + strand at 1089.601 kb on - strand at 1089.607 kb on + strand at 1089.683 kb on + strand
Per-strain Table
Position Strand Gene LocusTag Fraction 10% P. fluorescens FW300-N2C3 spent media from growth in 20 mM putrescine remove 1,087,193 + +0.4 1,087,194 - -0.5 1,087,254 - +0.1 1,087,290 + -0.5 1,087,323 - -0.5 1,087,323 - +0.3 1,087,345 + +0.8 1,087,354 + -0.5 1,087,355 - -0.5 1,087,487 + +0.4 1,087,487 + -0.4 1,087,487 + -0.5 1,087,550 - -1.1 1,087,614 + -0.0 1,087,614 + +1.1 1,087,615 - -1.5 1,087,615 - -0.5 1,087,617 + -0.5 1,087,617 + +0.5 1,087,618 - -0.5 1,087,618 - +1.1 1,087,618 - -0.5 1,087,618 - -1.1 1,087,624 + -0.3 1,087,624 + +0.8 1,087,624 + -0.3 1,087,625 - +1.8 1,087,625 - -0.0 1,087,917 + -0.5 1,087,918 - +0.5 1,087,924 - +0.1 1,087,947 + +1.1 1,088,058 + -0.5 1,088,058 + +0.5 1,088,059 - +1.1 1,088,077 + -0.5 1,088,078 - +0.6 1,088,089 - -0.5 1,088,089 - -0.5 1,088,093 - +0.2 1,088,093 - +0.8 1,088,124 + +0.2 1,088,145 + -0.1 1,088,146 - +0.1 1,088,151 + -0.5 1,088,151 + -0.5 1,088,448 + Pf6N2E2_924 0.51 -0.5 1,088,449 - Pf6N2E2_924 0.51 -0.5 1,088,489 - Pf6N2E2_924 0.58 -1.3 1,088,833 + +0.5 1,088,833 + -2.5 1,088,837 - +0.3 1,088,837 - +0.3 1,088,846 - +0.2 1,088,846 - +0.6 1,088,876 - -0.1 1,088,885 - -0.2 1,088,905 + +0.3 1,088,906 - -0.5 1,088,906 - +0.7 1,088,906 - +1.1 1,088,906 - +1.1 1,089,085 - Pf6N2E2_925 0.33 +1.4 1,089,168 + Pf6N2E2_925 0.44 -0.5 1,089,168 + Pf6N2E2_925 0.44 -0.6 1,089,169 - -1.9 1,089,169 - Pf6N2E2_925 0.44 -0.5 1,089,184 + Pf6N2E2_925 0.46 +1.1 1,089,185 - Pf6N2E2_925 0.46 +0.5 1,089,192 - Pf6N2E2_925 0.47 +0.3 1,089,192 - Pf6N2E2_925 0.47 +1.2 1,089,192 - Pf6N2E2_925 0.47 -0.1 1,089,192 - Pf6N2E2_925 0.47 -1.3 1,089,192 - Pf6N2E2_925 0.47 -0.7 1,089,192 - Pf6N2E2_925 0.47 +1.1 1,089,199 + Pf6N2E2_925 0.48 +0.2 1,089,288 - Pf6N2E2_925 0.59 -0.5 1,089,288 - Pf6N2E2_925 0.59 +2.3 1,089,288 - Pf6N2E2_925 0.59 -0.3 1,089,288 - Pf6N2E2_925 0.59 +0.6 1,089,342 + Pf6N2E2_925 0.67 -1.3 1,089,343 - Pf6N2E2_925 0.67 -1.5 1,089,387 + Pf6N2E2_925 0.72 -0.1 1,089,388 - Pf6N2E2_925 0.73 +1.3 1,089,391 + Pf6N2E2_925 0.73 -0.1 1,089,391 + Pf6N2E2_925 0.73 +0.3 1,089,391 + Pf6N2E2_925 0.73 +0.5 1,089,392 - Pf6N2E2_925 0.73 +0.5 1,089,392 - Pf6N2E2_925 0.73 +0.6 1,089,392 - Pf6N2E2_925 0.73 +1.3 1,089,392 - Pf6N2E2_925 0.73 +0.4 1,089,392 - Pf6N2E2_925 0.73 +0.1 1,089,395 - Pf6N2E2_925 0.74 -0.5 1,089,395 - Pf6N2E2_925 0.74 -0.5 1,089,395 - Pf6N2E2_925 0.74 +1.1 1,089,395 - Pf6N2E2_925 0.74 +0.5 1,089,395 - Pf6N2E2_925 0.74 +0.7 1,089,447 + Pf6N2E2_925 0.80 +1.8 1,089,575 + -0.9 1,089,601 - +0.1 1,089,607 + +0.8 1,089,683 + -0.5
Or see this region's nucleotide sequence