Experiment: 10% P. fluorescens FW300-N2C3 spent media from growth in 20 mM putrescine
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt Pf6N2E2_894 and Pf6N2E2_895 are separated by 367 nucleotides Pf6N2E2_895 and Pf6N2E2_896 are separated by 141 nucleotides
Pf6N2E2_894 - Beta-lactamase-like, at 1,047,281 to 1,048,165
_894
Pf6N2E2_895 - Transcriptional regulator, HxlR family, at 1,048,533 to 1,049,018
_895
Pf6N2E2_896 - Regulatory protein, LysR:LysR, substrate-binding, at 1,049,160 to 1,050,077
_896
Position (kb)
1048
1049
1050 Strain fitness (log2 ratio)
-3
-2
-1
0
1
2
3 at 1047.555 kb on + strand, within Pf6N2E2_894 at 1047.556 kb on - strand, within Pf6N2E2_894 at 1047.556 kb on - strand, within Pf6N2E2_894 at 1047.591 kb on + strand, within Pf6N2E2_894 at 1047.592 kb on - strand, within Pf6N2E2_894 at 1047.592 kb on - strand, within Pf6N2E2_894 at 1047.630 kb on + strand, within Pf6N2E2_894 at 1047.631 kb on - strand, within Pf6N2E2_894 at 1047.657 kb on + strand, within Pf6N2E2_894 at 1047.657 kb on + strand, within Pf6N2E2_894 at 1047.657 kb on + strand, within Pf6N2E2_894 at 1047.657 kb on + strand, within Pf6N2E2_894 at 1047.658 kb on - strand, within Pf6N2E2_894 at 1047.804 kb on + strand, within Pf6N2E2_894 at 1047.805 kb on - strand, within Pf6N2E2_894 at 1047.871 kb on - strand, within Pf6N2E2_894 at 1047.910 kb on + strand, within Pf6N2E2_894 at 1048.098 kb on + strand at 1048.098 kb on + strand at 1048.098 kb on + strand at 1048.099 kb on - strand at 1048.116 kb on + strand at 1048.116 kb on + strand at 1048.137 kb on + strand at 1048.190 kb on + strand at 1048.191 kb on - strand at 1048.191 kb on - strand at 1048.191 kb on - strand at 1048.293 kb on + strand at 1048.293 kb on + strand at 1048.293 kb on + strand at 1048.293 kb on + strand at 1048.293 kb on + strand at 1048.293 kb on + strand at 1048.293 kb on + strand at 1048.294 kb on - strand at 1048.294 kb on - strand at 1048.294 kb on - strand at 1048.295 kb on + strand at 1048.295 kb on + strand at 1048.295 kb on + strand at 1048.295 kb on + strand at 1048.295 kb on + strand at 1048.295 kb on + strand at 1048.295 kb on + strand at 1048.295 kb on + strand at 1048.295 kb on + strand at 1048.296 kb on - strand at 1048.296 kb on - strand at 1048.296 kb on - strand at 1048.296 kb on - strand at 1048.296 kb on - strand at 1048.296 kb on - strand at 1048.297 kb on + strand at 1048.297 kb on + strand at 1048.297 kb on + strand at 1048.297 kb on + strand at 1048.297 kb on + strand at 1048.297 kb on + strand at 1048.297 kb on + strand at 1048.297 kb on + strand at 1048.297 kb on + strand at 1048.297 kb on + strand at 1048.297 kb on + strand at 1048.297 kb on + strand at 1048.297 kb on + strand at 1048.297 kb on + strand at 1048.297 kb on + strand at 1048.297 kb on + strand at 1048.297 kb on + strand at 1048.297 kb on + strand at 1048.297 kb on + strand at 1048.297 kb on + strand at 1048.297 kb on + strand at 1048.297 kb on + strand at 1048.297 kb on + strand at 1048.298 kb on - strand at 1048.298 kb on - strand at 1048.298 kb on - strand at 1048.298 kb on - strand at 1048.298 kb on - strand at 1048.298 kb on - strand at 1048.298 kb on - strand at 1048.298 kb on - strand at 1048.298 kb on - strand at 1048.298 kb on - strand at 1048.298 kb on - strand at 1048.301 kb on + strand at 1048.301 kb on + strand at 1048.301 kb on + strand at 1048.301 kb on + strand at 1048.301 kb on + strand at 1048.301 kb on + strand at 1048.302 kb on - strand at 1048.302 kb on - strand at 1048.302 kb on - strand at 1048.302 kb on - strand at 1048.302 kb on - strand at 1048.302 kb on - strand at 1048.305 kb on + strand at 1048.305 kb on + strand at 1048.305 kb on + strand at 1048.306 kb on - strand at 1048.306 kb on - strand at 1048.306 kb on - strand at 1048.306 kb on - strand at 1048.306 kb on - strand at 1048.313 kb on + strand at 1048.313 kb on + strand at 1048.314 kb on - strand at 1048.375 kb on + strand at 1048.376 kb on - strand at 1048.482 kb on + strand at 1048.482 kb on + strand at 1048.482 kb on + strand at 1048.485 kb on + strand at 1048.528 kb on + strand at 1048.529 kb on - strand at 1048.529 kb on - strand at 1048.529 kb on - strand at 1048.529 kb on - strand at 1048.645 kb on - strand, within Pf6N2E2_895 at 1048.768 kb on + strand, within Pf6N2E2_895 at 1048.768 kb on + strand, within Pf6N2E2_895 at 1048.795 kb on + strand, within Pf6N2E2_895 at 1048.795 kb on + strand, within Pf6N2E2_895 at 1048.795 kb on + strand, within Pf6N2E2_895 at 1048.818 kb on + strand, within Pf6N2E2_895 at 1048.818 kb on + strand, within Pf6N2E2_895 at 1048.819 kb on - strand, within Pf6N2E2_895 at 1048.930 kb on + strand, within Pf6N2E2_895 at 1048.930 kb on + strand, within Pf6N2E2_895 at 1048.930 kb on + strand, within Pf6N2E2_895 at 1048.930 kb on + strand, within Pf6N2E2_895 at 1048.935 kb on + strand, within Pf6N2E2_895 at 1049.013 kb on - strand at 1049.016 kb on + strand at 1049.016 kb on + strand at 1049.016 kb on + strand at 1049.016 kb on + strand at 1049.016 kb on + strand at 1049.016 kb on + strand at 1049.016 kb on + strand at 1049.017 kb on - strand at 1049.017 kb on - strand at 1049.017 kb on - strand at 1049.017 kb on - strand at 1049.017 kb on - strand at 1049.017 kb on - strand at 1049.063 kb on - strand at 1049.076 kb on + strand at 1049.077 kb on - strand at 1049.161 kb on + strand at 1049.161 kb on + strand at 1049.162 kb on - strand at 1049.227 kb on - strand at 1049.358 kb on + strand, within Pf6N2E2_896 at 1049.467 kb on + strand, within Pf6N2E2_896 at 1049.689 kb on + strand, within Pf6N2E2_896 at 1049.690 kb on - strand, within Pf6N2E2_896 at 1049.744 kb on + strand, within Pf6N2E2_896 at 1049.744 kb on + strand, within Pf6N2E2_896 at 1049.786 kb on - strand, within Pf6N2E2_896 at 1049.848 kb on - strand, within Pf6N2E2_896
Per-strain Table
Position Strand Gene LocusTag Fraction 10% P. fluorescens FW300-N2C3 spent media from growth in 20 mM putrescine remove 1,047,555 + Pf6N2E2_894 0.31 -1.5 1,047,556 - Pf6N2E2_894 0.31 -0.5 1,047,556 - Pf6N2E2_894 0.31 -0.5 1,047,591 + Pf6N2E2_894 0.35 -1.1 1,047,592 - Pf6N2E2_894 0.35 +0.5 1,047,592 - Pf6N2E2_894 0.35 +0.6 1,047,630 + Pf6N2E2_894 0.39 +1.5 1,047,631 - Pf6N2E2_894 0.40 +0.2 1,047,657 + Pf6N2E2_894 0.42 +0.6 1,047,657 + Pf6N2E2_894 0.42 -1.5 1,047,657 + Pf6N2E2_894 0.42 -1.5 1,047,657 + Pf6N2E2_894 0.42 -0.5 1,047,658 - Pf6N2E2_894 0.43 +0.1 1,047,804 + Pf6N2E2_894 0.59 +0.3 1,047,805 - Pf6N2E2_894 0.59 -1.0 1,047,871 - Pf6N2E2_894 0.67 -1.0 1,047,910 + Pf6N2E2_894 0.71 +0.0 1,048,098 + +0.3 1,048,098 + +0.7 1,048,098 + -0.3 1,048,099 - +0.1 1,048,116 + -0.4 1,048,116 + +1.1 1,048,137 + -0.3 1,048,190 + +0.2 1,048,191 - +0.2 1,048,191 - -0.5 1,048,191 - -2.1 1,048,293 + +1.8 1,048,293 + +0.1 1,048,293 + -0.5 1,048,293 + +0.3 1,048,293 + -0.5 1,048,293 + -0.1 1,048,293 + -0.5 1,048,294 - -0.5 1,048,294 - -0.1 1,048,294 - +0.7 1,048,295 + +3.3 1,048,295 + +1.2 1,048,295 + +0.1 1,048,295 + -0.0 1,048,295 + +0.7 1,048,295 + +1.8 1,048,295 + +1.0 1,048,295 + +1.8 1,048,295 + +0.5 1,048,296 - -0.9 1,048,296 - +0.9 1,048,296 - +0.7 1,048,296 - +0.5 1,048,296 - -0.1 1,048,296 - +0.4 1,048,297 + -0.1 1,048,297 + +1.8 1,048,297 + +0.5 1,048,297 + -0.3 1,048,297 + +0.3 1,048,297 + +0.1 1,048,297 + -0.4 1,048,297 + +1.3 1,048,297 + +1.7 1,048,297 + +0.5 1,048,297 + -0.7 1,048,297 + +0.8 1,048,297 + +1.5 1,048,297 + +0.9 1,048,297 + -0.2 1,048,297 + +2.3 1,048,297 + +0.3 1,048,297 + +1.2 1,048,297 + +0.7 1,048,297 + +1.1 1,048,297 + -0.2 1,048,297 + +2.8 1,048,297 + -0.9 1,048,298 - -1.3 1,048,298 - +0.1 1,048,298 - -1.5 1,048,298 - +0.1 1,048,298 - -0.5 1,048,298 - -0.5 1,048,298 - -0.1 1,048,298 - +0.1 1,048,298 - +0.3 1,048,298 - -0.5 1,048,298 - +0.5 1,048,301 + +1.1 1,048,301 + -0.5 1,048,301 + -0.5 1,048,301 + +0.8 1,048,301 + +0.1 1,048,301 + +1.9 1,048,302 - -0.5 1,048,302 - +2.5 1,048,302 - -0.5 1,048,302 - +0.5 1,048,302 - -0.5 1,048,302 - +1.3 1,048,305 + +1.5 1,048,305 + +0.5 1,048,305 + +1.7 1,048,306 - +0.0 1,048,306 - -1.1 1,048,306 - -0.5 1,048,306 - +0.1 1,048,306 - +0.1 1,048,313 + -0.9 1,048,313 + +0.5 1,048,314 - -0.5 1,048,375 + -1.3 1,048,376 - +0.1 1,048,482 + +1.7 1,048,482 + +0.5 1,048,482 + +1.7 1,048,485 + +1.0 1,048,528 + -0.5 1,048,529 - -1.5 1,048,529 - -1.5 1,048,529 - +0.5 1,048,529 - -0.5 1,048,645 - Pf6N2E2_895 0.23 -0.1 1,048,768 + Pf6N2E2_895 0.48 -0.5 1,048,768 + Pf6N2E2_895 0.48 -0.5 1,048,795 + Pf6N2E2_895 0.54 -0.5 1,048,795 + Pf6N2E2_895 0.54 -1.5 1,048,795 + Pf6N2E2_895 0.54 -1.5 1,048,818 + Pf6N2E2_895 0.59 -0.5 1,048,818 + Pf6N2E2_895 0.59 -0.5 1,048,819 - Pf6N2E2_895 0.59 -2.8 1,048,930 + Pf6N2E2_895 0.82 -1.5 1,048,930 + Pf6N2E2_895 0.82 -0.5 1,048,930 + Pf6N2E2_895 0.82 -1.5 1,048,930 + Pf6N2E2_895 0.82 +0.1 1,048,935 + Pf6N2E2_895 0.83 -0.5 1,049,013 - -0.3 1,049,016 + +0.1 1,049,016 + -0.8 1,049,016 + -1.8 1,049,016 + -0.5 1,049,016 + -0.5 1,049,016 + -0.1 1,049,016 + +0.6 1,049,017 - -0.7 1,049,017 - +0.3 1,049,017 - +0.5 1,049,017 - +1.5 1,049,017 - +1.1 1,049,017 - +0.2 1,049,063 - +0.1 1,049,076 + -0.5 1,049,077 - -0.2 1,049,161 + -1.5 1,049,161 + +0.9 1,049,162 - -0.9 1,049,227 - -0.9 1,049,358 + Pf6N2E2_896 0.22 +0.8 1,049,467 + Pf6N2E2_896 0.33 -1.5 1,049,689 + Pf6N2E2_896 0.58 -0.5 1,049,690 - Pf6N2E2_896 0.58 +0.1 1,049,744 + Pf6N2E2_896 0.64 -0.2 1,049,744 + Pf6N2E2_896 0.64 -0.3 1,049,786 - Pf6N2E2_896 0.68 -0.4 1,049,848 - Pf6N2E2_896 0.75 +0.9
Or see this region's nucleotide sequence