Experiment: 10% P. fluorescens FW300-N2C3 spent media from growth in 20 mM putrescine
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt Pf6N2E2_77 and Pf6N2E2_78 overlap by 4 nucleotides Pf6N2E2_78 and Pf6N2E2_6100 are separated by 168 nucleotides Pf6N2E2_6100 and Pf6N2E2_6101 are separated by 61 nucleotides Pf6N2E2_6101 and Pf6N2E2_6102 are separated by 93 nucleotides Pf6N2E2_6102 and Pf6N2E2_79 are separated by 432 nucleotides
Pf6N2E2_77 - O-acetylhomoserine sulfhydrylase (EC 2.5.1.49) / O-succinylhomoserine sulfhydrylase (EC 2.5.1.48), at 119,551 to 120,762
_77
Pf6N2E2_78 - Oxidoreductase, short chain dehydrogenase/reductase family, at 120,759 to 121,532
_78
Pf6N2E2_6100 - tRNA-Val-TAC, at 121,701 to 121,773
_6100
Pf6N2E2_6101 - tRNA-Asp-GTC, at 121,835 to 121,908
_6101
Pf6N2E2_6102 - tRNA-Asp-GTC, at 122,002 to 122,075
_6102
Pf6N2E2_79 - Mobile element protein, at 122,508 to 123,776
_79
Position (kb)
121
122 Strain fitness (log2 ratio)
-2
-1
0
1
2 at 121.092 kb on + strand, within Pf6N2E2_78 at 121.159 kb on - strand, within Pf6N2E2_78 at 121.236 kb on + strand, within Pf6N2E2_78 at 121.236 kb on + strand, within Pf6N2E2_78 at 121.237 kb on - strand, within Pf6N2E2_78 at 121.237 kb on - strand, within Pf6N2E2_78 at 121.412 kb on - strand, within Pf6N2E2_78 at 121.421 kb on - strand, within Pf6N2E2_78 at 121.524 kb on + strand at 121.524 kb on + strand at 121.525 kb on - strand at 121.525 kb on - strand at 121.525 kb on - strand at 121.572 kb on + strand at 121.572 kb on + strand at 121.573 kb on - strand at 121.598 kb on + strand at 121.599 kb on - strand at 121.658 kb on - strand at 121.734 kb on + strand, within Pf6N2E2_6100 at 121.734 kb on + strand, within Pf6N2E2_6100 at 121.782 kb on + strand at 122.139 kb on + strand at 122.203 kb on + strand at 122.203 kb on + strand at 122.252 kb on - strand at 122.252 kb on - strand at 122.252 kb on - strand at 122.252 kb on - strand at 122.252 kb on - strand at 122.256 kb on - strand
Per-strain Table
Position Strand Gene LocusTag Fraction 10% P. fluorescens FW300-N2C3 spent media from growth in 20 mM putrescine remove 121,092 + Pf6N2E2_78 0.43 -1.4 121,159 - Pf6N2E2_78 0.52 -1.6 121,236 + Pf6N2E2_78 0.62 +0.4 121,236 + Pf6N2E2_78 0.62 -1.0 121,237 - Pf6N2E2_78 0.62 -0.2 121,237 - Pf6N2E2_78 0.62 -1.1 121,412 - Pf6N2E2_78 0.84 -0.2 121,421 - Pf6N2E2_78 0.86 -1.3 121,524 + +1.8 121,524 + -0.2 121,525 - -0.0 121,525 - -1.0 121,525 - -2.2 121,572 + +0.6 121,572 + +0.4 121,573 - -1.2 121,598 + -0.2 121,599 - +0.3 121,658 - -0.2 121,734 + Pf6N2E2_6100 0.45 -0.7 121,734 + Pf6N2E2_6100 0.45 -0.6 121,782 + -0.2 122,139 + -1.0 122,203 + -0.2 122,203 + -0.5 122,252 - +0.4 122,252 - +1.1 122,252 - +0.6 122,252 - -0.2 122,252 - +0.4 122,256 - +0.5
Or see this region's nucleotide sequence