Experiment: 10% P. fluorescens FW300-N2C3 spent media from growth in 20 mM putrescine
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt Pf6N2E2_6159 and Pf6N2E2_6160 are separated by 75 nucleotides Pf6N2E2_6160 and Pf6N2E2_5678 are separated by 43 nucleotides Pf6N2E2_5678 and Pf6N2E2_5679 are separated by 160 nucleotides Pf6N2E2_5679 and Pf6N2E2_5680 are separated by 21 nucleotides
Pf6N2E2_6159 - tRNA-Arg-ACG, at 6,491,920 to 6,491,993
_6159
Pf6N2E2_6160 - tRNA-Arg-ACG, at 6,492,069 to 6,492,142
_6160
Pf6N2E2_5678 - hypothetical protein, at 6,492,186 to 6,492,692
_5678
Pf6N2E2_5679 - hypothetical protein, at 6,492,853 to 6,492,993
_5679
Pf6N2E2_5680 - Mg/Co/Ni transporter MgtE / CBS domain, at 6,493,015 to 6,494,457
_5680
Position (kb) 6492
6493 Strain fitness (log2 ratio) -3
-2
-1
0
1 at 6491.999 kb on + strand at 6491.999 kb on + strand at 6492.024 kb on + strand at 6492.256 kb on + strand, within Pf6N2E2_5678 at 6492.256 kb on + strand, within Pf6N2E2_5678 at 6492.256 kb on + strand, within Pf6N2E2_5678 at 6492.256 kb on + strand, within Pf6N2E2_5678 at 6492.256 kb on + strand, within Pf6N2E2_5678 at 6492.256 kb on + strand, within Pf6N2E2_5678 at 6492.256 kb on + strand, within Pf6N2E2_5678 at 6492.256 kb on + strand, within Pf6N2E2_5678 at 6492.256 kb on + strand, within Pf6N2E2_5678 at 6492.257 kb on - strand, within Pf6N2E2_5678 at 6492.257 kb on - strand, within Pf6N2E2_5678 at 6492.257 kb on - strand, within Pf6N2E2_5678 at 6492.257 kb on - strand, within Pf6N2E2_5678 at 6492.257 kb on - strand, within Pf6N2E2_5678 at 6492.257 kb on - strand, within Pf6N2E2_5678 at 6492.257 kb on - strand, within Pf6N2E2_5678 at 6492.260 kb on + strand, within Pf6N2E2_5678 at 6492.260 kb on + strand, within Pf6N2E2_5678 at 6492.260 kb on + strand, within Pf6N2E2_5678 at 6492.261 kb on - strand, within Pf6N2E2_5678 at 6492.262 kb on + strand, within Pf6N2E2_5678 at 6492.318 kb on + strand, within Pf6N2E2_5678 at 6492.318 kb on + strand, within Pf6N2E2_5678 at 6492.318 kb on + strand, within Pf6N2E2_5678 at 6492.318 kb on + strand, within Pf6N2E2_5678 at 6492.552 kb on + strand, within Pf6N2E2_5678 at 6492.648 kb on + strand at 6492.649 kb on - strand at 6492.729 kb on - strand at 6492.729 kb on - strand at 6492.854 kb on + strand at 6492.871 kb on + strand, within Pf6N2E2_5679 at 6492.908 kb on + strand, within Pf6N2E2_5679 at 6492.908 kb on + strand, within Pf6N2E2_5679 at 6492.908 kb on + strand, within Pf6N2E2_5679 at 6492.910 kb on + strand, within Pf6N2E2_5679 at 6492.910 kb on + strand, within Pf6N2E2_5679 at 6492.922 kb on + strand, within Pf6N2E2_5679 at 6492.929 kb on + strand, within Pf6N2E2_5679 at 6492.929 kb on + strand, within Pf6N2E2_5679 at 6492.933 kb on + strand at 6492.943 kb on + strand, within Pf6N2E2_5679 at 6492.943 kb on + strand, within Pf6N2E2_5679 at 6492.964 kb on + strand, within Pf6N2E2_5679 at 6493.014 kb on + strand
Per-strain Table
Position Strand Gene LocusTag Fraction 10% P. fluorescens FW300-N2C3 spent media from growth in 20 mM putrescine remove 6,491,999 + +0.1 6,491,999 + +0.5 6,492,024 + -0.3 6,492,256 + Pf6N2E2_5678 0.14 +0.1 6,492,256 + Pf6N2E2_5678 0.14 +0.1 6,492,256 + Pf6N2E2_5678 0.14 +0.1 6,492,256 + Pf6N2E2_5678 0.14 +0.1 6,492,256 + Pf6N2E2_5678 0.14 +0.1 6,492,256 + Pf6N2E2_5678 0.14 +0.1 6,492,256 + Pf6N2E2_5678 0.14 +0.1 6,492,256 + Pf6N2E2_5678 0.14 -0.9 6,492,256 + Pf6N2E2_5678 0.14 +0.1 6,492,257 - Pf6N2E2_5678 0.14 +0.1 6,492,257 - Pf6N2E2_5678 0.14 +0.1 6,492,257 - Pf6N2E2_5678 0.14 +0.1 6,492,257 - Pf6N2E2_5678 0.14 +0.1 6,492,257 - Pf6N2E2_5678 0.14 +0.1 6,492,257 - Pf6N2E2_5678 0.14 +0.1 6,492,257 - Pf6N2E2_5678 0.14 +0.1 6,492,260 + Pf6N2E2_5678 0.15 +0.1 6,492,260 + Pf6N2E2_5678 0.15 +0.1 6,492,260 + Pf6N2E2_5678 0.15 +1.7 6,492,261 - Pf6N2E2_5678 0.15 +0.1 6,492,262 + Pf6N2E2_5678 0.15 -1.5 6,492,318 + Pf6N2E2_5678 0.26 +0.1 6,492,318 + Pf6N2E2_5678 0.26 -0.5 6,492,318 + Pf6N2E2_5678 0.26 +0.1 6,492,318 + Pf6N2E2_5678 0.26 +0.1 6,492,552 + Pf6N2E2_5678 0.72 +0.1 6,492,648 + +0.1 6,492,649 - +0.1 6,492,729 - +0.1 6,492,729 - +0.1 6,492,854 + +0.1 6,492,871 + Pf6N2E2_5679 0.13 +0.4 6,492,908 + Pf6N2E2_5679 0.39 -1.1 6,492,908 + Pf6N2E2_5679 0.39 +0.1 6,492,908 + Pf6N2E2_5679 0.39 -2.1 6,492,910 + Pf6N2E2_5679 0.40 +0.1 6,492,910 + Pf6N2E2_5679 0.40 -1.5 6,492,922 + Pf6N2E2_5679 0.49 +0.1 6,492,929 + Pf6N2E2_5679 0.54 +0.1 6,492,929 + Pf6N2E2_5679 0.54 +0.1 6,492,933 + +0.1 6,492,943 + Pf6N2E2_5679 0.64 +0.1 6,492,943 + Pf6N2E2_5679 0.64 -3.1 6,492,964 + Pf6N2E2_5679 0.79 +0.1 6,493,014 + +0.1
Or see this region's nucleotide sequence