Experiment: 10% P. fluorescens FW300-N2C3 spent media from growth in 20 mM putrescine
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt Pf6N2E2_5521 and Pf6N2E2_5522 are separated by 142 nucleotides Pf6N2E2_5522 and Pf6N2E2_5523 are separated by 152 nucleotides Pf6N2E2_5523 and Pf6N2E2_5524 are separated by 170 nucleotides
Pf6N2E2_5521 - 2-dehydropantoate 2-reductase (EC 1.1.1.169), at 6,340,919 to 6,341,836
_5521
Pf6N2E2_5522 - FIG001943: hypothetical protein YajQ, at 6,341,979 to 6,342,464
_5522
Pf6N2E2_5523 - Small-conductance mechanosensitive channel, at 6,342,617 to 6,343,459
_5523
Pf6N2E2_5524 - Outer membrane porin, OprD family, at 6,343,630 to 6,344,883
_5524
Position (kb)
6342
6343
6344 Strain fitness (log2 ratio)
-3
-2
-1
0
1
2 at 6341.845 kb on + strand at 6341.901 kb on + strand at 6341.901 kb on + strand at 6341.901 kb on + strand at 6341.903 kb on + strand at 6341.903 kb on + strand at 6341.903 kb on + strand at 6341.903 kb on + strand at 6341.903 kb on + strand at 6341.903 kb on + strand at 6341.903 kb on + strand at 6341.904 kb on - strand at 6341.904 kb on - strand at 6341.904 kb on - strand at 6341.904 kb on - strand at 6341.904 kb on - strand at 6341.904 kb on - strand at 6341.918 kb on + strand at 6341.946 kb on + strand at 6341.947 kb on - strand at 6341.976 kb on + strand at 6341.976 kb on + strand at 6341.976 kb on + strand at 6341.976 kb on + strand at 6341.976 kb on + strand at 6341.976 kb on + strand at 6341.976 kb on + strand at 6341.976 kb on + strand at 6341.976 kb on + strand at 6341.977 kb on - strand at 6341.977 kb on - strand at 6341.977 kb on - strand at 6341.977 kb on - strand at 6341.977 kb on - strand at 6341.977 kb on - strand at 6341.977 kb on - strand at 6341.977 kb on - strand at 6341.977 kb on - strand at 6341.977 kb on - strand at 6341.977 kb on - strand at 6341.977 kb on - strand at 6341.977 kb on - strand at 6341.999 kb on - strand at 6342.228 kb on + strand, within Pf6N2E2_5522 at 6342.229 kb on - strand, within Pf6N2E2_5522 at 6342.311 kb on + strand, within Pf6N2E2_5522 at 6342.311 kb on + strand, within Pf6N2E2_5522 at 6342.312 kb on - strand, within Pf6N2E2_5522 at 6342.312 kb on - strand, within Pf6N2E2_5522 at 6342.449 kb on + strand at 6342.465 kb on + strand at 6342.466 kb on - strand at 6342.466 kb on - strand at 6342.466 kb on - strand at 6342.523 kb on + strand at 6342.523 kb on + strand at 6342.523 kb on + strand at 6342.523 kb on + strand at 6342.523 kb on + strand at 6342.523 kb on + strand at 6342.523 kb on + strand at 6342.523 kb on + strand at 6342.523 kb on + strand at 6342.523 kb on + strand at 6342.523 kb on + strand at 6342.523 kb on + strand at 6342.523 kb on + strand at 6342.523 kb on + strand at 6342.523 kb on + strand at 6342.523 kb on + strand at 6342.523 kb on + strand at 6342.523 kb on + strand at 6342.523 kb on + strand at 6342.523 kb on + strand at 6342.524 kb on - strand at 6342.524 kb on - strand at 6342.524 kb on - strand at 6342.524 kb on - strand at 6342.524 kb on - strand at 6342.524 kb on - strand at 6342.525 kb on + strand at 6342.525 kb on + strand at 6342.526 kb on - strand at 6342.526 kb on - strand at 6342.590 kb on + strand at 6342.606 kb on - strand at 6342.606 kb on - strand at 6342.686 kb on + strand at 6342.844 kb on - strand, within Pf6N2E2_5523 at 6342.844 kb on - strand, within Pf6N2E2_5523 at 6342.844 kb on - strand, within Pf6N2E2_5523 at 6343.132 kb on + strand, within Pf6N2E2_5523 at 6343.187 kb on + strand, within Pf6N2E2_5523 at 6343.340 kb on + strand, within Pf6N2E2_5523 at 6343.341 kb on - strand, within Pf6N2E2_5523 at 6343.341 kb on - strand, within Pf6N2E2_5523 at 6343.405 kb on + strand at 6343.457 kb on + strand at 6343.469 kb on + strand at 6343.472 kb on - strand at 6343.515 kb on + strand at 6343.539 kb on + strand at 6343.539 kb on + strand at 6343.539 kb on + strand at 6343.540 kb on - strand at 6343.540 kb on - strand at 6343.553 kb on + strand at 6343.554 kb on - strand at 6343.620 kb on + strand at 6343.621 kb on - strand at 6343.621 kb on - strand at 6343.621 kb on - strand at 6343.632 kb on - strand at 6343.632 kb on - strand at 6343.649 kb on + strand at 6343.700 kb on + strand at 6343.700 kb on + strand at 6343.710 kb on + strand at 6343.710 kb on + strand at 6343.770 kb on + strand, within Pf6N2E2_5524 at 6343.771 kb on - strand, within Pf6N2E2_5524 at 6343.775 kb on + strand, within Pf6N2E2_5524 at 6343.775 kb on + strand, within Pf6N2E2_5524 at 6343.775 kb on + strand, within Pf6N2E2_5524 at 6343.775 kb on + strand, within Pf6N2E2_5524 at 6343.775 kb on + strand, within Pf6N2E2_5524 at 6343.775 kb on + strand, within Pf6N2E2_5524 at 6343.776 kb on - strand, within Pf6N2E2_5524 at 6343.776 kb on - strand, within Pf6N2E2_5524 at 6343.776 kb on - strand, within Pf6N2E2_5524 at 6343.776 kb on - strand, within Pf6N2E2_5524 at 6343.776 kb on - strand, within Pf6N2E2_5524 at 6343.776 kb on - strand, within Pf6N2E2_5524 at 6343.776 kb on - strand, within Pf6N2E2_5524 at 6343.776 kb on - strand, within Pf6N2E2_5524 at 6343.776 kb on - strand, within Pf6N2E2_5524 at 6343.847 kb on + strand, within Pf6N2E2_5524 at 6343.848 kb on - strand, within Pf6N2E2_5524 at 6343.880 kb on + strand, within Pf6N2E2_5524 at 6343.881 kb on - strand, within Pf6N2E2_5524 at 6343.881 kb on - strand, within Pf6N2E2_5524 at 6343.881 kb on - strand, within Pf6N2E2_5524 at 6343.881 kb on - strand, within Pf6N2E2_5524 at 6343.946 kb on + strand, within Pf6N2E2_5524 at 6343.994 kb on + strand, within Pf6N2E2_5524 at 6343.994 kb on + strand, within Pf6N2E2_5524 at 6343.994 kb on + strand, within Pf6N2E2_5524 at 6343.994 kb on + strand, within Pf6N2E2_5524 at 6343.994 kb on + strand, within Pf6N2E2_5524 at 6343.994 kb on + strand, within Pf6N2E2_5524 at 6343.994 kb on + strand, within Pf6N2E2_5524 at 6343.994 kb on + strand, within Pf6N2E2_5524 at 6343.995 kb on - strand, within Pf6N2E2_5524 at 6343.995 kb on - strand, within Pf6N2E2_5524 at 6344.000 kb on + strand, within Pf6N2E2_5524 at 6344.001 kb on - strand, within Pf6N2E2_5524 at 6344.060 kb on + strand, within Pf6N2E2_5524 at 6344.060 kb on + strand, within Pf6N2E2_5524 at 6344.060 kb on + strand, within Pf6N2E2_5524 at 6344.060 kb on + strand, within Pf6N2E2_5524 at 6344.060 kb on + strand, within Pf6N2E2_5524 at 6344.060 kb on + strand, within Pf6N2E2_5524 at 6344.060 kb on + strand, within Pf6N2E2_5524 at 6344.061 kb on - strand, within Pf6N2E2_5524 at 6344.061 kb on - strand, within Pf6N2E2_5524 at 6344.083 kb on - strand, within Pf6N2E2_5524 at 6344.083 kb on - strand, within Pf6N2E2_5524 at 6344.133 kb on - strand, within Pf6N2E2_5524 at 6344.183 kb on + strand, within Pf6N2E2_5524 at 6344.183 kb on + strand, within Pf6N2E2_5524 at 6344.183 kb on + strand, within Pf6N2E2_5524 at 6344.183 kb on + strand, within Pf6N2E2_5524 at 6344.183 kb on + strand, within Pf6N2E2_5524 at 6344.183 kb on + strand, within Pf6N2E2_5524 at 6344.184 kb on - strand, within Pf6N2E2_5524 at 6344.199 kb on - strand, within Pf6N2E2_5524 at 6344.199 kb on - strand, within Pf6N2E2_5524 at 6344.210 kb on + strand, within Pf6N2E2_5524 at 6344.210 kb on + strand, within Pf6N2E2_5524 at 6344.267 kb on + strand, within Pf6N2E2_5524 at 6344.267 kb on + strand, within Pf6N2E2_5524 at 6344.267 kb on + strand, within Pf6N2E2_5524 at 6344.267 kb on + strand, within Pf6N2E2_5524 at 6344.268 kb on - strand, within Pf6N2E2_5524
Per-strain Table
Position Strand Gene LocusTag Fraction 10% P. fluorescens FW300-N2C3 spent media from growth in 20 mM putrescine remove 6,341,845 + -0.2 6,341,901 + +0.1 6,341,901 + -0.9 6,341,901 + +0.1 6,341,903 + +0.1 6,341,903 + +0.1 6,341,903 + +0.1 6,341,903 + +0.1 6,341,903 + -2.1 6,341,903 + +0.1 6,341,903 + +0.1 6,341,904 - +0.1 6,341,904 - +2.4 6,341,904 - -2.7 6,341,904 - +0.1 6,341,904 - +0.1 6,341,904 - -0.5 6,341,918 + +0.6 6,341,946 + +0.1 6,341,947 - +0.1 6,341,976 + -0.3 6,341,976 + -0.1 6,341,976 + +0.1 6,341,976 + -0.6 6,341,976 + +0.2 6,341,976 + -1.0 6,341,976 + -0.7 6,341,976 + +0.1 6,341,976 + -1.5 6,341,977 - +0.5 6,341,977 - +1.1 6,341,977 - +0.7 6,341,977 - +0.1 6,341,977 - +0.1 6,341,977 - -1.3 6,341,977 - +2.4 6,341,977 - +0.7 6,341,977 - -0.9 6,341,977 - +0.1 6,341,977 - -0.9 6,341,977 - +1.1 6,341,977 - +0.1 6,341,999 - -0.9 6,342,228 + Pf6N2E2_5522 0.51 +0.1 6,342,229 - Pf6N2E2_5522 0.51 +1.3 6,342,311 + Pf6N2E2_5522 0.68 -0.6 6,342,311 + Pf6N2E2_5522 0.68 -0.4 6,342,312 - Pf6N2E2_5522 0.69 -1.2 6,342,312 - Pf6N2E2_5522 0.69 -0.9 6,342,449 + +0.1 6,342,465 + -0.1 6,342,466 - +0.9 6,342,466 - -0.7 6,342,466 - -0.1 6,342,523 + +0.7 6,342,523 + -0.6 6,342,523 + +0.7 6,342,523 + +0.1 6,342,523 + +0.1 6,342,523 + -0.6 6,342,523 + -0.7 6,342,523 + +0.1 6,342,523 + +0.4 6,342,523 + +0.4 6,342,523 + -3.1 6,342,523 + +0.3 6,342,523 + -0.9 6,342,523 + -0.1 6,342,523 + -0.6 6,342,523 + -0.2 6,342,523 + -2.9 6,342,523 + -1.9 6,342,523 + +0.1 6,342,523 + +0.4 6,342,524 - -2.5 6,342,524 - -0.6 6,342,524 - +0.1 6,342,524 - +0.7 6,342,524 - +0.1 6,342,524 - +0.1 6,342,525 + -0.4 6,342,525 + -0.5 6,342,526 - -0.9 6,342,526 - +0.7 6,342,590 + -0.1 6,342,606 - -1.5 6,342,606 - -0.1 6,342,686 + -1.3 6,342,844 - Pf6N2E2_5523 0.27 -1.9 6,342,844 - Pf6N2E2_5523 0.27 -0.0 6,342,844 - Pf6N2E2_5523 0.27 -1.1 6,343,132 + Pf6N2E2_5523 0.61 -0.6 6,343,187 + Pf6N2E2_5523 0.68 -0.1 6,343,340 + Pf6N2E2_5523 0.86 -0.9 6,343,341 - Pf6N2E2_5523 0.86 -0.9 6,343,341 - Pf6N2E2_5523 0.86 +0.1 6,343,405 + +0.1 6,343,457 + +0.9 6,343,469 + +1.7 6,343,472 - -0.9 6,343,515 + -0.1 6,343,539 + +0.1 6,343,539 + -2.5 6,343,539 + -1.8 6,343,540 - -1.7 6,343,540 - +0.1 6,343,553 + -0.8 6,343,554 - +0.1 6,343,620 + +1.4 6,343,621 - +0.1 6,343,621 - +0.1 6,343,621 - +0.1 6,343,632 - +0.1 6,343,632 - -0.9 6,343,649 + +0.1 6,343,700 + +0.1 6,343,700 + +0.1 6,343,710 + +0.1 6,343,710 + +0.1 6,343,770 + Pf6N2E2_5524 0.11 +0.1 6,343,771 - Pf6N2E2_5524 0.11 +0.1 6,343,775 + Pf6N2E2_5524 0.12 +0.1 6,343,775 + Pf6N2E2_5524 0.12 +0.1 6,343,775 + Pf6N2E2_5524 0.12 +0.1 6,343,775 + Pf6N2E2_5524 0.12 +0.1 6,343,775 + Pf6N2E2_5524 0.12 +0.1 6,343,775 + Pf6N2E2_5524 0.12 +0.1 6,343,776 - Pf6N2E2_5524 0.12 +0.1 6,343,776 - Pf6N2E2_5524 0.12 +0.1 6,343,776 - Pf6N2E2_5524 0.12 +0.1 6,343,776 - Pf6N2E2_5524 0.12 +0.1 6,343,776 - Pf6N2E2_5524 0.12 +1.7 6,343,776 - Pf6N2E2_5524 0.12 +0.1 6,343,776 - Pf6N2E2_5524 0.12 +0.1 6,343,776 - Pf6N2E2_5524 0.12 +0.1 6,343,776 - Pf6N2E2_5524 0.12 +0.1 6,343,847 + Pf6N2E2_5524 0.17 +0.1 6,343,848 - Pf6N2E2_5524 0.17 +0.1 6,343,880 + Pf6N2E2_5524 0.20 +0.1 6,343,881 - Pf6N2E2_5524 0.20 +0.1 6,343,881 - Pf6N2E2_5524 0.20 +0.1 6,343,881 - Pf6N2E2_5524 0.20 +0.1 6,343,881 - Pf6N2E2_5524 0.20 +0.1 6,343,946 + Pf6N2E2_5524 0.25 +0.1 6,343,994 + Pf6N2E2_5524 0.29 +0.1 6,343,994 + Pf6N2E2_5524 0.29 +0.1 6,343,994 + Pf6N2E2_5524 0.29 +0.1 6,343,994 + Pf6N2E2_5524 0.29 +0.1 6,343,994 + Pf6N2E2_5524 0.29 +0.1 6,343,994 + Pf6N2E2_5524 0.29 -0.1 6,343,994 + Pf6N2E2_5524 0.29 +0.1 6,343,994 + Pf6N2E2_5524 0.29 +0.1 6,343,995 - Pf6N2E2_5524 0.29 +0.1 6,343,995 - Pf6N2E2_5524 0.29 +0.1 6,344,000 + Pf6N2E2_5524 0.30 +0.1 6,344,001 - Pf6N2E2_5524 0.30 +0.1 6,344,060 + Pf6N2E2_5524 0.34 +0.1 6,344,060 + Pf6N2E2_5524 0.34 +0.7 6,344,060 + Pf6N2E2_5524 0.34 +0.1 6,344,060 + Pf6N2E2_5524 0.34 +0.1 6,344,060 + Pf6N2E2_5524 0.34 +0.1 6,344,060 + Pf6N2E2_5524 0.34 +0.1 6,344,060 + Pf6N2E2_5524 0.34 +0.1 6,344,061 - Pf6N2E2_5524 0.34 +0.1 6,344,061 - Pf6N2E2_5524 0.34 +0.1 6,344,083 - Pf6N2E2_5524 0.36 +0.1 6,344,083 - Pf6N2E2_5524 0.36 +0.1 6,344,133 - Pf6N2E2_5524 0.40 +0.1 6,344,183 + Pf6N2E2_5524 0.44 +0.1 6,344,183 + Pf6N2E2_5524 0.44 -0.5 6,344,183 + Pf6N2E2_5524 0.44 +0.1 6,344,183 + Pf6N2E2_5524 0.44 +0.1 6,344,183 + Pf6N2E2_5524 0.44 +0.1 6,344,183 + Pf6N2E2_5524 0.44 +0.1 6,344,184 - Pf6N2E2_5524 0.44 +0.1 6,344,199 - Pf6N2E2_5524 0.45 +0.1 6,344,199 - Pf6N2E2_5524 0.45 +0.1 6,344,210 + Pf6N2E2_5524 0.46 +0.1 6,344,210 + Pf6N2E2_5524 0.46 +0.1 6,344,267 + Pf6N2E2_5524 0.51 +2.4 6,344,267 + Pf6N2E2_5524 0.51 +0.1 6,344,267 + Pf6N2E2_5524 0.51 +0.1 6,344,267 + Pf6N2E2_5524 0.51 +0.1 6,344,268 - Pf6N2E2_5524 0.51 +0.1
Or see this region's nucleotide sequence