Strain Fitness in Pseudomonas fluorescens FW300-N2E2 around Pf6N2E2_54

Experiment: 10% P. fluorescens FW300-N2C3 spent media from growth in 20 mM putrescine

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Only strains with sufficient reads to estimate fitness are shown, but the strain fitness values are still rather noisy and may be biased towards zero. Strains near the edge of a gene are not shown as being associated with that gene (they are in grey). Strains in the central 10-90% of a gene are color coded by the insertion's strand. Usually, "+" means that the selectable marker is encoded on the forward strand, i.e., transcribed rightward.

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500 ntPf6N2E2_53 and Pf6N2E2_6096 are separated by 146 nucleotidesPf6N2E2_6096 and Pf6N2E2_6097 are separated by 82 nucleotidesPf6N2E2_6097 and Pf6N2E2_6098 are separated by 56 nucleotidesPf6N2E2_6098 and Pf6N2E2_6099 are separated by 78 nucleotidesPf6N2E2_6099 and Pf6N2E2_54 are separated by 184 nucleotidesPf6N2E2_54 and Pf6N2E2_55 are separated by 28 nucleotidesPf6N2E2_55 and Pf6N2E2_56 are separated by 45 nucleotides Pf6N2E2_53 - Glutamyl-tRNA synthetase (EC 6.1.1.17) @ Glutamyl-tRNA(Gln) synthetase (EC 6.1.1.24), at 92,556 to 94,037 _53 Pf6N2E2_6096 - tRNA-Ala-GGC, at 94,184 to 94,256 _6096 Pf6N2E2_6097 - tRNA-Glu-TTC, at 94,339 to 94,411 _6097 Pf6N2E2_6098 - tRNA-Ala-GGC, at 94,468 to 94,540 _6098 Pf6N2E2_6099 - tRNA-Glu-TTC, at 94,619 to 94,691 _6099 Pf6N2E2_54 - Transcriptional regulator, TetR family, at 94,876 to 95,415 _54 Pf6N2E2_55 - Probable hydrolase, at 95,444 to 96,277 _55 Pf6N2E2_56 - FIG002571: 4-hydroxybenzoyl-CoA thioesterase domain protein, at 96,323 to 96,772 _56 Position (kb) 94 95 96Strain fitness (log2 ratio) -2 -1 0 1 2at 94.263 kb on + strandat 94.263 kb on + strandat 94.263 kb on + strandat 94.324 kb on + strandat 94.546 kb on + strandat 94.546 kb on + strandat 94.663 kb on + strand, within Pf6N2E2_6099at 94.718 kb on + strandat 94.718 kb on + strandat 94.718 kb on + strandat 95.008 kb on + strand, within Pf6N2E2_54at 95.143 kb on + strand, within Pf6N2E2_54at 95.143 kb on + strand, within Pf6N2E2_54at 95.143 kb on + strand, within Pf6N2E2_54at 95.144 kb on - strand, within Pf6N2E2_54at 95.144 kb on - strand, within Pf6N2E2_54at 95.253 kb on + strand, within Pf6N2E2_54at 95.418 kb on + strandat 95.419 kb on - strandat 95.419 kb on - strandat 95.468 kb on - strandat 95.626 kb on - strand, within Pf6N2E2_55at 95.626 kb on - strand, within Pf6N2E2_55at 95.724 kb on - strand, within Pf6N2E2_55at 95.724 kb on - strand, within Pf6N2E2_55at 96.352 kb on + strandat 96.367 kb on - strandat 96.411 kb on + strand, within Pf6N2E2_56at 96.412 kb on - strand, within Pf6N2E2_56at 96.413 kb on + strand, within Pf6N2E2_56at 96.413 kb on + strand, within Pf6N2E2_56at 96.413 kb on + strand, within Pf6N2E2_56at 96.413 kb on + strand, within Pf6N2E2_56at 96.413 kb on + strand, within Pf6N2E2_56at 96.413 kb on + strand, within Pf6N2E2_56at 96.413 kb on + strand, within Pf6N2E2_56at 96.413 kb on + strand, within Pf6N2E2_56at 96.414 kb on - strand, within Pf6N2E2_56at 96.414 kb on - strandat 96.414 kb on - strand, within Pf6N2E2_56at 96.414 kb on - strand, within Pf6N2E2_56at 96.414 kb on - strand, within Pf6N2E2_56

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Per-strain Table

Position Strand Gene LocusTag Fraction 10% P. fluorescens FW300-N2C3 spent media from growth in 20 mM putrescine
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94,263 + -0.2
94,263 + +0.7
94,263 + +0.7
94,324 + +0.8
94,546 + -0.6
94,546 + -0.2
94,663 + Pf6N2E2_6099 0.60 +0.1
94,718 + +0.8
94,718 + +1.7
94,718 + -1.0
95,008 + Pf6N2E2_54 0.24 -2.3
95,143 + Pf6N2E2_54 0.49 -0.3
95,143 + Pf6N2E2_54 0.49 -0.2
95,143 + Pf6N2E2_54 0.49 -1.2
95,144 - Pf6N2E2_54 0.50 -0.2
95,144 - Pf6N2E2_54 0.50 -0.2
95,253 + Pf6N2E2_54 0.70 -0.3
95,418 + +0.5
95,419 - +0.8
95,419 - -0.7
95,468 - -0.2
95,626 - Pf6N2E2_55 0.22 +1.8
95,626 - Pf6N2E2_55 0.22 -0.2
95,724 - Pf6N2E2_55 0.34 -0.2
95,724 - Pf6N2E2_55 0.34 -0.9
96,352 + +0.2
96,367 - -0.7
96,411 + Pf6N2E2_56 0.20 +0.1
96,412 - Pf6N2E2_56 0.20 +2.0
96,413 + Pf6N2E2_56 0.20 +0.4
96,413 + Pf6N2E2_56 0.20 -0.4
96,413 + Pf6N2E2_56 0.20 -0.2
96,413 + Pf6N2E2_56 0.20 +0.6
96,413 + Pf6N2E2_56 0.20 -0.2
96,413 + Pf6N2E2_56 0.20 -0.2
96,413 + Pf6N2E2_56 0.20 -0.6
96,413 + Pf6N2E2_56 0.20 -0.2
96,414 - Pf6N2E2_56 0.20 -1.7
96,414 - +1.4
96,414 - Pf6N2E2_56 0.20 +0.5
96,414 - Pf6N2E2_56 0.20 +0.8
96,414 - Pf6N2E2_56 0.20 -0.2

Or see this region's nucleotide sequence