Experiment: 10% P. fluorescens FW300-N2C3 spent media from growth in 20 mM putrescine
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt Pf6N2E2_5335 and Pf6N2E2_5336 are separated by 14 nucleotides Pf6N2E2_5336 and Pf6N2E2_5337 are separated by 256 nucleotides Pf6N2E2_5337 and Pf6N2E2_5338 overlap by 1 nucleotides
Pf6N2E2_5335 - LSU ribosomal protein L13p (L13Ae), at 6,130,966 to 6,131,394
_5335
Pf6N2E2_5336 - SSU ribosomal protein S9p (S16e), at 6,131,409 to 6,131,801
_5336
Pf6N2E2_5337 - Ubiquinol-cytochrome C reductase iron-sulfur subunit (EC 1.10.2.2), at 6,132,058 to 6,132,651
_5337
Pf6N2E2_5338 - Ubiquinol--cytochrome c reductase, cytochrome B subunit (EC 1.10.2.2), at 6,132,651 to 6,133,862
_5338
Position (kb)
6132
6133 Strain fitness (log2 ratio)
-4
-3
-2
-1
0
1 at 6131.906 kb on + strand at 6131.908 kb on + strand at 6131.908 kb on + strand at 6131.908 kb on + strand at 6131.909 kb on - strand at 6131.909 kb on - strand at 6131.911 kb on + strand at 6131.911 kb on + strand at 6131.911 kb on + strand at 6131.912 kb on - strand at 6131.939 kb on + strand at 6131.939 kb on + strand at 6131.943 kb on + strand at 6131.943 kb on + strand at 6131.943 kb on + strand at 6131.944 kb on - strand at 6131.944 kb on - strand at 6131.944 kb on - strand at 6131.991 kb on + strand at 6131.992 kb on - strand at 6132.005 kb on + strand at 6132.009 kb on + strand at 6132.009 kb on + strand at 6132.010 kb on - strand at 6132.010 kb on - strand at 6132.012 kb on + strand at 6132.013 kb on - strand at 6132.013 kb on - strand at 6132.025 kb on + strand at 6132.025 kb on + strand at 6132.025 kb on + strand at 6132.370 kb on + strand, within Pf6N2E2_5337 at 6132.370 kb on + strand at 6132.370 kb on + strand, within Pf6N2E2_5337 at 6132.370 kb on + strand, within Pf6N2E2_5337 at 6132.370 kb on + strand, within Pf6N2E2_5337 at 6132.371 kb on - strand, within Pf6N2E2_5337 at 6132.371 kb on - strand, within Pf6N2E2_5337 at 6132.429 kb on + strand, within Pf6N2E2_5337 at 6132.430 kb on - strand, within Pf6N2E2_5337 at 6132.505 kb on + strand, within Pf6N2E2_5337 at 6132.506 kb on - strand, within Pf6N2E2_5337 at 6132.553 kb on + strand, within Pf6N2E2_5337 at 6132.553 kb on + strand, within Pf6N2E2_5337 at 6132.553 kb on + strand, within Pf6N2E2_5337 at 6132.554 kb on - strand at 6132.601 kb on + strand at 6132.602 kb on - strand at 6132.602 kb on - strand at 6132.723 kb on + strand at 6132.724 kb on - strand at 6132.727 kb on - strand at 6132.727 kb on - strand at 6132.753 kb on + strand at 6132.753 kb on + strand at 6132.825 kb on + strand, within Pf6N2E2_5338 at 6132.825 kb on + strand, within Pf6N2E2_5338 at 6132.825 kb on + strand, within Pf6N2E2_5338 at 6132.825 kb on + strand, within Pf6N2E2_5338 at 6132.825 kb on + strand, within Pf6N2E2_5338 at 6132.826 kb on - strand, within Pf6N2E2_5338 at 6132.826 kb on - strand, within Pf6N2E2_5338 at 6132.864 kb on + strand, within Pf6N2E2_5338 at 6132.864 kb on + strand, within Pf6N2E2_5338 at 6132.864 kb on + strand, within Pf6N2E2_5338 at 6132.864 kb on + strand, within Pf6N2E2_5338 at 6132.864 kb on + strand, within Pf6N2E2_5338 at 6132.865 kb on - strand, within Pf6N2E2_5338 at 6132.865 kb on - strand, within Pf6N2E2_5338 at 6132.972 kb on + strand, within Pf6N2E2_5338 at 6132.981 kb on + strand, within Pf6N2E2_5338 at 6132.981 kb on + strand, within Pf6N2E2_5338 at 6132.982 kb on - strand, within Pf6N2E2_5338 at 6133.060 kb on - strand, within Pf6N2E2_5338 at 6133.170 kb on + strand, within Pf6N2E2_5338 at 6133.171 kb on - strand, within Pf6N2E2_5338 at 6133.171 kb on - strand, within Pf6N2E2_5338 at 6133.365 kb on + strand, within Pf6N2E2_5338 at 6133.365 kb on + strand, within Pf6N2E2_5338 at 6133.365 kb on + strand, within Pf6N2E2_5338 at 6133.366 kb on - strand, within Pf6N2E2_5338 at 6133.368 kb on + strand, within Pf6N2E2_5338 at 6133.368 kb on + strand, within Pf6N2E2_5338 at 6133.382 kb on + strand, within Pf6N2E2_5338 at 6133.452 kb on + strand, within Pf6N2E2_5338 at 6133.452 kb on + strand, within Pf6N2E2_5338 at 6133.452 kb on + strand, within Pf6N2E2_5338 at 6133.453 kb on - strand, within Pf6N2E2_5338 at 6133.453 kb on - strand, within Pf6N2E2_5338 at 6133.453 kb on - strand, within Pf6N2E2_5338 at 6133.453 kb on - strand, within Pf6N2E2_5338 at 6133.453 kb on - strand, within Pf6N2E2_5338 at 6133.453 kb on - strand, within Pf6N2E2_5338 at 6133.524 kb on + strand, within Pf6N2E2_5338 at 6133.524 kb on + strand, within Pf6N2E2_5338 at 6133.540 kb on - strand, within Pf6N2E2_5338
Per-strain Table
Position Strand Gene LocusTag Fraction 10% P. fluorescens FW300-N2C3 spent media from growth in 20 mM putrescine remove 6,131,906 + +1.2 6,131,908 + +0.4 6,131,908 + +0.2 6,131,908 + -0.1 6,131,909 - +0.1 6,131,909 - +0.1 6,131,911 + -0.8 6,131,911 + +0.8 6,131,911 + -0.1 6,131,912 - -0.7 6,131,939 + +1.4 6,131,939 + -1.0 6,131,943 + -0.8 6,131,943 + +0.1 6,131,943 + -0.4 6,131,944 - -1.1 6,131,944 - -1.5 6,131,944 - -0.8 6,131,991 + +0.8 6,131,992 - -1.4 6,132,005 + -0.8 6,132,009 + -0.6 6,132,009 + -0.8 6,132,010 - -2.4 6,132,010 - -0.1 6,132,012 + +0.2 6,132,013 - -3.1 6,132,013 - +0.2 6,132,025 + +0.3 6,132,025 + -0.1 6,132,025 + +0.9 6,132,370 + Pf6N2E2_5337 0.53 -2.4 6,132,370 + -0.5 6,132,370 + Pf6N2E2_5337 0.53 -3.1 6,132,370 + Pf6N2E2_5337 0.53 -3.8 6,132,370 + Pf6N2E2_5337 0.53 -2.4 6,132,371 - Pf6N2E2_5337 0.53 -1.3 6,132,371 - Pf6N2E2_5337 0.53 -1.5 6,132,429 + Pf6N2E2_5337 0.62 -3.1 6,132,430 - Pf6N2E2_5337 0.63 -2.4 6,132,505 + Pf6N2E2_5337 0.75 -2.7 6,132,506 - Pf6N2E2_5337 0.75 -0.8 6,132,553 + Pf6N2E2_5337 0.83 -2.4 6,132,553 + Pf6N2E2_5337 0.83 -1.6 6,132,553 + Pf6N2E2_5337 0.83 -2.0 6,132,554 - -3.0 6,132,601 + -2.5 6,132,602 - -2.8 6,132,602 - -3.5 6,132,723 + +0.2 6,132,724 - -2.6 6,132,727 - +0.2 6,132,727 - +0.2 6,132,753 + -2.7 6,132,753 + -4.4 6,132,825 + Pf6N2E2_5338 0.14 -2.1 6,132,825 + Pf6N2E2_5338 0.14 -2.5 6,132,825 + Pf6N2E2_5338 0.14 -2.6 6,132,825 + Pf6N2E2_5338 0.14 -3.1 6,132,825 + Pf6N2E2_5338 0.14 -3.8 6,132,826 - Pf6N2E2_5338 0.14 -2.4 6,132,826 - Pf6N2E2_5338 0.14 -0.8 6,132,864 + Pf6N2E2_5338 0.18 -1.7 6,132,864 + Pf6N2E2_5338 0.18 -1.8 6,132,864 + Pf6N2E2_5338 0.18 -2.3 6,132,864 + Pf6N2E2_5338 0.18 -2.9 6,132,864 + Pf6N2E2_5338 0.18 -3.4 6,132,865 - Pf6N2E2_5338 0.18 -4.2 6,132,865 - Pf6N2E2_5338 0.18 -2.1 6,132,972 + Pf6N2E2_5338 0.26 -3.3 6,132,981 + Pf6N2E2_5338 0.27 -2.2 6,132,981 + Pf6N2E2_5338 0.27 -3.9 6,132,982 - Pf6N2E2_5338 0.27 -2.1 6,133,060 - Pf6N2E2_5338 0.34 -2.6 6,133,170 + Pf6N2E2_5338 0.43 -1.7 6,133,171 - Pf6N2E2_5338 0.43 -2.0 6,133,171 - Pf6N2E2_5338 0.43 -3.9 6,133,365 + Pf6N2E2_5338 0.59 -3.0 6,133,365 + Pf6N2E2_5338 0.59 -3.1 6,133,365 + Pf6N2E2_5338 0.59 -2.0 6,133,366 - Pf6N2E2_5338 0.59 -1.8 6,133,368 + Pf6N2E2_5338 0.59 -2.8 6,133,368 + Pf6N2E2_5338 0.59 -2.5 6,133,382 + Pf6N2E2_5338 0.60 -2.6 6,133,452 + Pf6N2E2_5338 0.66 -2.3 6,133,452 + Pf6N2E2_5338 0.66 -4.4 6,133,452 + Pf6N2E2_5338 0.66 -2.6 6,133,453 - Pf6N2E2_5338 0.66 +0.2 6,133,453 - Pf6N2E2_5338 0.66 -3.1 6,133,453 - Pf6N2E2_5338 0.66 -1.4 6,133,453 - Pf6N2E2_5338 0.66 -1.5 6,133,453 - Pf6N2E2_5338 0.66 -2.0 6,133,453 - Pf6N2E2_5338 0.66 -1.8 6,133,524 + Pf6N2E2_5338 0.72 -3.0 6,133,524 + Pf6N2E2_5338 0.72 -2.3 6,133,540 - Pf6N2E2_5338 0.73 -2.3
Or see this region's nucleotide sequence