Experiment: 10% P. fluorescens FW300-N2C3 spent media from growth in 20 mM putrescine
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt Pf6N2E2_5132 and Pf6N2E2_5133 are separated by 69 nucleotides Pf6N2E2_5133 and Pf6N2E2_5134 are separated by 52 nucleotides Pf6N2E2_5134 and Pf6N2E2_5135 are separated by 83 nucleotides
Pf6N2E2_5132 - NADH dehydrogenase (EC 1.6.99.3), at 5,918,438 to 5,919,736
_5132
Pf6N2E2_5133 - FIG00953980: hypothetical protein, at 5,919,806 to 5,919,979
_5133
Pf6N2E2_5134 - FIG060329: MOSC domain protein, at 5,920,032 to 5,920,472
_5134
Pf6N2E2_5135 - FIG00953333: hypothetical protein, at 5,920,556 to 5,921,182
_5135
Position (kb) 5919
5920 Strain fitness (log2 ratio) -1
0
1
2 at 5918.842 kb on + strand, within Pf6N2E2_5132 at 5918.843 kb on - strand, within Pf6N2E2_5132 at 5919.062 kb on - strand, within Pf6N2E2_5132 at 5919.062 kb on - strand, within Pf6N2E2_5132 at 5919.173 kb on - strand, within Pf6N2E2_5132 at 5919.173 kb on - strand, within Pf6N2E2_5132 at 5919.173 kb on - strand, within Pf6N2E2_5132 at 5919.273 kb on + strand, within Pf6N2E2_5132 at 5919.403 kb on - strand, within Pf6N2E2_5132 at 5919.425 kb on + strand, within Pf6N2E2_5132 at 5919.425 kb on + strand, within Pf6N2E2_5132 at 5919.426 kb on - strand, within Pf6N2E2_5132 at 5919.447 kb on + strand, within Pf6N2E2_5132 at 5919.447 kb on + strand, within Pf6N2E2_5132 at 5919.447 kb on + strand, within Pf6N2E2_5132 at 5919.448 kb on - strand, within Pf6N2E2_5132 at 5919.543 kb on + strand, within Pf6N2E2_5132 at 5919.543 kb on + strand, within Pf6N2E2_5132 at 5919.543 kb on + strand, within Pf6N2E2_5132 at 5919.543 kb on + strand, within Pf6N2E2_5132 at 5919.544 kb on - strand, within Pf6N2E2_5132 at 5919.544 kb on - strand, within Pf6N2E2_5132 at 5919.724 kb on + strand at 5919.724 kb on + strand at 5919.724 kb on + strand at 5919.724 kb on + strand at 5919.743 kb on - strand at 5919.809 kb on + strand at 5919.819 kb on + strand at 5919.820 kb on - strand at 5919.820 kb on - strand at 5919.933 kb on + strand, within Pf6N2E2_5133 at 5919.933 kb on + strand, within Pf6N2E2_5133 at 5919.934 kb on - strand, within Pf6N2E2_5133 at 5919.979 kb on + strand at 5919.980 kb on - strand at 5919.980 kb on - strand at 5919.980 kb on - strand at 5919.980 kb on - strand at 5920.020 kb on + strand at 5920.055 kb on - strand at 5920.117 kb on - strand, within Pf6N2E2_5134 at 5920.117 kb on - strand, within Pf6N2E2_5134 at 5920.163 kb on + strand, within Pf6N2E2_5134 at 5920.164 kb on - strand, within Pf6N2E2_5134 at 5920.164 kb on - strand, within Pf6N2E2_5134 at 5920.695 kb on + strand, within Pf6N2E2_5135 at 5920.696 kb on - strand, within Pf6N2E2_5135 at 5920.800 kb on + strand, within Pf6N2E2_5135 at 5920.801 kb on - strand, within Pf6N2E2_5135 at 5920.887 kb on - strand, within Pf6N2E2_5135 at 5920.887 kb on - strand, within Pf6N2E2_5135
Per-strain Table
Position Strand Gene LocusTag Fraction 10% P. fluorescens FW300-N2C3 spent media from growth in 20 mM putrescine remove 5,918,842 + Pf6N2E2_5132 0.31 +0.1 5,918,843 - Pf6N2E2_5132 0.31 -0.0 5,919,062 - Pf6N2E2_5132 0.48 -0.2 5,919,062 - Pf6N2E2_5132 0.48 -0.7 5,919,173 - Pf6N2E2_5132 0.57 +0.5 5,919,173 - Pf6N2E2_5132 0.57 +1.2 5,919,173 - Pf6N2E2_5132 0.57 +0.3 5,919,273 + Pf6N2E2_5132 0.64 +0.3 5,919,403 - Pf6N2E2_5132 0.74 -0.1 5,919,425 + Pf6N2E2_5132 0.76 +0.5 5,919,425 + Pf6N2E2_5132 0.76 -0.4 5,919,426 - Pf6N2E2_5132 0.76 -0.2 5,919,447 + Pf6N2E2_5132 0.78 +1.2 5,919,447 + Pf6N2E2_5132 0.78 +0.1 5,919,447 + Pf6N2E2_5132 0.78 +0.4 5,919,448 - Pf6N2E2_5132 0.78 -0.3 5,919,543 + Pf6N2E2_5132 0.85 +0.5 5,919,543 + Pf6N2E2_5132 0.85 -1.0 5,919,543 + Pf6N2E2_5132 0.85 -0.7 5,919,543 + Pf6N2E2_5132 0.85 -0.3 5,919,544 - Pf6N2E2_5132 0.85 +0.2 5,919,544 - Pf6N2E2_5132 0.85 -0.5 5,919,724 + -0.6 5,919,724 + -0.5 5,919,724 + +1.3 5,919,724 + +0.9 5,919,743 - +0.3 5,919,809 + -0.9 5,919,819 + +0.3 5,919,820 - +0.3 5,919,820 - +0.2 5,919,933 + Pf6N2E2_5133 0.73 -0.9 5,919,933 + Pf6N2E2_5133 0.73 -1.3 5,919,934 - Pf6N2E2_5133 0.74 -0.3 5,919,979 + -0.3 5,919,980 - +1.3 5,919,980 - -0.3 5,919,980 - +0.3 5,919,980 - +0.8 5,920,020 + -0.7 5,920,055 - -0.7 5,920,117 - Pf6N2E2_5134 0.19 -0.7 5,920,117 - Pf6N2E2_5134 0.19 +0.9 5,920,163 + Pf6N2E2_5134 0.30 +0.2 5,920,164 - Pf6N2E2_5134 0.30 +0.3 5,920,164 - Pf6N2E2_5134 0.30 +0.3 5,920,695 + Pf6N2E2_5135 0.22 +0.5 5,920,696 - Pf6N2E2_5135 0.22 +0.9 5,920,800 + Pf6N2E2_5135 0.39 +0.1 5,920,801 - Pf6N2E2_5135 0.39 +2.6 5,920,887 - Pf6N2E2_5135 0.53 +0.9 5,920,887 - Pf6N2E2_5135 0.53 +0.1
Or see this region's nucleotide sequence