Experiment: 10% P. fluorescens FW300-N2C3 spent media from growth in 20 mM putrescine
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt Pf6N2E2_4802 and Pf6N2E2_4803 are separated by 32 nucleotides Pf6N2E2_4803 and Pf6N2E2_4804 overlap by 1 nucleotides Pf6N2E2_4804 and Pf6N2E2_4805 are separated by 116 nucleotides Pf6N2E2_4805 and Pf6N2E2_4806 overlap by 4 nucleotides
Pf6N2E2_4802 - Thiosulfate sulfurtransferase GlpE (EC 2.8.1.1), at 5,552,896 to 5,553,225
_4802
Pf6N2E2_4803 - Bis(5'-nucleosyl)-tetraphosphatase, symmetrical (EC 3.6.1.41), at 5,553,258 to 5,554,133
_4803
Pf6N2E2_4804 - ApaG protein, at 5,554,133 to 5,554,513
_4804
Pf6N2E2_4805 - SSU rRNA (adenine(1518)-N(6)/adenine(1519)-N(6))- dimethyltransferase (EC 2.1.1.182), at 5,554,630 to 5,555,448
_4805
Pf6N2E2_4806 - 4-hydroxythreonine-4-phosphate dehydrogenase (EC 1.1.1.262), at 5,555,445 to 5,556,434
_4806
Position (kb) 5554
5555 Strain fitness (log2 ratio) -2
-1
0
1 at 5553.239 kb on - strand at 5553.239 kb on - strand at 5553.239 kb on - strand at 5553.239 kb on - strand at 5553.339 kb on + strand at 5553.339 kb on + strand at 5553.339 kb on + strand at 5553.339 kb on + strand at 5553.339 kb on + strand at 5553.339 kb on + strand at 5553.339 kb on + strand at 5553.339 kb on + strand at 5553.339 kb on + strand at 5553.339 kb on + strand at 5553.339 kb on + strand at 5553.339 kb on + strand at 5553.339 kb on + strand at 5553.339 kb on + strand at 5553.340 kb on - strand at 5553.340 kb on - strand at 5553.340 kb on - strand at 5553.340 kb on - strand at 5553.340 kb on - strand at 5553.340 kb on - strand at 5553.340 kb on - strand at 5553.595 kb on + strand, within Pf6N2E2_4803 at 5553.596 kb on - strand, within Pf6N2E2_4803 at 5553.664 kb on + strand, within Pf6N2E2_4803 at 5553.664 kb on + strand, within Pf6N2E2_4803 at 5554.124 kb on - strand at 5554.124 kb on - strand at 5554.135 kb on - strand at 5554.216 kb on - strand, within Pf6N2E2_4804 at 5554.216 kb on - strand, within Pf6N2E2_4804 at 5554.266 kb on + strand, within Pf6N2E2_4804 at 5554.267 kb on - strand, within Pf6N2E2_4804 at 5554.267 kb on - strand, within Pf6N2E2_4804 at 5554.267 kb on - strand, within Pf6N2E2_4804 at 5554.267 kb on - strand, within Pf6N2E2_4804 at 5554.267 kb on - strand, within Pf6N2E2_4804 at 5554.267 kb on - strand, within Pf6N2E2_4804 at 5554.411 kb on + strand, within Pf6N2E2_4804 at 5554.519 kb on + strand at 5554.519 kb on + strand at 5554.519 kb on + strand at 5554.519 kb on + strand at 5554.520 kb on - strand at 5554.520 kb on - strand at 5554.520 kb on - strand at 5554.520 kb on - strand at 5554.520 kb on - strand at 5554.520 kb on - strand at 5554.520 kb on - strand at 5554.520 kb on - strand at 5554.520 kb on - strand at 5554.527 kb on + strand at 5554.528 kb on - strand at 5554.569 kb on + strand at 5554.569 kb on + strand at 5554.570 kb on - strand at 5554.588 kb on + strand at 5554.588 kb on + strand at 5554.589 kb on - strand at 5554.604 kb on - strand at 5554.961 kb on + strand, within Pf6N2E2_4805 at 5555.112 kb on - strand, within Pf6N2E2_4805 at 5555.370 kb on + strand at 5555.435 kb on + strand
Per-strain Table
Position Strand Gene LocusTag Fraction 10% P. fluorescens FW300-N2C3 spent media from growth in 20 mM putrescine remove 5,553,239 - +0.4 5,553,239 - -0.1 5,553,239 - -0.6 5,553,239 - +0.1 5,553,339 + -0.4 5,553,339 + -1.2 5,553,339 + -2.0 5,553,339 + -0.6 5,553,339 + +1.0 5,553,339 + +0.8 5,553,339 + -0.1 5,553,339 + -0.3 5,553,339 + -1.2 5,553,339 + -1.6 5,553,339 + +0.2 5,553,339 + -1.4 5,553,339 + +0.9 5,553,339 + -1.6 5,553,340 - -0.3 5,553,340 - +0.4 5,553,340 - -1.1 5,553,340 - -0.4 5,553,340 - -0.6 5,553,340 - -0.9 5,553,340 - -0.6 5,553,595 + Pf6N2E2_4803 0.38 -1.9 5,553,596 - Pf6N2E2_4803 0.39 -0.6 5,553,664 + Pf6N2E2_4803 0.46 -1.9 5,553,664 + Pf6N2E2_4803 0.46 +0.4 5,554,124 - +0.0 5,554,124 - -0.2 5,554,135 - -1.6 5,554,216 - Pf6N2E2_4804 0.22 +0.4 5,554,216 - Pf6N2E2_4804 0.22 -0.4 5,554,266 + Pf6N2E2_4804 0.35 -2.6 5,554,267 - Pf6N2E2_4804 0.35 +0.2 5,554,267 - Pf6N2E2_4804 0.35 -1.1 5,554,267 - Pf6N2E2_4804 0.35 -1.1 5,554,267 - Pf6N2E2_4804 0.35 +0.1 5,554,267 - Pf6N2E2_4804 0.35 -1.9 5,554,267 - Pf6N2E2_4804 0.35 +0.4 5,554,411 + Pf6N2E2_4804 0.73 -1.6 5,554,519 + -0.9 5,554,519 + -2.4 5,554,519 + -1.4 5,554,519 + -1.2 5,554,520 - -0.0 5,554,520 - +0.4 5,554,520 - +0.4 5,554,520 - -1.2 5,554,520 - -0.3 5,554,520 - +0.1 5,554,520 - +0.4 5,554,520 - +0.4 5,554,520 - +0.4 5,554,527 + -1.2 5,554,528 - +0.1 5,554,569 + +0.4 5,554,569 + -0.2 5,554,570 - -0.3 5,554,588 + -0.8 5,554,588 + -0.1 5,554,589 - +0.4 5,554,604 - -0.6 5,554,961 + Pf6N2E2_4805 0.40 +0.4 5,555,112 - Pf6N2E2_4805 0.59 +0.4 5,555,370 + +0.4 5,555,435 + +0.4
Or see this region's nucleotide sequence