Strain Fitness in Pseudomonas fluorescens FW300-N2E2 around Pf6N2E2_477

Experiment: 10% P. fluorescens FW300-N2C3 spent media from growth in 20 mM putrescine

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Only strains with sufficient reads to estimate fitness are shown, but the strain fitness values are still rather noisy and may be biased towards zero. Strains near the edge of a gene are not shown as being associated with that gene (they are in grey). Strains in the central 10-90% of a gene are color coded by the insertion's strand. Usually, "+" means that the selectable marker is encoded on the forward strand, i.e., transcribed rightward.

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500 ntPf6N2E2_476 and Pf6N2E2_477 are separated by 175 nucleotidesPf6N2E2_477 and Pf6N2E2_478 are separated by 283 nucleotides Pf6N2E2_476 - Permease of the drug/metabolite transporter (DMT) superfamily, at 541,092 to 542,021 _476 Pf6N2E2_477 - Branched-chain amino acid aminotransferase (EC 2.6.1.42), at 542,197 to 543,216 _477 Pf6N2E2_478 - Dihydrolipoyl dehydrogenase (EC 1.8.1.4) (from data), at 543,500 to 544,885 _478 Position (kb) 542 543 544Strain fitness (log2 ratio) -2 -1 0 1at 541.373 kb on + strand, within Pf6N2E2_476at 541.373 kb on + strand, within Pf6N2E2_476at 541.405 kb on - strand, within Pf6N2E2_476at 541.615 kb on + strand, within Pf6N2E2_476at 541.616 kb on - strand, within Pf6N2E2_476at 541.919 kb on + strand, within Pf6N2E2_476at 542.019 kb on + strandat 542.264 kb on + strandat 542.327 kb on + strand, within Pf6N2E2_477at 542.327 kb on + strand, within Pf6N2E2_477at 542.328 kb on - strand, within Pf6N2E2_477at 542.328 kb on - strand, within Pf6N2E2_477at 542.328 kb on - strand, within Pf6N2E2_477at 542.652 kb on - strand, within Pf6N2E2_477at 542.747 kb on + strand, within Pf6N2E2_477at 542.843 kb on + strand, within Pf6N2E2_477at 543.173 kb on + strandat 543.174 kb on - strandat 543.174 kb on - strandat 543.226 kb on + strandat 543.226 kb on + strandat 543.227 kb on - strandat 543.227 kb on - strandat 543.288 kb on + strandat 543.288 kb on + strandat 543.288 kb on + strandat 543.288 kb on + strandat 543.288 kb on + strandat 543.288 kb on + strandat 543.289 kb on - strandat 543.289 kb on - strandat 543.289 kb on - strandat 543.289 kb on - strandat 543.289 kb on - strandat 543.289 kb on - strandat 543.289 kb on - strandat 543.289 kb on - strandat 543.289 kb on - strandat 543.501 kb on + strandat 544.195 kb on - strand, within Pf6N2E2_478at 544.195 kb on - strand, within Pf6N2E2_478

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Per-strain Table

Position Strand Gene LocusTag Fraction 10% P. fluorescens FW300-N2C3 spent media from growth in 20 mM putrescine
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541,373 + Pf6N2E2_476 0.30 +0.7
541,373 + Pf6N2E2_476 0.30 +1.3
541,405 - Pf6N2E2_476 0.34 +0.3
541,615 + Pf6N2E2_476 0.56 +0.7
541,616 - Pf6N2E2_476 0.56 +0.1
541,919 + Pf6N2E2_476 0.89 +0.7
542,019 + +0.1
542,264 + -0.7
542,327 + Pf6N2E2_477 0.13 -0.1
542,327 + Pf6N2E2_477 0.13 -1.3
542,328 - Pf6N2E2_477 0.13 -1.8
542,328 - Pf6N2E2_477 0.13 -1.2
542,328 - Pf6N2E2_477 0.13 -1.1
542,652 - Pf6N2E2_477 0.45 -0.3
542,747 + Pf6N2E2_477 0.54 -0.9
542,843 + Pf6N2E2_477 0.63 -1.3
543,173 + -0.4
543,174 - -2.7
543,174 - -1.1
543,226 + -1.2
543,226 + -1.3
543,227 - -0.9
543,227 - -1.3
543,288 + -0.3
543,288 + -0.7
543,288 + -0.7
543,288 + -0.6
543,288 + -0.3
543,288 + -0.2
543,289 - +0.4
543,289 - -0.3
543,289 - -0.0
543,289 - -1.0
543,289 - -0.6
543,289 - -0.1
543,289 - -0.3
543,289 - -0.3
543,289 - -0.2
543,501 + -1.6
544,195 - Pf6N2E2_478 0.50 -0.2
544,195 - Pf6N2E2_478 0.50 -0.0

Or see this region's nucleotide sequence