Experiment: 10% P. fluorescens FW300-N2C3 spent media from growth in 20 mM putrescine
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt Pf6N2E2_476 and Pf6N2E2_477 are separated by 175 nucleotides Pf6N2E2_477 and Pf6N2E2_478 are separated by 283 nucleotides
Pf6N2E2_476 - Permease of the drug/metabolite transporter (DMT) superfamily, at 541,092 to 542,021
_476
Pf6N2E2_477 - Branched-chain amino acid aminotransferase (EC 2.6.1.42), at 542,197 to 543,216
_477
Pf6N2E2_478 - Dihydrolipoyl dehydrogenase (EC 1.8.1.4) (from data) , at 543,500 to 544,885
_478
Position (kb)
542
543
544 Strain fitness (log2 ratio)
-2
-1
0
1 at 541.373 kb on + strand, within Pf6N2E2_476 at 541.373 kb on + strand, within Pf6N2E2_476 at 541.405 kb on - strand, within Pf6N2E2_476 at 541.615 kb on + strand, within Pf6N2E2_476 at 541.616 kb on - strand, within Pf6N2E2_476 at 541.919 kb on + strand, within Pf6N2E2_476 at 542.019 kb on + strand at 542.264 kb on + strand at 542.327 kb on + strand, within Pf6N2E2_477 at 542.327 kb on + strand, within Pf6N2E2_477 at 542.328 kb on - strand, within Pf6N2E2_477 at 542.328 kb on - strand, within Pf6N2E2_477 at 542.328 kb on - strand, within Pf6N2E2_477 at 542.652 kb on - strand, within Pf6N2E2_477 at 542.747 kb on + strand, within Pf6N2E2_477 at 542.843 kb on + strand, within Pf6N2E2_477 at 543.173 kb on + strand at 543.174 kb on - strand at 543.174 kb on - strand at 543.226 kb on + strand at 543.226 kb on + strand at 543.227 kb on - strand at 543.227 kb on - strand at 543.288 kb on + strand at 543.288 kb on + strand at 543.288 kb on + strand at 543.288 kb on + strand at 543.288 kb on + strand at 543.288 kb on + strand at 543.289 kb on - strand at 543.289 kb on - strand at 543.289 kb on - strand at 543.289 kb on - strand at 543.289 kb on - strand at 543.289 kb on - strand at 543.289 kb on - strand at 543.289 kb on - strand at 543.289 kb on - strand at 543.501 kb on + strand at 544.195 kb on - strand, within Pf6N2E2_478 at 544.195 kb on - strand, within Pf6N2E2_478
Per-strain Table
Position Strand Gene LocusTag Fraction 10% P. fluorescens FW300-N2C3 spent media from growth in 20 mM putrescine remove 541,373 + Pf6N2E2_476 0.30 +0.7 541,373 + Pf6N2E2_476 0.30 +1.3 541,405 - Pf6N2E2_476 0.34 +0.3 541,615 + Pf6N2E2_476 0.56 +0.7 541,616 - Pf6N2E2_476 0.56 +0.1 541,919 + Pf6N2E2_476 0.89 +0.7 542,019 + +0.1 542,264 + -0.7 542,327 + Pf6N2E2_477 0.13 -0.1 542,327 + Pf6N2E2_477 0.13 -1.3 542,328 - Pf6N2E2_477 0.13 -1.8 542,328 - Pf6N2E2_477 0.13 -1.2 542,328 - Pf6N2E2_477 0.13 -1.1 542,652 - Pf6N2E2_477 0.45 -0.3 542,747 + Pf6N2E2_477 0.54 -0.9 542,843 + Pf6N2E2_477 0.63 -1.3 543,173 + -0.4 543,174 - -2.7 543,174 - -1.1 543,226 + -1.2 543,226 + -1.3 543,227 - -0.9 543,227 - -1.3 543,288 + -0.3 543,288 + -0.7 543,288 + -0.7 543,288 + -0.6 543,288 + -0.3 543,288 + -0.2 543,289 - +0.4 543,289 - -0.3 543,289 - -0.0 543,289 - -1.0 543,289 - -0.6 543,289 - -0.1 543,289 - -0.3 543,289 - -0.3 543,289 - -0.2 543,501 + -1.6 544,195 - Pf6N2E2_478 0.50 -0.2 544,195 - Pf6N2E2_478 0.50 -0.0
Or see this region's nucleotide sequence