Experiment: 10% P. fluorescens FW300-N2C3 spent media from growth in 20 mM putrescine
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt Pf6N2E2_4515 and Pf6N2E2_4516 overlap by 4 nucleotides Pf6N2E2_4516 and Pf6N2E2_4517 overlap by 4 nucleotides Pf6N2E2_4517 and Pf6N2E2_4518 are separated by 235 nucleotides Pf6N2E2_4518 and Pf6N2E2_4519 are separated by 102 nucleotides
Pf6N2E2_4515 - Putrescine transport ATP-binding protein PotG (TC 3.A.1.11.2), at 5,248,124 to 5,249,266
_4515
Pf6N2E2_4516 - Putrescine transport system permease protein PotH (TC 3.A.1.11.2), at 5,249,263 to 5,250,183
_4516
Pf6N2E2_4517 - Putrescine transport system permease protein PotI (TC 3.A.1.11.2), at 5,250,180 to 5,251,067
_4517
Pf6N2E2_4518 - Carboxymuconolactone decarboxylase family protein, at 5,251,303 to 5,251,644
_4518
Pf6N2E2_4519 - Penicillin acylase II (EC 3.5.1.11), at 5,251,747 to 5,254,155
_4519
Position (kb) 5250
5251
5252 Strain fitness (log2 ratio) -4
-3
-2
-1
0
1
2 at 5249.254 kb on - strand at 5249.437 kb on + strand, within Pf6N2E2_4516 at 5249.437 kb on + strand, within Pf6N2E2_4516 at 5249.437 kb on + strand, within Pf6N2E2_4516 at 5249.437 kb on + strand, within Pf6N2E2_4516 at 5249.437 kb on + strand, within Pf6N2E2_4516 at 5249.437 kb on + strand, within Pf6N2E2_4516 at 5249.438 kb on - strand, within Pf6N2E2_4516 at 5249.438 kb on - strand, within Pf6N2E2_4516 at 5249.438 kb on - strand, within Pf6N2E2_4516 at 5249.438 kb on - strand, within Pf6N2E2_4516 at 5249.438 kb on - strand, within Pf6N2E2_4516 at 5249.443 kb on + strand, within Pf6N2E2_4516 at 5249.443 kb on + strand, within Pf6N2E2_4516 at 5249.443 kb on + strand, within Pf6N2E2_4516 at 5249.443 kb on + strand, within Pf6N2E2_4516 at 5249.443 kb on + strand, within Pf6N2E2_4516 at 5249.444 kb on - strand, within Pf6N2E2_4516 at 5249.444 kb on - strand, within Pf6N2E2_4516 at 5249.444 kb on - strand, within Pf6N2E2_4516 at 5249.444 kb on - strand, within Pf6N2E2_4516 at 5249.485 kb on + strand, within Pf6N2E2_4516 at 5249.485 kb on + strand, within Pf6N2E2_4516 at 5249.485 kb on + strand, within Pf6N2E2_4516 at 5249.512 kb on + strand, within Pf6N2E2_4516 at 5249.513 kb on - strand, within Pf6N2E2_4516 at 5249.513 kb on - strand, within Pf6N2E2_4516 at 5249.513 kb on - strand, within Pf6N2E2_4516 at 5249.594 kb on - strand, within Pf6N2E2_4516 at 5249.594 kb on - strand, within Pf6N2E2_4516 at 5249.594 kb on - strand, within Pf6N2E2_4516 at 5249.594 kb on - strand, within Pf6N2E2_4516 at 5249.748 kb on + strand, within Pf6N2E2_4516 at 5249.749 kb on - strand, within Pf6N2E2_4516 at 5249.791 kb on + strand, within Pf6N2E2_4516 at 5249.792 kb on - strand, within Pf6N2E2_4516 at 5249.792 kb on - strand, within Pf6N2E2_4516 at 5249.792 kb on - strand, within Pf6N2E2_4516 at 5249.792 kb on - strand, within Pf6N2E2_4516 at 5249.807 kb on - strand, within Pf6N2E2_4516 at 5249.812 kb on + strand, within Pf6N2E2_4516 at 5249.813 kb on - strand, within Pf6N2E2_4516 at 5249.840 kb on - strand, within Pf6N2E2_4516 at 5250.235 kb on - strand at 5250.261 kb on + strand at 5250.261 kb on + strand at 5250.261 kb on + strand at 5250.262 kb on - strand at 5250.319 kb on - strand, within Pf6N2E2_4517 at 5250.319 kb on - strand, within Pf6N2E2_4517 at 5250.385 kb on - strand, within Pf6N2E2_4517 at 5250.624 kb on + strand, within Pf6N2E2_4517 at 5250.625 kb on - strand, within Pf6N2E2_4517 at 5250.625 kb on - strand, within Pf6N2E2_4517 at 5250.625 kb on - strand, within Pf6N2E2_4517 at 5250.978 kb on - strand, within Pf6N2E2_4517 at 5251.066 kb on - strand at 5251.346 kb on + strand, within Pf6N2E2_4518 at 5251.346 kb on + strand, within Pf6N2E2_4518 at 5251.346 kb on + strand, within Pf6N2E2_4518 at 5251.346 kb on + strand, within Pf6N2E2_4518 at 5251.346 kb on + strand, within Pf6N2E2_4518 at 5251.346 kb on + strand, within Pf6N2E2_4518 at 5251.347 kb on - strand, within Pf6N2E2_4518 at 5251.350 kb on + strand, within Pf6N2E2_4518 at 5251.350 kb on + strand, within Pf6N2E2_4518 at 5251.351 kb on - strand, within Pf6N2E2_4518 at 5251.351 kb on - strand, within Pf6N2E2_4518 at 5251.351 kb on - strand, within Pf6N2E2_4518 at 5251.351 kb on - strand, within Pf6N2E2_4518 at 5251.352 kb on + strand, within Pf6N2E2_4518 at 5251.352 kb on + strand, within Pf6N2E2_4518 at 5251.352 kb on + strand, within Pf6N2E2_4518 at 5251.352 kb on + strand, within Pf6N2E2_4518 at 5251.353 kb on - strand, within Pf6N2E2_4518 at 5251.353 kb on - strand, within Pf6N2E2_4518 at 5251.353 kb on - strand, within Pf6N2E2_4518 at 5251.353 kb on - strand, within Pf6N2E2_4518 at 5251.353 kb on - strand, within Pf6N2E2_4518 at 5251.353 kb on - strand, within Pf6N2E2_4518 at 5251.353 kb on - strand, within Pf6N2E2_4518 at 5251.444 kb on + strand, within Pf6N2E2_4518 at 5251.445 kb on - strand, within Pf6N2E2_4518 at 5251.556 kb on + strand, within Pf6N2E2_4518 at 5251.556 kb on + strand, within Pf6N2E2_4518 at 5251.556 kb on + strand, within Pf6N2E2_4518 at 5251.557 kb on - strand, within Pf6N2E2_4518 at 5251.615 kb on - strand at 5251.615 kb on - strand at 5251.636 kb on - strand at 5251.636 kb on - strand at 5251.687 kb on + strand at 5251.687 kb on + strand at 5251.688 kb on - strand at 5251.688 kb on - strand at 5251.688 kb on - strand at 5251.722 kb on + strand at 5251.734 kb on - strand at 5251.771 kb on + strand at 5251.772 kb on - strand at 5251.832 kb on + strand at 5251.832 kb on + strand at 5251.832 kb on + strand at 5251.833 kb on - strand at 5251.847 kb on + strand at 5251.847 kb on + strand at 5251.847 kb on + strand at 5251.847 kb on + strand at 5251.848 kb on - strand at 5251.848 kb on - strand at 5251.848 kb on - strand at 5251.869 kb on - strand at 5251.956 kb on + strand at 5251.967 kb on + strand at 5251.967 kb on + strand at 5251.967 kb on + strand at 5251.967 kb on + strand at 5251.967 kb on + strand at 5251.967 kb on + strand at 5251.967 kb on + strand at 5251.967 kb on + strand at 5251.967 kb on + strand at 5251.967 kb on + strand at 5251.968 kb on - strand at 5251.968 kb on - strand at 5251.968 kb on - strand at 5251.968 kb on - strand at 5251.968 kb on - strand at 5251.968 kb on - strand at 5251.968 kb on - strand at 5251.968 kb on - strand at 5251.968 kb on - strand at 5251.968 kb on - strand at 5251.968 kb on - strand
Per-strain Table
Position Strand Gene LocusTag Fraction 10% P. fluorescens FW300-N2C3 spent media from growth in 20 mM putrescine remove 5,249,254 - -2.7 5,249,437 + Pf6N2E2_4516 0.19 -3.5 5,249,437 + Pf6N2E2_4516 0.19 -3.3 5,249,437 + Pf6N2E2_4516 0.19 -0.6 5,249,437 + Pf6N2E2_4516 0.19 -1.2 5,249,437 + Pf6N2E2_4516 0.19 -0.6 5,249,437 + Pf6N2E2_4516 0.19 -1.2 5,249,438 - Pf6N2E2_4516 0.19 -2.2 5,249,438 - Pf6N2E2_4516 0.19 -2.6 5,249,438 - Pf6N2E2_4516 0.19 -1.6 5,249,438 - Pf6N2E2_4516 0.19 -2.8 5,249,438 - Pf6N2E2_4516 0.19 -0.6 5,249,443 + Pf6N2E2_4516 0.20 +0.4 5,249,443 + Pf6N2E2_4516 0.20 -0.8 5,249,443 + Pf6N2E2_4516 0.20 -1.9 5,249,443 + Pf6N2E2_4516 0.20 -0.6 5,249,443 + Pf6N2E2_4516 0.20 -2.6 5,249,444 - Pf6N2E2_4516 0.20 -2.4 5,249,444 - Pf6N2E2_4516 0.20 -2.2 5,249,444 - Pf6N2E2_4516 0.20 -2.4 5,249,444 - Pf6N2E2_4516 0.20 -3.2 5,249,485 + Pf6N2E2_4516 0.24 -2.6 5,249,485 + Pf6N2E2_4516 0.24 -1.9 5,249,485 + Pf6N2E2_4516 0.24 +0.4 5,249,512 + Pf6N2E2_4516 0.27 +0.4 5,249,513 - Pf6N2E2_4516 0.27 -2.6 5,249,513 - Pf6N2E2_4516 0.27 -0.6 5,249,513 - Pf6N2E2_4516 0.27 -1.9 5,249,594 - Pf6N2E2_4516 0.36 -4.3 5,249,594 - Pf6N2E2_4516 0.36 -0.6 5,249,594 - Pf6N2E2_4516 0.36 -4.0 5,249,594 - Pf6N2E2_4516 0.36 -2.8 5,249,748 + Pf6N2E2_4516 0.53 -1.2 5,249,749 - Pf6N2E2_4516 0.53 -4.3 5,249,791 + Pf6N2E2_4516 0.57 -0.6 5,249,792 - Pf6N2E2_4516 0.57 -3.8 5,249,792 - Pf6N2E2_4516 0.57 -0.6 5,249,792 - Pf6N2E2_4516 0.57 +0.4 5,249,792 - Pf6N2E2_4516 0.57 -1.9 5,249,807 - Pf6N2E2_4516 0.59 -2.4 5,249,812 + Pf6N2E2_4516 0.60 -2.9 5,249,813 - Pf6N2E2_4516 0.60 +0.4 5,249,840 - Pf6N2E2_4516 0.63 -3.1 5,250,235 - -3.2 5,250,261 + -2.2 5,250,261 + -1.2 5,250,261 + -1.6 5,250,262 - +0.4 5,250,319 - Pf6N2E2_4517 0.16 -0.6 5,250,319 - Pf6N2E2_4517 0.16 -2.4 5,250,385 - Pf6N2E2_4517 0.23 +0.4 5,250,624 + Pf6N2E2_4517 0.50 +1.4 5,250,625 - Pf6N2E2_4517 0.50 -2.4 5,250,625 - Pf6N2E2_4517 0.50 -3.1 5,250,625 - Pf6N2E2_4517 0.50 +0.4 5,250,978 - Pf6N2E2_4517 0.90 +1.4 5,251,066 - +0.4 5,251,346 + Pf6N2E2_4518 0.13 -0.5 5,251,346 + Pf6N2E2_4518 0.13 +0.3 5,251,346 + Pf6N2E2_4518 0.13 +0.6 5,251,346 + Pf6N2E2_4518 0.13 +0.4 5,251,346 + Pf6N2E2_4518 0.13 +0.8 5,251,346 + Pf6N2E2_4518 0.13 +1.1 5,251,347 - Pf6N2E2_4518 0.13 +0.1 5,251,350 + Pf6N2E2_4518 0.14 -1.6 5,251,350 + Pf6N2E2_4518 0.14 -0.6 5,251,351 - Pf6N2E2_4518 0.14 -0.2 5,251,351 - Pf6N2E2_4518 0.14 -0.6 5,251,351 - Pf6N2E2_4518 0.14 -0.7 5,251,351 - Pf6N2E2_4518 0.14 -0.6 5,251,352 + Pf6N2E2_4518 0.14 -1.2 5,251,352 + Pf6N2E2_4518 0.14 +0.6 5,251,352 + Pf6N2E2_4518 0.14 -0.1 5,251,352 + Pf6N2E2_4518 0.14 -0.8 5,251,353 - Pf6N2E2_4518 0.15 -1.4 5,251,353 - Pf6N2E2_4518 0.15 +0.2 5,251,353 - Pf6N2E2_4518 0.15 +0.4 5,251,353 - Pf6N2E2_4518 0.15 -1.6 5,251,353 - Pf6N2E2_4518 0.15 +1.4 5,251,353 - Pf6N2E2_4518 0.15 -1.1 5,251,353 - Pf6N2E2_4518 0.15 -0.8 5,251,444 + Pf6N2E2_4518 0.41 -1.2 5,251,445 - Pf6N2E2_4518 0.42 +0.6 5,251,556 + Pf6N2E2_4518 0.74 +0.1 5,251,556 + Pf6N2E2_4518 0.74 -1.2 5,251,556 + Pf6N2E2_4518 0.74 -0.9 5,251,557 - Pf6N2E2_4518 0.74 -0.5 5,251,615 - +0.9 5,251,615 - +2.0 5,251,636 - +0.4 5,251,636 - -0.5 5,251,687 + -0.4 5,251,687 + +0.6 5,251,688 - +0.0 5,251,688 - -0.8 5,251,688 - -0.6 5,251,722 + +0.2 5,251,734 - +0.4 5,251,771 + -0.9 5,251,772 - -0.0 5,251,832 + -0.3 5,251,832 + +0.1 5,251,832 + -0.3 5,251,833 - -2.2 5,251,847 + -0.0 5,251,847 + +0.5 5,251,847 + -0.6 5,251,847 + -0.6 5,251,848 - -1.9 5,251,848 - +0.2 5,251,848 - -0.7 5,251,869 - -1.2 5,251,956 + +0.7 5,251,967 + -0.2 5,251,967 + -0.2 5,251,967 + -0.6 5,251,967 + -0.7 5,251,967 + +0.5 5,251,967 + -0.8 5,251,967 + +2.4 5,251,967 + +0.4 5,251,967 + -1.1 5,251,967 + -0.6 5,251,968 - +0.4 5,251,968 - -1.2 5,251,968 - -0.5 5,251,968 - +0.2 5,251,968 - -0.2 5,251,968 - -0.9 5,251,968 - +0.4 5,251,968 - +0.2 5,251,968 - +0.2 5,251,968 - +0.4 5,251,968 - +0.2
Or see this region's nucleotide sequence