Experiment: 10% P. fluorescens FW300-N2C3 spent media from growth in 20 mM putrescine
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt Pf6N2E2_4504 and Pf6N2E2_4505 are separated by 7 nucleotides Pf6N2E2_4505 and Pf6N2E2_4506 overlap by 4 nucleotides Pf6N2E2_4506 and Pf6N2E2_4507 are separated by 25 nucleotides Pf6N2E2_4507 and Pf6N2E2_4508 are separated by 185 nucleotides
Pf6N2E2_4504 - Alkanesulfonate monooxygenase (EC 1.14.14.5), at 5,236,121 to 5,237,269
_4504
Pf6N2E2_4505 - Alkanesulfonates transport system permease protein, at 5,237,277 to 5,238,059
_4505
Pf6N2E2_4506 - Alkanesulfonates ABC transporter ATP-binding protein / Sulfonate ABC transporter, ATP-binding subunit SsuB, at 5,238,056 to 5,238,862
_4506
Pf6N2E2_4507 - Organosulfonate utilization protein SsuF, at 5,238,888 to 5,239,103
_4507
Pf6N2E2_4508 - Transcriptional regulator, TetR family, at 5,239,289 to 5,239,921
_4508
Position (kb) 5238
5239 Strain fitness (log2 ratio) -2
-1
0
1 at 5237.075 kb on + strand, within Pf6N2E2_4504 at 5237.076 kb on - strand, within Pf6N2E2_4504 at 5237.135 kb on + strand, within Pf6N2E2_4504 at 5237.135 kb on + strand, within Pf6N2E2_4504 at 5237.136 kb on - strand, within Pf6N2E2_4504 at 5237.204 kb on + strand at 5237.204 kb on + strand at 5237.204 kb on + strand at 5237.204 kb on + strand at 5237.205 kb on - strand at 5237.205 kb on - strand at 5237.205 kb on - strand at 5237.205 kb on - strand at 5237.205 kb on - strand at 5237.239 kb on + strand at 5237.239 kb on + strand at 5237.240 kb on - strand at 5237.240 kb on - strand at 5237.324 kb on - strand at 5237.683 kb on - strand, within Pf6N2E2_4505 at 5237.694 kb on + strand, within Pf6N2E2_4505 at 5237.694 kb on + strand, within Pf6N2E2_4505 at 5237.694 kb on + strand, within Pf6N2E2_4505 at 5237.695 kb on - strand, within Pf6N2E2_4505 at 5237.695 kb on - strand, within Pf6N2E2_4505 at 5237.695 kb on - strand, within Pf6N2E2_4505 at 5237.695 kb on - strand, within Pf6N2E2_4505 at 5237.695 kb on - strand, within Pf6N2E2_4505 at 5237.745 kb on + strand, within Pf6N2E2_4505 at 5237.956 kb on - strand, within Pf6N2E2_4505 at 5238.031 kb on - strand at 5238.131 kb on - strand at 5238.217 kb on + strand, within Pf6N2E2_4506 at 5238.217 kb on + strand, within Pf6N2E2_4506 at 5238.218 kb on - strand, within Pf6N2E2_4506 at 5238.218 kb on - strand, within Pf6N2E2_4506 at 5238.218 kb on - strand, within Pf6N2E2_4506 at 5238.378 kb on + strand, within Pf6N2E2_4506 at 5238.378 kb on + strand, within Pf6N2E2_4506 at 5238.872 kb on + strand at 5238.872 kb on + strand at 5238.873 kb on - strand at 5238.873 kb on - strand at 5238.893 kb on + strand at 5238.894 kb on - strand at 5238.894 kb on - strand at 5239.089 kb on - strand at 5239.204 kb on + strand at 5239.205 kb on - strand at 5239.311 kb on + strand at 5239.311 kb on + strand at 5239.312 kb on - strand at 5239.323 kb on + strand at 5239.323 kb on + strand at 5239.323 kb on + strand at 5239.324 kb on - strand at 5239.324 kb on - strand at 5239.335 kb on + strand at 5239.335 kb on + strand at 5239.335 kb on + strand at 5239.336 kb on - strand at 5239.336 kb on - strand at 5239.398 kb on - strand, within Pf6N2E2_4508 at 5239.410 kb on + strand, within Pf6N2E2_4508 at 5239.605 kb on + strand, within Pf6N2E2_4508 at 5239.605 kb on + strand, within Pf6N2E2_4508 at 5239.605 kb on + strand, within Pf6N2E2_4508 at 5239.606 kb on - strand, within Pf6N2E2_4508 at 5239.606 kb on - strand, within Pf6N2E2_4508 at 5239.803 kb on + strand, within Pf6N2E2_4508 at 5239.804 kb on - strand, within Pf6N2E2_4508
Per-strain Table
Position Strand Gene LocusTag Fraction 10% P. fluorescens FW300-N2C3 spent media from growth in 20 mM putrescine remove 5,237,075 + Pf6N2E2_4504 0.83 -0.3 5,237,076 - Pf6N2E2_4504 0.83 +0.4 5,237,135 + Pf6N2E2_4504 0.88 +0.2 5,237,135 + Pf6N2E2_4504 0.88 -0.1 5,237,136 - Pf6N2E2_4504 0.88 -1.4 5,237,204 + +0.4 5,237,204 + -0.3 5,237,204 + +0.1 5,237,204 + +0.7 5,237,205 - +0.2 5,237,205 - +0.2 5,237,205 - -1.9 5,237,205 - -0.3 5,237,205 - +0.2 5,237,239 + +0.4 5,237,239 + +0.2 5,237,240 - +0.1 5,237,240 - +0.4 5,237,324 - +0.8 5,237,683 - Pf6N2E2_4505 0.52 +1.4 5,237,694 + Pf6N2E2_4505 0.53 -0.3 5,237,694 + Pf6N2E2_4505 0.53 -0.6 5,237,694 + Pf6N2E2_4505 0.53 -1.4 5,237,695 - Pf6N2E2_4505 0.53 -0.2 5,237,695 - Pf6N2E2_4505 0.53 +0.4 5,237,695 - Pf6N2E2_4505 0.53 +0.4 5,237,695 - Pf6N2E2_4505 0.53 +0.2 5,237,695 - Pf6N2E2_4505 0.53 +0.3 5,237,745 + Pf6N2E2_4505 0.60 -2.4 5,237,956 - Pf6N2E2_4505 0.87 -0.1 5,238,031 - -0.1 5,238,131 - +1.4 5,238,217 + Pf6N2E2_4506 0.20 -0.9 5,238,217 + Pf6N2E2_4506 0.20 -0.9 5,238,218 - Pf6N2E2_4506 0.20 -0.1 5,238,218 - Pf6N2E2_4506 0.20 -1.2 5,238,218 - Pf6N2E2_4506 0.20 +1.0 5,238,378 + Pf6N2E2_4506 0.40 +0.4 5,238,378 + Pf6N2E2_4506 0.40 -1.0 5,238,872 + -0.2 5,238,872 + -0.4 5,238,873 - -0.7 5,238,873 - +1.4 5,238,893 + +0.1 5,238,894 - -0.0 5,238,894 - -0.9 5,239,089 - +0.4 5,239,204 + -0.3 5,239,205 - -0.1 5,239,311 + -1.8 5,239,311 + +0.4 5,239,312 - +0.2 5,239,323 + +1.3 5,239,323 + -1.4 5,239,323 + -0.1 5,239,324 - -0.1 5,239,324 - +1.4 5,239,335 + +0.4 5,239,335 + -1.1 5,239,335 + -0.6 5,239,336 - +0.4 5,239,336 - -2.5 5,239,398 - Pf6N2E2_4508 0.17 +0.4 5,239,410 + Pf6N2E2_4508 0.19 -0.1 5,239,605 + Pf6N2E2_4508 0.50 +1.1 5,239,605 + Pf6N2E2_4508 0.50 -1.2 5,239,605 + Pf6N2E2_4508 0.50 -0.6 5,239,606 - Pf6N2E2_4508 0.50 +0.4 5,239,606 - Pf6N2E2_4508 0.50 +1.4 5,239,803 + Pf6N2E2_4508 0.81 -1.0 5,239,804 - Pf6N2E2_4508 0.81 -0.2
Or see this region's nucleotide sequence