Experiment: 10% P. fluorescens FW300-N2C3 spent media from growth in 20 mM putrescine
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt Pf6N2E2_405 and Pf6N2E2_406 are separated by 341 nucleotides
Pf6N2E2_405 - hypothetical protein, at 475,529 to 475,801
_405
Pf6N2E2_406 - hypothetical protein, at 476,143 to 477,102
_406
Position (kb) 475
476 Strain fitness (log2 ratio) -2
-1
0
1
2 at 474.627 kb on + strand at 474.627 kb on + strand at 474.834 kb on + strand at 474.835 kb on - strand at 474.835 kb on - strand at 474.840 kb on + strand at 474.930 kb on + strand at 474.973 kb on - strand at 475.054 kb on + strand at 475.134 kb on + strand at 475.134 kb on + strand at 475.134 kb on + strand at 475.135 kb on - strand at 475.136 kb on + strand at 475.176 kb on + strand at 475.188 kb on + strand at 475.188 kb on + strand at 475.189 kb on - strand at 475.189 kb on - strand at 475.219 kb on - strand at 475.245 kb on + strand at 475.245 kb on + strand at 475.269 kb on + strand at 475.269 kb on + strand at 475.270 kb on - strand at 475.270 kb on - strand at 475.270 kb on - strand at 475.345 kb on - strand at 475.414 kb on + strand at 475.414 kb on + strand at 475.414 kb on + strand at 475.415 kb on - strand at 475.415 kb on - strand at 475.415 kb on - strand at 475.480 kb on + strand at 475.480 kb on + strand at 475.480 kb on + strand at 475.480 kb on + strand at 475.480 kb on + strand at 475.481 kb on - strand at 475.481 kb on - strand at 475.481 kb on - strand at 475.530 kb on + strand at 475.531 kb on - strand at 475.676 kb on + strand, within Pf6N2E2_405 at 475.677 kb on - strand, within Pf6N2E2_405 at 475.791 kb on + strand at 475.791 kb on + strand at 475.791 kb on + strand at 475.791 kb on + strand at 475.791 kb on + strand at 475.791 kb on + strand at 475.792 kb on - strand at 475.792 kb on - strand at 475.792 kb on - strand at 475.792 kb on - strand at 475.792 kb on - strand at 475.792 kb on - strand at 475.792 kb on - strand at 475.792 kb on - strand at 475.792 kb on - strand at 475.792 kb on - strand at 475.884 kb on - strand at 475.885 kb on + strand at 475.885 kb on + strand at 475.886 kb on - strand at 475.986 kb on - strand at 475.986 kb on - strand at 475.995 kb on + strand at 475.996 kb on - strand at 476.151 kb on - strand at 476.151 kb on - strand at 476.151 kb on - strand at 476.235 kb on - strand at 476.250 kb on + strand, within Pf6N2E2_406 at 476.251 kb on - strand, within Pf6N2E2_406 at 476.251 kb on - strand, within Pf6N2E2_406 at 476.330 kb on + strand, within Pf6N2E2_406 at 476.331 kb on - strand, within Pf6N2E2_406 at 476.331 kb on - strand, within Pf6N2E2_406 at 476.386 kb on + strand, within Pf6N2E2_406 at 476.386 kb on + strand, within Pf6N2E2_406 at 476.386 kb on + strand, within Pf6N2E2_406 at 476.386 kb on + strand, within Pf6N2E2_406 at 476.386 kb on + strand, within Pf6N2E2_406 at 476.386 kb on + strand, within Pf6N2E2_406 at 476.568 kb on - strand, within Pf6N2E2_406 at 476.728 kb on + strand, within Pf6N2E2_406 at 476.728 kb on + strand, within Pf6N2E2_406 at 476.730 kb on + strand, within Pf6N2E2_406 at 476.730 kb on + strand, within Pf6N2E2_406 at 476.731 kb on - strand, within Pf6N2E2_406 at 476.748 kb on + strand, within Pf6N2E2_406 at 476.748 kb on + strand, within Pf6N2E2_406
Per-strain Table
Position Strand Gene LocusTag Fraction 10% P. fluorescens FW300-N2C3 spent media from growth in 20 mM putrescine remove 474,627 + +0.7 474,627 + -1.9 474,834 + -0.6 474,835 - +2.3 474,835 - -0.3 474,840 + +0.5 474,930 + +0.4 474,973 - -0.3 475,054 + -0.3 475,134 + +0.9 475,134 + -0.3 475,134 + -0.6 475,135 - +0.8 475,136 + -0.8 475,176 + -1.9 475,188 + -0.6 475,188 + -1.6 475,189 - -1.2 475,189 - +0.9 475,219 - +0.4 475,245 + -0.3 475,245 + +0.7 475,269 + -0.3 475,269 + -0.3 475,270 - +1.3 475,270 - -0.3 475,270 - +1.0 475,345 - +1.1 475,414 + +0.7 475,414 + -0.7 475,414 + +0.2 475,415 - +0.3 475,415 - +0.4 475,415 - +0.7 475,480 + +0.9 475,480 + -0.3 475,480 + -0.3 475,480 + +0.7 475,480 + +2.3 475,481 - +1.3 475,481 - +0.1 475,481 - -0.0 475,530 + +0.2 475,531 - -0.6 475,676 + Pf6N2E2_405 0.54 -1.9 475,677 - Pf6N2E2_405 0.54 -1.3 475,791 + -0.6 475,791 + -1.0 475,791 + -0.4 475,791 + +0.3 475,791 + +0.5 475,791 + +1.3 475,792 - -0.5 475,792 - -0.1 475,792 - +0.4 475,792 - -0.8 475,792 - +0.3 475,792 - +1.4 475,792 - +2.0 475,792 - +0.7 475,792 - -0.3 475,792 - -0.0 475,884 - +0.1 475,885 + -0.7 475,885 + -0.3 475,886 - +0.1 475,986 - -0.3 475,986 - +0.6 475,995 + +0.2 475,996 - +0.7 476,151 - -0.1 476,151 - -0.3 476,151 - +0.4 476,235 - +0.1 476,250 + Pf6N2E2_406 0.11 -0.1 476,251 - Pf6N2E2_406 0.11 +0.7 476,251 - Pf6N2E2_406 0.11 +0.7 476,330 + Pf6N2E2_406 0.19 -0.6 476,331 - Pf6N2E2_406 0.20 -1.3 476,331 - Pf6N2E2_406 0.20 -0.1 476,386 + Pf6N2E2_406 0.25 -0.8 476,386 + Pf6N2E2_406 0.25 -0.2 476,386 + Pf6N2E2_406 0.25 -0.3 476,386 + Pf6N2E2_406 0.25 +0.3 476,386 + Pf6N2E2_406 0.25 +0.0 476,386 + Pf6N2E2_406 0.25 -0.3 476,568 - Pf6N2E2_406 0.44 -1.3 476,728 + Pf6N2E2_406 0.61 +0.2 476,728 + Pf6N2E2_406 0.61 -0.3 476,730 + Pf6N2E2_406 0.61 -0.3 476,730 + Pf6N2E2_406 0.61 +1.9 476,731 - Pf6N2E2_406 0.61 +0.7 476,748 + Pf6N2E2_406 0.63 -0.1 476,748 + Pf6N2E2_406 0.63 -0.8
Or see this region's nucleotide sequence