Experiment: 10% P. fluorescens FW300-N2C3 spent media from growth in 20 mM putrescine
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt Pf6N2E2_372 and Pf6N2E2_373 are separated by 288 nucleotides Pf6N2E2_373 and Pf6N2E2_374 are separated by 87 nucleotides Pf6N2E2_374 and Pf6N2E2_375 are separated by 45 nucleotides
Pf6N2E2_372 - D-3-phosphoglycerate dehydrogenase (EC 1.1.1.95), at 439,581 to 440,507
_372
Pf6N2E2_373 - Gamma-aminobutyrate:alpha-ketoglutarate aminotransferase (EC 2.6.1.19), at 440,796 to 442,088
_373
Pf6N2E2_374 - FIG00953249: hypothetical protein, at 442,176 to 442,832
_374
Pf6N2E2_375 - Leucine-responsive regulatory protein, regulator for leucine (or lrp) regulon and high-affinity branched-chain amino acid transport system, at 442,878 to 443,366
_375
Position (kb) 440
441
442
443 Strain fitness (log2 ratio) -1
0
1 at 439.938 kb on + strand, within Pf6N2E2_372 at 439.938 kb on + strand, within Pf6N2E2_372 at 439.938 kb on + strand, within Pf6N2E2_372 at 439.939 kb on - strand, within Pf6N2E2_372 at 439.939 kb on - strand, within Pf6N2E2_372 at 439.939 kb on - strand, within Pf6N2E2_372 at 439.939 kb on - strand, within Pf6N2E2_372 at 440.067 kb on + strand, within Pf6N2E2_372 at 440.215 kb on + strand, within Pf6N2E2_372 at 440.274 kb on - strand, within Pf6N2E2_372 at 440.384 kb on - strand, within Pf6N2E2_372 at 440.384 kb on - strand, within Pf6N2E2_372 at 440.384 kb on - strand, within Pf6N2E2_372 at 440.384 kb on - strand, within Pf6N2E2_372 at 440.492 kb on + strand at 440.494 kb on + strand at 440.494 kb on + strand at 440.495 kb on - strand at 440.495 kb on - strand at 440.495 kb on - strand at 440.540 kb on + strand at 440.586 kb on - strand at 440.676 kb on + strand at 440.677 kb on - strand at 440.912 kb on - strand at 441.063 kb on + strand, within Pf6N2E2_373 at 441.114 kb on + strand, within Pf6N2E2_373 at 441.115 kb on - strand, within Pf6N2E2_373 at 441.117 kb on - strand, within Pf6N2E2_373 at 441.117 kb on - strand, within Pf6N2E2_373 at 441.270 kb on + strand, within Pf6N2E2_373 at 441.271 kb on - strand, within Pf6N2E2_373 at 441.330 kb on + strand, within Pf6N2E2_373 at 441.330 kb on + strand, within Pf6N2E2_373 at 441.699 kb on + strand, within Pf6N2E2_373 at 441.700 kb on - strand, within Pf6N2E2_373 at 442.133 kb on + strand at 442.327 kb on + strand, within Pf6N2E2_374 at 442.328 kb on - strand, within Pf6N2E2_374 at 442.341 kb on + strand, within Pf6N2E2_374 at 442.503 kb on + strand, within Pf6N2E2_374 at 442.503 kb on + strand, within Pf6N2E2_374 at 442.503 kb on + strand, within Pf6N2E2_374 at 442.504 kb on - strand, within Pf6N2E2_374 at 442.774 kb on - strand at 442.774 kb on - strand at 442.839 kb on + strand at 442.853 kb on - strand at 442.853 kb on - strand at 443.043 kb on + strand, within Pf6N2E2_375 at 443.043 kb on + strand, within Pf6N2E2_375
Per-strain Table
Position Strand Gene LocusTag Fraction 10% P. fluorescens FW300-N2C3 spent media from growth in 20 mM putrescine remove 439,938 + Pf6N2E2_372 0.39 +1.1 439,938 + Pf6N2E2_372 0.39 -0.1 439,938 + Pf6N2E2_372 0.39 +0.2 439,939 - Pf6N2E2_372 0.39 +1.1 439,939 - Pf6N2E2_372 0.39 +0.3 439,939 - Pf6N2E2_372 0.39 -0.3 439,939 - Pf6N2E2_372 0.39 -0.7 440,067 + Pf6N2E2_372 0.52 +0.4 440,215 + Pf6N2E2_372 0.68 +0.4 440,274 - Pf6N2E2_372 0.75 -0.5 440,384 - Pf6N2E2_372 0.87 +0.1 440,384 - Pf6N2E2_372 0.87 +1.3 440,384 - Pf6N2E2_372 0.87 +0.0 440,384 - Pf6N2E2_372 0.87 +0.3 440,492 + -0.6 440,494 + +0.5 440,494 + +0.8 440,495 - +0.3 440,495 - +0.1 440,495 - -0.3 440,540 + +1.0 440,586 - -0.7 440,676 + -0.6 440,677 - +0.7 440,912 - -1.1 441,063 + Pf6N2E2_373 0.21 -0.9 441,114 + Pf6N2E2_373 0.25 -0.3 441,115 - Pf6N2E2_373 0.25 -0.0 441,117 - Pf6N2E2_373 0.25 +0.1 441,117 - Pf6N2E2_373 0.25 -0.7 441,270 + Pf6N2E2_373 0.37 +0.2 441,271 - Pf6N2E2_373 0.37 +1.1 441,330 + Pf6N2E2_373 0.41 +0.5 441,330 + Pf6N2E2_373 0.41 -0.8 441,699 + Pf6N2E2_373 0.70 -1.6 441,700 - Pf6N2E2_373 0.70 -0.3 442,133 + +0.1 442,327 + Pf6N2E2_374 0.23 +0.1 442,328 - Pf6N2E2_374 0.23 -0.3 442,341 + Pf6N2E2_374 0.25 -0.7 442,503 + Pf6N2E2_374 0.50 -0.7 442,503 + Pf6N2E2_374 0.50 -0.3 442,503 + Pf6N2E2_374 0.50 -0.3 442,504 - Pf6N2E2_374 0.50 -0.6 442,774 - -1.3 442,774 - +0.2 442,839 + +0.7 442,853 - +0.2 442,853 - -0.7 443,043 + Pf6N2E2_375 0.34 +0.1 443,043 + Pf6N2E2_375 0.34 +0.2
Or see this region's nucleotide sequence