Experiment: 10% P. fluorescens FW300-N2C3 spent media from growth in 20 mM putrescine
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt Pf6N2E2_367 and Pf6N2E2_368 are separated by 254 nucleotides Pf6N2E2_368 and Pf6N2E2_369 are separated by 40 nucleotides
Pf6N2E2_367 - Acetylornithine deacetylase (EC 3.5.1.16), at 433,628 to 434,785
_367
Pf6N2E2_368 - Succinate-semialdehyde dehydrogenase [NAD(P)+] (EC 1.2.1.16), at 435,040 to 436,497
_368
Pf6N2E2_369 - Tartrate dehydrogenase (EC 1.1.1.93) @ Tartrate decarboxylase (EC 4.1.1.73) @ D-malic enzyme (EC 1.1.1.83), at 436,538 to 437,617
_369
Position (kb) 435
436
437 Strain fitness (log2 ratio) -1
0
1
2 at 434.318 kb on + strand, within Pf6N2E2_367 at 434.528 kb on + strand, within Pf6N2E2_367 at 434.783 kb on + strand at 434.792 kb on + strand at 434.792 kb on + strand at 434.792 kb on + strand at 434.792 kb on + strand at 434.792 kb on + strand at 434.792 kb on + strand at 434.793 kb on - strand at 434.793 kb on - strand at 434.793 kb on - strand at 434.793 kb on - strand at 434.793 kb on - strand at 434.800 kb on + strand at 434.800 kb on + strand at 434.800 kb on + strand at 434.801 kb on - strand at 434.801 kb on - strand at 434.801 kb on - strand at 434.893 kb on + strand at 434.893 kb on + strand at 434.893 kb on + strand at 434.894 kb on - strand at 434.894 kb on - strand at 434.894 kb on - strand at 434.894 kb on - strand at 434.894 kb on - strand at 434.894 kb on - strand at 435.007 kb on + strand at 435.018 kb on + strand at 435.088 kb on + strand at 435.089 kb on - strand at 435.174 kb on - strand at 435.427 kb on + strand, within Pf6N2E2_368 at 435.952 kb on + strand, within Pf6N2E2_368 at 435.952 kb on + strand, within Pf6N2E2_368 at 435.952 kb on + strand, within Pf6N2E2_368 at 435.953 kb on - strand, within Pf6N2E2_368 at 436.236 kb on + strand, within Pf6N2E2_368 at 436.239 kb on + strand, within Pf6N2E2_368 at 436.295 kb on - strand, within Pf6N2E2_368 at 436.297 kb on + strand, within Pf6N2E2_368 at 436.297 kb on + strand, within Pf6N2E2_368 at 436.444 kb on + strand at 436.445 kb on - strand at 436.445 kb on - strand at 436.445 kb on - strand at 436.459 kb on + strand at 436.686 kb on - strand, within Pf6N2E2_369 at 436.688 kb on + strand, within Pf6N2E2_369 at 436.688 kb on + strand, within Pf6N2E2_369 at 436.688 kb on + strand, within Pf6N2E2_369 at 436.688 kb on + strand, within Pf6N2E2_369 at 436.688 kb on + strand, within Pf6N2E2_369 at 436.688 kb on + strand, within Pf6N2E2_369 at 436.689 kb on - strand, within Pf6N2E2_369 at 436.754 kb on + strand, within Pf6N2E2_369 at 436.844 kb on + strand, within Pf6N2E2_369 at 436.844 kb on + strand, within Pf6N2E2_369 at 436.845 kb on - strand, within Pf6N2E2_369 at 436.955 kb on + strand, within Pf6N2E2_369 at 437.405 kb on + strand, within Pf6N2E2_369 at 437.405 kb on + strand, within Pf6N2E2_369 at 437.405 kb on + strand, within Pf6N2E2_369 at 437.405 kb on + strand, within Pf6N2E2_369 at 437.406 kb on - strand, within Pf6N2E2_369 at 437.490 kb on - strand, within Pf6N2E2_369
Per-strain Table
Position Strand Gene LocusTag Fraction 10% P. fluorescens FW300-N2C3 spent media from growth in 20 mM putrescine remove 434,318 + Pf6N2E2_367 0.60 +0.7 434,528 + Pf6N2E2_367 0.78 +1.6 434,783 + -1.3 434,792 + -0.4 434,792 + +0.9 434,792 + +0.2 434,792 + -0.7 434,792 + +0.5 434,792 + -0.1 434,793 - -0.3 434,793 - +0.4 434,793 - -0.8 434,793 - +0.5 434,793 - +0.3 434,800 + -0.5 434,800 + -0.3 434,800 + -0.0 434,801 - +0.7 434,801 - +0.1 434,801 - -0.3 434,893 + -0.1 434,893 + -0.8 434,893 + +0.0 434,894 - +0.4 434,894 - +0.8 434,894 - -0.3 434,894 - -0.7 434,894 - +1.1 434,894 - -0.1 435,007 + -0.2 435,018 + +0.3 435,088 + +0.3 435,089 - +0.0 435,174 - +2.7 435,427 + Pf6N2E2_368 0.27 -0.3 435,952 + Pf6N2E2_368 0.63 -1.3 435,952 + Pf6N2E2_368 0.63 -0.3 435,952 + Pf6N2E2_368 0.63 +0.3 435,953 - Pf6N2E2_368 0.63 -1.3 436,236 + Pf6N2E2_368 0.82 +0.1 436,239 + Pf6N2E2_368 0.82 +0.1 436,295 - Pf6N2E2_368 0.86 -1.3 436,297 + Pf6N2E2_368 0.86 +0.8 436,297 + Pf6N2E2_368 0.86 -1.0 436,444 + -0.4 436,445 - -1.6 436,445 - -1.1 436,445 - -1.3 436,459 + -1.6 436,686 - Pf6N2E2_369 0.14 -0.3 436,688 + Pf6N2E2_369 0.14 +0.9 436,688 + Pf6N2E2_369 0.14 +0.7 436,688 + Pf6N2E2_369 0.14 -0.1 436,688 + Pf6N2E2_369 0.14 +0.6 436,688 + Pf6N2E2_369 0.14 -0.4 436,688 + Pf6N2E2_369 0.14 +0.5 436,689 - Pf6N2E2_369 0.14 -0.9 436,754 + Pf6N2E2_369 0.20 +0.8 436,844 + Pf6N2E2_369 0.28 +0.5 436,844 + Pf6N2E2_369 0.28 +0.9 436,845 - Pf6N2E2_369 0.28 -0.6 436,955 + Pf6N2E2_369 0.39 -0.3 437,405 + Pf6N2E2_369 0.80 -0.3 437,405 + Pf6N2E2_369 0.80 +0.9 437,405 + Pf6N2E2_369 0.80 -0.9 437,405 + Pf6N2E2_369 0.80 -0.2 437,406 - Pf6N2E2_369 0.80 -0.1 437,490 - Pf6N2E2_369 0.88 +0.2
Or see this region's nucleotide sequence