Strain Fitness in Pseudomonas fluorescens FW300-N2E2 around Pf6N2E2_3505

Experiment: 10% P. fluorescens FW300-N2C3 spent media from growth in 20 mM putrescine

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Only strains with sufficient reads to estimate fitness are shown, but the strain fitness values are still rather noisy and may be biased towards zero. Strains near the edge of a gene are not shown as being associated with that gene (they are in grey). Strains in the central 10-90% of a gene are color coded by the insertion's strand. Usually, "+" means that the selectable marker is encoded on the forward strand, i.e., transcribed rightward.

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500 ntPf6N2E2_3504 and Pf6N2E2_3505 are separated by 211 nucleotidesPf6N2E2_3505 and Pf6N2E2_3506 are separated by 168 nucleotidesPf6N2E2_3506 and Pf6N2E2_3507 are separated by 36 nucleotides Pf6N2E2_3504 - Biosynthetic arginine decarboxylase (EC 4.1.1.19), at 4,083,886 to 4,085,799 _3504 Pf6N2E2_3505 - Translation initiation factor SUI1-related protein, at 4,086,011 to 4,086,382 _3505 Pf6N2E2_3506 - Putative Nudix hydrolase YfcD (EC 3.6.-.-), at 4,086,551 to 4,087,075 _3506 Pf6N2E2_3507 - FIG014328: hypothetical protein, at 4,087,112 to 4,088,179 _3507 Position (kb) 4086 4087Strain fitness (log2 ratio) -2 -1 0 1 2at 4085.331 kb on - strand, within Pf6N2E2_3504at 4085.331 kb on - strand, within Pf6N2E2_3504at 4085.492 kb on + strand, within Pf6N2E2_3504at 4085.492 kb on + strand, within Pf6N2E2_3504at 4085.493 kb on - strand, within Pf6N2E2_3504at 4085.493 kb on - strand, within Pf6N2E2_3504at 4085.493 kb on - strand, within Pf6N2E2_3504at 4085.505 kb on - strand, within Pf6N2E2_3504at 4085.811 kb on - strandat 4085.812 kb on + strandat 4085.812 kb on + strandat 4085.813 kb on - strandat 4085.813 kb on - strandat 4085.856 kb on + strandat 4085.857 kb on - strandat 4085.857 kb on - strandat 4085.892 kb on + strandat 4085.892 kb on + strandat 4085.893 kb on - strandat 4085.918 kb on - strandat 4085.918 kb on - strandat 4086.338 kb on + strand, within Pf6N2E2_3505at 4086.338 kb on + strand, within Pf6N2E2_3505at 4086.552 kb on + strandat 4086.552 kb on + strandat 4086.553 kb on - strandat 4086.553 kb on - strandat 4086.554 kb on + strandat 4086.555 kb on - strandat 4086.555 kb on - strandat 4086.591 kb on + strandat 4086.591 kb on + strandat 4086.592 kb on - strandat 4086.624 kb on + strand, within Pf6N2E2_3506at 4086.624 kb on + strand, within Pf6N2E2_3506at 4086.625 kb on - strand, within Pf6N2E2_3506at 4086.768 kb on + strand, within Pf6N2E2_3506at 4086.846 kb on + strand, within Pf6N2E2_3506at 4086.847 kb on - strand, within Pf6N2E2_3506at 4086.900 kb on + strand, within Pf6N2E2_3506at 4086.900 kb on + strand, within Pf6N2E2_3506at 4086.901 kb on - strand, within Pf6N2E2_3506at 4086.966 kb on + strand, within Pf6N2E2_3506at 4086.966 kb on + strand, within Pf6N2E2_3506at 4086.966 kb on + strand, within Pf6N2E2_3506at 4086.966 kb on + strand, within Pf6N2E2_3506at 4086.966 kb on + strand, within Pf6N2E2_3506at 4086.967 kb on - strand, within Pf6N2E2_3506at 4086.967 kb on - strand, within Pf6N2E2_3506at 4086.967 kb on - strand, within Pf6N2E2_3506at 4086.967 kb on - strand, within Pf6N2E2_3506at 4086.967 kb on - strand, within Pf6N2E2_3506at 4086.967 kb on - strand, within Pf6N2E2_3506at 4086.967 kb on - strand, within Pf6N2E2_3506at 4086.967 kb on - strand, within Pf6N2E2_3506at 4086.967 kb on - strand, within Pf6N2E2_3506at 4087.170 kb on + strandat 4087.170 kb on + strandat 4087.202 kb on + strandat 4087.203 kb on - strand

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Per-strain Table

Position Strand Gene LocusTag Fraction 10% P. fluorescens FW300-N2C3 spent media from growth in 20 mM putrescine
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4,085,331 - Pf6N2E2_3504 0.75 +0.4
4,085,331 - Pf6N2E2_3504 0.75 -1.1
4,085,492 + Pf6N2E2_3504 0.84 -2.6
4,085,492 + Pf6N2E2_3504 0.84 +0.2
4,085,493 - Pf6N2E2_3504 0.84 -0.5
4,085,493 - Pf6N2E2_3504 0.84 -0.6
4,085,493 - Pf6N2E2_3504 0.84 -0.4
4,085,505 - Pf6N2E2_3504 0.85 +0.1
4,085,811 - +0.4
4,085,812 + +0.4
4,085,812 + -1.0
4,085,813 - -1.2
4,085,813 - +0.1
4,085,856 + +0.4
4,085,857 - +0.3
4,085,857 - +0.4
4,085,892 + +0.9
4,085,892 + +1.9
4,085,893 - +0.4
4,085,918 - +1.6
4,085,918 - -0.6
4,086,338 + Pf6N2E2_3505 0.88 -1.8
4,086,338 + Pf6N2E2_3505 0.88 +0.4
4,086,552 + +0.7
4,086,552 + -0.8
4,086,553 - -0.2
4,086,553 - -1.6
4,086,554 + +0.2
4,086,555 - +0.4
4,086,555 - -0.5
4,086,591 + +0.4
4,086,591 + +0.4
4,086,592 - +0.6
4,086,624 + Pf6N2E2_3506 0.14 +0.9
4,086,624 + Pf6N2E2_3506 0.14 +0.2
4,086,625 - Pf6N2E2_3506 0.14 +0.4
4,086,768 + Pf6N2E2_3506 0.41 +0.9
4,086,846 + Pf6N2E2_3506 0.56 +1.0
4,086,847 - Pf6N2E2_3506 0.56 -0.8
4,086,900 + Pf6N2E2_3506 0.66 +0.6
4,086,900 + Pf6N2E2_3506 0.66 +1.4
4,086,901 - Pf6N2E2_3506 0.67 -0.4
4,086,966 + Pf6N2E2_3506 0.79 +1.0
4,086,966 + Pf6N2E2_3506 0.79 -1.0
4,086,966 + Pf6N2E2_3506 0.79 -0.2
4,086,966 + Pf6N2E2_3506 0.79 +0.6
4,086,966 + Pf6N2E2_3506 0.79 -0.2
4,086,967 - Pf6N2E2_3506 0.79 -0.5
4,086,967 - Pf6N2E2_3506 0.79 -0.6
4,086,967 - Pf6N2E2_3506 0.79 +0.5
4,086,967 - Pf6N2E2_3506 0.79 -0.4
4,086,967 - Pf6N2E2_3506 0.79 +0.5
4,086,967 - Pf6N2E2_3506 0.79 +0.4
4,086,967 - Pf6N2E2_3506 0.79 -0.3
4,086,967 - Pf6N2E2_3506 0.79 +0.8
4,086,967 - Pf6N2E2_3506 0.79 -1.6
4,087,170 + +0.4
4,087,170 + +1.1
4,087,202 + +0.4
4,087,203 - +0.1

Or see this region's nucleotide sequence