Experiment: 10% P. fluorescens FW300-N2C3 spent media from growth in 20 mM putrescine
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt Pf6N2E2_3504 and Pf6N2E2_3505 are separated by 211 nucleotides Pf6N2E2_3505 and Pf6N2E2_3506 are separated by 168 nucleotides Pf6N2E2_3506 and Pf6N2E2_3507 are separated by 36 nucleotides
Pf6N2E2_3504 - Biosynthetic arginine decarboxylase (EC 4.1.1.19), at 4,083,886 to 4,085,799
_3504
Pf6N2E2_3505 - Translation initiation factor SUI1-related protein, at 4,086,011 to 4,086,382
_3505
Pf6N2E2_3506 - Putative Nudix hydrolase YfcD (EC 3.6.-.-), at 4,086,551 to 4,087,075
_3506
Pf6N2E2_3507 - FIG014328: hypothetical protein, at 4,087,112 to 4,088,179
_3507
Position (kb)
4086
4087 Strain fitness (log2 ratio)
-2
-1
0
1
2 at 4085.331 kb on - strand, within Pf6N2E2_3504 at 4085.331 kb on - strand, within Pf6N2E2_3504 at 4085.492 kb on + strand, within Pf6N2E2_3504 at 4085.492 kb on + strand, within Pf6N2E2_3504 at 4085.493 kb on - strand, within Pf6N2E2_3504 at 4085.493 kb on - strand, within Pf6N2E2_3504 at 4085.493 kb on - strand, within Pf6N2E2_3504 at 4085.505 kb on - strand, within Pf6N2E2_3504 at 4085.811 kb on - strand at 4085.812 kb on + strand at 4085.812 kb on + strand at 4085.813 kb on - strand at 4085.813 kb on - strand at 4085.856 kb on + strand at 4085.857 kb on - strand at 4085.857 kb on - strand at 4085.892 kb on + strand at 4085.892 kb on + strand at 4085.893 kb on - strand at 4085.918 kb on - strand at 4085.918 kb on - strand at 4086.338 kb on + strand, within Pf6N2E2_3505 at 4086.338 kb on + strand, within Pf6N2E2_3505 at 4086.552 kb on + strand at 4086.552 kb on + strand at 4086.553 kb on - strand at 4086.553 kb on - strand at 4086.554 kb on + strand at 4086.555 kb on - strand at 4086.555 kb on - strand at 4086.591 kb on + strand at 4086.591 kb on + strand at 4086.592 kb on - strand at 4086.624 kb on + strand, within Pf6N2E2_3506 at 4086.624 kb on + strand, within Pf6N2E2_3506 at 4086.625 kb on - strand, within Pf6N2E2_3506 at 4086.768 kb on + strand, within Pf6N2E2_3506 at 4086.846 kb on + strand, within Pf6N2E2_3506 at 4086.847 kb on - strand, within Pf6N2E2_3506 at 4086.900 kb on + strand, within Pf6N2E2_3506 at 4086.900 kb on + strand, within Pf6N2E2_3506 at 4086.901 kb on - strand, within Pf6N2E2_3506 at 4086.966 kb on + strand, within Pf6N2E2_3506 at 4086.966 kb on + strand, within Pf6N2E2_3506 at 4086.966 kb on + strand, within Pf6N2E2_3506 at 4086.966 kb on + strand, within Pf6N2E2_3506 at 4086.966 kb on + strand, within Pf6N2E2_3506 at 4086.967 kb on - strand, within Pf6N2E2_3506 at 4086.967 kb on - strand, within Pf6N2E2_3506 at 4086.967 kb on - strand, within Pf6N2E2_3506 at 4086.967 kb on - strand, within Pf6N2E2_3506 at 4086.967 kb on - strand, within Pf6N2E2_3506 at 4086.967 kb on - strand, within Pf6N2E2_3506 at 4086.967 kb on - strand, within Pf6N2E2_3506 at 4086.967 kb on - strand, within Pf6N2E2_3506 at 4086.967 kb on - strand, within Pf6N2E2_3506 at 4087.170 kb on + strand at 4087.170 kb on + strand at 4087.202 kb on + strand at 4087.203 kb on - strand
Per-strain Table
Position Strand Gene LocusTag Fraction 10% P. fluorescens FW300-N2C3 spent media from growth in 20 mM putrescine remove 4,085,331 - Pf6N2E2_3504 0.75 +0.4 4,085,331 - Pf6N2E2_3504 0.75 -1.1 4,085,492 + Pf6N2E2_3504 0.84 -2.6 4,085,492 + Pf6N2E2_3504 0.84 +0.2 4,085,493 - Pf6N2E2_3504 0.84 -0.5 4,085,493 - Pf6N2E2_3504 0.84 -0.6 4,085,493 - Pf6N2E2_3504 0.84 -0.4 4,085,505 - Pf6N2E2_3504 0.85 +0.1 4,085,811 - +0.4 4,085,812 + +0.4 4,085,812 + -1.0 4,085,813 - -1.2 4,085,813 - +0.1 4,085,856 + +0.4 4,085,857 - +0.3 4,085,857 - +0.4 4,085,892 + +0.9 4,085,892 + +1.9 4,085,893 - +0.4 4,085,918 - +1.6 4,085,918 - -0.6 4,086,338 + Pf6N2E2_3505 0.88 -1.8 4,086,338 + Pf6N2E2_3505 0.88 +0.4 4,086,552 + +0.7 4,086,552 + -0.8 4,086,553 - -0.2 4,086,553 - -1.6 4,086,554 + +0.2 4,086,555 - +0.4 4,086,555 - -0.5 4,086,591 + +0.4 4,086,591 + +0.4 4,086,592 - +0.6 4,086,624 + Pf6N2E2_3506 0.14 +0.9 4,086,624 + Pf6N2E2_3506 0.14 +0.2 4,086,625 - Pf6N2E2_3506 0.14 +0.4 4,086,768 + Pf6N2E2_3506 0.41 +0.9 4,086,846 + Pf6N2E2_3506 0.56 +1.0 4,086,847 - Pf6N2E2_3506 0.56 -0.8 4,086,900 + Pf6N2E2_3506 0.66 +0.6 4,086,900 + Pf6N2E2_3506 0.66 +1.4 4,086,901 - Pf6N2E2_3506 0.67 -0.4 4,086,966 + Pf6N2E2_3506 0.79 +1.0 4,086,966 + Pf6N2E2_3506 0.79 -1.0 4,086,966 + Pf6N2E2_3506 0.79 -0.2 4,086,966 + Pf6N2E2_3506 0.79 +0.6 4,086,966 + Pf6N2E2_3506 0.79 -0.2 4,086,967 - Pf6N2E2_3506 0.79 -0.5 4,086,967 - Pf6N2E2_3506 0.79 -0.6 4,086,967 - Pf6N2E2_3506 0.79 +0.5 4,086,967 - Pf6N2E2_3506 0.79 -0.4 4,086,967 - Pf6N2E2_3506 0.79 +0.5 4,086,967 - Pf6N2E2_3506 0.79 +0.4 4,086,967 - Pf6N2E2_3506 0.79 -0.3 4,086,967 - Pf6N2E2_3506 0.79 +0.8 4,086,967 - Pf6N2E2_3506 0.79 -1.6 4,087,170 + +0.4 4,087,170 + +1.1 4,087,202 + +0.4 4,087,203 - +0.1
Or see this region's nucleotide sequence