Experiment: 10% P. fluorescens FW300-N2C3 spent media from growth in 20 mM putrescine
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt Pf6N2E2_3121 and Pf6N2E2_3122 overlap by 4 nucleotides Pf6N2E2_3122 and Pf6N2E2_3123 overlap by 1 nucleotides
Pf6N2E2_3121 - Chemotaxis protein methyltransferase CheR (EC 2.1.1.80), at 3,611,012 to 3,612,277
_3121
Pf6N2E2_3122 - Chemotaxis signal transduction protein, at 3,612,274 to 3,612,810
_3122
Pf6N2E2_3123 - Methyl-accepting chemotaxis protein I (serine chemoreceptor protein), at 3,612,810 to 3,614,432
_3123
Position (kb)
3612
3613 Strain fitness (log2 ratio)
-2
-1
0
1
2 at 3611.317 kb on + strand, within Pf6N2E2_3121 at 3611.570 kb on - strand, within Pf6N2E2_3121 at 3611.787 kb on + strand, within Pf6N2E2_3121 at 3611.787 kb on + strand, within Pf6N2E2_3121 at 3611.787 kb on + strand, within Pf6N2E2_3121 at 3611.944 kb on - strand, within Pf6N2E2_3121 at 3612.141 kb on + strand, within Pf6N2E2_3121 at 3612.141 kb on + strand, within Pf6N2E2_3121 at 3612.142 kb on - strand, within Pf6N2E2_3121 at 3612.411 kb on - strand, within Pf6N2E2_3122 at 3612.411 kb on - strand, within Pf6N2E2_3122 at 3612.438 kb on - strand, within Pf6N2E2_3122 at 3613.162 kb on + strand, within Pf6N2E2_3123 at 3613.162 kb on + strand, within Pf6N2E2_3123 at 3613.162 kb on + strand, within Pf6N2E2_3123 at 3613.162 kb on + strand, within Pf6N2E2_3123 at 3613.163 kb on - strand, within Pf6N2E2_3123 at 3613.163 kb on - strand, within Pf6N2E2_3123 at 3613.163 kb on - strand, within Pf6N2E2_3123 at 3613.163 kb on - strand, within Pf6N2E2_3123 at 3613.163 kb on - strand, within Pf6N2E2_3123 at 3613.197 kb on + strand, within Pf6N2E2_3123 at 3613.197 kb on + strand, within Pf6N2E2_3123 at 3613.198 kb on - strand, within Pf6N2E2_3123 at 3613.198 kb on - strand, within Pf6N2E2_3123 at 3613.198 kb on - strand, within Pf6N2E2_3123 at 3613.232 kb on - strand, within Pf6N2E2_3123 at 3613.810 kb on - strand, within Pf6N2E2_3123
Per-strain Table
Position Strand Gene LocusTag Fraction 10% P. fluorescens FW300-N2C3 spent media from growth in 20 mM putrescine remove 3,611,317 + Pf6N2E2_3121 0.24 +1.2 3,611,570 - Pf6N2E2_3121 0.44 +0.9 3,611,787 + Pf6N2E2_3121 0.61 -0.0 3,611,787 + Pf6N2E2_3121 0.61 +1.2 3,611,787 + Pf6N2E2_3121 0.61 -0.1 3,611,944 - Pf6N2E2_3121 0.74 -0.1 3,612,141 + Pf6N2E2_3121 0.89 -0.8 3,612,141 + Pf6N2E2_3121 0.89 -0.8 3,612,142 - Pf6N2E2_3121 0.89 +0.2 3,612,411 - Pf6N2E2_3122 0.26 -0.6 3,612,411 - Pf6N2E2_3122 0.26 +0.2 3,612,438 - Pf6N2E2_3122 0.31 -1.8 3,613,162 + Pf6N2E2_3123 0.22 -0.8 3,613,162 + Pf6N2E2_3123 0.22 +0.4 3,613,162 + Pf6N2E2_3123 0.22 +1.0 3,613,162 + Pf6N2E2_3123 0.22 +0.1 3,613,163 - Pf6N2E2_3123 0.22 +1.2 3,613,163 - Pf6N2E2_3123 0.22 -0.3 3,613,163 - Pf6N2E2_3123 0.22 +0.2 3,613,163 - Pf6N2E2_3123 0.22 +0.2 3,613,163 - Pf6N2E2_3123 0.22 -0.4 3,613,197 + Pf6N2E2_3123 0.24 -0.3 3,613,197 + Pf6N2E2_3123 0.24 -0.0 3,613,198 - Pf6N2E2_3123 0.24 +0.6 3,613,198 - Pf6N2E2_3123 0.24 +0.8 3,613,198 - Pf6N2E2_3123 0.24 +0.0 3,613,232 - Pf6N2E2_3123 0.26 +1.8 3,613,810 - Pf6N2E2_3123 0.62 +0.2
Or see this region's nucleotide sequence