Experiment: 10% P. fluorescens FW300-N2C3 spent media from growth in 20 mM putrescine
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt Pf6N2E2_2585 and Pf6N2E2_2586 are separated by 88 nucleotides Pf6N2E2_2586 and Pf6N2E2_2587 are separated by 31 nucleotides Pf6N2E2_2587 and Pf6N2E2_2588 are separated by 5 nucleotides Pf6N2E2_2588 and Pf6N2E2_2589 are separated by 141 nucleotides Pf6N2E2_2589 and Pf6N2E2_2590 are separated by 6 nucleotides
Pf6N2E2_2585 - Flagellar hook-length control protein FliK, at 3,042,373 to 3,043,746
_2585
Pf6N2E2_2586 - Hpt domain protein, at 3,043,835 to 3,044,179
_2586
Pf6N2E2_2587 - Serine phosphatase RsbU, regulator of sigma subunit, at 3,044,211 to 3,045,917
_2587
Pf6N2E2_2588 - Anti-sigma F factor antagonist (spoIIAA-2); Anti-sigma B factor antagonist RsbV, at 3,045,923 to 3,046,189
_2588
Pf6N2E2_2589 - Flagellar protein FliJ, at 3,046,331 to 3,046,780
_2589
Pf6N2E2_2590 - Flagellum-specific ATP synthase FliI, at 3,046,787 to 3,048,145
_2590
Position (kb)
3044
3045
3046 Strain fitness (log2 ratio)
-3
-2
-1
0
1
2 at 3043.549 kb on - strand, within Pf6N2E2_2585 at 3043.650 kb on + strand at 3043.728 kb on + strand at 3043.728 kb on + strand at 3043.729 kb on - strand at 3043.729 kb on - strand at 3043.729 kb on - strand at 3043.774 kb on + strand at 3043.775 kb on - strand at 3043.775 kb on - strand at 3043.775 kb on - strand at 3043.775 kb on - strand at 3043.779 kb on - strand at 3043.779 kb on - strand at 3043.881 kb on + strand, within Pf6N2E2_2586 at 3043.882 kb on - strand, within Pf6N2E2_2586 at 3044.018 kb on - strand, within Pf6N2E2_2586 at 3044.388 kb on + strand, within Pf6N2E2_2587 at 3044.388 kb on + strand, within Pf6N2E2_2587 at 3044.388 kb on + strand, within Pf6N2E2_2587 at 3044.443 kb on + strand, within Pf6N2E2_2587 at 3044.443 kb on + strand, within Pf6N2E2_2587 at 3044.444 kb on - strand, within Pf6N2E2_2587 at 3044.444 kb on - strand, within Pf6N2E2_2587 at 3044.485 kb on + strand, within Pf6N2E2_2587 at 3044.485 kb on + strand, within Pf6N2E2_2587 at 3044.485 kb on + strand, within Pf6N2E2_2587 at 3044.486 kb on - strand, within Pf6N2E2_2587 at 3044.486 kb on - strand, within Pf6N2E2_2587 at 3044.489 kb on - strand, within Pf6N2E2_2587 at 3044.489 kb on - strand, within Pf6N2E2_2587 at 3044.563 kb on + strand, within Pf6N2E2_2587 at 3044.564 kb on - strand, within Pf6N2E2_2587 at 3044.564 kb on - strand, within Pf6N2E2_2587 at 3044.564 kb on - strand, within Pf6N2E2_2587 at 3044.584 kb on + strand, within Pf6N2E2_2587 at 3044.585 kb on - strand, within Pf6N2E2_2587 at 3044.585 kb on - strand, within Pf6N2E2_2587 at 3044.625 kb on + strand, within Pf6N2E2_2587 at 3044.626 kb on - strand, within Pf6N2E2_2587 at 3044.626 kb on - strand, within Pf6N2E2_2587 at 3044.626 kb on - strand, within Pf6N2E2_2587 at 3044.641 kb on + strand, within Pf6N2E2_2587 at 3044.641 kb on + strand, within Pf6N2E2_2587 at 3044.641 kb on + strand, within Pf6N2E2_2587 at 3044.641 kb on + strand, within Pf6N2E2_2587 at 3044.641 kb on + strand, within Pf6N2E2_2587 at 3044.642 kb on - strand, within Pf6N2E2_2587 at 3044.978 kb on + strand, within Pf6N2E2_2587 at 3044.978 kb on + strand, within Pf6N2E2_2587 at 3044.978 kb on + strand, within Pf6N2E2_2587 at 3044.978 kb on + strand, within Pf6N2E2_2587 at 3044.979 kb on - strand, within Pf6N2E2_2587 at 3044.979 kb on - strand, within Pf6N2E2_2587 at 3044.979 kb on - strand, within Pf6N2E2_2587 at 3044.979 kb on - strand, within Pf6N2E2_2587 at 3044.979 kb on - strand, within Pf6N2E2_2587 at 3044.979 kb on - strand, within Pf6N2E2_2587 at 3044.979 kb on - strand, within Pf6N2E2_2587 at 3045.053 kb on + strand, within Pf6N2E2_2587 at 3045.083 kb on - strand, within Pf6N2E2_2587 at 3045.141 kb on + strand, within Pf6N2E2_2587 at 3045.141 kb on + strand, within Pf6N2E2_2587 at 3045.141 kb on + strand, within Pf6N2E2_2587 at 3045.141 kb on + strand, within Pf6N2E2_2587 at 3045.142 kb on - strand, within Pf6N2E2_2587 at 3045.142 kb on - strand, within Pf6N2E2_2587 at 3045.142 kb on - strand, within Pf6N2E2_2587 at 3045.379 kb on + strand, within Pf6N2E2_2587 at 3045.379 kb on + strand, within Pf6N2E2_2587 at 3045.380 kb on - strand, within Pf6N2E2_2587 at 3045.394 kb on + strand, within Pf6N2E2_2587 at 3045.394 kb on + strand, within Pf6N2E2_2587 at 3045.394 kb on + strand, within Pf6N2E2_2587 at 3045.395 kb on - strand, within Pf6N2E2_2587 at 3045.478 kb on + strand, within Pf6N2E2_2587 at 3045.478 kb on + strand, within Pf6N2E2_2587 at 3045.478 kb on + strand, within Pf6N2E2_2587 at 3045.478 kb on + strand, within Pf6N2E2_2587 at 3045.479 kb on - strand, within Pf6N2E2_2587 at 3045.479 kb on - strand, within Pf6N2E2_2587 at 3045.479 kb on - strand, within Pf6N2E2_2587 at 3045.479 kb on - strand, within Pf6N2E2_2587 at 3045.479 kb on - strand, within Pf6N2E2_2587 at 3045.583 kb on + strand, within Pf6N2E2_2587 at 3045.779 kb on - strand at 3046.034 kb on + strand, within Pf6N2E2_2588 at 3046.035 kb on - strand, within Pf6N2E2_2588 at 3046.035 kb on - strand, within Pf6N2E2_2588 at 3046.071 kb on + strand, within Pf6N2E2_2588 at 3046.072 kb on - strand, within Pf6N2E2_2588 at 3046.072 kb on - strand, within Pf6N2E2_2588 at 3046.252 kb on + strand at 3046.252 kb on + strand at 3046.253 kb on - strand at 3046.253 kb on - strand at 3046.253 kb on - strand at 3046.253 kb on - strand at 3046.253 kb on - strand at 3046.253 kb on - strand at 3046.258 kb on + strand at 3046.258 kb on + strand at 3046.258 kb on + strand at 3046.259 kb on - strand at 3046.259 kb on - strand at 3046.259 kb on - strand at 3046.259 kb on - strand at 3046.259 kb on - strand at 3046.259 kb on - strand at 3046.259 kb on - strand at 3046.267 kb on + strand at 3046.268 kb on - strand at 3046.278 kb on - strand at 3046.278 kb on - strand at 3046.420 kb on + strand, within Pf6N2E2_2589 at 3046.421 kb on - strand, within Pf6N2E2_2589 at 3046.422 kb on + strand, within Pf6N2E2_2589 at 3046.422 kb on + strand, within Pf6N2E2_2589 at 3046.422 kb on + strand, within Pf6N2E2_2589 at 3046.423 kb on - strand, within Pf6N2E2_2589 at 3046.423 kb on - strand, within Pf6N2E2_2589 at 3046.423 kb on - strand, within Pf6N2E2_2589 at 3046.630 kb on - strand, within Pf6N2E2_2589 at 3046.789 kb on - strand at 3046.789 kb on - strand at 3046.813 kb on + strand at 3046.814 kb on - strand
Per-strain Table
Position Strand Gene LocusTag Fraction 10% P. fluorescens FW300-N2C3 spent media from growth in 20 mM putrescine remove 3,043,549 - Pf6N2E2_2585 0.86 +0.7 3,043,650 + +1.4 3,043,728 + +1.4 3,043,728 + -0.1 3,043,729 - +0.7 3,043,729 - +1.2 3,043,729 - +0.1 3,043,774 + +1.2 3,043,775 - -0.5 3,043,775 - -0.1 3,043,775 - -0.1 3,043,775 - +0.7 3,043,779 - -2.4 3,043,779 - -1.7 3,043,881 + Pf6N2E2_2586 0.13 -0.1 3,043,882 - Pf6N2E2_2586 0.14 -1.1 3,044,018 - Pf6N2E2_2586 0.53 -0.1 3,044,388 + Pf6N2E2_2587 0.10 -2.1 3,044,388 + Pf6N2E2_2587 0.10 -2.2 3,044,388 + Pf6N2E2_2587 0.10 -1.4 3,044,443 + Pf6N2E2_2587 0.14 -0.1 3,044,443 + Pf6N2E2_2587 0.14 -1.1 3,044,444 - Pf6N2E2_2587 0.14 +1.8 3,044,444 - Pf6N2E2_2587 0.14 -1.5 3,044,485 + Pf6N2E2_2587 0.16 -0.4 3,044,485 + Pf6N2E2_2587 0.16 -2.4 3,044,485 + Pf6N2E2_2587 0.16 -0.9 3,044,486 - Pf6N2E2_2587 0.16 +0.9 3,044,486 - Pf6N2E2_2587 0.16 +1.7 3,044,489 - Pf6N2E2_2587 0.16 +0.5 3,044,489 - Pf6N2E2_2587 0.16 +0.7 3,044,563 + Pf6N2E2_2587 0.21 -0.4 3,044,564 - Pf6N2E2_2587 0.21 -0.9 3,044,564 - Pf6N2E2_2587 0.21 -1.7 3,044,564 - Pf6N2E2_2587 0.21 +0.9 3,044,584 + Pf6N2E2_2587 0.22 -0.1 3,044,585 - Pf6N2E2_2587 0.22 -0.5 3,044,585 - Pf6N2E2_2587 0.22 -2.2 3,044,625 + Pf6N2E2_2587 0.24 -1.4 3,044,626 - Pf6N2E2_2587 0.24 +0.5 3,044,626 - Pf6N2E2_2587 0.24 -0.1 3,044,626 - Pf6N2E2_2587 0.24 -0.1 3,044,641 + Pf6N2E2_2587 0.25 -1.5 3,044,641 + Pf6N2E2_2587 0.25 -1.5 3,044,641 + Pf6N2E2_2587 0.25 -1.2 3,044,641 + Pf6N2E2_2587 0.25 -2.9 3,044,641 + Pf6N2E2_2587 0.25 -0.3 3,044,642 - Pf6N2E2_2587 0.25 +0.3 3,044,978 + Pf6N2E2_2587 0.45 +0.4 3,044,978 + Pf6N2E2_2587 0.45 -0.8 3,044,978 + Pf6N2E2_2587 0.45 -1.1 3,044,978 + Pf6N2E2_2587 0.45 -0.8 3,044,979 - Pf6N2E2_2587 0.45 -1.5 3,044,979 - Pf6N2E2_2587 0.45 -0.9 3,044,979 - Pf6N2E2_2587 0.45 +0.1 3,044,979 - Pf6N2E2_2587 0.45 +2.5 3,044,979 - Pf6N2E2_2587 0.45 -0.1 3,044,979 - Pf6N2E2_2587 0.45 -0.3 3,044,979 - Pf6N2E2_2587 0.45 -1.6 3,045,053 + Pf6N2E2_2587 0.49 -0.8 3,045,083 - Pf6N2E2_2587 0.51 -0.1 3,045,141 + Pf6N2E2_2587 0.54 -0.5 3,045,141 + Pf6N2E2_2587 0.54 -0.1 3,045,141 + Pf6N2E2_2587 0.54 -0.3 3,045,141 + Pf6N2E2_2587 0.54 -0.1 3,045,142 - Pf6N2E2_2587 0.55 -0.2 3,045,142 - Pf6N2E2_2587 0.55 -1.0 3,045,142 - Pf6N2E2_2587 0.55 -0.4 3,045,379 + Pf6N2E2_2587 0.68 -1.7 3,045,379 + Pf6N2E2_2587 0.68 +0.9 3,045,380 - Pf6N2E2_2587 0.68 -0.8 3,045,394 + Pf6N2E2_2587 0.69 -1.0 3,045,394 + Pf6N2E2_2587 0.69 -1.4 3,045,394 + Pf6N2E2_2587 0.69 -0.7 3,045,395 - Pf6N2E2_2587 0.69 -0.1 3,045,478 + Pf6N2E2_2587 0.74 -0.1 3,045,478 + Pf6N2E2_2587 0.74 -0.1 3,045,478 + Pf6N2E2_2587 0.74 -1.1 3,045,478 + Pf6N2E2_2587 0.74 -1.7 3,045,479 - Pf6N2E2_2587 0.74 -0.6 3,045,479 - Pf6N2E2_2587 0.74 -0.7 3,045,479 - Pf6N2E2_2587 0.74 -1.1 3,045,479 - Pf6N2E2_2587 0.74 -0.7 3,045,479 - Pf6N2E2_2587 0.74 -0.1 3,045,583 + Pf6N2E2_2587 0.80 -0.8 3,045,779 - -0.1 3,046,034 + Pf6N2E2_2588 0.42 -1.3 3,046,035 - Pf6N2E2_2588 0.42 -2.2 3,046,035 - Pf6N2E2_2588 0.42 -1.7 3,046,071 + Pf6N2E2_2588 0.55 -1.1 3,046,072 - Pf6N2E2_2588 0.56 -0.8 3,046,072 - Pf6N2E2_2588 0.56 +0.9 3,046,252 + -0.1 3,046,252 + -2.1 3,046,253 - -0.1 3,046,253 - -0.7 3,046,253 - -0.1 3,046,253 - -0.4 3,046,253 - -0.1 3,046,253 - -1.1 3,046,258 + -2.1 3,046,258 + -0.1 3,046,258 + -0.7 3,046,259 - -2.1 3,046,259 - +1.2 3,046,259 - -0.8 3,046,259 - -1.1 3,046,259 - -0.1 3,046,259 - -0.7 3,046,259 - -2.9 3,046,267 + -1.3 3,046,268 - -0.1 3,046,278 - +0.4 3,046,278 - +0.5 3,046,420 + Pf6N2E2_2589 0.20 +0.6 3,046,421 - Pf6N2E2_2589 0.20 +1.0 3,046,422 + Pf6N2E2_2589 0.20 +1.1 3,046,422 + Pf6N2E2_2589 0.20 +0.5 3,046,422 + Pf6N2E2_2589 0.20 -0.1 3,046,423 - Pf6N2E2_2589 0.20 +1.0 3,046,423 - Pf6N2E2_2589 0.20 +1.4 3,046,423 - Pf6N2E2_2589 0.20 +1.8 3,046,630 - Pf6N2E2_2589 0.66 -0.1 3,046,789 - +1.1 3,046,789 - +0.3 3,046,813 + +0.9 3,046,814 - +1.0
Or see this region's nucleotide sequence