Strain Fitness in Pseudomonas fluorescens FW300-N2E2 around Pf6N2E2_2406

Experiment: 10% P. fluorescens FW300-N2C3 spent media from growth in 20 mM putrescine

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Only strains with sufficient reads to estimate fitness are shown, but the strain fitness values are still rather noisy and may be biased towards zero. Strains near the edge of a gene are not shown as being associated with that gene (they are in grey). Strains in the central 10-90% of a gene are color coded by the insertion's strand. Usually, "+" means that the selectable marker is encoded on the forward strand, i.e., transcribed rightward.

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500 ntPf6N2E2_2405 and Pf6N2E2_2406 are separated by 70 nucleotidesPf6N2E2_2406 and Pf6N2E2_2407 are separated by 97 nucleotidesPf6N2E2_2407 and Pf6N2E2_2408 are separated by 180 nucleotides Pf6N2E2_2405 - Hypothetical protein PvdY @ Siderophore synthetase small component, acetyltransferase, at 2,855,817 to 2,856,830 _2405 Pf6N2E2_2406 - Periplasmic binding protein, at 2,856,901 to 2,857,698 _2406 Pf6N2E2_2407 - Acyclic terpenes utilization regulator AtuR, TetR family, at 2,857,796 to 2,858,422 _2407 Pf6N2E2_2408 - 3-hydroxy-3isohexenylglutaryl-CoA:acetate lyase, at 2,858,603 to 2,860,408 _2408 Position (kb) 2856 2857 2858Strain fitness (log2 ratio) -2 -1 0 1 2at 2856.004 kb on + strand, within Pf6N2E2_2405at 2856.004 kb on + strand, within Pf6N2E2_2405at 2856.005 kb on - strand, within Pf6N2E2_2405at 2856.091 kb on + strand, within Pf6N2E2_2405at 2856.091 kb on + strand, within Pf6N2E2_2405at 2856.092 kb on - strand, within Pf6N2E2_2405at 2856.092 kb on - strand, within Pf6N2E2_2405at 2856.107 kb on - strand, within Pf6N2E2_2405at 2856.139 kb on + strand, within Pf6N2E2_2405at 2856.140 kb on - strand, within Pf6N2E2_2405at 2856.140 kb on - strand, within Pf6N2E2_2405at 2856.151 kb on + strand, within Pf6N2E2_2405at 2856.152 kb on - strand, within Pf6N2E2_2405at 2856.223 kb on + strand, within Pf6N2E2_2405at 2856.224 kb on - strand, within Pf6N2E2_2405at 2856.483 kb on + strand, within Pf6N2E2_2405at 2856.484 kb on - strand, within Pf6N2E2_2405at 2856.629 kb on - strand, within Pf6N2E2_2405at 2856.845 kb on + strandat 2857.046 kb on + strand, within Pf6N2E2_2406at 2857.047 kb on - strand, within Pf6N2E2_2406at 2857.109 kb on + strand, within Pf6N2E2_2406at 2857.109 kb on + strand, within Pf6N2E2_2406at 2857.109 kb on + strand, within Pf6N2E2_2406at 2857.109 kb on + strand, within Pf6N2E2_2406at 2857.110 kb on - strand, within Pf6N2E2_2406at 2857.110 kb on - strand, within Pf6N2E2_2406at 2857.110 kb on - strand, within Pf6N2E2_2406at 2857.110 kb on - strand, within Pf6N2E2_2406at 2857.122 kb on - strand, within Pf6N2E2_2406at 2857.190 kb on + strand, within Pf6N2E2_2406at 2857.190 kb on + strand, within Pf6N2E2_2406at 2857.190 kb on + strand, within Pf6N2E2_2406at 2857.191 kb on - strand, within Pf6N2E2_2406at 2857.191 kb on - strand, within Pf6N2E2_2406at 2857.191 kb on - strand, within Pf6N2E2_2406at 2857.208 kb on + strand, within Pf6N2E2_2406at 2857.208 kb on + strand, within Pf6N2E2_2406at 2857.208 kb on + strand, within Pf6N2E2_2406at 2857.209 kb on - strand, within Pf6N2E2_2406at 2857.209 kb on - strand, within Pf6N2E2_2406at 2857.209 kb on - strand, within Pf6N2E2_2406at 2857.209 kb on - strand, within Pf6N2E2_2406at 2857.257 kb on - strand, within Pf6N2E2_2406at 2857.257 kb on - strand, within Pf6N2E2_2406at 2857.576 kb on - strand, within Pf6N2E2_2406at 2857.576 kb on - strand, within Pf6N2E2_2406at 2857.577 kb on + strand, within Pf6N2E2_2406at 2857.578 kb on - strand, within Pf6N2E2_2406at 2857.578 kb on - strand, within Pf6N2E2_2406at 2857.578 kb on - strand, within Pf6N2E2_2406at 2857.578 kb on - strand, within Pf6N2E2_2406at 2857.578 kb on - strand, within Pf6N2E2_2406at 2857.729 kb on + strandat 2857.730 kb on - strandat 2857.742 kb on - strandat 2857.759 kb on + strandat 2857.760 kb on - strandat 2857.781 kb on + strandat 2857.782 kb on - strandat 2857.782 kb on - strandat 2857.782 kb on - strandat 2857.782 kb on - strandat 2857.786 kb on + strandat 2857.786 kb on + strandat 2857.786 kb on + strandat 2857.786 kb on + strandat 2857.787 kb on - strandat 2857.797 kb on + strandat 2857.939 kb on + strand, within Pf6N2E2_2407at 2857.939 kb on + strand, within Pf6N2E2_2407at 2857.940 kb on - strand, within Pf6N2E2_2407at 2857.940 kb on - strand, within Pf6N2E2_2407at 2857.940 kb on - strand, within Pf6N2E2_2407at 2857.942 kb on - strand, within Pf6N2E2_2407at 2857.942 kb on - strand, within Pf6N2E2_2407at 2857.989 kb on + strand, within Pf6N2E2_2407at 2857.990 kb on - strand, within Pf6N2E2_2407at 2857.990 kb on - strand, within Pf6N2E2_2407at 2857.990 kb on - strand, within Pf6N2E2_2407at 2857.994 kb on - strand, within Pf6N2E2_2407at 2857.994 kb on - strand, within Pf6N2E2_2407at 2858.049 kb on + strand, within Pf6N2E2_2407at 2858.050 kb on - strand, within Pf6N2E2_2407at 2858.050 kb on - strand, within Pf6N2E2_2407at 2858.435 kb on - strandat 2858.495 kb on + strandat 2858.656 kb on + strandat 2858.656 kb on + strandat 2858.657 kb on - strandat 2858.696 kb on + strandat 2858.696 kb on + strandat 2858.697 kb on - strandat 2858.697 kb on - strand

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Per-strain Table

Position Strand Gene LocusTag Fraction 10% P. fluorescens FW300-N2C3 spent media from growth in 20 mM putrescine
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2,856,004 + Pf6N2E2_2405 0.18 +0.4
2,856,004 + Pf6N2E2_2405 0.18 -0.1
2,856,005 - Pf6N2E2_2405 0.19 -0.5
2,856,091 + Pf6N2E2_2405 0.27 -1.1
2,856,091 + Pf6N2E2_2405 0.27 -0.8
2,856,092 - Pf6N2E2_2405 0.27 -0.1
2,856,092 - Pf6N2E2_2405 0.27 +0.3
2,856,107 - Pf6N2E2_2405 0.29 +0.5
2,856,139 + Pf6N2E2_2405 0.32 -1.3
2,856,140 - Pf6N2E2_2405 0.32 +0.1
2,856,140 - Pf6N2E2_2405 0.32 +0.3
2,856,151 + Pf6N2E2_2405 0.33 -0.4
2,856,152 - Pf6N2E2_2405 0.33 -0.1
2,856,223 + Pf6N2E2_2405 0.40 +0.4
2,856,224 - Pf6N2E2_2405 0.40 +1.9
2,856,483 + Pf6N2E2_2405 0.66 -1.1
2,856,484 - Pf6N2E2_2405 0.66 -0.4
2,856,629 - Pf6N2E2_2405 0.80 +0.5
2,856,845 + -1.3
2,857,046 + Pf6N2E2_2406 0.18 +0.3
2,857,047 - Pf6N2E2_2406 0.18 +0.9
2,857,109 + Pf6N2E2_2406 0.26 +0.9
2,857,109 + Pf6N2E2_2406 0.26 -0.1
2,857,109 + Pf6N2E2_2406 0.26 +1.7
2,857,109 + Pf6N2E2_2406 0.26 +0.9
2,857,110 - Pf6N2E2_2406 0.26 +0.6
2,857,110 - Pf6N2E2_2406 0.26 +0.5
2,857,110 - Pf6N2E2_2406 0.26 -0.1
2,857,110 - Pf6N2E2_2406 0.26 -0.1
2,857,122 - Pf6N2E2_2406 0.28 +0.9
2,857,190 + Pf6N2E2_2406 0.36 +0.9
2,857,190 + Pf6N2E2_2406 0.36 +1.2
2,857,190 + Pf6N2E2_2406 0.36 -0.1
2,857,191 - Pf6N2E2_2406 0.36 -0.1
2,857,191 - Pf6N2E2_2406 0.36 +1.6
2,857,191 - Pf6N2E2_2406 0.36 -0.1
2,857,208 + Pf6N2E2_2406 0.38 +1.5
2,857,208 + Pf6N2E2_2406 0.38 +1.4
2,857,208 + Pf6N2E2_2406 0.38 +0.7
2,857,209 - Pf6N2E2_2406 0.39 +1.5
2,857,209 - Pf6N2E2_2406 0.39 +1.1
2,857,209 - Pf6N2E2_2406 0.39 +0.6
2,857,209 - Pf6N2E2_2406 0.39 -0.1
2,857,257 - Pf6N2E2_2406 0.45 +0.5
2,857,257 - Pf6N2E2_2406 0.45 +0.6
2,857,576 - Pf6N2E2_2406 0.85 +0.7
2,857,576 - Pf6N2E2_2406 0.85 -1.1
2,857,577 + Pf6N2E2_2406 0.85 +0.0
2,857,578 - Pf6N2E2_2406 0.85 -0.0
2,857,578 - Pf6N2E2_2406 0.85 -0.1
2,857,578 - Pf6N2E2_2406 0.85 -0.1
2,857,578 - Pf6N2E2_2406 0.85 +0.9
2,857,578 - Pf6N2E2_2406 0.85 +0.7
2,857,729 + +1.0
2,857,730 - -0.1
2,857,742 - +0.4
2,857,759 + -0.2
2,857,760 - -1.1
2,857,781 + -0.1
2,857,782 - -0.4
2,857,782 - -0.1
2,857,782 - -2.1
2,857,782 - -0.5
2,857,786 + +0.2
2,857,786 + -0.1
2,857,786 + -0.5
2,857,786 + +0.3
2,857,787 - -1.4
2,857,797 + +1.2
2,857,939 + Pf6N2E2_2407 0.23 -0.1
2,857,939 + Pf6N2E2_2407 0.23 +1.1
2,857,940 - Pf6N2E2_2407 0.23 +0.7
2,857,940 - Pf6N2E2_2407 0.23 +0.3
2,857,940 - Pf6N2E2_2407 0.23 +1.5
2,857,942 - Pf6N2E2_2407 0.23 -1.1
2,857,942 - Pf6N2E2_2407 0.23 +0.5
2,857,989 + Pf6N2E2_2407 0.31 +0.5
2,857,990 - Pf6N2E2_2407 0.31 -1.1
2,857,990 - Pf6N2E2_2407 0.31 -0.7
2,857,990 - Pf6N2E2_2407 0.31 -0.1
2,857,994 - Pf6N2E2_2407 0.32 -0.1
2,857,994 - Pf6N2E2_2407 0.32 -1.5
2,858,049 + Pf6N2E2_2407 0.40 -0.1
2,858,050 - Pf6N2E2_2407 0.41 -0.7
2,858,050 - Pf6N2E2_2407 0.41 +0.4
2,858,435 - -0.2
2,858,495 + +0.1
2,858,656 + -0.2
2,858,656 + -0.1
2,858,657 - +0.1
2,858,696 + -0.3
2,858,696 + +0.8
2,858,697 - +0.9
2,858,697 - -0.1

Or see this region's nucleotide sequence