Experiment: 10% P. fluorescens FW300-N2C3 spent media from growth in 20 mM putrescine
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt Pf6N2E2_2405 and Pf6N2E2_2406 are separated by 70 nucleotides Pf6N2E2_2406 and Pf6N2E2_2407 are separated by 97 nucleotides Pf6N2E2_2407 and Pf6N2E2_2408 are separated by 180 nucleotides
Pf6N2E2_2405 - Hypothetical protein PvdY @ Siderophore synthetase small component, acetyltransferase, at 2,855,817 to 2,856,830
_2405
Pf6N2E2_2406 - Periplasmic binding protein, at 2,856,901 to 2,857,698
_2406
Pf6N2E2_2407 - Acyclic terpenes utilization regulator AtuR, TetR family, at 2,857,796 to 2,858,422
_2407
Pf6N2E2_2408 - 3-hydroxy-3isohexenylglutaryl-CoA:acetate lyase, at 2,858,603 to 2,860,408
_2408
Position (kb)
2856
2857
2858 Strain fitness (log2 ratio)
-2
-1
0
1
2 at 2856.004 kb on + strand, within Pf6N2E2_2405 at 2856.004 kb on + strand, within Pf6N2E2_2405 at 2856.005 kb on - strand, within Pf6N2E2_2405 at 2856.091 kb on + strand, within Pf6N2E2_2405 at 2856.091 kb on + strand, within Pf6N2E2_2405 at 2856.092 kb on - strand, within Pf6N2E2_2405 at 2856.092 kb on - strand, within Pf6N2E2_2405 at 2856.107 kb on - strand, within Pf6N2E2_2405 at 2856.139 kb on + strand, within Pf6N2E2_2405 at 2856.140 kb on - strand, within Pf6N2E2_2405 at 2856.140 kb on - strand, within Pf6N2E2_2405 at 2856.151 kb on + strand, within Pf6N2E2_2405 at 2856.152 kb on - strand, within Pf6N2E2_2405 at 2856.223 kb on + strand, within Pf6N2E2_2405 at 2856.224 kb on - strand, within Pf6N2E2_2405 at 2856.483 kb on + strand, within Pf6N2E2_2405 at 2856.484 kb on - strand, within Pf6N2E2_2405 at 2856.629 kb on - strand, within Pf6N2E2_2405 at 2856.845 kb on + strand at 2857.046 kb on + strand, within Pf6N2E2_2406 at 2857.047 kb on - strand, within Pf6N2E2_2406 at 2857.109 kb on + strand, within Pf6N2E2_2406 at 2857.109 kb on + strand, within Pf6N2E2_2406 at 2857.109 kb on + strand, within Pf6N2E2_2406 at 2857.109 kb on + strand, within Pf6N2E2_2406 at 2857.110 kb on - strand, within Pf6N2E2_2406 at 2857.110 kb on - strand, within Pf6N2E2_2406 at 2857.110 kb on - strand, within Pf6N2E2_2406 at 2857.110 kb on - strand, within Pf6N2E2_2406 at 2857.122 kb on - strand, within Pf6N2E2_2406 at 2857.190 kb on + strand, within Pf6N2E2_2406 at 2857.190 kb on + strand, within Pf6N2E2_2406 at 2857.190 kb on + strand, within Pf6N2E2_2406 at 2857.191 kb on - strand, within Pf6N2E2_2406 at 2857.191 kb on - strand, within Pf6N2E2_2406 at 2857.191 kb on - strand, within Pf6N2E2_2406 at 2857.208 kb on + strand, within Pf6N2E2_2406 at 2857.208 kb on + strand, within Pf6N2E2_2406 at 2857.208 kb on + strand, within Pf6N2E2_2406 at 2857.209 kb on - strand, within Pf6N2E2_2406 at 2857.209 kb on - strand, within Pf6N2E2_2406 at 2857.209 kb on - strand, within Pf6N2E2_2406 at 2857.209 kb on - strand, within Pf6N2E2_2406 at 2857.257 kb on - strand, within Pf6N2E2_2406 at 2857.257 kb on - strand, within Pf6N2E2_2406 at 2857.576 kb on - strand, within Pf6N2E2_2406 at 2857.576 kb on - strand, within Pf6N2E2_2406 at 2857.577 kb on + strand, within Pf6N2E2_2406 at 2857.578 kb on - strand, within Pf6N2E2_2406 at 2857.578 kb on - strand, within Pf6N2E2_2406 at 2857.578 kb on - strand, within Pf6N2E2_2406 at 2857.578 kb on - strand, within Pf6N2E2_2406 at 2857.578 kb on - strand, within Pf6N2E2_2406 at 2857.729 kb on + strand at 2857.730 kb on - strand at 2857.742 kb on - strand at 2857.759 kb on + strand at 2857.760 kb on - strand at 2857.781 kb on + strand at 2857.782 kb on - strand at 2857.782 kb on - strand at 2857.782 kb on - strand at 2857.782 kb on - strand at 2857.786 kb on + strand at 2857.786 kb on + strand at 2857.786 kb on + strand at 2857.786 kb on + strand at 2857.787 kb on - strand at 2857.797 kb on + strand at 2857.939 kb on + strand, within Pf6N2E2_2407 at 2857.939 kb on + strand, within Pf6N2E2_2407 at 2857.940 kb on - strand, within Pf6N2E2_2407 at 2857.940 kb on - strand, within Pf6N2E2_2407 at 2857.940 kb on - strand, within Pf6N2E2_2407 at 2857.942 kb on - strand, within Pf6N2E2_2407 at 2857.942 kb on - strand, within Pf6N2E2_2407 at 2857.989 kb on + strand, within Pf6N2E2_2407 at 2857.990 kb on - strand, within Pf6N2E2_2407 at 2857.990 kb on - strand, within Pf6N2E2_2407 at 2857.990 kb on - strand, within Pf6N2E2_2407 at 2857.994 kb on - strand, within Pf6N2E2_2407 at 2857.994 kb on - strand, within Pf6N2E2_2407 at 2858.049 kb on + strand, within Pf6N2E2_2407 at 2858.050 kb on - strand, within Pf6N2E2_2407 at 2858.050 kb on - strand, within Pf6N2E2_2407 at 2858.435 kb on - strand at 2858.495 kb on + strand at 2858.656 kb on + strand at 2858.656 kb on + strand at 2858.657 kb on - strand at 2858.696 kb on + strand at 2858.696 kb on + strand at 2858.697 kb on - strand at 2858.697 kb on - strand
Per-strain Table
Position Strand Gene LocusTag Fraction 10% P. fluorescens FW300-N2C3 spent media from growth in 20 mM putrescine remove 2,856,004 + Pf6N2E2_2405 0.18 +0.4 2,856,004 + Pf6N2E2_2405 0.18 -0.1 2,856,005 - Pf6N2E2_2405 0.19 -0.5 2,856,091 + Pf6N2E2_2405 0.27 -1.1 2,856,091 + Pf6N2E2_2405 0.27 -0.8 2,856,092 - Pf6N2E2_2405 0.27 -0.1 2,856,092 - Pf6N2E2_2405 0.27 +0.3 2,856,107 - Pf6N2E2_2405 0.29 +0.5 2,856,139 + Pf6N2E2_2405 0.32 -1.3 2,856,140 - Pf6N2E2_2405 0.32 +0.1 2,856,140 - Pf6N2E2_2405 0.32 +0.3 2,856,151 + Pf6N2E2_2405 0.33 -0.4 2,856,152 - Pf6N2E2_2405 0.33 -0.1 2,856,223 + Pf6N2E2_2405 0.40 +0.4 2,856,224 - Pf6N2E2_2405 0.40 +1.9 2,856,483 + Pf6N2E2_2405 0.66 -1.1 2,856,484 - Pf6N2E2_2405 0.66 -0.4 2,856,629 - Pf6N2E2_2405 0.80 +0.5 2,856,845 + -1.3 2,857,046 + Pf6N2E2_2406 0.18 +0.3 2,857,047 - Pf6N2E2_2406 0.18 +0.9 2,857,109 + Pf6N2E2_2406 0.26 +0.9 2,857,109 + Pf6N2E2_2406 0.26 -0.1 2,857,109 + Pf6N2E2_2406 0.26 +1.7 2,857,109 + Pf6N2E2_2406 0.26 +0.9 2,857,110 - Pf6N2E2_2406 0.26 +0.6 2,857,110 - Pf6N2E2_2406 0.26 +0.5 2,857,110 - Pf6N2E2_2406 0.26 -0.1 2,857,110 - Pf6N2E2_2406 0.26 -0.1 2,857,122 - Pf6N2E2_2406 0.28 +0.9 2,857,190 + Pf6N2E2_2406 0.36 +0.9 2,857,190 + Pf6N2E2_2406 0.36 +1.2 2,857,190 + Pf6N2E2_2406 0.36 -0.1 2,857,191 - Pf6N2E2_2406 0.36 -0.1 2,857,191 - Pf6N2E2_2406 0.36 +1.6 2,857,191 - Pf6N2E2_2406 0.36 -0.1 2,857,208 + Pf6N2E2_2406 0.38 +1.5 2,857,208 + Pf6N2E2_2406 0.38 +1.4 2,857,208 + Pf6N2E2_2406 0.38 +0.7 2,857,209 - Pf6N2E2_2406 0.39 +1.5 2,857,209 - Pf6N2E2_2406 0.39 +1.1 2,857,209 - Pf6N2E2_2406 0.39 +0.6 2,857,209 - Pf6N2E2_2406 0.39 -0.1 2,857,257 - Pf6N2E2_2406 0.45 +0.5 2,857,257 - Pf6N2E2_2406 0.45 +0.6 2,857,576 - Pf6N2E2_2406 0.85 +0.7 2,857,576 - Pf6N2E2_2406 0.85 -1.1 2,857,577 + Pf6N2E2_2406 0.85 +0.0 2,857,578 - Pf6N2E2_2406 0.85 -0.0 2,857,578 - Pf6N2E2_2406 0.85 -0.1 2,857,578 - Pf6N2E2_2406 0.85 -0.1 2,857,578 - Pf6N2E2_2406 0.85 +0.9 2,857,578 - Pf6N2E2_2406 0.85 +0.7 2,857,729 + +1.0 2,857,730 - -0.1 2,857,742 - +0.4 2,857,759 + -0.2 2,857,760 - -1.1 2,857,781 + -0.1 2,857,782 - -0.4 2,857,782 - -0.1 2,857,782 - -2.1 2,857,782 - -0.5 2,857,786 + +0.2 2,857,786 + -0.1 2,857,786 + -0.5 2,857,786 + +0.3 2,857,787 - -1.4 2,857,797 + +1.2 2,857,939 + Pf6N2E2_2407 0.23 -0.1 2,857,939 + Pf6N2E2_2407 0.23 +1.1 2,857,940 - Pf6N2E2_2407 0.23 +0.7 2,857,940 - Pf6N2E2_2407 0.23 +0.3 2,857,940 - Pf6N2E2_2407 0.23 +1.5 2,857,942 - Pf6N2E2_2407 0.23 -1.1 2,857,942 - Pf6N2E2_2407 0.23 +0.5 2,857,989 + Pf6N2E2_2407 0.31 +0.5 2,857,990 - Pf6N2E2_2407 0.31 -1.1 2,857,990 - Pf6N2E2_2407 0.31 -0.7 2,857,990 - Pf6N2E2_2407 0.31 -0.1 2,857,994 - Pf6N2E2_2407 0.32 -0.1 2,857,994 - Pf6N2E2_2407 0.32 -1.5 2,858,049 + Pf6N2E2_2407 0.40 -0.1 2,858,050 - Pf6N2E2_2407 0.41 -0.7 2,858,050 - Pf6N2E2_2407 0.41 +0.4 2,858,435 - -0.2 2,858,495 + +0.1 2,858,656 + -0.2 2,858,656 + -0.1 2,858,657 - +0.1 2,858,696 + -0.3 2,858,696 + +0.8 2,858,697 - +0.9 2,858,697 - -0.1
Or see this region's nucleotide sequence