Strain Fitness in Pseudomonas fluorescens FW300-N2E2 around Pf6N2E2_2370

Experiment: 10% P. fluorescens FW300-N2C3 spent media from growth in 20 mM putrescine

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Only strains with sufficient reads to estimate fitness are shown, but the strain fitness values are still rather noisy and may be biased towards zero. Strains near the edge of a gene are not shown as being associated with that gene (they are in grey). Strains in the central 10-90% of a gene are color coded by the insertion's strand. Usually, "+" means that the selectable marker is encoded on the forward strand, i.e., transcribed rightward.

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500 ntPf6N2E2_2369 and Pf6N2E2_2370 are separated by 59 nucleotidesPf6N2E2_2370 and Pf6N2E2_2371 overlap by 4 nucleotides Pf6N2E2_2369 - Flagellum-specific ATP synthase FliI, at 2,802,306 to 2,803,556 _2369 Pf6N2E2_2370 - Type III secretion inner membrane channel protein (LcrD,HrcV,EscV,SsaV), at 2,803,616 to 2,805,673 _2370 Pf6N2E2_2371 - Type III secretion outermembrane contact sensing protein (YopN,Yop4b,LcrE), at 2,805,670 to 2,806,773 _2371 Position (kb) 2803 2804 2805 2806Strain fitness (log2 ratio) -1 0 1 2at 2802.616 kb on + strand, within Pf6N2E2_2369at 2802.617 kb on - strand, within Pf6N2E2_2369at 2802.694 kb on + strand, within Pf6N2E2_2369at 2802.695 kb on - strand, within Pf6N2E2_2369at 2802.934 kb on - strand, within Pf6N2E2_2369at 2803.158 kb on + strand, within Pf6N2E2_2369at 2803.159 kb on - strand, within Pf6N2E2_2369at 2803.533 kb on + strandat 2803.533 kb on + strandat 2803.534 kb on - strandat 2803.590 kb on - strandat 2803.590 kb on - strandat 2803.944 kb on + strand, within Pf6N2E2_2370at 2804.112 kb on + strand, within Pf6N2E2_2370at 2804.260 kb on - strand, within Pf6N2E2_2370at 2804.505 kb on + strand, within Pf6N2E2_2370at 2804.729 kb on + strand, within Pf6N2E2_2370at 2804.730 kb on - strand, within Pf6N2E2_2370at 2804.980 kb on - strand, within Pf6N2E2_2370at 2805.059 kb on - strand, within Pf6N2E2_2370at 2805.059 kb on - strand, within Pf6N2E2_2370at 2805.120 kb on + strand, within Pf6N2E2_2370at 2805.121 kb on - strand, within Pf6N2E2_2370at 2805.499 kb on - strandat 2805.499 kb on - strandat 2805.717 kb on - strandat 2805.908 kb on + strand, within Pf6N2E2_2371at 2806.119 kb on + strand, within Pf6N2E2_2371at 2806.120 kb on - strand, within Pf6N2E2_2371

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Per-strain Table

Position Strand Gene LocusTag Fraction 10% P. fluorescens FW300-N2C3 spent media from growth in 20 mM putrescine
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2,802,616 + Pf6N2E2_2369 0.25 -0.1
2,802,617 - Pf6N2E2_2369 0.25 +0.3
2,802,694 + Pf6N2E2_2369 0.31 -0.4
2,802,695 - Pf6N2E2_2369 0.31 +0.2
2,802,934 - Pf6N2E2_2369 0.50 -0.1
2,803,158 + Pf6N2E2_2369 0.68 +0.5
2,803,159 - Pf6N2E2_2369 0.68 +1.1
2,803,533 + -0.1
2,803,533 + +0.2
2,803,534 - -0.3
2,803,590 - +1.1
2,803,590 - +0.3
2,803,944 + Pf6N2E2_2370 0.16 -0.1
2,804,112 + Pf6N2E2_2370 0.24 +1.1
2,804,260 - Pf6N2E2_2370 0.31 +0.9
2,804,505 + Pf6N2E2_2370 0.43 +1.2
2,804,729 + Pf6N2E2_2370 0.54 +0.3
2,804,730 - Pf6N2E2_2370 0.54 +0.6
2,804,980 - Pf6N2E2_2370 0.66 +0.5
2,805,059 - Pf6N2E2_2370 0.70 +0.6
2,805,059 - Pf6N2E2_2370 0.70 +0.9
2,805,120 + Pf6N2E2_2370 0.73 +0.7
2,805,121 - Pf6N2E2_2370 0.73 +2.7
2,805,499 - +1.3
2,805,499 - +0.5
2,805,717 - -0.3
2,805,908 + Pf6N2E2_2371 0.22 -0.1
2,806,119 + Pf6N2E2_2371 0.41 -0.5
2,806,120 - Pf6N2E2_2371 0.41 +0.6

Or see this region's nucleotide sequence