Experiment: 10% P. fluorescens FW300-N2C3 spent media from growth in 20 mM putrescine
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt Pf6N2E2_2282 and Pf6N2E2_2283 are separated by 241 nucleotides Pf6N2E2_2283 and Pf6N2E2_2284 are separated by 10 nucleotides Pf6N2E2_2284 and Pf6N2E2_2285 are separated by 71 nucleotides Pf6N2E2_2285 and Pf6N2E2_2286 are separated by 106 nucleotides
Pf6N2E2_2282 - Predicted transcriptional regulator for fatty acid degradation FadQ, TetR family, at 2,701,757 to 2,702,464
_2282
Pf6N2E2_2283 - SOS-response repressor and protease LexA (EC 3.4.21.88), at 2,702,706 to 2,703,314
_2283
Pf6N2E2_2284 - Cell division inhibitor-related protein, at 2,703,325 to 2,703,798
_2284
Pf6N2E2_2285 - FIG00954079: hypothetical protein, at 2,703,870 to 2,704,103
_2285
Pf6N2E2_2286 - FIG00953287: hypothetical protein, at 2,704,210 to 2,704,734
_2286
Position (kb)
2703
2704 Strain fitness (log2 ratio)
-2
-1
0
1
2 at 2702.335 kb on - strand, within Pf6N2E2_2282 at 2702.499 kb on + strand at 2702.500 kb on - strand at 2702.500 kb on - strand at 2702.558 kb on + strand at 2702.559 kb on - strand at 2702.559 kb on - strand at 2702.559 kb on - strand at 2702.559 kb on - strand at 2702.563 kb on - strand at 2702.585 kb on + strand at 2702.586 kb on - strand at 2702.586 kb on - strand at 2702.586 kb on - strand at 2702.665 kb on - strand at 2702.665 kb on - strand at 2702.705 kb on + strand at 2702.710 kb on + strand at 2703.324 kb on + strand at 2703.465 kb on - strand, within Pf6N2E2_2284 at 2703.547 kb on - strand, within Pf6N2E2_2284 at 2703.547 kb on - strand, within Pf6N2E2_2284 at 2703.547 kb on - strand, within Pf6N2E2_2284 at 2703.784 kb on - strand at 2703.990 kb on + strand, within Pf6N2E2_2285 at 2703.991 kb on - strand, within Pf6N2E2_2285 at 2704.086 kb on + strand at 2704.097 kb on + strand at 2704.097 kb on + strand at 2704.098 kb on - strand at 2704.098 kb on - strand at 2704.103 kb on + strand at 2704.104 kb on - strand at 2704.104 kb on - strand at 2704.104 kb on - strand at 2704.104 kb on - strand at 2704.104 kb on - strand at 2704.134 kb on + strand at 2704.134 kb on + strand at 2704.134 kb on + strand at 2704.135 kb on - strand at 2704.135 kb on - strand at 2704.135 kb on - strand at 2704.135 kb on - strand at 2704.135 kb on - strand at 2704.135 kb on - strand at 2704.135 kb on - strand at 2704.135 kb on - strand at 2704.135 kb on - strand at 2704.135 kb on - strand at 2704.135 kb on - strand at 2704.136 kb on + strand at 2704.136 kb on + strand at 2704.136 kb on + strand at 2704.136 kb on + strand at 2704.136 kb on + strand at 2704.136 kb on + strand at 2704.136 kb on + strand at 2704.136 kb on + strand at 2704.137 kb on - strand at 2704.137 kb on - strand at 2704.137 kb on - strand at 2704.137 kb on - strand at 2704.137 kb on - strand at 2704.137 kb on - strand at 2704.137 kb on - strand at 2704.137 kb on - strand at 2704.137 kb on - strand at 2704.137 kb on - strand at 2704.137 kb on - strand at 2704.137 kb on - strand at 2704.137 kb on - strand at 2704.137 kb on - strand at 2704.138 kb on + strand at 2704.138 kb on + strand at 2704.138 kb on + strand at 2704.138 kb on + strand at 2704.139 kb on - strand at 2704.139 kb on - strand at 2704.192 kb on - strand at 2704.545 kb on - strand, within Pf6N2E2_2286 at 2704.586 kb on + strand, within Pf6N2E2_2286 at 2704.586 kb on + strand, within Pf6N2E2_2286 at 2704.587 kb on - strand, within Pf6N2E2_2286 at 2704.694 kb on + strand at 2704.718 kb on + strand at 2704.718 kb on + strand at 2704.718 kb on + strand at 2704.719 kb on - strand at 2704.719 kb on - strand at 2704.719 kb on - strand
Per-strain Table
Position Strand Gene LocusTag Fraction 10% P. fluorescens FW300-N2C3 spent media from growth in 20 mM putrescine remove 2,702,335 - Pf6N2E2_2282 0.82 -0.1 2,702,499 + -0.1 2,702,500 - +1.4 2,702,500 - +0.4 2,702,558 + +0.9 2,702,559 - -0.6 2,702,559 - +1.4 2,702,559 - +0.4 2,702,559 - +0.9 2,702,563 - +1.3 2,702,585 + -0.1 2,702,586 - -0.5 2,702,586 - -1.0 2,702,586 - -0.1 2,702,665 - +0.5 2,702,665 - +0.9 2,702,705 + -0.1 2,702,710 + -2.1 2,703,324 + -0.1 2,703,465 - Pf6N2E2_2284 0.30 -1.7 2,703,547 - Pf6N2E2_2284 0.47 -0.6 2,703,547 - Pf6N2E2_2284 0.47 -0.4 2,703,547 - Pf6N2E2_2284 0.47 -0.3 2,703,784 - -0.1 2,703,990 + Pf6N2E2_2285 0.51 +0.5 2,703,991 - Pf6N2E2_2285 0.52 -0.3 2,704,086 + +0.9 2,704,097 + -0.1 2,704,097 + +1.2 2,704,098 - +1.1 2,704,098 - -0.1 2,704,103 + -0.5 2,704,104 - +1.5 2,704,104 - -0.1 2,704,104 - -0.2 2,704,104 - +0.5 2,704,104 - -0.1 2,704,134 + +1.7 2,704,134 + -0.1 2,704,134 + +0.9 2,704,135 - +0.9 2,704,135 - +2.5 2,704,135 - -0.1 2,704,135 - -1.5 2,704,135 - -0.1 2,704,135 - +0.2 2,704,135 - +0.3 2,704,135 - -0.0 2,704,135 - -0.1 2,704,135 - +1.8 2,704,135 - -1.1 2,704,136 + -0.1 2,704,136 + +0.4 2,704,136 + +0.5 2,704,136 + +1.5 2,704,136 + -0.1 2,704,136 + -0.1 2,704,136 + -0.1 2,704,136 + -0.1 2,704,137 - -0.3 2,704,137 - +1.1 2,704,137 - -0.1 2,704,137 - +0.2 2,704,137 - -0.4 2,704,137 - -0.8 2,704,137 - +0.3 2,704,137 - +0.9 2,704,137 - -1.1 2,704,137 - +0.9 2,704,137 - +1.0 2,704,137 - -0.1 2,704,137 - -0.1 2,704,137 - +0.4 2,704,138 + +0.1 2,704,138 + -0.1 2,704,138 + -0.1 2,704,138 + +2.0 2,704,139 - +0.5 2,704,139 - +0.6 2,704,192 - -1.4 2,704,545 - Pf6N2E2_2286 0.64 -0.0 2,704,586 + Pf6N2E2_2286 0.72 -0.9 2,704,586 + Pf6N2E2_2286 0.72 +1.5 2,704,587 - Pf6N2E2_2286 0.72 -0.3 2,704,694 + +0.2 2,704,718 + +0.5 2,704,718 + -0.3 2,704,718 + -1.3 2,704,719 - -0.1 2,704,719 - +0.2 2,704,719 - -0.1
Or see this region's nucleotide sequence