Experiment: 10% P. fluorescens FW300-N2C3 spent media from growth in 20 mM putrescine
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt Pf6N2E2_1843 and Pf6N2E2_1844 are separated by 201 nucleotides Pf6N2E2_1844 and Pf6N2E2_1845 are separated by 235 nucleotides
Pf6N2E2_1843 - Dehydrogenases with different specificities (related to short-chain alcohol dehydrogenases), at 2,180,780 to 2,181,541
_1843
Pf6N2E2_1844 - MaoC-related acyl dehydratase, at 2,181,743 to 2,182,228
_1844
Pf6N2E2_1845 - FIG005548: transport protein, at 2,182,464 to 2,184,959
_1845
Position (kb)
2181
2182
2183 Strain fitness (log2 ratio)
-2
-1
0
1
2 at 2180.937 kb on + strand, within Pf6N2E2_1843 at 2181.173 kb on + strand, within Pf6N2E2_1843 at 2181.174 kb on - strand, within Pf6N2E2_1843 at 2181.206 kb on + strand, within Pf6N2E2_1843 at 2181.206 kb on + strand, within Pf6N2E2_1843 at 2181.206 kb on + strand, within Pf6N2E2_1843 at 2181.207 kb on - strand, within Pf6N2E2_1843 at 2181.207 kb on - strand, within Pf6N2E2_1843 at 2181.207 kb on - strand, within Pf6N2E2_1843 at 2181.207 kb on - strand, within Pf6N2E2_1843 at 2181.219 kb on - strand, within Pf6N2E2_1843 at 2181.249 kb on - strand, within Pf6N2E2_1843 at 2181.597 kb on + strand at 2181.598 kb on - strand at 2181.678 kb on + strand at 2181.716 kb on + strand at 2181.858 kb on - strand, within Pf6N2E2_1844 at 2181.876 kb on - strand, within Pf6N2E2_1844 at 2181.955 kb on - strand, within Pf6N2E2_1844 at 2182.083 kb on + strand, within Pf6N2E2_1844 at 2182.084 kb on - strand, within Pf6N2E2_1844 at 2182.084 kb on - strand, within Pf6N2E2_1844 at 2182.084 kb on - strand, within Pf6N2E2_1844 at 2182.151 kb on - strand, within Pf6N2E2_1844 at 2182.256 kb on - strand at 2182.325 kb on - strand at 2182.449 kb on + strand at 2182.450 kb on - strand at 2182.450 kb on - strand at 2182.563 kb on + strand at 2182.564 kb on - strand at 2182.587 kb on + strand at 2182.647 kb on - strand at 2182.707 kb on + strand at 2182.832 kb on - strand, within Pf6N2E2_1845 at 2182.840 kb on + strand, within Pf6N2E2_1845 at 2182.954 kb on - strand, within Pf6N2E2_1845 at 2182.954 kb on - strand, within Pf6N2E2_1845 at 2182.969 kb on - strand, within Pf6N2E2_1845 at 2182.969 kb on - strand, within Pf6N2E2_1845 at 2183.021 kb on - strand, within Pf6N2E2_1845 at 2183.126 kb on + strand, within Pf6N2E2_1845 at 2183.126 kb on + strand, within Pf6N2E2_1845 at 2183.126 kb on + strand, within Pf6N2E2_1845 at 2183.126 kb on + strand, within Pf6N2E2_1845 at 2183.127 kb on - strand, within Pf6N2E2_1845 at 2183.127 kb on - strand, within Pf6N2E2_1845 at 2183.127 kb on - strand, within Pf6N2E2_1845 at 2183.127 kb on - strand, within Pf6N2E2_1845 at 2183.127 kb on - strand, within Pf6N2E2_1845 at 2183.127 kb on - strand, within Pf6N2E2_1845 at 2183.183 kb on + strand, within Pf6N2E2_1845
Per-strain Table
Position Strand Gene LocusTag Fraction 10% P. fluorescens FW300-N2C3 spent media from growth in 20 mM putrescine remove 2,180,937 + Pf6N2E2_1843 0.21 -1.2 2,181,173 + Pf6N2E2_1843 0.52 -1.0 2,181,174 - Pf6N2E2_1843 0.52 -0.1 2,181,206 + Pf6N2E2_1843 0.56 -0.6 2,181,206 + Pf6N2E2_1843 0.56 -0.7 2,181,206 + Pf6N2E2_1843 0.56 +0.6 2,181,207 - Pf6N2E2_1843 0.56 +0.3 2,181,207 - Pf6N2E2_1843 0.56 +0.6 2,181,207 - Pf6N2E2_1843 0.56 -0.6 2,181,207 - Pf6N2E2_1843 0.56 -0.3 2,181,219 - Pf6N2E2_1843 0.58 -0.1 2,181,249 - Pf6N2E2_1843 0.62 +0.8 2,181,597 + -0.4 2,181,598 - +0.6 2,181,678 + -0.7 2,181,716 + -0.4 2,181,858 - Pf6N2E2_1844 0.24 -1.0 2,181,876 - Pf6N2E2_1844 0.27 +0.8 2,181,955 - Pf6N2E2_1844 0.44 +1.0 2,182,083 + Pf6N2E2_1844 0.70 +0.6 2,182,084 - Pf6N2E2_1844 0.70 -0.6 2,182,084 - Pf6N2E2_1844 0.70 +1.2 2,182,084 - Pf6N2E2_1844 0.70 +0.7 2,182,151 - Pf6N2E2_1844 0.84 -0.4 2,182,256 - +0.6 2,182,325 - +0.5 2,182,449 + -0.4 2,182,450 - +0.4 2,182,450 - -1.7 2,182,563 + +0.6 2,182,564 - -1.6 2,182,587 + -0.4 2,182,647 - -0.5 2,182,707 + +1.9 2,182,832 - Pf6N2E2_1845 0.15 -0.6 2,182,840 + Pf6N2E2_1845 0.15 -1.4 2,182,954 - Pf6N2E2_1845 0.20 +0.9 2,182,954 - Pf6N2E2_1845 0.20 -0.6 2,182,969 - Pf6N2E2_1845 0.20 -0.3 2,182,969 - Pf6N2E2_1845 0.20 +0.7 2,183,021 - Pf6N2E2_1845 0.22 -0.4 2,183,126 + Pf6N2E2_1845 0.27 +0.2 2,183,126 + Pf6N2E2_1845 0.27 +0.6 2,183,126 + Pf6N2E2_1845 0.27 -0.4 2,183,126 + Pf6N2E2_1845 0.27 -0.3 2,183,127 - Pf6N2E2_1845 0.27 +1.7 2,183,127 - Pf6N2E2_1845 0.27 -2.0 2,183,127 - Pf6N2E2_1845 0.27 -2.0 2,183,127 - Pf6N2E2_1845 0.27 -0.4 2,183,127 - Pf6N2E2_1845 0.27 -0.1 2,183,127 - Pf6N2E2_1845 0.27 +0.6 2,183,183 + Pf6N2E2_1845 0.29 +0.9
Or see this region's nucleotide sequence