Experiment: 10% P. fluorescens FW300-N2C3 spent media from growth in 20 mM putrescine
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt Pf6N2E2_1773 and Pf6N2E2_1774 overlap by 4 nucleotides Pf6N2E2_1774 and Pf6N2E2_1775 are separated by 15 nucleotides
Pf6N2E2_1773 - Sensory box histidine kinase, at 2,096,676 to 2,099,672
_1773
Pf6N2E2_1774 - DNA-binding response regulator, LuxR family, near polyamine transporter, at 2,099,669 to 2,100,298
_1774
Pf6N2E2_1775 - Chemotaxis response regulator protein-glutamate methylesterase CheB (EC 3.1.1.61), at 2,100,314 to 2,101,390
_1775
Position (kb)
2099
2100
2101 Strain fitness (log2 ratio)
-2
-1
0
1 at 2098.673 kb on + strand, within Pf6N2E2_1773 at 2098.674 kb on - strand, within Pf6N2E2_1773 at 2098.674 kb on - strand, within Pf6N2E2_1773 at 2098.807 kb on + strand, within Pf6N2E2_1773 at 2098.863 kb on + strand, within Pf6N2E2_1773 at 2098.864 kb on - strand, within Pf6N2E2_1773 at 2098.864 kb on - strand, within Pf6N2E2_1773 at 2098.864 kb on - strand, within Pf6N2E2_1773 at 2098.864 kb on - strand, within Pf6N2E2_1773 at 2098.864 kb on - strand, within Pf6N2E2_1773 at 2098.864 kb on - strand, within Pf6N2E2_1773 at 2098.864 kb on - strand, within Pf6N2E2_1773 at 2098.864 kb on - strand, within Pf6N2E2_1773 at 2098.864 kb on - strand, within Pf6N2E2_1773 at 2098.970 kb on + strand, within Pf6N2E2_1773 at 2098.970 kb on + strand, within Pf6N2E2_1773 at 2098.972 kb on + strand, within Pf6N2E2_1773 at 2098.972 kb on + strand, within Pf6N2E2_1773 at 2098.972 kb on + strand, within Pf6N2E2_1773 at 2098.972 kb on + strand, within Pf6N2E2_1773 at 2098.972 kb on + strand, within Pf6N2E2_1773 at 2098.972 kb on + strand, within Pf6N2E2_1773 at 2098.973 kb on - strand, within Pf6N2E2_1773 at 2098.973 kb on - strand, within Pf6N2E2_1773 at 2098.973 kb on - strand, within Pf6N2E2_1773 at 2098.973 kb on - strand, within Pf6N2E2_1773 at 2098.973 kb on - strand, within Pf6N2E2_1773 at 2098.973 kb on - strand, within Pf6N2E2_1773 at 2098.973 kb on - strand, within Pf6N2E2_1773 at 2099.154 kb on + strand, within Pf6N2E2_1773 at 2099.396 kb on + strand at 2099.397 kb on - strand at 2099.456 kb on + strand at 2099.821 kb on - strand, within Pf6N2E2_1774 at 2100.200 kb on - strand, within Pf6N2E2_1774 at 2100.418 kb on - strand at 2100.418 kb on - strand at 2100.418 kb on - strand at 2100.647 kb on + strand, within Pf6N2E2_1775 at 2100.648 kb on - strand, within Pf6N2E2_1775 at 2100.648 kb on - strand, within Pf6N2E2_1775 at 2100.653 kb on - strand, within Pf6N2E2_1775 at 2100.831 kb on - strand, within Pf6N2E2_1775 at 2100.831 kb on - strand, within Pf6N2E2_1775 at 2100.865 kb on + strand, within Pf6N2E2_1775 at 2100.866 kb on - strand, within Pf6N2E2_1775 at 2101.196 kb on - strand, within Pf6N2E2_1775 at 2101.273 kb on - strand, within Pf6N2E2_1775
Per-strain Table
Position Strand Gene LocusTag Fraction 10% P. fluorescens FW300-N2C3 spent media from growth in 20 mM putrescine remove 2,098,673 + Pf6N2E2_1773 0.67 +0.6 2,098,674 - Pf6N2E2_1773 0.67 +0.2 2,098,674 - Pf6N2E2_1773 0.67 +1.2 2,098,807 + Pf6N2E2_1773 0.71 +1.2 2,098,863 + Pf6N2E2_1773 0.73 -0.7 2,098,864 - Pf6N2E2_1773 0.73 -0.8 2,098,864 - Pf6N2E2_1773 0.73 -0.4 2,098,864 - Pf6N2E2_1773 0.73 -1.5 2,098,864 - Pf6N2E2_1773 0.73 +0.9 2,098,864 - Pf6N2E2_1773 0.73 -0.4 2,098,864 - Pf6N2E2_1773 0.73 -0.4 2,098,864 - Pf6N2E2_1773 0.73 -0.4 2,098,864 - Pf6N2E2_1773 0.73 +0.8 2,098,864 - Pf6N2E2_1773 0.73 -0.4 2,098,970 + Pf6N2E2_1773 0.77 -0.4 2,098,970 + Pf6N2E2_1773 0.77 -0.7 2,098,972 + Pf6N2E2_1773 0.77 -0.4 2,098,972 + Pf6N2E2_1773 0.77 +0.1 2,098,972 + Pf6N2E2_1773 0.77 -0.6 2,098,972 + Pf6N2E2_1773 0.77 +0.8 2,098,972 + Pf6N2E2_1773 0.77 -1.7 2,098,972 + Pf6N2E2_1773 0.77 -0.4 2,098,973 - Pf6N2E2_1773 0.77 -0.4 2,098,973 - Pf6N2E2_1773 0.77 +0.2 2,098,973 - Pf6N2E2_1773 0.77 -0.4 2,098,973 - Pf6N2E2_1773 0.77 -0.7 2,098,973 - Pf6N2E2_1773 0.77 +0.3 2,098,973 - Pf6N2E2_1773 0.77 -0.4 2,098,973 - Pf6N2E2_1773 0.77 +0.8 2,099,154 + Pf6N2E2_1773 0.83 -0.8 2,099,396 + -0.4 2,099,397 - +0.7 2,099,456 + -0.7 2,099,821 - Pf6N2E2_1774 0.24 -1.1 2,100,200 - Pf6N2E2_1774 0.84 -2.0 2,100,418 - +1.2 2,100,418 - +1.4 2,100,418 - -0.2 2,100,647 + Pf6N2E2_1775 0.31 -0.4 2,100,648 - Pf6N2E2_1775 0.31 +0.6 2,100,648 - Pf6N2E2_1775 0.31 +0.2 2,100,653 - Pf6N2E2_1775 0.31 -0.4 2,100,831 - Pf6N2E2_1775 0.48 +0.5 2,100,831 - Pf6N2E2_1775 0.48 +0.2 2,100,865 + Pf6N2E2_1775 0.51 -1.2 2,100,866 - Pf6N2E2_1775 0.51 -1.4 2,101,196 - Pf6N2E2_1775 0.82 -0.4 2,101,273 - Pf6N2E2_1775 0.89 +0.2
Or see this region's nucleotide sequence