Experiment: 10% P. fluorescens FW300-N2C3 spent media from growth in 20 mM putrescine
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt Pf6N2E2_1672 and Pf6N2E2_1673 are separated by 19 nucleotides Pf6N2E2_1673 and Pf6N2E2_1674 are separated by 608 nucleotides
Pf6N2E2_1672 - hypothetical protein, at 1,978,161 to 1,979,042
_1672
Pf6N2E2_1673 - Transcriptional regulator, TetR family, at 1,979,062 to 1,979,499
_1673
Pf6N2E2_1674 - Hydroxymethylglutaryl-CoA synthase (EC 2.3.3.10), at 1,980,108 to 1,981,190
_1674
Position (kb)
1979
1980 Strain fitness (log2 ratio)
-2
-1
0
1
2
3 at 1978.063 kb on + strand at 1978.063 kb on + strand at 1978.067 kb on + strand at 1978.067 kb on + strand at 1978.068 kb on - strand at 1978.068 kb on - strand at 1978.092 kb on + strand at 1978.093 kb on - strand at 1978.143 kb on + strand at 1978.143 kb on + strand at 1978.144 kb on - strand at 1978.144 kb on - strand at 1978.144 kb on - strand at 1978.154 kb on + strand at 1978.155 kb on - strand at 1978.155 kb on - strand at 1978.174 kb on + strand at 1978.191 kb on + strand at 1978.254 kb on + strand, within Pf6N2E2_1672 at 1978.408 kb on - strand, within Pf6N2E2_1672 at 1978.435 kb on - strand, within Pf6N2E2_1672 at 1978.435 kb on - strand, within Pf6N2E2_1672 at 1978.435 kb on - strand, within Pf6N2E2_1672 at 1978.435 kb on - strand, within Pf6N2E2_1672 at 1978.521 kb on + strand, within Pf6N2E2_1672 at 1978.540 kb on - strand, within Pf6N2E2_1672 at 1978.614 kb on + strand, within Pf6N2E2_1672 at 1978.615 kb on - strand, within Pf6N2E2_1672 at 1978.615 kb on - strand, within Pf6N2E2_1672 at 1978.615 kb on - strand, within Pf6N2E2_1672 at 1978.615 kb on - strand, within Pf6N2E2_1672 at 1978.622 kb on + strand, within Pf6N2E2_1672 at 1978.623 kb on - strand, within Pf6N2E2_1672 at 1978.623 kb on - strand, within Pf6N2E2_1672 at 1978.623 kb on - strand, within Pf6N2E2_1672 at 1978.623 kb on - strand, within Pf6N2E2_1672 at 1978.623 kb on - strand, within Pf6N2E2_1672 at 1978.623 kb on - strand, within Pf6N2E2_1672 at 1978.642 kb on - strand, within Pf6N2E2_1672 at 1978.642 kb on - strand, within Pf6N2E2_1672 at 1978.732 kb on + strand, within Pf6N2E2_1672 at 1978.733 kb on - strand, within Pf6N2E2_1672 at 1978.780 kb on + strand, within Pf6N2E2_1672 at 1978.780 kb on + strand, within Pf6N2E2_1672 at 1978.780 kb on + strand, within Pf6N2E2_1672 at 1978.830 kb on + strand, within Pf6N2E2_1672 at 1978.831 kb on - strand, within Pf6N2E2_1672 at 1978.906 kb on + strand, within Pf6N2E2_1672 at 1978.907 kb on - strand, within Pf6N2E2_1672 at 1979.040 kb on + strand at 1979.040 kb on + strand at 1979.227 kb on - strand, within Pf6N2E2_1673 at 1979.227 kb on - strand, within Pf6N2E2_1673 at 1979.446 kb on - strand, within Pf6N2E2_1673 at 1979.483 kb on + strand at 1979.484 kb on - strand at 1979.525 kb on + strand at 1979.526 kb on - strand at 1979.598 kb on + strand at 1979.599 kb on - strand at 1979.599 kb on - strand at 1979.599 kb on - strand at 1979.599 kb on - strand at 1979.608 kb on + strand at 1979.609 kb on - strand at 1979.688 kb on + strand at 1979.689 kb on - strand at 1979.778 kb on + strand at 1979.781 kb on - strand at 1979.781 kb on - strand at 1979.785 kb on - strand at 1979.785 kb on - strand at 1979.785 kb on - strand at 1979.787 kb on - strand at 1979.787 kb on - strand at 1979.828 kb on + strand at 1979.829 kb on - strand at 1979.829 kb on - strand at 1979.829 kb on - strand at 1979.842 kb on - strand at 1979.849 kb on + strand at 1979.849 kb on + strand at 1979.850 kb on - strand at 1979.910 kb on + strand at 1979.910 kb on + strand at 1979.914 kb on + strand at 1979.937 kb on + strand at 1979.986 kb on + strand at 1980.008 kb on + strand at 1980.043 kb on + strand at 1980.103 kb on + strand at 1980.103 kb on + strand at 1980.103 kb on + strand at 1980.103 kb on + strand at 1980.103 kb on + strand at 1980.103 kb on + strand at 1980.104 kb on - strand at 1980.113 kb on + strand at 1980.113 kb on + strand at 1980.113 kb on + strand at 1980.113 kb on + strand at 1980.113 kb on + strand at 1980.113 kb on + strand at 1980.114 kb on - strand at 1980.118 kb on + strand at 1980.132 kb on + strand at 1980.211 kb on + strand at 1980.211 kb on + strand at 1980.269 kb on + strand, within Pf6N2E2_1674 at 1980.269 kb on + strand, within Pf6N2E2_1674 at 1980.269 kb on + strand, within Pf6N2E2_1674 at 1980.360 kb on + strand, within Pf6N2E2_1674 at 1980.360 kb on + strand, within Pf6N2E2_1674 at 1980.360 kb on + strand, within Pf6N2E2_1674 at 1980.361 kb on - strand, within Pf6N2E2_1674 at 1980.477 kb on + strand, within Pf6N2E2_1674 at 1980.487 kb on + strand, within Pf6N2E2_1674
Per-strain Table
Position Strand Gene LocusTag Fraction 10% P. fluorescens FW300-N2C3 spent media from growth in 20 mM putrescine remove 1,978,063 + -0.5 1,978,063 + -0.3 1,978,067 + -1.3 1,978,067 + +0.3 1,978,068 - +0.5 1,978,068 - -0.5 1,978,092 + +0.2 1,978,093 - +3.1 1,978,143 + +0.1 1,978,143 + -0.4 1,978,144 - +1.1 1,978,144 - -0.2 1,978,144 - +1.4 1,978,154 + +0.9 1,978,155 - +0.1 1,978,155 - -0.5 1,978,174 + -1.5 1,978,191 + -0.5 1,978,254 + Pf6N2E2_1672 0.11 -2.1 1,978,408 - Pf6N2E2_1672 0.28 -0.5 1,978,435 - Pf6N2E2_1672 0.31 -0.1 1,978,435 - Pf6N2E2_1672 0.31 +0.3 1,978,435 - Pf6N2E2_1672 0.31 -0.1 1,978,435 - Pf6N2E2_1672 0.31 +1.1 1,978,521 + Pf6N2E2_1672 0.41 -0.5 1,978,540 - Pf6N2E2_1672 0.43 -0.9 1,978,614 + Pf6N2E2_1672 0.51 +0.5 1,978,615 - Pf6N2E2_1672 0.51 -1.1 1,978,615 - Pf6N2E2_1672 0.51 +1.5 1,978,615 - Pf6N2E2_1672 0.51 -0.1 1,978,615 - Pf6N2E2_1672 0.51 +1.8 1,978,622 + Pf6N2E2_1672 0.52 +0.5 1,978,623 - Pf6N2E2_1672 0.52 +1.4 1,978,623 - Pf6N2E2_1672 0.52 +1.7 1,978,623 - Pf6N2E2_1672 0.52 +1.3 1,978,623 - Pf6N2E2_1672 0.52 -0.5 1,978,623 - Pf6N2E2_1672 0.52 -0.1 1,978,623 - Pf6N2E2_1672 0.52 +0.5 1,978,642 - Pf6N2E2_1672 0.55 +0.7 1,978,642 - Pf6N2E2_1672 0.55 +1.1 1,978,732 + Pf6N2E2_1672 0.65 -0.5 1,978,733 - Pf6N2E2_1672 0.65 +2.1 1,978,780 + Pf6N2E2_1672 0.70 -0.8 1,978,780 + Pf6N2E2_1672 0.70 -1.1 1,978,780 + Pf6N2E2_1672 0.70 +0.9 1,978,830 + Pf6N2E2_1672 0.76 -0.0 1,978,831 - Pf6N2E2_1672 0.76 -0.4 1,978,906 + Pf6N2E2_1672 0.84 +0.0 1,978,907 - Pf6N2E2_1672 0.85 +0.0 1,979,040 + +0.4 1,979,040 + +0.5 1,979,227 - Pf6N2E2_1673 0.38 -1.2 1,979,227 - Pf6N2E2_1673 0.38 +0.1 1,979,446 - Pf6N2E2_1673 0.88 -1.0 1,979,483 + +0.5 1,979,484 - -1.1 1,979,525 + -0.9 1,979,526 - +0.4 1,979,598 + -0.5 1,979,599 - +0.2 1,979,599 - +0.4 1,979,599 - +0.2 1,979,599 - -0.3 1,979,608 + -1.1 1,979,609 - +0.9 1,979,688 + -1.1 1,979,689 - +0.3 1,979,778 + -0.5 1,979,781 - -0.5 1,979,781 - +0.3 1,979,785 - +0.3 1,979,785 - -0.0 1,979,785 - -0.3 1,979,787 - +0.1 1,979,787 - +0.6 1,979,828 + -1.3 1,979,829 - +0.4 1,979,829 - +0.9 1,979,829 - -0.1 1,979,842 - -0.9 1,979,849 + +1.0 1,979,849 + +0.5 1,979,850 - -0.5 1,979,910 + -0.2 1,979,910 + +0.4 1,979,914 + -0.2 1,979,937 + +0.7 1,979,986 + +1.1 1,980,008 + -0.3 1,980,043 + +0.2 1,980,103 + +0.7 1,980,103 + -0.2 1,980,103 + +0.5 1,980,103 + +0.7 1,980,103 + -0.2 1,980,103 + -0.5 1,980,104 - +0.1 1,980,113 + +0.2 1,980,113 + -0.1 1,980,113 + -0.1 1,980,113 + +0.3 1,980,113 + +0.1 1,980,113 + +0.3 1,980,114 - +0.5 1,980,118 + -0.2 1,980,132 + +0.7 1,980,211 + +0.4 1,980,211 + -0.2 1,980,269 + Pf6N2E2_1674 0.15 -2.3 1,980,269 + Pf6N2E2_1674 0.15 +0.5 1,980,269 + Pf6N2E2_1674 0.15 +0.1 1,980,360 + Pf6N2E2_1674 0.23 -0.5 1,980,360 + Pf6N2E2_1674 0.23 -0.9 1,980,360 + Pf6N2E2_1674 0.23 +0.3 1,980,361 - Pf6N2E2_1674 0.23 +0.1 1,980,477 + Pf6N2E2_1674 0.34 +0.8 1,980,487 + Pf6N2E2_1674 0.35 -0.4
Or see this region's nucleotide sequence