Experiment: 10% P. fluorescens FW300-N2C3 spent media from growth in 20 mM putrescine
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt Pf6N2E2_1564 and Pf6N2E2_1565 overlap by 4 nucleotides Pf6N2E2_1565 and Pf6N2E2_1566 overlap by 4 nucleotides Pf6N2E2_1566 and Pf6N2E2_1567 are separated by 39 nucleotides Pf6N2E2_1567 and Pf6N2E2_1568 overlap by 4 nucleotides
Pf6N2E2_1564 - 3-dehydroquinate synthase (EC 4.2.3.4), at 1,825,739 to 1,827,613
_1564
Pf6N2E2_1565 - FIG00961164: hypothetical protein, at 1,827,610 to 1,828,500
_1565
Pf6N2E2_1566 - FIG00960770: hypothetical protein, at 1,828,497 to 1,829,270
_1566
Pf6N2E2_1567 - FIG00964846: hypothetical protein, at 1,829,310 to 1,830,194
_1567
Pf6N2E2_1568 - FIG00960890: hypothetical protein, at 1,830,191 to 1,831,420
_1568
Position (kb)
1828
1829
1830 Strain fitness (log2 ratio)
-1
0
1
2 at 1827.784 kb on + strand, within Pf6N2E2_1565 at 1828.034 kb on - strand, within Pf6N2E2_1565 at 1828.150 kb on + strand, within Pf6N2E2_1565 at 1828.150 kb on + strand, within Pf6N2E2_1565 at 1828.150 kb on + strand, within Pf6N2E2_1565 at 1828.151 kb on - strand, within Pf6N2E2_1565 at 1828.169 kb on - strand, within Pf6N2E2_1565 at 1828.169 kb on - strand, within Pf6N2E2_1565 at 1828.252 kb on + strand, within Pf6N2E2_1565 at 1828.252 kb on + strand, within Pf6N2E2_1565 at 1828.252 kb on + strand, within Pf6N2E2_1565 at 1828.253 kb on - strand, within Pf6N2E2_1565 at 1828.253 kb on - strand, within Pf6N2E2_1565 at 1828.315 kb on + strand, within Pf6N2E2_1565 at 1828.316 kb on - strand, within Pf6N2E2_1565 at 1828.363 kb on - strand, within Pf6N2E2_1565 at 1828.363 kb on - strand, within Pf6N2E2_1565 at 1828.442 kb on + strand at 1828.442 kb on + strand at 1828.512 kb on - strand at 1828.816 kb on + strand, within Pf6N2E2_1566 at 1828.816 kb on + strand, within Pf6N2E2_1566 at 1828.816 kb on + strand, within Pf6N2E2_1566 at 1828.817 kb on - strand, within Pf6N2E2_1566 at 1829.038 kb on + strand, within Pf6N2E2_1566 at 1829.038 kb on + strand, within Pf6N2E2_1566 at 1829.225 kb on - strand at 1829.319 kb on + strand at 1829.457 kb on + strand, within Pf6N2E2_1567 at 1829.457 kb on + strand, within Pf6N2E2_1567 at 1829.457 kb on + strand, within Pf6N2E2_1567 at 1829.457 kb on + strand, within Pf6N2E2_1567 at 1829.743 kb on + strand, within Pf6N2E2_1567 at 1829.743 kb on + strand, within Pf6N2E2_1567 at 1829.743 kb on + strand, within Pf6N2E2_1567 at 1829.744 kb on - strand, within Pf6N2E2_1567 at 1829.744 kb on - strand, within Pf6N2E2_1567 at 1829.744 kb on - strand, within Pf6N2E2_1567 at 1830.231 kb on - strand
Per-strain Table
Position Strand Gene LocusTag Fraction 10% P. fluorescens FW300-N2C3 spent media from growth in 20 mM putrescine remove 1,827,784 + Pf6N2E2_1565 0.20 +0.9 1,828,034 - Pf6N2E2_1565 0.48 +0.0 1,828,150 + Pf6N2E2_1565 0.61 +0.1 1,828,150 + Pf6N2E2_1565 0.61 -1.1 1,828,150 + Pf6N2E2_1565 0.61 -1.5 1,828,151 - Pf6N2E2_1565 0.61 -0.5 1,828,169 - Pf6N2E2_1565 0.63 +0.1 1,828,169 - Pf6N2E2_1565 0.63 +0.5 1,828,252 + Pf6N2E2_1565 0.72 -1.1 1,828,252 + Pf6N2E2_1565 0.72 +0.9 1,828,252 + Pf6N2E2_1565 0.72 -1.7 1,828,253 - Pf6N2E2_1565 0.72 +0.6 1,828,253 - Pf6N2E2_1565 0.72 -0.2 1,828,315 + Pf6N2E2_1565 0.79 +0.7 1,828,316 - Pf6N2E2_1565 0.79 -0.2 1,828,363 - Pf6N2E2_1565 0.85 -0.4 1,828,363 - Pf6N2E2_1565 0.85 -0.4 1,828,442 + +0.5 1,828,442 + +1.2 1,828,512 - -1.5 1,828,816 + Pf6N2E2_1566 0.41 -1.1 1,828,816 + Pf6N2E2_1566 0.41 -0.8 1,828,816 + Pf6N2E2_1566 0.41 -0.5 1,828,817 - Pf6N2E2_1566 0.41 -0.6 1,829,038 + Pf6N2E2_1566 0.70 -1.5 1,829,038 + Pf6N2E2_1566 0.70 +2.5 1,829,225 - +0.5 1,829,319 + +0.9 1,829,457 + Pf6N2E2_1567 0.17 +0.3 1,829,457 + Pf6N2E2_1567 0.17 +0.2 1,829,457 + Pf6N2E2_1567 0.17 -0.5 1,829,457 + Pf6N2E2_1567 0.17 +0.1 1,829,743 + Pf6N2E2_1567 0.49 +1.6 1,829,743 + Pf6N2E2_1567 0.49 +0.2 1,829,743 + Pf6N2E2_1567 0.49 +0.5 1,829,744 - Pf6N2E2_1567 0.49 -1.1 1,829,744 - Pf6N2E2_1567 0.49 -0.5 1,829,744 - Pf6N2E2_1567 0.49 -1.1 1,830,231 - +0.2
Or see this region's nucleotide sequence