Strain Fitness in Pseudomonas fluorescens FW300-N2E2 around Pf6N2E2_142

Experiment: 10% P. fluorescens FW300-N2C3 spent media from growth in 20 mM putrescine

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Only strains with sufficient reads to estimate fitness are shown, but the strain fitness values are still rather noisy and may be biased towards zero. Strains near the edge of a gene are not shown as being associated with that gene (they are in grey). Strains in the central 10-90% of a gene are color coded by the insertion's strand. Usually, "+" means that the selectable marker is encoded on the forward strand, i.e., transcribed rightward.

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500 ntPf6N2E2_141 and Pf6N2E2_142 are separated by 192 nucleotidesPf6N2E2_142 and Pf6N2E2_143 are separated by 177 nucleotidesPf6N2E2_143 and Pf6N2E2_144 are separated by 83 nucleotides Pf6N2E2_141 - Phosphate ABC transporter, periplasmic phosphate-binding protein PstS (TC 3.A.1.7.1), at 192,205 to 193,539 _141 Pf6N2E2_142 - Exodeoxyribonuclease III (EC 3.1.11.2), at 193,732 to 194,544 _142 Pf6N2E2_143 - Histone acetyltransferase HPA2 and related acetyltransferases, at 194,722 to 195,372 _143 Pf6N2E2_144 - Uncharacterized protein YtfM precursor, at 195,456 to 197,165 _144 Position (kb) 193 194 195Strain fitness (log2 ratio) -2 -1 0 1 2 3at 192.827 kb on + strand, within Pf6N2E2_141at 192.960 kb on + strand, within Pf6N2E2_141at 192.960 kb on + strand, within Pf6N2E2_141at 192.960 kb on + strand, within Pf6N2E2_141at 192.960 kb on + strand, within Pf6N2E2_141at 192.960 kb on + strand, within Pf6N2E2_141at 192.965 kb on + strand, within Pf6N2E2_141at 192.965 kb on + strand, within Pf6N2E2_141at 192.965 kb on + strand, within Pf6N2E2_141at 192.965 kb on + strand, within Pf6N2E2_141at 192.965 kb on + strand, within Pf6N2E2_141at 192.965 kb on + strand, within Pf6N2E2_141at 192.965 kb on + strand, within Pf6N2E2_141at 192.966 kb on - strand, within Pf6N2E2_141at 192.966 kb on - strand, within Pf6N2E2_141at 192.966 kb on - strand, within Pf6N2E2_141at 192.966 kb on - strand, within Pf6N2E2_141at 192.992 kb on + strand, within Pf6N2E2_141at 192.992 kb on + strand, within Pf6N2E2_141at 193.111 kb on - strand, within Pf6N2E2_141at 193.428 kb on + strandat 193.448 kb on - strandat 193.577 kb on + strandat 193.577 kb on + strandat 193.620 kb on + strandat 193.628 kb on + strandat 193.628 kb on + strandat 193.629 kb on - strandat 193.629 kb on - strandat 194.216 kb on + strand, within Pf6N2E2_142at 194.216 kb on + strand, within Pf6N2E2_142at 194.315 kb on + strand, within Pf6N2E2_142at 194.315 kb on + strand, within Pf6N2E2_142at 194.316 kb on - strand, within Pf6N2E2_142at 194.316 kb on - strand, within Pf6N2E2_142at 194.316 kb on - strand, within Pf6N2E2_142at 194.316 kb on - strand, within Pf6N2E2_142at 194.378 kb on + strand, within Pf6N2E2_142at 194.574 kb on + strandat 194.574 kb on + strandat 194.575 kb on - strandat 194.575 kb on - strandat 194.575 kb on - strandat 194.641 kb on + strandat 194.641 kb on + strandat 194.641 kb on + strandat 194.642 kb on - strandat 194.670 kb on + strandat 194.671 kb on - strandat 194.677 kb on + strandat 194.757 kb on + strandat 194.757 kb on + strandat 194.757 kb on + strandat 194.758 kb on - strandat 194.773 kb on + strandat 194.801 kb on - strand, within Pf6N2E2_143at 194.801 kb on - strand, within Pf6N2E2_143at 194.801 kb on - strand, within Pf6N2E2_143at 194.801 kb on - strand, within Pf6N2E2_143at 194.833 kb on + strand, within Pf6N2E2_143at 194.833 kb on + strand, within Pf6N2E2_143at 194.834 kb on - strand, within Pf6N2E2_143at 194.834 kb on - strand, within Pf6N2E2_143at 194.834 kb on - strand, within Pf6N2E2_143at 194.834 kb on - strand, within Pf6N2E2_143at 195.074 kb on - strand, within Pf6N2E2_143at 195.074 kb on - strand, within Pf6N2E2_143at 195.119 kb on + strand, within Pf6N2E2_143at 195.119 kb on + strand, within Pf6N2E2_143at 195.119 kb on + strand, within Pf6N2E2_143at 195.119 kb on + strand, within Pf6N2E2_143at 195.119 kb on + strand, within Pf6N2E2_143at 195.120 kb on - strand, within Pf6N2E2_143at 195.120 kb on - strand, within Pf6N2E2_143at 195.120 kb on - strand, within Pf6N2E2_143at 195.192 kb on + strand, within Pf6N2E2_143at 195.192 kb on + strand, within Pf6N2E2_143at 195.192 kb on + strand, within Pf6N2E2_143at 195.192 kb on + strand, within Pf6N2E2_143at 195.192 kb on + strand, within Pf6N2E2_143at 195.192 kb on + strand, within Pf6N2E2_143at 195.193 kb on - strand, within Pf6N2E2_143at 195.193 kb on - strand, within Pf6N2E2_143at 195.193 kb on - strand, within Pf6N2E2_143at 195.254 kb on - strand, within Pf6N2E2_143at 195.254 kb on - strand, within Pf6N2E2_143at 195.254 kb on - strand, within Pf6N2E2_143at 195.254 kb on - strand, within Pf6N2E2_143at 195.265 kb on + strand, within Pf6N2E2_143at 195.266 kb on - strand, within Pf6N2E2_143at 195.268 kb on - strand, within Pf6N2E2_143at 195.281 kb on - strand, within Pf6N2E2_143at 195.371 kb on - strandat 195.414 kb on + strandat 195.520 kb on + strandat 195.520 kb on + strandat 195.521 kb on - strandat 195.521 kb on - strand

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Per-strain Table

Position Strand Gene LocusTag Fraction 10% P. fluorescens FW300-N2C3 spent media from growth in 20 mM putrescine
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192,827 + Pf6N2E2_141 0.47 -0.4
192,960 + Pf6N2E2_141 0.57 +0.4
192,960 + Pf6N2E2_141 0.57 -0.0
192,960 + Pf6N2E2_141 0.57 +0.2
192,960 + Pf6N2E2_141 0.57 -1.7
192,960 + Pf6N2E2_141 0.57 +0.3
192,965 + Pf6N2E2_141 0.57 -0.3
192,965 + Pf6N2E2_141 0.57 +1.1
192,965 + Pf6N2E2_141 0.57 -1.5
192,965 + Pf6N2E2_141 0.57 -1.4
192,965 + Pf6N2E2_141 0.57 +0.2
192,965 + Pf6N2E2_141 0.57 -0.2
192,965 + Pf6N2E2_141 0.57 +0.3
192,966 - Pf6N2E2_141 0.57 +0.0
192,966 - Pf6N2E2_141 0.57 +0.1
192,966 - Pf6N2E2_141 0.57 +0.6
192,966 - Pf6N2E2_141 0.57 -0.4
192,992 + Pf6N2E2_141 0.59 +0.3
192,992 + Pf6N2E2_141 0.59 -0.0
193,111 - Pf6N2E2_141 0.68 -0.2
193,428 + +1.1
193,448 - +0.4
193,577 + -0.2
193,577 + -0.2
193,620 + +0.7
193,628 + -0.5
193,628 + -0.7
193,629 - +0.3
193,629 - +0.8
194,216 + Pf6N2E2_142 0.60 -0.2
194,216 + Pf6N2E2_142 0.60 -0.2
194,315 + Pf6N2E2_142 0.72 +0.8
194,315 + Pf6N2E2_142 0.72 -1.7
194,316 - Pf6N2E2_142 0.72 +1.6
194,316 - Pf6N2E2_142 0.72 -1.0
194,316 - Pf6N2E2_142 0.72 -1.0
194,316 - Pf6N2E2_142 0.72 -1.0
194,378 + Pf6N2E2_142 0.79 -2.2
194,574 + -0.2
194,574 + -0.0
194,575 - +0.8
194,575 - +0.8
194,575 - +0.5
194,641 + -0.2
194,641 + -0.7
194,641 + -0.4
194,642 - +0.7
194,670 + +0.4
194,671 - -0.2
194,677 + -0.6
194,757 + -0.2
194,757 + -0.2
194,757 + +0.1
194,758 - -0.4
194,773 + +0.4
194,801 - Pf6N2E2_143 0.12 -0.5
194,801 - Pf6N2E2_143 0.12 +0.8
194,801 - Pf6N2E2_143 0.12 +1.2
194,801 - Pf6N2E2_143 0.12 +3.5
194,833 + Pf6N2E2_143 0.17 +0.8
194,833 + Pf6N2E2_143 0.17 -0.2
194,834 - Pf6N2E2_143 0.17 +0.8
194,834 - Pf6N2E2_143 0.17 +2.2
194,834 - Pf6N2E2_143 0.17 +1.4
194,834 - Pf6N2E2_143 0.17 +0.4
195,074 - Pf6N2E2_143 0.54 +0.4
195,074 - Pf6N2E2_143 0.54 -0.2
195,119 + Pf6N2E2_143 0.61 +0.2
195,119 + Pf6N2E2_143 0.61 +0.5
195,119 + Pf6N2E2_143 0.61 -2.2
195,119 + Pf6N2E2_143 0.61 -0.1
195,119 + Pf6N2E2_143 0.61 +1.2
195,120 - Pf6N2E2_143 0.61 -0.2
195,120 - Pf6N2E2_143 0.61 +0.6
195,120 - Pf6N2E2_143 0.61 +0.5
195,192 + Pf6N2E2_143 0.72 +0.6
195,192 + Pf6N2E2_143 0.72 +0.4
195,192 + Pf6N2E2_143 0.72 -0.2
195,192 + Pf6N2E2_143 0.72 +0.9
195,192 + Pf6N2E2_143 0.72 +1.6
195,192 + Pf6N2E2_143 0.72 -0.8
195,193 - Pf6N2E2_143 0.72 +0.4
195,193 - Pf6N2E2_143 0.72 +1.8
195,193 - Pf6N2E2_143 0.72 +0.3
195,254 - Pf6N2E2_143 0.82 +0.3
195,254 - Pf6N2E2_143 0.82 +1.8
195,254 - Pf6N2E2_143 0.82 +0.3
195,254 - Pf6N2E2_143 0.82 +1.0
195,265 + Pf6N2E2_143 0.83 +0.4
195,266 - Pf6N2E2_143 0.84 +1.4
195,268 - Pf6N2E2_143 0.84 -1.2
195,281 - Pf6N2E2_143 0.86 -0.2
195,371 - +0.4
195,414 + -1.7
195,520 + +0.6
195,520 + +0.6
195,521 - +2.2
195,521 - +0.8

Or see this region's nucleotide sequence