Experiment: 10% P. fluorescens FW300-N2C3 spent media from growth in 20 mM putrescine
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt Pf6N2E2_1075 and Pf6N2E2_1076 overlap by 4 nucleotides Pf6N2E2_1076 and Pf6N2E2_1077 overlap by 4 nucleotides
Pf6N2E2_1075 - Malto-oligosyltrehalose synthase (EC 5.4.99.15), at 1,254,640 to 1,257,426
_1075
Pf6N2E2_1076 - 4-alpha-glucanotransferase (amylomaltase) (EC 2.4.1.25), at 1,257,423 to 1,259,501
_1076
Pf6N2E2_1077 - Malto-oligosyltrehalose trehalohydrolase (EC 3.2.1.141), at 1,259,498 to 1,261,258
_1077
Position (kb)
1257
1258
1259
1260 Strain fitness (log2 ratio)
-1
0
1
2 at 1256.522 kb on - strand, within Pf6N2E2_1075 at 1256.681 kb on + strand, within Pf6N2E2_1075 at 1256.787 kb on - strand, within Pf6N2E2_1075 at 1256.921 kb on + strand, within Pf6N2E2_1075 at 1256.922 kb on - strand, within Pf6N2E2_1075 at 1257.068 kb on + strand, within Pf6N2E2_1075 at 1257.068 kb on + strand, within Pf6N2E2_1075 at 1257.169 kb on - strand at 1257.302 kb on + strand at 1257.302 kb on + strand at 1257.302 kb on + strand at 1257.302 kb on + strand at 1257.303 kb on - strand at 1257.465 kb on + strand at 1258.072 kb on + strand, within Pf6N2E2_1076 at 1258.072 kb on + strand, within Pf6N2E2_1076 at 1258.072 kb on + strand, within Pf6N2E2_1076 at 1258.073 kb on - strand, within Pf6N2E2_1076 at 1258.457 kb on - strand, within Pf6N2E2_1076 at 1258.874 kb on - strand, within Pf6N2E2_1076 at 1258.874 kb on - strand, within Pf6N2E2_1076 at 1259.023 kb on + strand, within Pf6N2E2_1076 at 1259.023 kb on + strand, within Pf6N2E2_1076 at 1259.024 kb on - strand, within Pf6N2E2_1076 at 1259.024 kb on - strand, within Pf6N2E2_1076 at 1259.024 kb on - strand, within Pf6N2E2_1076 at 1259.087 kb on - strand, within Pf6N2E2_1076 at 1259.251 kb on + strand, within Pf6N2E2_1076 at 1259.252 kb on - strand, within Pf6N2E2_1076 at 1259.252 kb on - strand, within Pf6N2E2_1076 at 1260.005 kb on - strand, within Pf6N2E2_1077 at 1260.071 kb on + strand, within Pf6N2E2_1077 at 1260.307 kb on - strand, within Pf6N2E2_1077 at 1260.315 kb on + strand, within Pf6N2E2_1077 at 1260.315 kb on + strand, within Pf6N2E2_1077 at 1260.318 kb on + strand, within Pf6N2E2_1077 at 1260.319 kb on - strand, within Pf6N2E2_1077 at 1260.387 kb on + strand, within Pf6N2E2_1077 at 1260.387 kb on + strand, within Pf6N2E2_1077 at 1260.388 kb on - strand, within Pf6N2E2_1077
Per-strain Table
Position Strand Gene LocusTag Fraction 10% P. fluorescens FW300-N2C3 spent media from growth in 20 mM putrescine remove 1,256,522 - Pf6N2E2_1075 0.68 -0.6 1,256,681 + Pf6N2E2_1075 0.73 +0.3 1,256,787 - Pf6N2E2_1075 0.77 +2.0 1,256,921 + Pf6N2E2_1075 0.82 +1.0 1,256,922 - Pf6N2E2_1075 0.82 +0.2 1,257,068 + Pf6N2E2_1075 0.87 +0.5 1,257,068 + Pf6N2E2_1075 0.87 -0.4 1,257,169 - -0.6 1,257,302 + -0.2 1,257,302 + -0.6 1,257,302 + -0.3 1,257,302 + -0.7 1,257,303 - -0.8 1,257,465 + -0.4 1,258,072 + Pf6N2E2_1076 0.31 -1.1 1,258,072 + Pf6N2E2_1076 0.31 -0.6 1,258,072 + Pf6N2E2_1076 0.31 -0.6 1,258,073 - Pf6N2E2_1076 0.31 -0.6 1,258,457 - Pf6N2E2_1076 0.50 +0.4 1,258,874 - Pf6N2E2_1076 0.70 -1.6 1,258,874 - Pf6N2E2_1076 0.70 -0.6 1,259,023 + Pf6N2E2_1076 0.77 +0.4 1,259,023 + Pf6N2E2_1076 0.77 -0.7 1,259,024 - Pf6N2E2_1076 0.77 +0.4 1,259,024 - Pf6N2E2_1076 0.77 -0.6 1,259,024 - Pf6N2E2_1076 0.77 -0.6 1,259,087 - Pf6N2E2_1076 0.80 -0.2 1,259,251 + Pf6N2E2_1076 0.88 -0.6 1,259,252 - Pf6N2E2_1076 0.88 -1.6 1,259,252 - Pf6N2E2_1076 0.88 -0.6 1,260,005 - Pf6N2E2_1077 0.29 +0.6 1,260,071 + Pf6N2E2_1077 0.33 -0.4 1,260,307 - Pf6N2E2_1077 0.46 +0.2 1,260,315 + Pf6N2E2_1077 0.46 -0.5 1,260,315 + Pf6N2E2_1077 0.46 +1.4 1,260,318 + Pf6N2E2_1077 0.47 -0.6 1,260,319 - Pf6N2E2_1077 0.47 +1.6 1,260,387 + Pf6N2E2_1077 0.50 +1.0 1,260,387 + Pf6N2E2_1077 0.50 -0.6 1,260,388 - Pf6N2E2_1077 0.51 +0.5
Or see this region's nucleotide sequence