Strain Fitness in Pseudomonas fluorescens FW300-N2E2 around Pf6N2E2_1076

Experiment: 10% P. fluorescens FW300-N2C3 spent media from growth in 20 mM putrescine

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Only strains with sufficient reads to estimate fitness are shown, but the strain fitness values are still rather noisy and may be biased towards zero. Strains near the edge of a gene are not shown as being associated with that gene (they are in grey). Strains in the central 10-90% of a gene are color coded by the insertion's strand. Usually, "+" means that the selectable marker is encoded on the forward strand, i.e., transcribed rightward.

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500 ntPf6N2E2_1075 and Pf6N2E2_1076 overlap by 4 nucleotidesPf6N2E2_1076 and Pf6N2E2_1077 overlap by 4 nucleotides Pf6N2E2_1075 - Malto-oligosyltrehalose synthase (EC 5.4.99.15), at 1,254,640 to 1,257,426 _1075 Pf6N2E2_1076 - 4-alpha-glucanotransferase (amylomaltase) (EC 2.4.1.25), at 1,257,423 to 1,259,501 _1076 Pf6N2E2_1077 - Malto-oligosyltrehalose trehalohydrolase (EC 3.2.1.141), at 1,259,498 to 1,261,258 _1077 Position (kb) 1257 1258 1259 1260Strain fitness (log2 ratio) -1 0 1 2at 1256.522 kb on - strand, within Pf6N2E2_1075at 1256.681 kb on + strand, within Pf6N2E2_1075at 1256.787 kb on - strand, within Pf6N2E2_1075at 1256.921 kb on + strand, within Pf6N2E2_1075at 1256.922 kb on - strand, within Pf6N2E2_1075at 1257.068 kb on + strand, within Pf6N2E2_1075at 1257.068 kb on + strand, within Pf6N2E2_1075at 1257.169 kb on - strandat 1257.302 kb on + strandat 1257.302 kb on + strandat 1257.302 kb on + strandat 1257.302 kb on + strandat 1257.303 kb on - strandat 1257.465 kb on + strandat 1258.072 kb on + strand, within Pf6N2E2_1076at 1258.072 kb on + strand, within Pf6N2E2_1076at 1258.072 kb on + strand, within Pf6N2E2_1076at 1258.073 kb on - strand, within Pf6N2E2_1076at 1258.457 kb on - strand, within Pf6N2E2_1076at 1258.874 kb on - strand, within Pf6N2E2_1076at 1258.874 kb on - strand, within Pf6N2E2_1076at 1259.023 kb on + strand, within Pf6N2E2_1076at 1259.023 kb on + strand, within Pf6N2E2_1076at 1259.024 kb on - strand, within Pf6N2E2_1076at 1259.024 kb on - strand, within Pf6N2E2_1076at 1259.024 kb on - strand, within Pf6N2E2_1076at 1259.087 kb on - strand, within Pf6N2E2_1076at 1259.251 kb on + strand, within Pf6N2E2_1076at 1259.252 kb on - strand, within Pf6N2E2_1076at 1259.252 kb on - strand, within Pf6N2E2_1076at 1260.005 kb on - strand, within Pf6N2E2_1077at 1260.071 kb on + strand, within Pf6N2E2_1077at 1260.307 kb on - strand, within Pf6N2E2_1077at 1260.315 kb on + strand, within Pf6N2E2_1077at 1260.315 kb on + strand, within Pf6N2E2_1077at 1260.318 kb on + strand, within Pf6N2E2_1077at 1260.319 kb on - strand, within Pf6N2E2_1077at 1260.387 kb on + strand, within Pf6N2E2_1077at 1260.387 kb on + strand, within Pf6N2E2_1077at 1260.388 kb on - strand, within Pf6N2E2_1077

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Per-strain Table

Position Strand Gene LocusTag Fraction 10% P. fluorescens FW300-N2C3 spent media from growth in 20 mM putrescine
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1,256,522 - Pf6N2E2_1075 0.68 -0.6
1,256,681 + Pf6N2E2_1075 0.73 +0.3
1,256,787 - Pf6N2E2_1075 0.77 +2.0
1,256,921 + Pf6N2E2_1075 0.82 +1.0
1,256,922 - Pf6N2E2_1075 0.82 +0.2
1,257,068 + Pf6N2E2_1075 0.87 +0.5
1,257,068 + Pf6N2E2_1075 0.87 -0.4
1,257,169 - -0.6
1,257,302 + -0.2
1,257,302 + -0.6
1,257,302 + -0.3
1,257,302 + -0.7
1,257,303 - -0.8
1,257,465 + -0.4
1,258,072 + Pf6N2E2_1076 0.31 -1.1
1,258,072 + Pf6N2E2_1076 0.31 -0.6
1,258,072 + Pf6N2E2_1076 0.31 -0.6
1,258,073 - Pf6N2E2_1076 0.31 -0.6
1,258,457 - Pf6N2E2_1076 0.50 +0.4
1,258,874 - Pf6N2E2_1076 0.70 -1.6
1,258,874 - Pf6N2E2_1076 0.70 -0.6
1,259,023 + Pf6N2E2_1076 0.77 +0.4
1,259,023 + Pf6N2E2_1076 0.77 -0.7
1,259,024 - Pf6N2E2_1076 0.77 +0.4
1,259,024 - Pf6N2E2_1076 0.77 -0.6
1,259,024 - Pf6N2E2_1076 0.77 -0.6
1,259,087 - Pf6N2E2_1076 0.80 -0.2
1,259,251 + Pf6N2E2_1076 0.88 -0.6
1,259,252 - Pf6N2E2_1076 0.88 -1.6
1,259,252 - Pf6N2E2_1076 0.88 -0.6
1,260,005 - Pf6N2E2_1077 0.29 +0.6
1,260,071 + Pf6N2E2_1077 0.33 -0.4
1,260,307 - Pf6N2E2_1077 0.46 +0.2
1,260,315 + Pf6N2E2_1077 0.46 -0.5
1,260,315 + Pf6N2E2_1077 0.46 +1.4
1,260,318 + Pf6N2E2_1077 0.47 -0.6
1,260,319 - Pf6N2E2_1077 0.47 +1.6
1,260,387 + Pf6N2E2_1077 0.50 +1.0
1,260,387 + Pf6N2E2_1077 0.50 -0.6
1,260,388 - Pf6N2E2_1077 0.51 +0.5

Or see this region's nucleotide sequence