Experiment: CDM1Gut with 50% spent media from Escherichia fergusonnii TT2
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt BBR_RS13105 and BBR_RS13110 are separated by 93 nucleotides BBR_RS13110 and BBR_RS13115 are separated by 126 nucleotides BBR_RS13115 and BBR_RS13120 are separated by 186 nucleotides
BBR_RS13105: BBR_RS13105 - sugar ABC transporter substrate-binding protein, at 715,037 to 716,458
_RS13105
BBR_RS13110: BBR_RS13110 - type III pantothenate kinase, at 716,552 to 717,322
_RS13110
BBR_RS13115: BBR_RS13115 - Solute-binding protein of ABC transporter system for peptide, at 717,449 to 719,083
_RS13115
BBR_RS13120: BBR_RS13120 - ABC transporter permease, at 719,270 to 720,247
_RS13120
Position (kb) 717
718
719
720 Strain fitness (log2 ratio) -3
-2
-1
0
1
2
3
4 at 716.474 kb on - strand at 716.527 kb on - strand at 716.527 kb on - strand at 716.604 kb on + strand at 716.639 kb on - strand, within BBR_RS13110 at 716.670 kb on + strand, within BBR_RS13110 at 716.671 kb on + strand, within BBR_RS13110 at 716.737 kb on - strand, within BBR_RS13110 at 716.737 kb on - strand, within BBR_RS13110 at 716.739 kb on + strand, within BBR_RS13110 at 716.739 kb on + strand, within BBR_RS13110 at 716.739 kb on + strand, within BBR_RS13110 at 716.747 kb on - strand, within BBR_RS13110 at 716.765 kb on + strand, within BBR_RS13110 at 716.842 kb on + strand, within BBR_RS13110 at 716.901 kb on - strand, within BBR_RS13110 at 716.903 kb on - strand, within BBR_RS13110 at 716.970 kb on - strand, within BBR_RS13110 at 717.031 kb on + strand, within BBR_RS13110 at 717.039 kb on - strand, within BBR_RS13110 at 717.039 kb on - strand, within BBR_RS13110 at 717.099 kb on - strand, within BBR_RS13110 at 717.117 kb on + strand, within BBR_RS13110 at 717.117 kb on + strand, within BBR_RS13110 at 717.117 kb on + strand, within BBR_RS13110 at 717.118 kb on - strand, within BBR_RS13110 at 717.125 kb on - strand, within BBR_RS13110 at 717.126 kb on - strand, within BBR_RS13110 at 717.135 kb on - strand, within BBR_RS13110 at 717.163 kb on + strand, within BBR_RS13110 at 717.171 kb on - strand, within BBR_RS13110 at 717.216 kb on + strand, within BBR_RS13110 at 717.218 kb on + strand, within BBR_RS13110 at 717.388 kb on + strand at 717.391 kb on - strand at 717.391 kb on - strand at 717.396 kb on - strand at 717.396 kb on - strand at 717.984 kb on - strand, within BBR_RS13115 at 718.399 kb on - strand, within BBR_RS13115 at 718.478 kb on - strand, within BBR_RS13115 at 719.030 kb on + strand at 719.030 kb on + strand at 719.048 kb on - strand at 719.049 kb on - strand at 719.050 kb on + strand at 719.050 kb on + strand at 719.083 kb on - strand at 719.125 kb on - strand at 719.157 kb on + strand at 719.164 kb on - strand at 719.165 kb on - strand at 719.175 kb on - strand at 719.200 kb on + strand at 719.202 kb on + strand at 719.202 kb on + strand at 719.204 kb on + strand at 719.223 kb on + strand at 719.238 kb on + strand at 719.248 kb on + strand
Per-strain Table
Position Strand Gene LocusTag Fraction CDM1Gut with 50% spent media from Escherichia fergusonnii TT2 remove 716,474 - -0.2 716,527 - +1.4 716,527 - -3.1 716,604 + +0.4 716,639 - BBR_RS13110 0.11 +2.4 716,670 + BBR_RS13110 0.15 +0.9 716,671 + BBR_RS13110 0.15 -0.6 716,737 - BBR_RS13110 0.24 -0.6 716,737 - BBR_RS13110 0.24 -0.4 716,739 + BBR_RS13110 0.24 -0.4 716,739 + BBR_RS13110 0.24 +0.4 716,739 + BBR_RS13110 0.24 +3.7 716,747 - BBR_RS13110 0.25 -0.2 716,765 + BBR_RS13110 0.28 +2.2 716,842 + BBR_RS13110 0.38 +0.5 716,901 - BBR_RS13110 0.45 +4.2 716,903 - BBR_RS13110 0.46 +1.3 716,970 - BBR_RS13110 0.54 +2.9 717,031 + BBR_RS13110 0.62 -0.4 717,039 - BBR_RS13110 0.63 -1.9 717,039 - BBR_RS13110 0.63 -0.4 717,099 - BBR_RS13110 0.71 +2.1 717,117 + BBR_RS13110 0.73 +0.7 717,117 + BBR_RS13110 0.73 +0.4 717,117 + BBR_RS13110 0.73 -0.6 717,118 - BBR_RS13110 0.73 +0.6 717,125 - BBR_RS13110 0.74 -1.6 717,126 - BBR_RS13110 0.74 -0.9 717,135 - BBR_RS13110 0.76 -0.9 717,163 + BBR_RS13110 0.79 +2.5 717,171 - BBR_RS13110 0.80 +0.4 717,216 + BBR_RS13110 0.86 +0.4 717,218 + BBR_RS13110 0.86 -1.2 717,388 + -2.4 717,391 - -0.9 717,391 - -0.9 717,396 - -0.1 717,396 - -0.5 717,984 - BBR_RS13115 0.33 +1.9 718,399 - BBR_RS13115 0.58 +0.9 718,478 - BBR_RS13115 0.63 +1.9 719,030 + -2.0 719,030 + +4.4 719,048 - -1.9 719,049 - -0.9 719,050 + -0.8 719,050 + -0.5 719,083 - -2.0 719,125 - -0.2 719,157 + +3.3 719,164 - +1.0 719,165 - +1.5 719,175 - -1.2 719,200 + +0.9 719,202 + +1.1 719,202 + -1.5 719,204 + +1.9 719,223 + -0.1 719,238 + +0.5 719,248 + -2.6
Or see this region's nucleotide sequence