Experiment: MoLS4 with 2-oxobutyrate 10mM
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt nrfA and ilvN-1 are separated by 432 nucleotides ilvN-1 and ptB are separated by 3 nucleotides ptB and buk overlap by 4 nucleotides
DVU0625: nrfA - cytochrome c nitrite reductase, catalytic subunit NrfA, putative (Shelley Haveman), at 691,544 to 693,118
nrfA
DVU0626: ilvN-1 - acetolactate synthase, small subunit (TIGR), at 693,551 to 694,030
ilvN-1
DVU0627: ptB - phosphotransbutyrylase (TIGR), at 694,034 to 695,065
ptB
DVU0628: buk - butyrate kinase (TIGR), at 695,062 to 696,180
buk
Position (kb) 694
695
696 Strain fitness (log2 ratio) -4
-3
-2
-1
0
1 at 693.037 kb on - strand at 693.061 kb on + strand at 693.061 kb on + strand at 693.069 kb on - strand at 693.069 kb on - strand at 693.069 kb on - strand at 693.069 kb on - strand at 693.069 kb on - strand at 693.069 kb on - strand at 693.069 kb on - strand at 693.076 kb on + strand at 693.093 kb on + strand at 693.095 kb on + strand at 693.103 kb on - strand at 693.140 kb on + strand at 693.148 kb on - strand at 693.185 kb on + strand at 693.295 kb on + strand at 693.301 kb on - strand at 693.312 kb on + strand at 693.330 kb on - strand at 693.332 kb on + strand at 693.353 kb on + strand at 693.479 kb on + strand at 693.506 kb on - strand at 693.513 kb on + strand at 693.521 kb on - strand at 693.530 kb on + strand at 693.616 kb on - strand, within ilvN-1 at 693.628 kb on - strand, within ilvN-1 at 693.735 kb on - strand, within ilvN-1 at 693.814 kb on + strand, within ilvN-1 at 693.814 kb on + strand, within ilvN-1 at 693.814 kb on + strand, within ilvN-1 at 693.822 kb on - strand, within ilvN-1 at 693.822 kb on - strand, within ilvN-1 at 693.822 kb on - strand, within ilvN-1 at 693.822 kb on - strand, within ilvN-1 at 693.822 kb on - strand, within ilvN-1 at 693.822 kb on - strand, within ilvN-1 at 693.822 kb on - strand, within ilvN-1 at 693.904 kb on + strand, within ilvN-1 at 693.947 kb on + strand, within ilvN-1 at 693.984 kb on + strand at 693.984 kb on + strand at 694.051 kb on - strand at 694.085 kb on - strand at 694.113 kb on + strand at 694.231 kb on - strand, within ptB at 694.294 kb on - strand, within ptB at 694.361 kb on + strand, within ptB at 694.414 kb on - strand, within ptB at 694.423 kb on + strand, within ptB at 694.454 kb on - strand, within ptB at 694.537 kb on + strand, within ptB at 694.593 kb on - strand, within ptB at 694.609 kb on + strand, within ptB at 694.647 kb on + strand, within ptB at 694.738 kb on - strand, within ptB at 694.797 kb on + strand, within ptB at 694.805 kb on - strand, within ptB at 694.839 kb on - strand, within ptB at 694.897 kb on + strand, within ptB at 695.151 kb on - strand at 695.242 kb on - strand, within buk at 695.259 kb on - strand, within buk at 695.308 kb on + strand, within buk at 695.396 kb on - strand, within buk at 695.422 kb on + strand, within buk at 695.460 kb on + strand, within buk at 695.614 kb on + strand, within buk at 695.891 kb on - strand, within buk at 695.922 kb on + strand, within buk at 695.965 kb on + strand, within buk at 695.965 kb on + strand, within buk at 696.005 kb on + strand, within buk
Per-strain Table
Position Strand Gene LocusTag Fraction MoLS4 with 2-oxobutyrate 10mM remove 693,037 - -0.6 693,061 + -0.1 693,061 + +1.1 693,069 - +0.2 693,069 - -0.4 693,069 - +0.1 693,069 - -0.6 693,069 - +0.0 693,069 - +0.6 693,069 - -0.2 693,076 + +0.3 693,093 + -0.2 693,095 + -0.2 693,103 - +0.1 693,140 + -0.7 693,148 - +0.1 693,185 + +0.2 693,295 + -0.2 693,301 - +1.0 693,312 + -0.1 693,330 - -0.6 693,332 + -0.5 693,353 + +0.2 693,479 + -1.0 693,506 - -0.6 693,513 + -0.2 693,521 - -0.1 693,530 + +0.4 693,616 - ilvN-1 DVU0626 0.14 -0.8 693,628 - ilvN-1 DVU0626 0.16 -1.4 693,735 - ilvN-1 DVU0626 0.38 -2.6 693,814 + ilvN-1 DVU0626 0.55 -1.0 693,814 + ilvN-1 DVU0626 0.55 -0.5 693,814 + ilvN-1 DVU0626 0.55 -0.1 693,822 - ilvN-1 DVU0626 0.56 -0.8 693,822 - ilvN-1 DVU0626 0.56 -0.9 693,822 - ilvN-1 DVU0626 0.56 -0.4 693,822 - ilvN-1 DVU0626 0.56 -1.4 693,822 - ilvN-1 DVU0626 0.56 -0.1 693,822 - ilvN-1 DVU0626 0.56 -1.1 693,822 - ilvN-1 DVU0626 0.56 -0.3 693,904 + ilvN-1 DVU0626 0.74 -0.6 693,947 + ilvN-1 DVU0626 0.82 -0.8 693,984 + -0.2 693,984 + -0.4 694,051 - -2.9 694,085 - -2.6 694,113 + -2.3 694,231 - ptB DVU0627 0.19 -3.4 694,294 - ptB DVU0627 0.25 -2.8 694,361 + ptB DVU0627 0.32 -2.6 694,414 - ptB DVU0627 0.37 -3.3 694,423 + ptB DVU0627 0.38 -3.6 694,454 - ptB DVU0627 0.41 -0.4 694,537 + ptB DVU0627 0.49 -2.9 694,593 - ptB DVU0627 0.54 -4.1 694,609 + ptB DVU0627 0.56 -3.9 694,647 + ptB DVU0627 0.59 +0.0 694,738 - ptB DVU0627 0.68 -2.6 694,797 + ptB DVU0627 0.74 -4.1 694,805 - ptB DVU0627 0.75 -1.8 694,839 - ptB DVU0627 0.78 -3.3 694,897 + ptB DVU0627 0.84 -3.7 695,151 - -0.4 695,242 - buk DVU0628 0.16 -0.4 695,259 - buk DVU0628 0.18 +0.4 695,308 + buk DVU0628 0.22 -0.7 695,396 - buk DVU0628 0.30 -0.6 695,422 + buk DVU0628 0.32 -1.0 695,460 + buk DVU0628 0.36 -0.6 695,614 + buk DVU0628 0.49 -1.4 695,891 - buk DVU0628 0.74 -0.6 695,922 + buk DVU0628 0.77 -1.0 695,965 + buk DVU0628 0.81 -2.3 695,965 + buk DVU0628 0.81 -0.2 696,005 + buk DVU0628 0.84 +1.5
Or see this region's nucleotide sequence